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Fang Shen,Wan-li Huang,Bao-ping Xing,Xiang Fang,Mei Feng,Chun-ming Jiang 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.11
Objective Recent studies have indicated the possibility that genistein may improve depression via regulating the expression of miR- 221/222. This study is to explore whether genistein could improve depression by altering miR-221/222 levels and investigate the possible mechanisms involved in the improvement effect of genistein. Methods The animal model of depression was established through unpredictable chronic mild stress. Nest building test and splash test were adapted to evaluate the effects of genistein on depressive symptoms in mice. qRT-PCR and western blot analysis were used to detect the expression of miR-221/222 and connexin 43 (Cx43) in the prefrontal cortex of the mice. In vitro, U87-MG astrocytes were treated with genistein and the expression of miR-221/222 and Cx43 was measured. The dual-luciferase reporter assay was used to verify whether Cx43 was a direct target of miR-221/222. Results The behavioral tests showed that genistein could significantly reduce depression symptoms of mice, and this remission was not affected by gender. Genistein in vivo and in vitro could reduce increased levels of miR-221 and miR-222 in the prefrontal cortex of depressed mice, while upregulate Cx43 expression. Dual-luciferase reporter assay suggested Cx43 was directly regulated by miR-221/222 in astrocytes. Conclusion Genistein can play its antidepressant effect through down-regulating miR-221/222 by targeting Cx43.
Fang Shen,Wan-li Huang,Bao-ping Xing,Xiang Fang,Mei Feng,Chun-ming Jiang 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.10
Objective: Recent studies have indicated the possibility that genistein may improve depression via regulating the expression of miR-221/222. This study is to explore whether genistein could improve depression by altering miR-221/222 levels and investigate the possible mechanisms involved in the improvement effect of genistein. Methods: The animal model of depression was established through unpredictable chronic mild stress. Nest building test and splash test were adapted to evaluate the effects of genistein on depressive symptoms in mice. qRT-PCR and western blot analysis were used to detect the expression of miR-221/222 and connexin 43 (Cx43) in the prefrontal cortex of the mice. In vitro, U87-MG astrocytes were treated with genistein and the expression of miR-221/222 and Cx43 was measured. The dual-luciferase reporter assay was used to verify whether Cx43 was a direct target of miR-221/222. Results: The behavioral tests showed that genistein could significantly reduce depression symptoms of mice, and this remission was not affected by gender. Genistein in vivo and in vitro could reduce increased levels of miR-221 and miR-222 in the prefrontal cortex of depressed mice, while upregulate Cx43 expression. Dual-luciferase reporter assay suggested Cx43 was directly regulated by miR-221/222 in astrocytes. Conclusion: Genistein can play its antidepressant effect through down-regulating miR-221/222 by targeting Cx43.
Ya-fang He,Li Hua,Yi-xiao Bao,Quan-hua Liu,Yi Chu,Ding-zhu Fang 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6
Purpose: Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smoothmuscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the presentstudy, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparisonwith wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs). Methods: Culture supernatants and cell proteins were collectedfrom cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 h. Eotaxin released into hBSMC culture medium was determinedby ELISA. The expression levels of the high-affinity IgE receptor (FcεRI) α-chain, smooth muscle-specific actin alpha chain (α-SMA), smoothmuscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots. Results: Compared with WT IL-13, treatmentwith the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expressionlevels of α-SMA, SmMHC, calreticulin, and FcεRI α-chain. Conclusions: The results of the present study suggenst that the IL-13 R110Q variantmay enhance enhanced functional activities in hBSMCs.
Study on Corner Reflectors Identification in Highway Deformation Monitoring
Xue Min Xing,De Bao Wen,Fang Bin Zhou 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.12
CRInSAR is a newly developed technique to monitor ground deformation. In CRInSAR algorithm, the identification of Corner Reflectors in SAR images is necessary. Due to the uncertainty of traditional identification method, a new method based on the intensity and correlation coefficient of each CR candidates in SAR images is presented. The method has been successfully used to determine the locations of 11 CRs installed along a highway in six SAR images over the study area. The results show that the identification accuracy of the new method is about 1 pixel. It is effective and reliable especially in the area with lots of lightspots around the CR points. The method proposed can play important role in the highway deformation monitoring within CRInSAR algorithm.
Preparation and Gas Permeability of ZIF-7 Membranes Prepared via Two-step Crystallization Technique
Fang Li,Qi Ming Li,Xin Xia Bao,Jian Zhou Gui,Xiao Fei Yu 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.3
Abstract - Continuous and dense ZIF-7 membranes were successfully synthesized on α-Al2O3 porous substrate via two-step crystallization technique. ZIF-7 seeding layer was first deposited on porous α-Al2O3 substrate by in-situ low temperature crystallization, and then ZIF-7 membrane layer can be grown through the secondary high-temperature crystallization. Two synthesis solutions with different concentration were used to prepare ZIF-7 seeding layer and membrane layer on porous α-Al2O3 substrate, respectively. As a result, a continuous and defect-free ZIF-7 membrane layer can be prepared on porous α-Al2O3 substrate, as confirmed by scanning electron microscope. XRD characterization shows that the resulting membrane layer is composed of pure ZIF-7 phase without any impurity. A single gas permeation test of H2, O2, CH4 or CO2 was conducted based on our prepared ZIF-7 membrane. The ZIF-7 membrane exhibited excellent H2 molecular sieving properties due to its suitable pore aperture and defect-free membrane layer.
Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell
Bao-Qin Liu,Hua-Qin Wang,Xin Meng,Chao Li,Yan-Yan Gao,Ning Li,Xiao-Fang Niu,Yifu Guan 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.9
Glucosamine, a naturally occurring amino monosaccharide,has been reported to play a role in the regulation of apoptosis more than half century. However the effect of glucosamine on tumor cells and the involved molecular mechanisms have not been thoroughly investigated. Glucosamine enters the hexosamine biosynthetic pathway (HBP) downstream of the rate-limiting step catalyzed by the GFAT (glutamine:fluctose-6-phosphate amidotransferase), providing UDPGlcNAc substrates for O-linked β-N-acetylglucosamine (O-GlcNAc) protein modification. Considering that O-GlcNAc modification of proteasome subunits inhibits its activity, we examined whether glucosamine induces growth inhibition via affecting proteasomal activity. In the present study, we found glucosamine inhibited proteasomal activity and the proliferation of ALVA41 prostate cancer cells. The inhibition of proteasomal activity results in the accumulation of ubiquitinated proteins, followed by induction of apoptosis. In addition, we demonstrated that glucosamine downregulated proteasome activator PA28γ and overexpression of PA28γ rescued the proteasomal activity and growth inhibition mediated by glucosamine. We further demonstrated that inhibition of O-GlcNAc abrogated PA28γ suppression induced by glucosamine. These findings suggest that glucosamine may inhibit growth of ALVA41 cancer cells through downregulation of PA28γ and inhibition of proteasomal activity via O-GlcNAc modification.
Roles of microRNA-206 in Osteosarcoma Pathogenesis and Progression
Bao, Yun-Ping,Yi, Yang,Peng, Li-Lin,Fang, Jing,Liu, Ke-Bin,Li, Wu-Zhou,Luo, Hua-Song Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
Backgroud and Aims: MicroRNA-206 has proven to be down-regulated in many human malignancies in correlation with tumour progression. Our study aimed to characterize miR-206 contributions to initiation and malignant progression of human osteosarcoma. Methods: MiR-206 expression was detected in human osteosarcoma cell 1ine MG63, human normal osteoblastic cell line hFOB 1.19, and paired osteosarcoma and normal adjacent tissues from 65 patients using quantitative RT-PCR. Relationships of miR-206 levels to clinicopathological characteristics were also investigated. Moreover, miR-206 mimics and negative control siRNA were transfected into MG63 cells to observe effects on cell viability, apoptosis, invasion and migration. Results: We found that miR-206 was down-regulated in the osteosarcoma cell line MG63 and primary tumor samples, and decreased miR-206 expression was significantly associated with advanced clinical stage, T classification, metastasis and poor histological differentiation. Additionally, transfection of miR-206 mimics could reduce MG-63 cell viability, promote cell apoptosis, and inhibit cell invasion and migration. Conclusions: These findings indicate that miR-206 may have a key role in osteosarcoma pathogenesis and development. It could serve as a useful biomarker for prediction of osteosarcoma progression, and provide a potential target for gene therapy.
Bao, Xiaohua,Di, Chong,Fang, Yong The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.1
Generally, closed slots are adopted to reduce the water friction loss in both the stator and the rotor of water filling submersible motor due to the special environment of operation. One of the obvious differences between the traditional induction motors and water filling submersible motors is that the submersible motors only need relatively smaller starting torque. This paper aims to analyze the slot leakage reactance of water filling submersible motor during starting transient operation. An improved analytical method which considered the magnetic saturation of the slot bridge and the skin effect of rotor bars is proposed. The slot permeance factor which has a direct impact on the slot leakage reactance is calculated. Then finite element models with different stator slot types are constructed and search coils are introduced to measure the slot flux linkage. Moreover, the starting performances of the models with two typical stator slots are compared and the flux leakage characteristics are obtained. Finally, the results obtained by finite element method are very close to the results obtained by analytical method.