Fabrication of a standalone MEA shell using silver nanowire

Nguyen Trung Hai(Hai Trung Nguyen),Ki Ju Kang(강기주) 대한기계학회 2019 대한기계학회 춘추학술대회 Vol.2019 No.4

Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function

Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

Development of label-free electrochemical lactose biosensor based on graphene/poly(1,5-diaminonaphthalene) film

Binh Hai Nguyen,Binh Thanh Nguyen,Hanh Van Vu,Chuc Van Nguyen,Dzung Tuan Nguyen,Loc Thai Nguyen,Thu Thi Vu,Lam Dai Tran 한국물리학회 2016 Current Applied Physics Vol.16 No.2

In this work, a lactose biosensor was developed by co-immobilizing b-galactosidase (b-Gal) and glucose oxidase (GOx) on microelectrodes pre-modified with Pt/graphene/P(1,5-DAN) for estimation of lactose in dairy products to prevent lactose intolerance. The Pt microelectrode was modified with graphene and 1,5-polydiaminonaphthalene film. Graphene was synthesized by chemical vapor deposition on copper tape and manually transferred to the electrode surface. Polymeric P(1,5-DAN) was grafted on top of the graphene film by electropolymerization. Modified surface of the electrode was characterized by Raman spectra analysis, FE-SEM, AFM and cyclic voltammetry. The results indicated that deposition graphene film on electrode surface induced considerable enhancement in current signal, over 20 times as high as the uncoated electrode surface. The developed sensor was successfully used to determine lactose in model samples with sensitivity, correlation coefficient (R2) and limit of detection (LOD) estimated to be 1.33 mA/(mgml1), 0.995 and 1.3 mg/ml, respectively. The combined graphene and conductive P(1,5-DAN) could serve as a novel sensing platform on electrochemical sensors with superior sensitivity.

The extraction of lignocelluloses and silica from rice husk using a single biorefinery process and their characteristics

Ngoc Thuy Nguyen,Nhat Thong Tran,Tan Phat Phan,Anh Thu Nguyen,My Xuyen T. Nguyen,Nguyen Ngan Nguyen,Young Ho Ko,Dai Hai Nguyen,Tran T.T. Van,DongQuy Hoang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-

While the efficient usage of biomass waste can significantly help in addressing environmental issues,there are only a few reports that discuss about processing such waste effectively at a low-cost. Such challengearises from the strong association between the components biomass. In this study, an abundantagricultural byproduct, rice husk (RH), was used as the starting resource. A simple biorefining processof alkaline peroxide treatment followed by acid precipitation and ethanol extraction was performed onRH to obtain cellulose, hemicellulose, lignin, and silica. The chemical structures, morphologies, andphysic-chemical properties of the separated components were identified through a wide range of characterizationapproaches. The final products obtained from of this process were (i) bundles of fiber-likecellulose with a fiber width of 6 mm and (ii) small particles of hemicellulose and lignin with nonuniformshapes. The lignocelluloses products had over 90 wt% carbon with 52.28% crystalline ratio. Meanwhile, the other products comprising hemicelluloses, lignin, and silica were amorphous. The outcomeof this study contributes to expanding and developing the simple and efficient conversion processof biomass waste into sustainable value-added materials. It is crucial to reduce the environmental impactby using renewable materials as the new building block resources for synthetic chemicals.

Parenchymal-sparing anatomical hepatectomy based on portal ramification of the right anterior section: A prospective multicenter experience with short-term outcomes

Truong Giang Nguyen,Thanh Khiem Nguyen,Ham Hoi Nguyen,Hong Son Trinh,Tuan Hiep Luong,Minh Trong Nguyen,Van Duy Le,Hai Dang Do,Kieu Hung Nguyen,Van Minh Do,Quang Huy Tran,Cuong Thinh Nguyen 한국간담췌외과학회 2024 Annals of hepato-biliary-pancreatic surgery Vol.28 No.1

Backgrounds/Aims: Parenchymal-sparing anatomical hepatectomy (Ps–AH) based on portal ramification of the right anterior section (RAS) is a new technique to avoid unnecessarily transecting too much liver parenchyma, especially in cases of major anatomical hepatectomy. Methods: We prospectively assessed 26 patients with primary hepatic malignancies having undergone major Ps–AH based on portal ramification of the RAS from August 2018 to August 2022 (48 months). The perioperative indications, clinical data, intra-operative index, pathological postoperative specimens, postoperative complications, and follow-up results were retrospectively evaluated. Results: Among the 26 patients analyzed, there was just one case that had intrahepatic cholangiocarcinoma The preoperative level of α– Fetoprotein was 25.2 ng/mL. All cases (100%) had Child–Pugh A liver function preoperatively. The ventral/dorsal RAS was preserved in 19 and 7 patients, respectively. The mean surgical margin was 6.2 mm. The mean surgical time was 228.5 minutes, while the mean blood loss was 255 mL. In pathology, 5 cases (19.2%) had microvascular invasion, and in the group of HCC patients, 92% of all cases had moderate or poor tumor differentiation. Six cases (23.1%) of postoperative complications were graded over III according to the Clavien–Dindo system, including in three patients resistant ascites or intra-abdominal abscess that required intervention. Conclusions: Parenchymal-sparing anatomical hepatectomy based on portal ramification of the RAS to achieve R0-resection was safe and effective, with favorable short-term outcomes. This technique can be used widely in clinical practice.

Two New C-Glucosyl Benzoic Acids and Flavonoids from Mallotus nanus and Their Antioxidant Activity

Phan Van Kiem,Nguyen Thi Mai,Chau Van Minh,Nguyen Huu Khoi,Nguyen Hai Dang,Nguyen Phuong Thao,Nguyen Xuan Cuong,Nguyen Hoai Nam,Nguyen Xuan Nhiem,Yvan Vander Heyden,Joëlle Quetin-Leclercq,김교남,장해동,김영호 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.2

Two new 2-C-β-D-glucopyranosyl benzoic acid derivatives named mallonanosides A (1) and B (2) were isolated from the methanolic extract of the leaves of Mallotus nanus along with five known flavonoids, kaempferin (3), juglanin (4), quercitrin (5), myricitrin (6), and rhoifolin (7). Their structures were established on the basis of spectral and chemical evidence. Their antioxidant activities were shown to depend on the number of hydroxyl groups, and the location and species of sugar moiety.

Large Magnetocaloric Effect above 300 K and Magnetoresistance in (La0.5Pr0.5)1-xPbxMnO3 Perovskites

Nguyen Chau,Duong Thi Hanh,Bui Cong Tinh,Nguyen Hoang Luong,Nguyen Duc Tho,Nguyen Hoang Hai 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

Perovskite compounds (La0.5Pr0.5)₁- xPbxMnO₃ (x = 0.1 - 0.5) with orthorhombic structure were prepared by using a solid-state reaction technique. In the studied samples, the spin-glass-like state occurs at low temperatures and the Curie temperature, Tc, increases from 170 K (x = 0.1) to 336 K (x = 0.5) because of a strong double exchange. The magnetic entropy change, │△Sm│, reached its largest value of 2.06 J/kg·K at △H = 13.5 kOe for the sample with x = 0.4 (Tc = 323 K). Due to the large │△Sm│ and high Tc, these materials are suggested for use as active magnetic refrigerants for magnetic refrigeration technology at temperatures above room temperature. While the conductivity of the samples with x = 0.1 and 0.2 exhibits only a semiconducting behavior over the whole measured temperature range, there is insulator-metallic phase transition on the R(T) curves of the samples with x = 0.3 - 0.5. The magnetoresistance of the samples was measured and large values for MR were found. Perovskite compounds (La0.5Pr0.5)₁- xPbxMnO₃ (x = 0.1 - 0.5) with orthorhombic structure were prepared by using a solid-state reaction technique. In the studied samples, the spin-glass-like state occurs at low temperatures and the Curie temperature, Tc, increases from 170 K (x = 0.1) to 336 K (x = 0.5) because of a strong double exchange. The magnetic entropy change, │△Sm│, reached its largest value of 2.06 J/kg·K at △H = 13.5 kOe for the sample with x = 0.4 (Tc = 323 K). Due to the large │△Sm│ and high Tc, these materials are suggested for use as active magnetic refrigerants for magnetic refrigeration technology at temperatures above room temperature. While the conductivity of the samples with x = 0.1 and 0.2 exhibits only a semiconducting behavior over the whole measured temperature range, there is insulator-metallic phase transition on the R(T) curves of the samples with x = 0.3 - 0.5. The magnetoresistance of the samples was measured and large values for MR were found.

Composites derived from synthetic clay and carbon sphere: Preparation, characterization, and application for dye decontamination

Nguyen Duy Dat,Ton That Loc,Mai Thuan Trieu,Dong Thanh Nguyen,Khuong Quoc Nguyen,My Linh Nguyen,Anh Duy Duong Le,Hai Nguyen Tran 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.4

Two new composites from synthetic clay-like materials and carbon spheres were developed. Layered doubledhydroxides (LDH) were synthesized from the coprecipitation of Mg2+ and Al3+ ions. Spherical hydrochar (SH) wasprepared from pure glucose through hydrothermal carbonization at 190 oC. The composite LDH–SH was synthesizedthrough a simple hydrothermal method of the mixture of LDH and SH. Another composite, LDO-SB, was directly preparedthrough the carbonization of LDH-SH at 500 oC. Under such high temperature, LDH was converted to layereddoubled oxides (LDO), and SH was transferred to spherical biochar (SB). Those materials were characterized by chemicalstability, surface morphology and element composition, crystal structure, surface functional group, and texturalcharacteristic. They were applied for removing cationic dye (methylene blue; MB) and anionic dye (Congo red; CR)under different pH solutions. Three adsorption components—kinetics, isotherm, and thermodynamics—were conductedunder batch experimenters. Results demonstrated that the LDH or LDO particles were assembled on the surfaceof SH or SB, respectively. The surface area, total pore volume, and average pore width of LDH–SH and LDO-SBwere 58.5 and 198m2/g, 0.319 and 0.440 cm3/g, and 21.8 and 8.89 nm, respectively. The maximum adsorption capacityof the materials, calculated from the Langmuir model, at 30 oC for CR and MB dyes was 1589 and 78.6mg/g (LDOSB)and 499 and 226mg/g (LDH-SH), respectively. The composites exhibited a higher affinity to anionic than cationicdyes, which resulted from the great contribution of the clay-like materials. Therefore, they can serve as a promisingcomposite for the decolorization of wastewater.

A Novel Robotic Platform for Aerial Manipulation Using Quadrotors as Rotating Thrust Generators

Nguyen, Hai-Nguyen,Park, Sangyul,Park, Junyoung,Lee, Dongjun IEEE 2018 IEEE transactions on robotics Vol.34 No.2

<P>We propose a novel robotic platform for aerial operation and manipulation, a spherically connected multiquadrotor (SmQ) platform, which consists of a rigid frame and multiple quadrotors that are connected to the frame via passive spherical joints and act as distributed rotating thrust generators to collectively propel the frame by adjusting their attitude and thrust force. Depending on the number of quadrotors and their configuration, this SmQ platform can fully (or partially) overcome the issues of underactuation of the standard multirotor drones for aerial operation/manipulation (e.g., body-tilting with sideway gust/force, dynamic interaction hard to attain, complicated arm–drone integration, etc.). We present the dynamics modeling of this SmQ platform system and establish the condition for its full actuation in SE(3). We also show how to address limited range of spherical joints and rotor saturations as a constrained optimization problem by noticing the similarity with the multifingered grasping problem under the friction-cone constraint. We then design and analyze feedback control laws for the S3Q and S2Q systems as a combination of high-level Lyapunov control design and low-level constrained optimization and show that the (fully actuated) S3Q system can assume any trajectory in SE(3), whereas the S2Q system in <TEX>$\Re^3\, \times$</TEX> S<TEX>$^2$</TEX> with its unactuated dynamics is still internally stable. Experiments are also performed to show the efficacy of the theory.</P>

Sulfate Reduction for Bioremediation of AMD Facilitated by an Indigenous Acidand Metal-Tolerant Sulfate-Reducer

( Hai Thi Nguyen ),( Huong Lan Nguyen ),( Minh Hong Nguyen ),( Thao Kim Nu Nguyen ),( Hang Thuy Dinh ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Acid mine drainage (AMD) has been a serious environmental issue that threatens soil and aquatic ecosystems. In this study, an acid-tolerant sulfate-reducing bacterium, strain S4, was isolated from the mud of an AMD storage pond in Vietnam via enrichment in anoxic mineral medium at pH 5. Comparative analyses of sequences of the 16S rRNA gene and dsrB gene involved in sulfate reduction revealed that the isolate belonged to the genus Desulfovibrio, and is most closely related to Desulfovibrio oxamicus (with 99% homology in 16S rDNA sequence and 98% homology in dsrB gene sequence). Denaturing gradient gel electrophoresis (DGGE) analyses of dsrB gene showed that strain S4 represented one of the two most abundant groups developed in the enrichment culture. Notably, strain S4 was capable of reducing sulfate in low pH environments (from 2 and above), and resistance to extremely high concentration of heavy metals (Fe 3,000 mg/l, Zn 100 mg/l, Cu 100 mg/ l). In a batch incubation experiment in synthetic AMD with pH 3.5, strain S4 showed strong effects in facilitating growth of a neutrophilic, metal sensitive Desulfovibrio sp. strain SR4H, which was not capable of growing alone in such an environment. Thus, it is postulated that under extreme conditions such as an AMD environment, acid- and metal-tolerant sulfate-reducing bacteria (SRB)- like strain S4 would facilitate the growth of other widely distributed SRB by starting to reduce sulfate at low pH, thus increasing pH and lowering the metal concentration in the environment. Owing to such unique physiological characteristics, strain S4 shows great potential for application in sustainable remediation of AMD.