亞黃酸가스에 爆露된 생쥐 기도상피의 組織學的 觀察

洪仁杓,金武剛 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

In order to observe histologically the injurious effects of mice exposed to sulfur dioxide, mice were exposed to 25 and 50 ppm, of sulfur dioxide for 10 days respectively. The results obtained are as follows : 1. In the clinical signs, the exposed mice become restless and severely dyspnic, rubbing their noses and eyes immediately after the biginning of exposure to sulfur dioxide. 2. Microscopical changes in the upper--and lower-respiratory tract were observed focal loss of cilia and goblet cell hyperplasia after 2 days of exposure to 25 and 50 ppm. , more extensive loss of cilia, disappearance of goblet cells, necrosis and extrusion of cells, and irregular rows of nuclei of basal, supporting, and olfactory cells after 4 and 6 days of exposure, and basal cell hyperplasia and the development of squamous epithelium after 8 and 10 days.

韓國人 手掌皮膚 Meissner 小體의 量的 變化에 關한 硏究

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

In order to observe the quantitative changes in Meissner's tactile corpuscles caused by aging and in different areas, 3×5 ㎟ punch excision specimens in the palm of the right and left hand from the 46 cadavers (male 35, female 11) were collected, sectioned serially, stained by the methylene blue and observed with light microscope and then analyzed a numerical changes in the palm of the right and left hand and in the site 6 and 7 in the palm of the hand. The results are summerized as follows: 1. The mean concentration of Meissner's corpuscles in the palm of the right hand was 2.92 per square millimeter and in the palm of the left hand was 5.17. There was appeared the mean concentration of Meissner's corpuscles in the palm of the hand was greater in the left than in the right. 2. In the site 6, the mean concentration of Meissner's corpuscles was 3.93 per square millimeter in the age group 21 to 50 years, 2.96 in the 51 to 60 years, 2.52 in the 61 to 83 years. There was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 3. In the site 7, the mean concentration of Meissner's corpuscles was 5.51 per square millimeter in the age group 21 to 50 years, 4.98 in the 51 to 60 years, 4.94 in the 61 to 78 years. Also, there was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 4. In the site 6, the mean concentration of Meissner's corpuscles in the palm of the right hand was 3.57 per square millimeter in the age group 21 to 50 years, 2.74 in the 61 to 78 years and in the palm of the left hand was 3.07 in the 21 to 60 years, 2.24 in the 61 to 83 years. There was appeared the mean concentration of Meissner's corpuscles in the site 6 of the palm was greater in the right than in the left. 5. In tie site 7, the mean concentration of Meissner's corpuscles in the palm of the right hand was 5.12 per square millimeter in the age group 21 to 78 years and in the palm of the left hand was 5.13 in the 21 to 78 years. There was appeared the mean concentration of Meissner's corpuscles in the site 7 of the palm was a little greater in the left than in the right.

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

생쥐 鼻中隔 各種 粘膜上皮 및 粘膜下腺의 分布樣狀과 그 表面積에 관한 硏究

金東煥,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

In order to set the distribution pattern and surface area of the mucosal epithelium and submucosal gland in the mice nasal septum. 3-month-old healthy 6 mice weighing 25gm approximately have been contributed for this research. For the mapping of the each nasal epithelium and submucosal gland in the submicroscopic level, the routine paraffin block of the mice nasal septum were cut as the 10㎛ thick at 110㎛ intervals. Consecutive 2 slides were stained with H-E and PAS and reconstructed in form of maps representing the structure in a sagittal plane. The results obtained from this research were summarized as follows. 1. The unilateral nasal surface epithelial area of the mice nasal cavity was 31.23㎟ 2. The areas of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium which compose the unilateral nasal epithelium were 4.47㎟(14.3%), 12.7㎟(40.8%), and 14.02㎟(44.9%), respectively. 3. Submucosal glands in the mice nasal septum were divided into the PAS-positive, PAS-negative and Bowman's glands by the PAS staining. 4. The surface area of the PAS-positive glands in the nasal unilateral septum was 5.23㎟ which is located in the central area of the respiratory portion. 5. The surface area of the PAS-negative glands in the nasal unilateral septum was 5.05㎟ which is located above and below the PAS-positive gland. 6. The surface area of the Bowman's glands was 12.1 ㎟ which was located in the olfactory region. 7. In the vestibular region., 3 ducts of PAS-negative glands of the respiratory region are found, and below the nasal septum there is the infraseptal gland(PAS-negative).

오리 鼻腔 및 鼻粘膜上皮의 計測에 관한 硏究

金世仁,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The area of the various epithelial types linging the nasal navity of each six number in 1-week-old and 10-week-old adult ducks has been made for the light microscopic research. Transverse sections of 10 microns thick in each specimen were made continuously from the anterior end of nostril to the posterior end of pharyngeal inlet and specimen samples were selected at 0.13mm(1-week-old) and 0.17mm(10-week-old) intervals from the anterior end of the nasal cavity to the posterior end of pharyngeal inlet. The results obtained from research were summarized as follows. 1. The nasal cavity is lined with stratified squamous, respiratory and olfactory epithelium. The thickness of stratified squamous, respiratory and olfactory epithelium is 10-100㎛, 8-75㎛ and 50㎛ respectively in 1-week-old ducks, and 25-175㎛, 20-125㎛ and 66㎛ respectively in 10-week-old ducks. 2. The unilateral volumes of the nasal cavity in 1-week-old and 10-week-old ducks were 21.73±2.64㎟, 1,692.44±171.57㎟ respectively. 3. The total unilateral surface areas of the nasal cavity in 1-week-old and 10-week-old ducks were 70.44±4.92㎟, 1,772.45±94.10㎟ respectively. 4. Each area of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium in unilateral nasal cavity is 14.82±1.85㎟ (21.1%), 46.86±3.20㎟ (66.5%) and 8.76±0.40㎟ (12.4%) in the 1-week-old ducks, and 348.23±40.02㎟ (19.7%), 1,282.31±47.03㎟ (72.3%) and 141.90±12.43㎟ (8.0%) in the 10-week-old ducks. Ducks are assumed as a microsmatic animals by the composition of the lining epithelium of the nasal cavity in ducks.

오리 網膜에 存在하는 神經節細胞의 數와 分布에 關한 硏究

姜翰秀,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The number and distribution of the retinal ganglion cells in the 1-week-old and 7-week-old ducks were determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. In the 1-week-old and 7-week-old ducks, the areas of the retinas were 269.4㎟ and 357.2㎟, respectively, and the total cell numbers 1,742,800 and 1,808,800, respectively. 2. The ganglion cells based upon soma diameter were classified into 3 groups such as small (below 10㎛), medium (10-20㎛) and large (above 20㎛) ganglion cells. In 1-week-old duck, the relative frequencies of these 3 groups were 91.0%, 8.5% and 0.5%, respectively, and in 7-week-old duck, 83.7% 14.7% and 1.6%, respectively. 3. Area centralis was located at the distance of 1.6mm (1-week-old) and 2.0mm(7-week-old) nasal side from the anterior tip of the pecten. The number of ganglion cells in the area centralis was 11,000 cells/㎟ (1-week-old) and 12,000 cells/㎟ (7-week-old). 4. There was a gradual decrease in density of retinal ganglion cells extending from area centralis to the periphery of retina. 5. The density of the large (above 20㎛) ganglion cells was higher in the peripheral regions than in the center of retina. Higher densities were noted in the superior-temporal region in 7-week-old duck, and in the nasal region in 1-week-old duck than in the other regions. 6. The direction of the beak tip is approximately 30° in 1-week-old and 26° in 7-week-old duck from the angle of the pecten.

오리 선위 점액분비세포의 점액질에 대한 조직화학적 연구

류시윤,김홍선,김무강,신태균,이영호 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

Histochemical sequential staining techniques, PAS, alcian blue pH 2.5 and pH 0.4, were applied to sections from the proventriculus of ducks to observe the histochemical properties of epithelial mucosubstances in the proventriculus of mallard and white Pekin ducks. The whole proventriculus obtained were divided systemically cross strips and processed routinely for histologic and histochemical study. Base on the histologic characteristics, we designated as upper portion which is the distal region of the esophageal-proventriculus junction, as middle portion, and as lower portion which is the proximal region of the proventricular-isthmus junction. The results obtained are as follows 1. Generally in the surface and foveolar epithelia showed the presence of the mixture of neutral and acid mucosubstances. 2. In the tubular gland showed the acidic nonsulfated and sulfated mucosubstance in addition to neutral mucosubstances with occasional neutral and acidic nonsulfated mucosubstances. 3. In the compound gland neutral mucosubstance with scant amounts of acidic mucosubstances or with lack of it were demonstrated. 4. There were recognized differences between speies in the staining intensity of all three types of mucosubstances. 5. There were recognized topographic difference in the acidity of epithelial mucosubstances according to portion.

원위부 총수담관에 발생한 편평상피세포암 1예 보고

김강미(Gang Mi Kim),최기홍(Gi Hong Choi),김동현(Dong Hyun Kim),강창무(Chang Moo Kang),이우정(Woo Jung Lee) 한국간담췌외과학회 2008 한국간담췌외과학회지 Vol.12 No.3

Squamous cell carcinoma of the extrahepatic bile duct is very rare. Only limited information is available concerning its pathogenesis and prognosis, although it is reported to be associated with choledocolithiasis, recurrent pyogenic cholangitis, clonorchiasis, choledochal cyst formation, and primary sclerosing cholangitis. Therefore, we present a case of squamous cell carcinoma of the distal common bile duct. A 64-year-old male patient presented with abdominal pain and obstructive jaundice. Computed tomography and endoscopic retrograde cholangiography showed thickening of the wall and a segmental stricture of the distal common bile duct, and the biopsy confirmed poorly differentiated carcinoma with marked squamous differentiation. The patient underwent a pylorus-preserving pancreaticoduodencetomy. Histological examination revealed that the tumor invaded the pancreas and metastasized to regional lymph nodes (pT3N1M0). The patient developed multiple liver metastases 30 days later and died of rapidlyprogressive metastatic disease to the liver 5 months following resection. Squamous cell carcinoma of the distal bile duct with lymph node metastasis had a very poor prognosis, even though curative resection was performed.

鷄胎器官性長에 미치는 Mitoycin C의 影響에 關한 硏究

金武剛,金弘善,崔大卿,趙聲煥,金源式 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Administration of Mitomycin C into various species of animals has widely been reported since last decade, however, none of them were utilized a growth formula proposed by Zimmerman and Huxley. In this paper we attempt to analyze the specific growth rate in general and organ relative growth rate using the chick embryo under the influence of Mitomycin C which is well known as an antibacterial, antiviral and antitumor by the mechanism of the alkylation. Congenital malformation of chick embryo were not considered. On the sixth day incubation, 8㎍ and 16㎍ of Mitomycin C were injected into the chorioallantoic and the egg was broken for weighing the body weight and individual organs such as brain, heart and lung on the day of 12th, 14th, 16th and 18th. The effects of the growth were analyzed by the analysis of variance method. The parameters included in growth quantity were obtained by the parameters included in growth quantity were obtained by the mathod of the last square using the measured weight, the formulas applied to analysis growth quantity and relative growth were : y=a+bt+ct^2, y=bx^2, and the results this obtained were summarized as follows : When injected with Mitomycin C, the chick embryos were observed with remarkable growth inhibition in all groups of the body, brain, lung and 14th groups of heart (p<0.005) and 12th, 16th, 18th groups of heart(p<0.05). Growth rate was increased with the development in the 8㎍ injected groups of the body, lung and 16㎍ injected groups of the lung, on the other hand, it was decreased in the both injected group of the brain and heart. The specific growth rate of all groups showed tendency of decrease in 8㎍ and 16㎍ Injected groups. The coefficient of relative growth of injected groups to control groups in the various organs showed positive allometry. The growth center of the growth gradient of all injected groups to control groups were on the eighteenth day.

간세포암의 근치적 절제 후 간내 재발암의 위험 인자 분석

김강미(Gang Mi Kim),최기홍(Gi Hong Choi),한대훈(Dai Hoon Han),김동현(Dong Hyun Kim),강창무(Chang Moo Kang),최진섭(Jin Sub Choi),박준용(Jun Yong Park),김도영(Do Yong Kim),한광협(Kwang-Hyub Han),전재윤(Chae Yoon Chon),박영년(Young Nyun 한국간담췌외과학회 2008 한국간담췌외과학회지 Vol.12 No.4

Purpose: Intrahepatic recurrent HCC has been classified according to location, the time to recurrence and the pattern of presentation. The purpose of this study is to classify intrahepatic recurrent HCCs into subgroups that have relatively similar recurrent patterns and to identify the risk factors for each recurrent type. Methods: A total of 353 patients were retrospectively studied. Intrahepatic recurrent HCC was classified into nodular recurrence (<4 nodules; type I), multinodular-diffuse recurrence (≥4 nodules; type II) and infiltrative recurrence (type III). The cut-off time between early and late recurrence was chosen to be 12 months following hepatectomy. Results: Among the 134 patients with only intrahepatic recurrence, 94 were type I, 27 were type II and 13 were type III. The median survival time following the recurrence of types I, II and III were 55, 16 and 8 months, respectively. As determined by multivariate analysis, perioperative transfusion and indocyanine green retention at 15 minutes (ICG R 15 >10%) were the independent risk factors for type I; an ICG R 15>10%, microvessel invasion and intrahepatic metastasis were the independent risk factors for type II; an ICG R 15>10% and microscopic portal vein invasion were the independent risk factors for type III. Multivariate analysis revealed that the prognosis of patients with IHR was associated with the recurrent types, the time to recurrence and the serum albumin level at the initial presentation. Following multivariate analysis, an ICG R 15>10% and intrahepatic metastasis were the independent risk factors for early type I recurrence; perioperative transfusion and a higher grade of hepatitis activity were the independent risk factors for late type I recurrence. Conclusions: The recurrent types and the time to recurrence may help us to predict the cellular origin of intrahepatic recurrent HCC and the prognosis of the patients who suffer with intrahepatic recurrent HCC.