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      • Automatic Sugar Beet Phenotyping in Open Field by a Computer Vision System

        ( Pieter M. Blok ),( Jochen Hemming ),( Youngki Hong ),( Jaesu Lee ),( Daehyun Lee ),( Gookhwan Kim ) 한국농업기계학회 2016 한국농업기계학회 학술발표논문집 Vol.21 No.2

        Crop growth is an important quality assessment in plant breeding, especially in open field crops which grow in fluctuating and unfavorable outdoor conditions. To evaluate the growth potential of different plant varieties, researchers conduct leaf area measurements of emerged plants to evaluate its growth potential. This is a time consuming and labor intensive activity and therefore often only conducted on random spots on the field. An automatic computer vision system was built to automate and to speed up this plant phenotyping process. The system consist of three color cameras mounted on an implement facing straight downwards, lamps for illumination, an encoder wheel and a computer system. Natural light was blocked by a surrounding cover to limit the effect of variable outdoor light conditions on the image quality. The computer vision software makes use of an excessive green algorithm (2G - R - B) to segment the plant material from the soil. As the crop plants are sown by a precision sowing device in a regular pattern a method based on the fast-fourier transform (FFT) is used to distinguish crop plants from weed plants. A rectangular based clustering algorithm, based on 8-pixel nearest-neighbor connectivity, is used to cluster separated plant-parts together as one individual plant object used to measure the leaf area. The system was validated in an open-field sugar beet crop at the growing stage off our leaves. Fifty-five sugar beet plants were manually measured by experienced plant scouts(“ground truth”). The same plants were measured with the computer vision system. An ANOVA F-test(P<0.05) was used to discriminate the two measurement methods. The F-probability was 0.055 an djust above the significance level. So the H0 hypothesis that there is not a difference between human measurement and machine vision measurement was no trejected. Possible causes of difference was the inability of the system to detect and measure plants damaged by animals and very small plants which were occluded by clods or bigger plants. Nevertheless,with improvements on the vision software and camera/lamp configuration, the system is profitable for a fast and accurate leaf area measurement and corresponding plant phenotyping.

      • SCISCIESCOPUS

        Numerical Modeling of in-Band Pumped Ho-Doped Silica Fiber Lasers

        Wang, Jiachen,Yeom, Dong-il,Simakov, Nikita,Hemming, Alexander,Carter, Adrian,Lee, Sang Bae,Lee, Kwanil IEEE 2018 Journal of Lightwave Technology Vol.36 No.24

        <P>A theoretical model to describe in-band pumped holmium doped silica fiber lasers is presented. The model is established based upon theory and parameters obtained from published research works. Both core-pumped system and cladding-pumped system are simulated with the model. The simulation results agree well with most experimental results, and for the cases in which the simulation results show discrepancy with the experimental results the disagreement can be reasonably explained. Through numerical analysis it is found that besides common negative factors such as non-radiative decay, fiber loss, and non-optimized resonator configuration, energy transfer upconversion plays a deleterious role in the performance of in-band pumped holmium doped silica fiber lasers. In particular, inhomogeneous upconversion associated with ion clustering is found to cause significant degradation of slope efficiencies of lasers, a fact that is useful to understand the unsolved problem regarding the lower-than-expected slope efficiencies observed in previous studies of in-band pumped holmium doped fiber lasers.</P>

      • KCI등재

        Sarus crane in lowlands of Nepal: Is it declining really?

        Hem Katuwal 국립중앙과학관 2016 Journal of Asia-Pacific Biodiversity Vol.9 No.3

        Sarus crane (Antigone antigone) is a flagship species. Its population is declining globally. First recorded in 1877 in Nepal, so far only a few studies have been conducted on sarus crane and results of these studies confirm their declining state. Based on previous studies, the author reviewed the status of sarus crane in Nepal. Studies show that it is uncommon with patchy distribution from Chitwan to Kanchanpur districts. More than 90% of its habitats lie outside the protected areas. Rupandehi and Kapilavastu are stronghold districts with more than 85% of its overall population. Regression analysis showed that the overall population of sarus crane has increased in Nepal. Hatching success is more than 50% and new breeding sites are also being reported. Nevertheless, threats such as drying of wetlands, conversion of farmlands to settlements and industries, power lines, nest vandalization still persist. Farmlands provide important foraging and breeding grounds. It inhibits and breeds very close to the human settlements. Thus, increasing awareness to local people and wetland/habitat restoration are necessary for its conservation. Detail scientific studies on its ecology and monitoring using cutting-edge technology in existing and new localities along with crane conservation action plan are required for maintaining the sarus crane population in Nepal.

      • SCOPUSKCI등재

        Letter to the Editor : A Case of Netherton Syndrome Responsive to 1% Topical Pimecrolimus Cream

        ( Hem In Lee ),( Jung U Shin ),( Kwang Hoon Lee ) 대한피부과학회 2014 대한피부과학회지 Vol.52 No.12

        Netherton syndrome is a rare autosomal recessive disorder associated with mutation of the SPINK5 gene. Its typical manifestations are the triad of ichthyosis, hair shaft abnormality, and atopic diathesis. We report a case of Netherton syndrome and suggest topical pimecrolimus as a well-tolerated agent for truncal lesions. A 17-year-old male patient presented with skin lesions involving the whole body and hair present shortly after his birth. On physical examination, there were polycyclic serpiginous patches with peripheral scales on the extremities (Fig. 1A, 1B). Erythematous lesions resembling atopic dermatitis were present on the popliteal fossa area; however, they were mild and without lichenification. Also, diffuse erythema was noted on the face with a positive Hertoghe sign and brittle hair (Fig. 1C). Clinically, the lesions with geographic, sharply defined erythema on the trunk were compatible with ichthyosis linearis circumflexa (ILC). Pathological findings revealed hyperkeratosis, acanthosis, and parakeratosis along with perivascular cellular infiltration, mostly lymphocytic, in the upper dermis (Fig. 2A). Also, a closer inspection of the patient’s hair with light microscopy and scanning electromicrography yielded findings suspicious for trichorrhexis invaginata (Fig. 2B). Complete blood count and routine blood chemistry were all within normal limits; however, total IgE was elevated (3,449 IU/mL) and allergen-specific IgE to Dermatophagoides farinae was strongly positive (class 6) on ImmunoCAP®. The combination of ichthyosis, hair shaft abnormality, and atopic tendency satisfied the diagnostic triad of Netherton syndrome. For treatment, 1% topical pimecrolimus ointment was applied once daily for 6 months. No adverse events occurred, and the most prominent changes occurred over the extremities with a reduction of scaly hyperkeratotic plaques (Fig. 1D, 1E). However, the facial erythema persisted despite the applica tion of pimecrolimus. There was an approximately >50% reduction of the Netherton Area and Severity Assessment score from the initial value. There is no set guideline for the treatment of Netherton syndrome. Topical steroid, topical pimecrolimus, tacrolimus, calcipotriene, 12% ammonium lactate, systemic retinoid, and NB-UVB are some of the options available. Among these treatments, pimecrolimus has been suggested as an effective choice for ILC1. Treatment with pimecrolimus for 18 months caused a dramatic reduction in disease severity and pruritus score compared with baseline2. Also, despite concerns of decreased protease inhibition within the epidermis and defective barrier function increasing the risk of transepidermal water loss, skin infection, and, most important, systemic absorption of topical drugs3, treatment of 99% of the body surface area with pimecrolimus did not result in its increased level in blood, assuring its relative safety4. The atopic dermatitis on flexural sites and ichthyosis on the trunk and extremities in our patient showed improvement after treatment with pimecrolimus. However, no effect on facial erythema was seen. Diffuse erythema on the face is commonly noted in Netherton syndrome. Yet, it is still unclear whether the major contributory factors for persistent erythema are a result of an ineffective barrier, vasculature abnormality, or environmental effects. We report an educational case reminding of the classical triad of Netherton syndrome and showing the effectiveness of topical pimecrolimus. Our patient showed comparable results to those reported in Western countries; however, we additionally suggest the need for future studies aimed at uncovering the mechanism of facial erythema refractory to treatment.

      • KCI등재

        Residual Analysis of Insecticides (Lambda-cyhalothrin, Lufenuron, Thiamethoxam and Clothianidin) in Pomegranate Using GC-μECD or HPLC-UVD

        Hem, Lina,Park, Jong-Hyouk,Shim, Jae-Han The Korean Society of Environmental Agriculture 2010 한국환경농학회지 Vol.29 No.3

        In this study, the residual levels of four insecticidal compounds (lambda-cyhalothrin, lufenuron, thiamethoxam, and clothianidin) were monitored in the pomegranate, in order to assess the risk to consumers posed by the presence of such residues. The insecticides were applied at the recommended dose rates onto pomegranate trees. The samples were then collected at harvesting time after several treatments (two, three, and four treatments). After sample preparation progressed through the clean-up procedure, lufenuron, thiamethoxam, and clothianidin residues were analyzed via a HPCL-UVD, and the lambda-cyhalothrin residue was analyzed via a GC-${\mu}ECD$. The versatility of this method was evidenced by its excellent linearity (>0.9998 to 1) at broad concentration ranges. The mean recoveries evaluated from the untreated sample spiked with two different fortification levels ranged from 72.45 to 113.90%, and the repeatability (as a relative standard deviation) resulted from triplicate recovery tests was in a range from 0.80 to 11.75%. The residues of all insecticides determined from treated pomegranate samples and their LOD levels (lunfenuron, 0.01; lambda-cyhalothrin, 0.005; thiamethoxam, 0.01; clothianidin, 0.02 mg/kg) were much lower than their MRLs (0.5 mg/kg).

      • SCISCIESCOPUSKCI등재
      • SCOPUSKCI등재

        Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS

        Hem, Lina,Khay, Sathya,Choi, Jeong-Heui,Morgan, E.D.,El-Aty, A.M. Abd,Shim, Jae-Han Korean Society of ToxicologyKorea Environmental Mu 2010 Toxicological Research Vol.27 No.2

        The pesticide trichlorfon is readily degraded under experimental conditions to dichlorvos. A method has therefore been developed by which residues of trichlorfon in milk are determined as dichlorvos, using gas chromatography with ${\mu}$-electron capture detection. The identification of dichlorvos was confirmed by mass spectrometry. Milk was extracted with acetonitrile followed by centrifugation, freezing lipid filtration, and partitioning into dichloromethane. The residue after partitioning of dichloromethane was dissolved in ethyl acetate for gas chromatography. Recovery concentration was determined at 0.5, 1.0, and 2.0 of times the maximum permitted residue limits (MRLs) for trichlorfon in milk. The average recoveries (n = 6) ranged from 92.4 to 103.6%. The repeatability of the measurements was expressed as relative standard deviations (RSDs) ranging from 3.6%, to 6.7%. Limit of detection (LOD) and limit of quantification (LOQ) were 3.7 and $11.1{\mu}g/l$, respectively. The accuracy and precision (expressed as RSD) were estimated at concentrations from 25 to $250{\mu}g/l$. The intra- and inter-day accuracy (n = 6) ranged from 89.2% to 91% and 91.3% to 96.3%, respectively. The intra- and inter-day precisions were lower than 8%. The developed method was applied to determine trichlorfon in real samples collected from the seven major cities in the Republic of Korea. No residual trichlorfon was detected in any samples.

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