Prognosis of Eight Chinese Cases of Primary Vaginal Yolk Sac Tumor with a Review of the Literature

Tang, Qiong-Lan,Jiang, Xue-Feng,Yuan, Xiao-Ping,Liu, Yong,Zhang, Lin,Tang, Xiao-Feng,Zhou, Jia-Jia,Li, Hai-Gang,Fang, Jian-Pei,Xue, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Primary vaginal yolk sac tumor is a rare malignancy in the pediatric population, and a diagnostic challenge and appropriate initial treatment remains unsolved. The aim of this study was to investigate the clinicopathologic features, treatment and prognosis of this tumor. Materials and Methods: Eight cases of primary vaginal yolk sac tumor were reported with a literature review. Results: There were 4 pure yolk sac tumor cases and four mixed germ cell tumors containing yolk sac tumor element, including two cases with embryonal carcinoma and two cases with embryonal carcinoma and dysgerminoma. Partial vaginectomy was performed in four cases and all patients received chemotherapy. 85 cases in literatures were reviewed and 9 cases were misdiagnosed. Follow-up data was available in 77 cases and 5-year overall survival rate was 87.6%. 5-year survival rate of biopsy with chemotherapy, conservative surgery with chemotherapy and radical surgery with chemotherapy was 91.1%, 100% and 28.6%, respectively (p<0.001). Compared to cases without relapse or metastasis after initial treatment, patients with relapse or metastasis had a shorter overall survival (35.6% vs 96.6%, p<0.001). Conclusions: Mixed germ cell tumor containing yolk sac tumor element was not uncommon and partial vaginectomy may be a good choice for primary vaginal mixed yolk sac tumor type to eradicate local tumor cells and provide complete information for pathological diagnosis and postoperative adjuvant therapy.

Effects of Gastric Cancer Cells on the Differentiation of Treg Cells

Hu, Jing-Lan,Yang, Zhen,Tang, Jian-Rong,Fu, Xue-Qin,Yao, Lan-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

The aim of this study was evaluated the prevalence of Treg cells in peripheral blood in patients with gastric cancer, and investigate the effect of gastric cancer cells on their differentiation. ELISA was employed to assess the concentrations of TGF-${\beta}$ and IL-10 in gastric cancer patients' serum. Then, mouse gastric cancer cells were co-cultured with T lymphocytes or T lymphocytes + anti-TGF-${\beta}$. Flow cytometric analysis and RT-PCR were then performed to detect Treg cells and TGF-${\beta}$ and IL-10 expression in gastric cancer cells. Our data showed that the expression of TGF-${\beta}$ and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors. The population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture group were much higher than in the control group (18.6% vs 9.5%) (P<0.05). Moreover, the population of Treg cells and the expression levels of TGF-${\beta}$ and IL-10 in the co-culture systerm were clearly decreased after addition of anti-TGF-${\beta}$ (7.7% vs 19.6%) (P<0.01). In conclusion, gastric cancer cells may induce Treg cell differentiation through TGF-${\beta}$, and further promote immunosuppression.

Compound heterozygous mutations of NDUFV1 identified in a child with mitochondrial complex I deficiency

Tang Xiaojun,Xu Wuhen,Song Xiaozhen,Ye Haiyun,Ren Xiang,Yang Yongchen,Zhang Hong,Wu Shengnan,Lan Xiaoping 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Mitochondrial complex I deficiency (MCID) is the most common biochemical defect identified in childhood with mitochondrial diseases, mainly including Leigh syndrome, encephalopathy, macrocephaly with progressive leukodystrophy, hypertrophic cardiomyopathy and myopathy. Objective: To identify genetic cause in a patient with early onset autosomal recessive MCID. Methods: Trio whole-exome sequencing was performed and phenotype-related data analyses were conducted. All candidate mutations were confirmed by Sanger sequencing. Results: Here we report a child of Leigh syndrome presented with global developmental delay, progressive muscular hypotonia and myocardial damage. A missense mutation c.118C > T (p.Arg40Trp) and a previously reported mutation c.1157G > A (p.Arg386His) in NDUFV1 have been identified as compound heterozygous in the patient. The mutation p.Arg386His is closely associated with the impairment of 4Fe-4S domain and this mutation has been reported pathogenic. The c.118C > T mutation has not been reported in ClinVar and HGMD database. In silico protein analyses showed that p.Arg40 is highly conserved in a wide range of species, and the amino acid substitution p.Trp40 largely decreases the stability of NDUFV1. In addition, the mutation has not been detected in the Asian populations and it was predicted to be deleterious by numerous prediction tools. Conclusion: This research expands the mutation spectrum of NDUFV1 and substantially provides an early and accurate diagnosis basis of MCID, which would benefit subsequently effective genetic counseling and prenatal diagnosis for future reproduction of the family.

Effects of Garlic Oil on Pancreatic Cancer Cells

Lan, X.Y.,Sun, H.Y.,Liu, J.J.,Lin, Y.,Zhu, Z.Y.,Han, X.,Sun, X.,Li, X.R.,Zhang, H.C.,Tang, Z.Y. Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: To investigate the preventive and therapeutic potential of garlic oil on human pancreatic carcinoma cells. Methods: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was performed to study the effects of garlic oil on three human pancreatic cancer cell lines, AsPC-1, Mia PaCa-2 and PANC-1. Cell cycle progression and apoptosis were detected by flow cytometry (FCM), staining with PI and annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI), respectively. Morphologic changes of pancreatic cancer cells were observed under transmission electron microscopy (TEM) after treatment with garlic oil at low inhibitory concentrations ($2.5{\mu}M$ and $10{\mu}M$) for 24 hours. Results: Proliferation of the AsPC-1, PANC-1, and Mia PaCa-2 cells was obviously inhibited in the first 24 hours with the MTT assay. The inhibition effect was more significant after 48 hours. When cells were exposed to garlic oil at higher concentrations, an early change of the apoptotic tendency was detected by FCM and TEM. Conclusion: Garlic oil could inhibit the proliferation of AsPC-1, PANC-1, and Mia PaCa-2 cells in this study. Moreover, due to programmed cell death, cell cycle arrest, or both, pro-apoptosis effects on AsPC-1 cells were induced by garlic oil in a dose and time dependent manner in vitro.

Explore the dynamic alternation of gene PLAC4 mRNA expression levels in maternal plasma in second trimester for nonivasive detection of trisomy 21

( Lan Yang ),( Hai Yan Sun ),( Dao Zhen Chen ),( Mu Dan Lu ),( Ye Tang ),( Jian Pin Xiao ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.4

Objective: Noninvasive prenatal detection of trisomy 21 (T21) has been achieved by measuring the ratio of two alleles of a single nucleotide polymorphism in circulating placenta specific 4 (PLAC4) mRNA in maternal plasma with a few assays in recent years. Our research is to explore the variations of PLAC4 mRNA expression level in maternal plasma with normal pregnancies in second trimester, which can provide pregnant women deeper insights with suitable detection period for the non-invasive prenatal detection of T21. Methods: We measured a serial plasma PLAC4 mRNA concentrations weekly from the same 25 singleton normal pregnant women. We recruited maternal plasma samples from 45 singleton pregnant women , comprising of 25 euploid pregnancies (control group; range, 17 to 21 weeks) and 20 T21 pregnancies (T21 group; range, 19 to 24 weeks). With the application of reverse transcription polymerase chain reaction, we achieved an insight of PLAC4 mRNA expression levels in maternal plasma during second trimester with euploid pregnancies. Results: Among the control group, the levels of PLAC4 mRNA expression in the gestation of 17 to 18 weeks were significantly less than those in the gestation of 18 to 21 weeks (P<0.05). The average PLAC4 mRNA concentration of the normal pregnant women was not higher than that of the T21 group (P>0.05). Conclusion: The PLAC4 mRNA showed a higher level of expression in the gestation of 18 to 21 weeks with an euploid pregnancy of pregnant women. We also found that there was no significant difference in plasma PLAC4 mRNA concentration between the normal and the T21 pregnancies in second trimester.

Syntheses, Structures and Luminescent Properties of Two Novel M(II)-Phen-SIP Supramolecular Compounds (M = Co, Ni)

Yu-Lan Zhu,Shuai Shao,Kui-Rong Ma,Xue-Ling Tang,Li Cao,Hui-Chao Zhao 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

Two metal compounds, [Co(phen)2(H2O)2]·2H2SIP·2H2O 1 and [Ni(phen)3]·2H2SIP·3H2O 2, have been obtained by incorporating 1,10-phenanthroline (phen) and 5-sulfoisophthalic acid monosodium salt (NaH2SIP) ligands under hydrothermal conditions. Meanwhile, the two compounds were characterized by element analysis, IR, XRD, TG-DTA and single-crystal X-ray diffraction. Both 1 and 2 present 3D supramolecular structures via O–H···O hydrogen bond interactions. Luminescent properties for 1 and 2 were also studied. The compound 1 has two fluorescence emission peaks centered at 398 nm attributed to the intraligand emission from the SIP ligand and at 438 nm assigned to the combined interaction of intraligand π*-π transitions of the phen ligand and ligand-to-metal-charge-transfer (LMCT) transitions (λex = 233 nm). The compound 2 shows one emission band centered at 423 nm with a shoulder peak at 434 nm which may be originated from the intraligand π*-π transitions of the phen ligand (λex = 266 nm).

Combined Study on Mold Taper and Corner Radius in Bloom Continuous Casting by FEM Simulation and Trial Experiment

Peng Lan,Liang Li,Zhanpeng Tie,Haiyan Tang,Jiaquan Zhang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.6

A plane stress model was developed to study the coupled effect of mold taper and corner radius on the thermal–mechanicalbehavior of a 250 mm × 280 mm continuously cast bloom for a given special steel. Good agreement was obtained in both shellthickness and the off-corner cracking location from modeling and trial experiment. The results show that increasing moldtaper results in the obvious decrease of surface temperature near the corner, while enlarging mold corner radius makes theshell surface temperature at the corner region more even with the mold taper between 0 and 1.5% m−1. It is also found thatboth the mold corner radius and taper are the key factors influencing the shell crack sensitivity but with different mechanism. For the internal crack, the mold taper takes more dominant effect for its corner radius between 0–10 mm, as the hoop strainat the solidification front decreases with increasing mold taper. For the surface crack, more sensitivity is noticed to the moldcorner radius. Increasing mold corner radius leads to the increase of the surface hoop strain in the corner region, almostregardless of mold taper. The proper mold taper and corner radius for the present bloom casting should be 1.0–1.5% m−1and 15–25 mm respectively.

Synthesis, Structures and Photoluminescent Properties of Two Novel Zinc(II) Compounds Constructed from 5-Sulfoisophthalic Acid

Yu-Lan Zhu,Xue-Ling Tang,Kui-Rong Ma,Hao Chen,Feng Ma,Lian-Hua Zhao 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.7

Hydrothermal reaction of zinc(II) salts with 5-sulfoisophthalic acid monosodium salt (NaO3SC6H3-1,3-(COOH)2, NaH2-SIP) and 1,10-phenanthroline (phen) led to two new compounds, [Zn(phen)3]·2H2SIP·4H2O (1) and [Zn(phen)2(H2O)2]·2H2SIP·2H2O (2). They were characterized by element analysis, IR spectroscopy, thermalgravimetric analysis (TGA),X-ray powder diffraction (XRD), and single-crystal X-ray diffraction. Both compounds 1-2 represent the first example of Zn/phen/SIP system. The Zn (II) ion in 1 is six-coordinated by six nitrogen atoms from three phen molecules, and the H2SIP‒ ligands engage in the formation of hydrogen bond. The Zn(II) ion in 2 is coordinated by four nitrogen atoms from two phen molecules and two oxygen atoms from two water molecules. Moreover, both 1 and 2 are assembled into 3D supramolecular architectures by hydrogen bonds (O-H···O) and π-π interactions. Solvent water molecules occupying voids of the compounds serve as receptors or donors of the extensive O-H···O hydrogen bonds.

Research Articles : Polymorphisms in BMP-2 gene and their associations with growth traits in goats

( Xing Tang Fang ),( Hai Xia Xu ),( Chun Lei Zhang ),( Jing Min Zhang ),( Xian Yong Lan ),( Chuan Wen Gu ),( Hong Chen ) 한국유전학회 2010 Genes & Genomics Vol.32 No.1

A Frame work of Automatic Analysis System of Electrocardiogram Signals

Xiao Tang,Shu Lan 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.2