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Changes of Cytosolic $Ca^{2+}$ under Metabolic Inhibition in Isolated Rat Ventricular Myocytes
Kang, Sung-Hyun,Kim, Na-Ri,Joo, Hyun,Youm, Jae-Boum,Park, Won-Sun,Warda, Mohamed,Kim, Hyung-Kyu,Von Cuong, Dang,Kim, Tae-Ho,Kim, Eui-Yong,Han, Jin The Korean Society of Pharmacology 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.5
To characterize cytosolic $Ca^{2+}$ fluctuations under metabolic inhibition, rat ventricular myocytes were exposed to $200{\mu}M$ 2,4-dinitrophenol (DNP), and mitochondrial $Ca^{2+}$, mitochondrial membrane potential (${\Delta}{\Psi}m$), and cytosolic $Ca^{2+}$ were measured, using Rhod-2 AM, TMRE, and Fluo-4 AM fluorescent dyes, respectively, by Laser Scanning Confocal Microscopy (LSCM). Furthermore, the role of sarcolemmal $Na^+$/$Ca^{2+}$ exchange (NCX) in cytosolic $Ca^{2+}$ efflux was studied in KB-R7943 and $Na^+$-free normal Tyrode's solution (143 mM LiCl ). When DNP was applied to cells loaded with Fluo-4 AM, Fluo-4 AM fluorescence intensity initially increased by $70{\pm}10$% within $70{\pm}10$ s, and later by $400{\pm}200$% at $850{\pm}45$ s. Fluorescence intensity of both Rhod-2 AM and TMRE were initially decreased by DNP, coincident with the initial increase of Fluo-4 AM fluorescence intensity. When sarcoplasmic reticulum (SR) $Ca^{2+}$ was depleted by $1{\mu}M thapsigargin plus $10{\mu}M ryanodine, the initial increase of Fluo-4 AM fluorescence intensity was unaffected, however, the subsequent progressive increase was abolished. KB-R7943 delayed both the first and the second phases of cytosolic $Ca^{2+}$ overload, while $Na^+$-free solution accelerated the second. The above results suggest that: 1) the initial rise in cytosolic $Ca^{2+}$ under DNP results from mitochondrial depolarization; 2) the secondary increase is caused by progressive $Ca^{2+}$ release from SR; 3) NCX plays an important role in transient cytosolic $Ca^{2+}$ shifts under metabolic inhibition with DNP.
Sang-Yun Lee,Eui-Jun Min,Hyo-Jeong Yoon,Kanphassorn Wimonmuang,Hee-Kyoung Kang,Young-Sang Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The fruits of Prunus mume containing high organic acid is a well-being food traditionally used at home and widely industrialized in Korea. Seeds of Prunus mume, however, also contain toxic cyanide called ‘amygdalin’ which may cause headache, food poisoning and even death. To develop a rapid and efficient analysis method to quantify amygdalin content, Prunus mume seeds were extracted in MeOH under different temperature (25-70℃), duration (10-240 min), multiple extraction (1-2 times) and filtration conditions and resultant extraction efficiencies were compared. Optimized analysis conditions were as follows: 100 mg of dried and pulverized seed were extracted in 10 mL MeOH at 60℃ for 30 min, filtered through Na2SO4, concentrated by using a rotary evaporator, and hexane-water solvent partitioning, collected water layer volume was adjusted to 20 mL prior to injection into an HPLC. Validation of developed method exhibited acceptable linearity (R²=0.9999, 25-500 ppm), accuracy (87-112%, recovery), repeatability (3.8%, RSD), reproducibility (3.6% RSD) and limit of detection (LOD) and quantification (LOQ) were 0.28 ppm and 0.84 ppm, respectively.
KANG, HYUN AH,KIM, JEONG-YOON,KO, SU-MIN,PARK, CHEON SEOK,RYU, D. Y.,SOHN, JUNG-HOON,CHOI, EUI-SUNG,RHEE, SANG-KI 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPases. The cloned gene product exhibits 60·3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P-type Ca^2+ -ATPase. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession Number U92083. ⓒ 1998 John Wiley & Sons, Ltd.
Large-area, Multipixel Photonic Skin Based On a Shape-memory and Responsive Cellulose Derivative
Sang-Hyeon Lee(이상현),Kahyun Sun(선가현),Minho Seong(성민호),Insol Hwang(황인솔),Minsu Kang(강민수),Jaeil Kim(김재일),Geonjun Choi(최건준),Hyejin Jang(장혜진),Seongjin Park(박성진),Hoon Eui Jeong(정훈의) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12
Eui Kyu Chie,Kyung Hwan Shin,김대용,Tae-Hyun Kim,Han-Sung Kang,Eun Sook Lee,조관호 한국유방암학회 2009 Journal of breast cancer Vol.12 No.2
Purpose: To assess clinical factors and volumetric parameters associated with clinically significant symptomatic radiation pneumonitis (RP), which requires steroid medication after radiotherapy (RT). Methods: Medical records of 204 irradiated breast cancer patients were reviewed. Percent lung volume (PLV) receiving more than 20 Gy was measured from CT-based treatment plan and was correlated with the central lung distance (CLD) of local and regional fields. PLV was also evaluated as a predictive factor of symptomatic RP, along with other previously reported clinical factors. Results: Average (±standard deviation) actual irradiated lung volume and PLV for breast/chest wall irradiation were 169 (±50.6) cm3 and 14.9 (±3.8)%, respectively. Addition of regional irradiation resulted in increase of 183 (±80.2) cm3 in actual irradiated lung volume and 16.5 (±6.2)% in PLV. The correlation between CLD of the local fields and PLV was significant, with 1 cm of CLD corresponding to approximately 6% of PLV. CLD of the regional field was also significantly associated with PLV: a CLD of 3 cm corresponds to a PLV of approximately 13%; a CLD of 4 cm, approximately 17%; and a CLD of 5 cm, approximately 21%. RP developed in 11 patients (5.4%). There was an increased incidence of RP among patients who underwent local RT vs local and regional RT (2.4% vs 12.1%, p=0.0192). In terms of PLV, total PLV ≥23% was associated with the development of RP (p=0.0467). Previously reported clinical factors failed to show statistically significant association. Conclusion: Correlation between CLD and PLV for local and regional fields was significant on volumetric analysis. Although symptomatic RP requiring steroid medication was a rare complication, regional irradiation increased the incidence of RP, and such relationship can be expressed with a volumetric parameter of PLV.
Effect of Fullerene Tris-adducts on the Photovoltaic Performance of P3HT:Fullerene Ternary Blends
Kang, Hyunbum,Kim, Ki-Hyun,Kang, Tae Eui,Cho, Chul-Hee,Park, Sunhee,Yoon, Sung Cheol,Kim, Bumjoon J. American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.10
<P>Fullerene tris-adducts have the potential of achieving high open-circuit voltages (<I>V</I><SUB>OC</SUB>) in bulk heterojunction (BHJ) polymer solar cells (PSCs), because their lowest unoccupied molecular orbital (LUMO) level is higher than those of fullerene mono- and bis-adducts. However, no successful examples of the use of fullerene tris-adducts as electron acceptors have been reported. Herein, we developed a ternary-blend approach for the use of fullerene tris-adducts to fully exploit the merit of their high LUMO level. The compound <I>o</I>-xylenyl C<SUB>60</SUB> tris-adduct (OXCTA) was used as a ternary acceptor in the model system of poly(3-hexylthiophene) (P3HT) as the electron donor and the two soluble fullerene acceptors of OXCTA and fullerene monoadduct (<I>o</I>-xylenyl C<SUB>60</SUB> monoadduct (OXCMA), phenyl C<SUB>61</SUB>-butyric acid methyl ester (PCBM), or indene-C<SUB>60</SUB> monoadduct (ICMA)). To explore the effect of OXCTA in ternary-blend PSC devices, the photovoltaic behavior of the device was investigated in terms of the weight fraction of OXCTA (<I>W</I><SUB>OXCTA</SUB>). When <I>W</I><SUB>OXCTA</SUB> is small (<0.3), OXCTA can generate a synergistic bridging effect between P3HT and the fullerene monoadduct, leading to simultaneous enhancement in both <I>V</I><SUB>OC</SUB> and short-circuit current (<I>J</I><SUB>SC</SUB>). For example, the ternary PSC devices of P3HT:(OXCMA:OXCTA) with <I>W</I><SUB>OXCTA</SUB> of 0.1 and 0.3 exhibited power-conversion efficiencies (PCEs) of 3.91% and 3.96%, respectively, which were significantly higher than the 3.61% provided by the P3HT:OXCMA device. Interestingly, for <I>W</I><SUB>OXCTA</SUB> > 0.7, both <I>V</I><SUB>OC</SUB> and PCE of the ternary-blend PSCs exhibited nonlinear compositional dependence on <I>W</I><SUB>OXCTA</SUB>. We noted that the nonlinear compositional trend of P3HT:(OXCMA:OXCTA) was significantly different from that of P3HT:(OXCMA:<I>o</I>-xylenyl C<SUB>60</SUB> bis-adduct (OXCBA)) ternary-blend PSC devices. The fundamental reasons for the differences between the photovoltaic trends of the two different ternary-blend systems were investigated systemically by comparing their optical, electrical, and morphological properties.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-10/am400695e/production/images/medium/am-2013-00695e_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am400695e'>ACS Electronic Supporting Info</A></P>
Physiologically based pharmacokinetic (PBPK) modeling of fl urbiprofen in different CYP2C9 genotypes
Sang-Sup Whang,Chang-Keun Cho,Eui Hyun Jung,Pureum Kang,Hye-Jung Park,Yun Jeong Lee,Chang-Ik Choi,Jung-Woo Bae,Hyung Sik Kim,Choon-Gon Jang,Seok-Yong Lee 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.8
The aim of this study was to establish the physiologicallybased pharmacokinetic (PBPK) model of fl urbiprofenrelated to CYP2C9 genetic polymorphism anddescribe the pharmacokinetics of fl urbiprofen in diff erentCYP2C9 genotypes. PK-Sim® software was used for themodel development and validation. A total of 16 clinicalpharmacokinetic data for fl urbiprofen in diff erent CYP2C9genotypes, dose regimens, and age groups were used for thePBPK modeling. Turnover number (k cat ) of CYP2C9 valueswere optimized to capture the observed profi les in diff erentCYP2C9 genotypes. In the simulation, predicted fractionmetabolized by CYP2C9, fraction excreted to urine, bioavailability,and volume of distribution were similar to previouslyreported values. Predicted plasma concentration-timeprofi les in diff erent CYP2C9 genotypes were visually similarto the observed profi les. Predicted AUC inf in CYP2C9*1/*2 ,CYP2C9*1/*3 , and CYP2C9*3/*3 genotypes were 1.44-,2.05-, and 3.67-fold higher than the CYP2C9*1/*1 genotype. The ranges of fold errors for AUC inf , C max , and t 1/2 were 0.84–1.00, 0.61–1.22, and 0.74–0.94 in development and0.59–0.98, 0.52–0.97, and 0.61–1.52 in validation, respectively,which were within the acceptance criterion. Thus, thePBPK model was successfully established and described thepharmacokinetics of fl urbiprofen in diff erent CYP2C9 genotypes,dose regimens, and age groups. The present modelcould guide the decision-making of tailored drug administrationstrategy by predicting the pharmacokinetics of fl urbiprofenin various clinical scenarios.
Identification of Differentially Displayed Genes of a Pseudomonas Resistant Soybean (Glycine max)
Kang, Sang-Gu,Cha, Hyeon-Wook,Chang, Moo-Dng,Park, Eui-Ho The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.5
In Korea, a local soybean (Glycine max) genotype 56l. was found to be strongly resistant to a virulent bacterial strain of a Pseudomonas sp. SN239. Specific genes involved in the resistance of the soybean genotype 561 were identified and the pattern of gene expression against the Pseudomonas infection was analyzed using differential-display reverse transcription PCR (DDRT-PCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open reading frames had unknown functions. Sixteen selected cDNA clones were homologous to known genes of other organisms. Some of the identified cDNAs were pathogenesis-related (PR) genes and PR-like genes. These cDNAs included a putative calmodulin-binding protein; an endo-l,3-1,4-$\bate$-D-glucanase; a $\bate$-1,3-endoglucanase; a $\bate$-1,3-exoglucanase; a phytochelatin synthetase-like gene; a thiol protease; a cycloartenol synthase; and a putative receptor-like serine/threonine protein kinase. Among them, four genes were found to be putative PR genes induced significantly by the Pseudomonas infection. These included a calmodulin-binding protein gene, a $\bate$-1,3-endoglucanase gene, a receptor-like serine/threonine protein kinase gene, and pS321 (unknown function). These results suggest that the differentially expressed genes may mediate the strong resistance of soybean 561 to the strain SN239 of Pseudomonas sp.