Inductively Coupled Plasma Etching of AZO and ZnO in CI2/CH4/H2/Ar and BCI₃/CH4/H2/Ar Chemistries

Hack Joo Lee,Bong Soo Kwon,Young Rok Park,Joung Ho Ahn,Jin Sung Kim,Sun Il Kim,Hyun Woo Kim,Nae Eung Lee 한국표면공학회 2008 한국표면공학회 학술발표회 초록집 Vol.2008 No.-

Mid-term Clinical Outcomes in a Cohort of Asymptomatic or Mildly Symptomatic Korean Patients with Bicuspid Aortic Valve in a Tertiary Referral Hospital

Byung Joo Sun,Jin Kyung Oh,Sun Hack Lee,Jeong Yoon Jang,Ji Hye Lee,Sahmin Lee,Dae-Hee Kim,JongMinSong,Duk-Hyun Kang,Jae-Kwan Song 한국심초음파학회 2019 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.27 No.2

BACKGROUND: Although bicuspid aortic valve (BAV) is one of the most common congenital heart diseases, clinical outcome data regarding BAV are still limited. We evaluated clinical characteristics and mid-term clinical outcomes of asymptomatic Korean patients with bicuspid aortic valve. METHODS: We initiated a prospective registry in 2014 at a tertiary referral hospital. To develop a cohort of asymptomatic patients, we excluded patients who previously underwent open heart surgery (OHS) or who had OHS within 6 months of referral. RESULTS: A total of 170 patients (117 male [69%], age 50 ± 13 years) were enrolled. More than 70% (n = 124, 73%) were incidentally diagnosed with BAV during routine health examinations or preoperative screening for non-cardiac surgery. At the time of enrollment, moderate to severe aortic stenosis (AS) or regurgitation (AR) was present in 77 patients (45%) and 98 (58%) showed aortic dilation: 42 (25%) had non-significant valvular dysfunction without aortic dilation. During a median follow-up of 4 years, AS severity increased significantly (p < 0.001), while there was no significant change in AR severity (p = 0.361). A total of 27 patients (16%) underwent OHS, including isolated aortic valve (AV) surgery (n = 11, 41%), AV with combined aortic surgery (n = 12, 44%), and isolated aortic surgery (n = 4, 15%): no patient developed aortic dissection. Moderate to severe AS (hazard ratio [HR] 4.61; 95% confidence interval [CI] 1.83-11.62; p = 0.001), NYHA class ≥ 2 (HR 2.53; 95% CI 1.01-6.35; p = 0.048) and aortic dilation (HR 2.13; 95% CI 0.87-5.21; p = 0.099) were associated with surgical events. CONCLUSIONS: Progression patterns of valvular dysfunction and impacts of BAV phenotype on OHS should be explored in future studies with longer follow-up durations.

단신 : 페달링 시 정량적인 동적 피팅을 위한 실시간 평가 시스템

이주학 ( Joo Hack Lee ),강동원 ( Dong Won Kang ),배재혁 ( Jae Hyuk Bae ),신윤호 ( Yoon Ho Shin ),최진승 ( Jin Seung Choi ),탁계래 ( Gye Rae Tack ) 한국운동역학회 2014 한국운동역학회지 Vol.24 No.2

In this study, a real-time evaluation system for quantitative dynamic fitting during pedaling was developed. The system is consisted of LED markers, a digital camera connected to a computer and a marker detecting program. LED markers are attached to hip, knee, ankle joint and fifth metatarsal in the sagittal plane. Playstation3 eye which is selected as a main digital camera in this paper has many merits for using motion capture, such as high FPS (Frame per second) about 180FPS, 320×240 resolution, and low-cost with easy to use. The maker detecting program was made by using Labview2010 with Vision builder. The program was made up of three parts, image acquisition & processing, marker detection & joint angle calculation, and output section. The digital camera`s image was acquired in 95FPS, and the program was set-up to measure the lower-joint angle in real-time, providing the user as a graph, and allowing to save it as a test file. The system was verified by pedalling at three saddle heights (knee angle: 25, 35, 45o) and three cadences (30, 60, 90 rpm) at each saddle heights by using Holmes method, a method of measuring lower limbs angle, to determine the saddle height. The result has shown low average error and strong correlation of the system, respectively, 1.18±0.44o, 0.99±0.01o. There was little error due to the changes in the saddle height but absolute error occurred by cadence. Considering the average error is approximately 1°, it is a suitable system for quantitative dynamic fitting evaluation. It is necessary to decrease error by using two digital camera with frontal and sagittal plane in future study.

The outcome of hematopoietic stem cell transplantation in Korean children with hemophagocytic lymphohistiocytosis

Yoon, Hoi Soo,Im, Ho Joon,Moon, Hyung Nam,Lee, Jae Hee,Kim, Hee-Jin,Yoo, Keon Hee,Sung, Ki Woong,Koo, Hong Hoe,Kang, Hyung Jin,Shin, Hee Young,Ahn, Hyo Seop,Cho, Bin,Kim, Hack Ki,Lyu, Chuhl Joo,Lee, M Blackwell Publishing Ltd 2010 Pediatric transplantation Vol.14 No.6

<P>Yoon HS, Im HJ, Moon HN, Lee JH, Kim H-J, Yoo KH, Sung KW, Koo HH, Kang HJ, Shin HY, Ahn HS, Cho B, Kim HK, Lyu CJ, Lee MJ, Kook H, Hwang TJ, Seo JJ. The outcome of hematopoietic stem cell transplantation in Korean children with hemophagocytic lymphohistiocytosis.Pediatr Transplantation 2010: 14:735–740. © 2010 John Wiley & Sons A/S.</P><P>Abstract: </P><P>Chemoimmunotherapy-based treatments have improved the survival of patients with HLH, but outcomes of the patients are still unsatisfactory. We report here the outcome of Korean children with HLH who underwent HSCT, which was analyzed from the data of a nation-wide HLH registry. Retrospective nation-wide data recruitment for the pediatric HLH patients diagnosed between 1996 and 2008 was carried out by the Histiocytosis Working Party of the Korean Society of Hematology. Nineteen patients who received HSCT among the total of 148 enrolled children with HLH were analyzed for the transplant-related variables and events. The probability of five-yr survival after HSCT was 73.3% with a median follow-up of 57. Two months compared to 54.3% for the patients who were treated with chemoimmunotherapy only (p<I> </I>=<I> </I>0.05). The reasons for HSCT were active disease after eight wk of initial treatment (n = 9), relapsed disease (n = 5), and FHL (n = 5). Fourteen patients are currently alive without disease after HSCT, four patients died of treatment-related events (infection in two and graft failure in two) at early post-transplant period, and one patient died of relapse at one yr post transplantation. The survival of patients who were transplanted because of active disease after eight wk of initial treatment was worse compared to those patients who had inactive state at that time (60.6% vs. 100%, respectively, p<I> </I>=<I> </I>0.06). Of the four patients who received transplants using cord blood, three died of graft failure (n = 2) and relapse (n = 1). The five-yr probability of survival after HSCT according to the donor type was 85.7% for the MRDs (n = 6), 87.5% for the MUDs (n = 8), and 40% for the MMUDs (n = 5) (p<I> </I>=<I> </I>0.03). Other variables such as age, CNS involvement at the time of diagnosis, the etiology of HLH (familial or secondary), and the conditioning regimens had no influence on the five-yr OS of the HLH patients who underwent HSCT. HSCT improved the survival of the patients who had familial, relapsed, or severe and persistent SHLH in the Korean nation-wide HLH registry. Although numbers were small, these results are similar to other reports in the literature. The disease state after initial treatment, the stem cell source of the transplant, and the donor type were the important prognostic factors that affected the OS of the HLH patients who underwent HSCT.</P>

Resistant hypertension: consensus document from the Korean society of hypertension

Park Sungha,Shin Jinho,임상현,김광일,Kim Hack-Lyoung,Kim Hyeon Chang,Lee Eun Mi,Lee Jang Hoon,Ahn Shin Young,Cho Eun Joo,Kim Ju Han,Kang Hee-Taik,Lee Hae-Young,Lee Sunki,Kim Woohyeun,Park Jong-Moo 대한고혈압학회 2023 Clinical Hypertension Vol.29 No.-

Although reports vary, the prevalence of true resistant hypertension and apparent treatment-resistant hypertension (aTRH) has been reported to be 10.3% and 14.7%, respectively. As there is a rapid increase in the prevalence of obesity, chronic kidney disease, and diabetes mellitus, factors that are associated with resistant hypertension, the prevalence of resistant hypertension is expected to rise as well. Frequently, patients with aTRH have pseudoresistant hypertension [aTRH due to white-coat uncontrolled hypertension (WUCH), drug underdosing, poor adherence, and inaccurate office blood pressure (BP) measurements]. As the prevalence of WUCH is high among patients with aTRH, the use of out-of-office BP measurements, both ambulatory blood pressure monitoring (ABPM) and home blood pressure monitoring (HBPM), is essential to exclude WUCH. Non-adherence is especially problematic, and methods to assess adherence remain limited and often not clinically feasible. Therefore, the use of HBPM and higher utilization of singlepill fixed-dose combination treatments should be emphasized to improve drug adherence. In addition, primary aldosteronism and symptomatic obstructive sleep apnea are quite common in patients with hypertension and more so in patients with resistant hypertension. Screening for these diseases is essential, as the treatment of these secondary causes may help control BP in patients who are otherwise difficult to treat. Finally, a proper drug regimen combined with lifestyle modifications is essential to control BP in these patients.

Quantitation of Plasma Apolipoprotein A-I with a Sandwich Type Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies

Lee, Min-Gyu,Kang, Jae-Seon,Jeong, Jae-Yeon,Jue, Dae-Myung,Kim, Hack-Joo Korean Society for Biochemistry and Molecular Biol 1997 Journal of biochemistry and molecular biology Vol.30 No.6

A sandwich-type enzyme-linked immunosorbent assay (ELISA) for the quantification of human apolipoprotein A-I (apoA-I) was developed using monoclonal antibodies. For this assay, we used three monoclonal antibodies to trap and detect apo A-I. HDAI16 and HDA15 monoclonal antibodies were used for trapping apoA-I and HDAI8 monoclonal antibody was for detecting apoA-I. These three monoclonal antibodies were produced by immunizing mice with high density lipoprotein (HDL) isolated from human plasma. By immunoblot analysis, these three monoclonal antibodies were specific to apoA-I and showed no cross-reactivities with other plasma proteins. The results of competition assays for epitope cross-reactivity test also verified that these monoclonal antibodies identified separate and distinct epitopes on HDL and apoA-I. Affinity constants of monoclonal antibodies were measured by ELISA. Their association constants ranged from $10^7$ to $10^8$ $M^{-1}$. For this assay, pure apoA-I was isolated by affinity chromatography using monoclonal antibodies. In this sandwich assay, the amount of HRP-labeled HDAI8 bound to apoA-I trapped by HDAI16 and HDAI5 was proportional to apoA-I concentration in the range of 0 to 500ng/ml. ApoA-I concentration in plasma was calculated from the linear regression equation of standard curve. The precision and reliability of the assays are reflected in the low intra-and interassay coefficients of variation that averaged 3.25% and 4.30%, respectively. This assay is sensitive, simple, reproducible, convenient in incubation interval, and does not use radioisotope: thus it can be widely applied in clinical laboratories.

Findings of Single-Photon Emission Computed Tomography and Its Relation with Quantitative Coronary Angiography in Patients with Significant Stenosis of the Left Main Coronary Artery

Hack-Lyoung Kim,So Won Oh,Hyunjong Lee,Hee Jun Kim,You Nui Kim,Woo-Hyun Lim,Jae-Bin Seo,Sang-Hyun Kim,Myung-A Kim,Joo-Hee Zo 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.1

Objective: Unrecognized left main coronary artery disease (LMCD) is often fatal; however, accuracy of non-invasive tests for diagnosing LMCD is still unsatisfactory. This study was performed to elucidate single-photon emission computed tomography (SPECT) detection of LMCD using quantitative coronary angiography (QCA) data. Materials and Methods: Fifty-five patients (39 men; mean age, 68.1 ± 10.9 years) diagnosed with significant left main (LM) stenosis (≥ 50%) by invasive coronary angiography (ICA) were retrospectively reviewed. All study patients underwent SPECT with pharmacologic stress within 30 days of ICA. All coronary lesions were quantified via QCA, and SPECT findings were compared with QCA results. Results: Only four patients (7.3%) had isolated LMCD; all others had combined significant stenosis (≥ 70%) of one or more other epicardial coronary arteries. Patients with more severe coronary artery disease tended to have higher values for summed difference scores in a greater number of regions, but the specific pattern was not clearly defined. Summed stress score of SPECT did not differ according to LM stenosis severity. Only three patients (5.4%) had a typical LM pattern of reversible perfusion defect on SPECT. A significant negative linear correlation between stenosis severity and stress perfusion percent was found in the left anterior descending artery region (r = -0.455, p < 0.001) but not in the left circumflex artery. Conclusion: Single-photon emission computed tomography findings were heterogeneous, not specific and poorly correlated to QCA data in patients with significant LMCD. This may be due to highly prevalent significant stenosis of other epicardial coronary arteries.

Flavonoids from <i>Machilus japonica</i> Stems and Their Inhibitory Effects on LDL Oxidation

Joo, Se-Jin,Park, Hee-Jung,Park, Ji-Hae,Cho, Jin-Gyeong,Kang, Ji-Hyun,Jeong, Tae-Sook,Kang, Hee Cheol,Lee, Dae-Young,Kim, Hack-Soo,Byun, Sang-Yo,Baek, Nam-In MDPI 2014 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.15 No.9

<P>Stems of <I>Machilus japonica</I> were extracted with 80% aqueous methanol (MeOH) and the concentrated extract was successively extracted with ethyl acetate (EtOAc), normal butanol (<I>n</I>-BuOH), and water. Six flavonoids were isolated from the EtOAc fraction: (+)-taxifolin, afzelin, (−)-epicatechin, 5,3'-di-<I>O</I>-methyl-(−)-epicatechin, 5,7,3'-tri-<I>O</I>-methyl-(−)-epicatechin, and 5,7-di-<I>O</I>-methyl-3',4'-methylenedioxyflavan-3-ol. The chemical structures were identified using spectroscopic data including NMR, mass spectrometry and infrared spectroscopy. This is the first report of isolation of these six compounds from <I>M. japonica</I>. The compounds were evaluated for their diphenyl picryl hydrazinyl scavenging activity and inhibitory effects on low-density lipoprotein oxidation. Compounds <B>1</B> and <B>3</B>–<B>6</B> exhibited DPPH antioxidant activity equivalent with that of ascorbic acid, with half maximal inhibitory concentration (IC<SUB>50</SUB>) values of 0.16, 0.21, 0.17, 0.15 and 0.07 mM, respectively. The activity of compound <B>1</B> was similar to the positive control butylated hydroxytoluene, which had an IC<SUB>50</SUB> value of 1.9 µM, while compounds <B>3</B> and <B>5</B> showed little activity. Compounds <B>1</B>, <B>3</B>, and <B>5</B> exhibited LDL antioxidant activity with IC<SUB>50</SUB> values of 2.8, 7.1, and 4.6 µM, respectively.</P>

Total synthesis of (−)-kaitocephalin

Lee, Wonchul,Youn, Joo-Hack,Kang, Sung Ho The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.45

<P>(−)-Kaitocephalin has been synthesized. With the C9 stereocenter from Garner's aldehyde, the C4 quaternary carbon was installed by the desymmetrization of the Cbz-protected serinol. The remaining stereogenic centers were generated through mercuriocyclization, epoxidation and regioselective epoxide opening, in which the quaternary carbon most likely played crucial roles in the stereoinduction.</P> <P>Graphic Abstract</P><P>Total synthesis of (−)-kaitocephalin has been completed through desymmetrization for the quaternary carbon, mercuriocyclization for the pyrrolidine, epoxidation followed by epoxide opening for the amino hydroxy functionality and concurrent oxidation for the three carboxylic acids. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc42365d'> </P>

소아기의 만성 골수성 단핵구성 백혈병 1례

이채화,주신행,김학기,이경수,심상인 최신의학사 1987 最新醫學 Vol.30 No.9