http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
결핵균과 비결핵성항산균 검출에 Real-time PCR의 유용성
윤은영 ( Eun Young Yun ),조수희 ( Su Hee Cho ),고세일 ( Se Il Go ),백종하 ( Jong Ha Baek ),김유은 ( You Eun Kim ),마정은 ( Jeong Eun Ma ),이기동 ( Gi Dong Lee ),조유지 ( Yu Ji Cho ),정이영 ( Yi Yeong Jeong ),김호철 ( Ho Cheol Kim 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.69 No.4
Background: The purpose of this study was to evaluate recently developed real-time polymerase chain reaction (PCR) assay kit to detect Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) in respiratory specimens. Methods: We assessed the positive rate of the real-time PCR assay to detect MTB and NTM in 87 culture-positive specimens (37 sputum, 50 bronchial washing), which were performed real-time PCR by using Real-QTM MTB&NTM Kit from January 2009 to June 2009, at Gyeongsang University Hospital. To compare the efficacy with the TB-PCR assay, we evaluated 63 culture-positive specimens (19 sputum, 44 bronchial washing) for MTB or NTM, which were performed TB-PCR by using ABSOLUTETM MTB II PCR Kit from March 2008 to August 2008. Results: Among 87 specimens tested using real-time PCR, MTB and NTM were cultured in 58 and 29, respectively. The positive rate of real-time PCR assay to detect MTB was 71% (22/31) and 92.6% (25/27) in AFB stain-negative and stain-positive specimens. For NTM, the positive rate of real-time PCR was 11.1% (2/18) and 72.7% (8/11) in AFB stain-negative and stain-positive specimens. Among 63 specimens performed using TB-PCR, MTB and NTM were cultured in 46 and 17, respectively. The positive rate of TB-PCR was 61.7% (21/34) and 100% (12/12) in AFB stain-negative and stain-positive specimens. TB-PCR was negative in all NTM-cultured 17 specimens. Conclusion: TB/NTM real-time PCR assay is useful to differentiate MTB and NTM in AFB stain-positive respiratory specimens and it is as effective in detecting MTB with TB-PCR.
Expression Pattern of Potato (Solanum tuberosum) Genes under Cold Stress by using cDNA Microarray
Kim, Dool-Yi,Lee, Hye-Eun,Yi, Ki-Wan,Han, Sang-Eun,Kwon, Hawk-Bin,Go, Seung-Joo,Byun, Myung-Ok 한국유전학회 2003 Genes & Genomics Vol.25 No.4
Exploring the tolerant mechanism of crop plants to abiotic stresses causing decreased yield is one important research undertaking. The first step to achieve this goal is through isolation of genes that are regulated by such stresses. This study attempted to isolate genes that respond to cold temperature, one of the major abiotic stresses of crops. First, cDNA library with RNA extracted from vegetative leaf, stem, and root of potato plants treated at 4˚C and 25˚C for 8 hrs was constructed. To evaluate transcriptional regulation by abiotic stresses like cold (4˚C), salt (250 mM NaCl), drought (air drying for 24 hrs), and pathogen treatment (zoospore treatment of Phytophthora infestans), 100 genes were spotted onto DNA chip. Results showed that seven clones of cold stress protein were up-regulated by cold treatment, while six clones of ADP-ribosylation factor-like protein were up-regulated by salt treatment. Pathogen up-regulated the expression of ubiquitin carrier and drought stress up-regulated the expressions of cysteine protease and photosystem Ⅱ homologue. Expression of some clones showing up-regulation by cold treatment in cDNA microarray was compared with that of Northern blot analysis. Although the results of the microarray analysis were not completely consistent with that of the Northern blot analysis, there was a very strong relationship between the two analyses.
Nasar Uddin Ahmed,Go-Eun Yi,Jong-In Park,Hee-Jeong Jung,Senthil Kumar Thamilarasan,Mi-Young Chung,Ill-Sup Nou 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Flavonoids are divided into several structural classes, including anthocyanins, which provide flower and leaf colors and other derivatives with diverse roles in plant development and interactions with the environment. This study characterized four Anthocyanidin Synthase (ANS) genes of Brassica rapa, a structural gene of anthocyanin biosynthetic pathway, and investigated their association with cold and freezing tolerance in B. rapa. Sequences of these genes were analyzed and compared with similar types of gene sequences of other species and found a high degree of homology with their respective functions. In the organ specific expression analysis, these genes showed expression only in the colored portion of leaves of different lines of B. rapa. On the other hand, BrANS genes also showed differential expression with certain time course of cold stress treatment in B. rapa. Thus, the above results suggest probable association of these genes with anthocyanin biosynthesis and cold and freezing tolerance and might be useful resources for developing cold resistant Brassica crops with desirable colors as well. The present work may help explore the molecular mechanism that regulates anthocyanin biosynthesis and its response to abiotic stress at the transcriptional level in plants.
한의학 칠정(七情)에 기반을 둔 핵심감정평가척도 개발을 위한 예비 연구
이고은 ( Go Eun Lee ),박보영 ( Bo Young Park ),김향이 ( Hang Yi Kim ),유영수 ( Yeoung Su Lyu ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2014 동의신경정신과학회지 Vol.25 No.1
Objectives: The aim of this study is to investigate the core emotion assessment instrument based on the Chiljeong. Methods: We searched for a literature review of oriental medicine and established the operational definition. Based on the existing psychological scale measurement, we selected the items and analyzed the reliability through a two-step preliminary study. Results: 1) Through the analysis of reliability after the two-step preliminary study (first: 170 items, n=63, second: 152 items, n=42), we had excellent internal consistency (Cronbach``s alpha > 0.70) as well as good test-retest reliability (>0.70). Thus, we demonstrated that this scale had reliability. 2) Analyzing the correlation of each emotion, we had a comparatively strong positive-correlation (>0.6), except for Hui, and found a particularly high correlation of Sa, U and Bi. Conclusions: We developed the final 141 items of the core emotion assessment instrument through the two-time preliminary study. In the future, the main study on the clinical and non clinical groups of various ages should be continued.
( Hyung Eun Son ),( Ji Young Ryu ),( Suryeong Go ),( Youngjin Yi ),( Kipyo Kim ),( Yoon Kyu Oh ),( Kook-hwan Oh ),( Ho Jun Chin ) 대한신장학회 2020 Kidney Research and Clinical Practice Vol.39 No.1
Background: The significance of ambulatory blood pressure (ABP) in Korean patients with chronic kidney disease (CKD) in relation to renal outcome or death remains unclear. We investigated the role of ABP in predicting end-stage renal disease or death in patients with CKD. Methods: We enrolled 387 patients with hypertension and CKD who underwent ABP monitoring and were followed for 1 year. Data on clinical parameters and outcomes from August 2014 to May 2018 were retrospectively collected. The composite endpoint was end-stage renal disease or death. Patients were grouped according to the mean ABP. Results: There were 66 endpoint events, 52 end-stage renal disease cases, and 15 mortalities. Among all patients, one developed end-stage renal disease and died. Mean ABP in the systolic and diastolic phases were risk factors for the development of composite outcome with hazard ratios of 1.03 (95% confidence interval [CI], 1.01-1.04; P < 0.001) and 1.04 (95% CI, 1.02-1.07; P = 0.001) for every 1 mmHg increase in BP, respectively. Patients with mean ABP between 125/75 and 130/80 mmHg had a 2.56-fold higher risk for the development of composite outcome (95% CI, 0.72-9.12; P = 0.147) as compared to those with mean ABP ≤ 125/75 mmHg. Patients with mean ABP ≥ 130/80 mmHg had a 4.79-fold higher risk (95% CI, 1.68-13.70; P = 0.003) compared to those with mean ABP ≤ 125/75 mmHg. Office blood pressure (OBP) was not a risk factor for the composite outcome when adjusted for covariates. Conclusion: In contrast to OBP, ABP was a significant risk factor for end-stage renal disease or death in CKD patients.
Cell Motility Is Decreased in Macrophages Activated by Cancer Cell-Conditioned Medium
( Ahreum Go ),( Yun Kyoung Ryu ),( Jae Wook Lee ),( Eun Yi Moon ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.6
Macrophages play a role in innate immune responses to various foreign antigens. Many products from primary tumors infl uence the activation and transmigration of macrophages. Here, we investigated a migration of macrophages stimulated with cancer cell culture-conditioned medium (CM). Macrophage activation by treatment with CM of B16F10 cells were judged by the increase in protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2). The location where macrophages were at 4 h-incubation with control medium or CM was different from where they were at 5 h-incubation in culture dish. Percentage of superimposed macrophages at every 1 h interval was gradually increased by CM treatment as compared to control. Total coverage of migrated track expressed in coordinates was smaller and total distance of migration was shorter in CM-treated macrophages than that in control. Rac1 activity in CM-treated macrophages was also decreased as compared to that in control. When macrophages were treated with CM in the presence of dexamethasone (Dex), an increase in COX2 protein levels, and a decrease in Rac1 activity and total coverage of migration were reversed. In the meanwhile, biphasic changes were detected by Dex treatment in section distance of migration at each time interval, which was more decreased at early time and then increased at later time. Taken together, data demonstrate that macrophage motility could be reduced in accordance with activation in response to cancer cell products. It suggests that macrophage motility could be a novel marker to monitor cancer-associated infl ammatory diseases and the effi cacy of anti-infl ammatory agents.
화병환자의 불면증상에 대한 침치료 유효성 평가연구: 환자-평가자 눈가림, 무작위배정, 일반대조군 임상시험
이고은 ( Go Eun Lee ),김남권 ( Nam Kwon Kim ),김향이 ( Hang Yi Kim ),강형원 ( Hyung Won Kang ) 대한한방신경정신과학회 2012 동의신경정신과학회지 Vol.23 No.1
Objectives: The purpose of this research is to examine the effects of acupuncture treatment on Hwa-byung patient`s insomnia. Methods: The study was performed through a patient-assessor blind, randomized, placebo-controlled trial in which the volunteers, data collectors and analysts were unaware of individuals who were receiving the treatment. A total of thirty-seven volunteers were divided into 2 groups. 18 subjects were placed into a trial group and 19 subjects into a control group by a randomization table. The trial group was treated on bilateral Shigu, Ahnmyun, B62 (Shinmaek), and K6 (Chohae), while the control group was not given any other treatment. The ISI(Insomnia Severity Scale) was measured as the 1st evaluative instrument, and then a comparative analysis was conducted by comparing the results with those measured by a Likert scale for major symptoms of Hwa-byung, PSQI, Hwa-byung scale, BDI, STAI, and STAXI. Results: In the ISI, PSQI, and the Likert scale regarding major symptoms of Hwa-byung, Hwa-byung scale, BDI, and STAI, more significant decreases were found in those concerning the trial group compared to those of the control group from a statistical perspective. However, in the STAXI, regarding the trial group, there was no significant decrease compared to the control group. Conclusions: The results suggest that acupuncture treatment is effective in the treatment regarding Hwa-byung patients who suffer from insomnia.