Graphene Oxide : Bioglogical Reponses and Applications

Guan-Chen LIN,Hsing-Yu TUAN,Guan-Yu CHEN,Yu-Chen HU 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.4

Enhanced photocatalytic activity of TiO2/graphene by tailoring oxidation degrees of graphene oxide for gaseous mercury removal

Yu Guan,Tao Hu,Jiang Wu,Lili Zhao,Fengguo Tian,Wei-Guo Pan,Ping He,Xuemei Qi,Fangqin Li,Kai Xu 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.1

We used a simple method of graphene oxide (GO) preparation with different oxidation levels, and control the properties of the TiO2 nanocrystals by tuning the content and oxidation degree of GO to enhance the photocatalytic performance. During the hydrothermal reaction, reduction of GO, formation of TiO2 and chemical bonds between TiO2 and reduced graphene oxide (RGO) was achieved simultaneously. Characterization results showed that TiO2 properties such as crystalline grain and particle size could be tailored by the amount of functional groups, and that crystallinity was also controlled by GO degrees of oxidation. TiO2/RGO photocatalysts showed great efficiency of mercury oxidation, which reached 83.7% and 43.6% under UV and LED light irradiation, respectively. The effects of crystalline grain size and surface chemical properties on Hg0 removal under LED and UV light irradiation were analyzed. In addition, the properties of the photocatalysts before and after UV illumination were investigated, finding that part of Ti-OH on TiO2 surface transformed to Ti-O-Ti. In a nutshell, this work could provide a new insight into enhancing activity of photocatalysts and understanding the photocatalytic mechanism.

Roles of Fibroblast Growth Factor-inducible 14 in Hepatocellular Carcinoma

Li, Nan,Hu, Wen-Jun,Shi, Jie,Xue, Jie,Guo, Wei-Xing,Zhang, Yang,Guan, Dong-Xian,Liu, Shu-Peng,Cheng, Yu-Qiang,Wu, Meng-Chao,Xie, Dong,Liu, Shan-Rong,Cheng, Shu-Qun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

The prognostic value of the fibroblast growth factor-inducible 14 (Fn14) expression in hepatocellular carcinoma (HCC) is unknown. Real-time PCR (RT-PCR), western blot assays and immunohistochemistry analysis were here performed in order to compare Fn14 expressios in paired liver samples of HCC and normal liver tissue. Most of the tumor tissues expressed significantly higher levels of Fn14 compared to adjacent non-tumor tissues, with Fn14High accounting for 54.6% (142/260) of all patients. The Pearson ${\chi}^2$ test indicated that Fn14 expression was closely associated with serum alpha fetal protein (AFP) (P=0.002) and tumor number (p=0.019). Univariate and multivariate analyses revealed that along with tumor diameter and portal vein tumor thrombosis (PVTT ) type, Fn14 was an independent prognostic factor for both overall survival (OS) (HR=1.398, p=0.008) and recurrence (HR=1.541, p=0.001) rates. Fn14 overexpression HCC correlated with poor surgical outcome, and this molecule may be a candidate biomarker for prognosis as well as a target for therapy.

Polysaccharide from Polygonatum Inhibits the Proliferation of Prostate Cancer-Associated Fibroblasts Cells

Han, Shu-Yu,Hu, Ming-Hua,Qi, Guan-Yun,Ma, Chao-Xiong,Wang, Yuan-Yuan,Ma, Fang-Li,Tao, Ning,Qin, Zhi-Hai Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8

Inhibition of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Polysaccharide extracted from polygonatum can selectively inhibit the growth of prostate-CAFs (p<0.001) without inhibiting the growth of normal fibroblasts (NAFs). Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs. 3-methyl-adenine(3-MA) is an autophagy inhibitor. 3-MA was added to prostate-CAFs with polysaccharide from polygonatum to determine whether autophagy plays an important role in the restrained effect. Finally, polysaccharide from polygonatum treatment significantly increased the activation of Beclin-1 and LC3, key autophagy proteins. Polysaccharide from polygonatum stimulates autophagy of prostate-CAFs and inhibits prostate-CAF growth, indicating that a novel anti-cancer strategy involves inhibiting the growth of prostate-CAFs.

Structure Analysis of 16S rDNA Sequences from Strains of Acidithiobacillus ferrooxidans

Hong Peng,Yu Yang,Xuan Li,Guan Zhou Qiu,Xue Duan Liu,Ju Fang Huang,Yue Hua Hu 한국생활환경학회 2006 BMB Reports Vol.39 No.2

Network Intrusion Detection Based on Directed Acyclic Graph and Belief Rule Base

Bang-Cheng Zhang,Guan-Yu Hu,Zhi-Jie Zhou,You-Min Zhang,Pei-Li Qiao,Lei-Lei Chang 한국전자통신연구원 2017 ETRI Journal Vol.39 No.4

Intrusion detection is very important for network situation awareness. While a few methods have been proposed to detect network intrusion, they cannot directly and effectively utilize semi-quantitative information consisting of expert knowledge and quantitative data. Hence, this paper proposes a new detection model based on a directed acyclic graph (DAG) and a belief rule base (BRB). In the proposed model, called DAG-BRB, the DAG is employed to construct a multi-layered BRB model that can avoid explosion of combinations of rule number because of a large number of types of intrusion. To obtain the optimal parameters of the DAG-BRB model, an improved constraint covariance matrix adaption evolution strategy (CMA-ES) is developed that can effectively solve the constraint problem in the BRB. A case study was used to test the efficiency of the proposed DAG-BRB. The results showed that compared with other detection models, the DAG-BRB model has a higher detection rate and can be used in real networks.

The dynamic transcriptome of waxy maize (Zea mays L. sinensis Kulesh) during seed development

Wei Gu,Diansi Yu,Yuan Guan,Hui Wang,Tao Qin,Pingdong Sun,Yingxiong Hu,Jihui Wei,Hongjian Zheng 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Waxy maize (Zea mays L. sinensis Kulesh) is a mutant of maize (Zea mays L.) with a mutation at Waxy1 (Wx1) gene locus. The seed of waxy maize has higher viscosity compared to regular maize. By now, we know little about the expression patterns of genes that involved in the seed development of waxy maize. Objective By analyzing the transcriptome data during waxy maize seed development, we attempt to dig out the genes that may infuence the seed development of waxy maize. Methods The seeds of waxy maize inbred line SWL01 from six phases after pollination were used to do RNA-seq. Bioinformatics methods were used to analyze the expression patterns of the expressed genes, to identify the genes involved in waxy maize seed development. Results A total of 24,546 genes including 1611 transcription factors (TFs) were detected during waxy maize seed development. Coexpression analysis of expressed genes revealed the dynamic processes of waxy maize seed development. Particularly, 2457 genes including 177 TFs were specially expressed in waxy maize seed, some of which mainly involved in the process of seed dormancy and maturation. In addition, 2681, 5686, 4491, 4386, 3669 and 4624 genes were identifed to be diferential expressed genes (DEGs) at six phases compared to regular maize B73, and 113 DEGs among them may be key genes that lead the diference of seed development between waxy and regular maizes in milk stage. Conclusion In summary, we elucidated the expression patterns of expressed genes during waxy maize seed development globally. A series of genes that associated with seed development were identifed in our research, which may provide an important resource for functional study of waxy maize seed development to help molecular assisted breeding.

The Relative Influence of Diet and Physical Activity on Obesity in China

Cui Zhao-Hui,Li Yan-Ping,Di Yu-Feng,Ba Lei,Hu Xiaoqi,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to investigate the relative influence of diet and physical activity on obesity. The subjects were 155 adults aged 35-52 years from 24 neighborhood committees in 4 urban districts of Beijing (male : 78, female : 77). They were divided into normal weight, overweight and obese groups according to their BMI. The general information of the subjects was collected by interview-administered questionnaire. Dietary intake was obtained by three-day(two weekdays and one weekend day) food weighted method, physical activity was assessed by a validated combination of data obtained from activity monitors, bicycling information and activity records. There were no significant differences of age, gender, height, educational, family economic level, smoking and drinking between different groups. The proportion of flour intake was higher in obese group compared to normal weight and overweight groups, and that of vegetables is lower in obese group. The physical activity (PAL) was not significantly different between two groups of the normal, overweight and obese groups. After the adjustment for confounding factors using logistic regression model, we found that the proportion of flour intake was positively associated with obesity, while the proportion of vegetable intake was inversely associated with obesity. It is concluded that dietary patterns were associated with obesity and diets composed of more vegetables and less staple combined with physical activities could contribute to obesity prevention.

Proposal of a Pretreatment Nomogram for Predicting Local Recurrence after Intensity-Modulated Radiation Therapy in T4 Nasopharyngeal Carcinoma: A Retrospective Review of 415 Chinese Patients

Lu-Lu Zhang,Yi-Yang Li,Jiang Hu,Guan-Qun Zhou,Lei Chen,Wen-Fei Li,Ai-Hua Lin,Jun Ma,Zhen-Yu Qi,Ying Sun 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4

Purpose Local relapse-free survival (LRFS) differs widely among patients with T4 category nasopharyngeal carcinoma (NPC). We aimed to build a nomogram incorporating clinicopathological information to predict LRFS in T4 NPC after definitive intensity-modulated radiation therapy (IMRT). Materials and Methods Retrospective study of 415 Chinese patients with non-metastatic T4 NPC treated with definitive IMRT with or without chemotherapy at our cancer center between October 2009 and September 2013. The nomogram for LRFS at 3 and 5 years was generated based on multivariate Cox proportional hazards regression, and validated using bootstrap resampling, assessing discriminative performance using the concordance index (C-index) and determining calibration ability via calibration curves. Results Five-year LRFS was 88.8%. We identified and incorporated four independent prognostic factors for LRFS: ethmoid sinus invasion, primary gross tumor volume, age, and pretreatment body mass index. The C-index of the nomogram for local recurrence was 0.732 (95% confidence interval, 0.726 to 0.738), indicating excellent predictive accuracy. The calibration curve revealed excellent agreement between nomogram-predicted and observed LRFS probabilities. Risk subgroups based on total point score cutoff values enabled effective discrimination of LRFS. Conclusion This pretreatment nomogram enables clinicians to accurately predict LRFS in T4 NPC after definitive IMRT, and could help to facilitate personalized patient counselling and treatment strategies.

Positive Association of TEAD1 With Schizophrenia in a Northeast Chinese Han Population

Yang Sun,Lin Wen,Yi-Yang Luo,Wen-Juan Hu,Hui-Wen Ren,Ye Lv,Cong Zhang,Ping Gao,Li-Na Xuan,Guan-Yu Wang,Cheng-Jie Li,Zhi-Xin Xiang,Zhi-Lin Luan 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.12

Objective Schizophrenia is a complex and devastating psychiatric disorder with a strong genetic background. However, much uncertainty still exists about the role of genetic susceptibility in the pathophysiology of schizophrenia. TEA domain transcription factor 1 (TEAD1) is a transcription factor associated with neurodevelopment and has modulating effects on various nervous system diseases. In the current study, we performed a case–control association study in a Northeast Chinese Han population to explore the characteristics of pathogenic <i>TEAD1</i> polymorphisms and potential association with schizophrenia.Methods We recruited a total of 721 schizophrenia patients and 1,195 healthy controls in this study. The 9 single nucleotide polymorphisms (SNPs) in the gene region of <i>TEAD1</i> were selected and genotyped.Results The genetic association analyses showed that five SNPs (rs12289262, rs6485989, rs4415740, rs7113256, and rs1866709) were significantly different between schizophrenia patients and healthy controls in allele or/and genotype frequencies. After Bonferroni correction, the association of three SNPs (rs4415740, rs7113256, and rs1866709) with schizophrenia were still evident. Haplotype analysis revealed that two strong linkage disequilibrium blocks (rs6485989-rs4415740-rs7113256 and rs16911710-rs12364619-rs1866709) were globally associated with schizophrenia. Four haplotypes (C-C-C and T-T-T, rs6485989-rs4415740-rs7113256; G-T-A and G-T-G, rs16911710-rs12364619-rs1866709) were significantly different between schizophrenia patients and healthy controls.Conclusion The current findings indicated that the human <i>TEAD1</i> gene has a genetic association with schizophrenia in the Chinese Han population and may act as a susceptibility gene for schizophrenia.