A novel 1,3,4‑thiadiazole modified chitosan: synthesis, characterization, antimicrobial activity, and release study from film dressings

Mohamed Ahmad E.,Elgammal Walid E.,Dawaba Aya M.,Ibrahim Ahmed G.,Fouda Amr,Hassan Saber M. 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.4

Herein, two new polymers designated as Cs-EATT and Cs-BATT have been synthesized via linking the chitosan with the synthesized 1,3,4-thiadiazole compounds. They were characterized using 1 H, 13 C-NMR, FT-IR, TGA, Elemental analysis, Mass spectrum, and UV–vis spectrophotometer. The synthesized polymers exhibit high activity to control the growth of pathogenic bacteria ( S. aureus , B. subtilis , E. coli, and P. aeruginosa ), and unicellular fungi ( C. albicans ). The MIC values were in the range of 25–100 µg mL –1 for Cs-EATT and 25–200 µg mL –1 for Cs-BATT with varied clear zones. The new polymers were mixed with three film-forming agents: polyvinyl alcohol, hydroxyethyl cellulose, and carboxymethyl cellulose to form six film dressings designated as E1, E2, and E3 for Cs-EATT, and B1, B2, and B3 for Cs-BATT, respectively. The evaluation of film dressings showed that the formed films had transparency, uniformity, homogeneity, elasticity, and non-irritation pH values for skin within the normal range. The maximum percentages of Cs-E/B-ATT content were recorded for film dressings E2 and B2, with values of 92.5% and 94.9%, respectively. Also, the release percentages varied according to film dressing formulation, with values in the ranges of 83.88–93.2% for Cs-EATT and (87.7–97.35%) for Cs-EATT after 9 h.

Direct Antimicrobial Activity and Induction of Systemic Resistance in Potato Plants Against Bacterial Wilt Disease by Plant Extracts

M. A. E. Hassan,M. F. F. Bereika,H. I. G. Abo-Elnaga,M. A. A. Sallam 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4

The potential of three plants extracts, to protect potato plants against bacterial wilt caused by Ralstonia solanacearum was determined under greenhouse and field conditions. All soil drenching treatments of aqueous plant extracts of Hibsicus sabdariffa, Punica granatum and Eucalyptus globulus significantly reduced the disease severity compared with inoculated control. Although the applications of all three plant extracts resulted in similar reductions of disease severity in field up 63.23 to 68.39%, treatment of E. globulus leaf extract was found greater in restricting the symptom development than other the two plant extracts in the greenhouse. More than 94% reduction in the bacterial wilt symptom was observed in potato plants. All tested plant extracts were effective in inhibiting the growth of bacterial pathogen, not only in vitro, but also in stem of potato plants as compared with the inoculated control. Potato plants treated with extract of H. sabdariffa reduced bacterial growth more effectively than treatment with P. granatum and E. globulus. Activity of defence-related enzymes, including peroxidase, polyphenoloxidase and phenylalanine ammonia lyase, were significantly increased in plants treated with the plant extracts compared to the control during the experimental period. In general, the higher enzymes activities were determined in both inoculated and non-inoculated treated potato plants after 8 days from plant extracts treatment. These results suggested that these plant extracts may be play an important role in controlling the potato bacterial wilt disease, through they have antimicrobial activity and induction of systemic resistance in potato plants.

Direct Antimicrobial Activity and Induction of Systemic Resistance in Potato Plants Against Bacterial Wilt Disease by Plant Extracts

Hassan, M.A.E.,Bereika, M.F.F.,Abo-Elnaga, H.I.G.,Sallam, M.A.A. The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4

The potential of three plants extracts, to protect potato plants against bacterial wilt caused by Ralstonia solanacearum was determined under greenhouse and field conditions. All soil drenching treatments of aqueous plant extracts of Hibsicus sabdariffa, Punica granatum and Eucalyptus globulus significantly reduced the disease severity compared with inoculated control. Although the applications of all three plant extracts resulted in similar reductions of disease severity in field up 63.23 to 68.39%, treatment of E. globulus leaf extract was found greater in restricting the symptom development than other the two plant extracts in the greenhouse. More than 94% reduction in the bacterial wilt symptom was observed in potato plants. All tested plant extracts were effective in inhibiting the growth of bacterial pathogen, not only in vitro, but also in stem of potato plants as compared with the inoculated control Potato plants treated with extract of H. sabdariffa reduced bacterial growth more effectively than treatment with P. granatum and E. globulus. Activity of defence-related enzymes, including peroxidase, polyphenoloxidase and phenylalanine ammonia lyase, were significantly increased in plants treated with the plant extracts compared to the control during the experimental period. In general, the higher enzymes activities were determined in both inoculated and non-inoculated treated potato plants after 8 days from plant extracts treatment. These results suggested that these plant extracts may be play an important role in controlling the potato bacterial wilt disease, through they have antimicrobial activity and induction of systemic resistance in potato plants.

Effects of probiotic, prebiotic, and synbiotic with and without feed restriction on performance, hematological indices and carcass characteristics of broiler chickens

Hassan M. Abdel-Hafeez,Elham S.E. Saleh,Samar S. Tawfeek,Ibrahim M.I. Youssef,Asmaa S.A. Abdel-Daim 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.5

Objective: This study was conducted to investigate the effects of probiotic, prebiotic and synbiotic with and without feed restriction on broilers performance, blood parameters, carcass characteristics, and feed cost of production from 1 to 56 days of age. Methods: Two hundred and forty unsexed one day-old chicks of Arbor Acres breed were used. Two trials, I and II, were conducted, with 120 birds in both. Each trial was divided into 4 equal groups. The birds in trial I were fed ad libitum throughout the experiment, while the chicks in trial II were fed ad libitum during the first week of age, then subjected to 5 hours/d of feed restriction from the beginning of the second week up to the end of the experiment. In both trials, the birds in group 1 were fed on a control diet while the other groups were given the same control diet supplemented either with a probiotic in group 2, prebiotic in group 3, or synbiotic in group 4. Results: It was found that chicks fed diets supplemented with probiotic, prebiotic and synbiotic (with and without feed restriction) exhibited higher body weight and feed efficiency than chicks fed the control diets. The feed additives in both trials did not affect hemoglobin, serum total protein, albumin, globulin, glucose, and total cholesterol, except the packed cell volume which was increased in the additive treatments with restriction at the end of the experiment. Moreover, the dietary treatments did not influence the carcass yield. However, the relative weights of liver, gizzard and proventriculus, small intestine and bursa of fabricius were found to be increased. The additives decreased the visible fat in the carcass, with more decreasing effect in the additive groups with restriction. The lowest feed cost per kg of weight gain was observed in the birds fed diets supplemented with synbiotic, probiotic and prebiotic. Feed restriction improved the feed conversion ratio, economic return, but decreased the feed intake, serum total cholesterol and visible fat in comparison with non-restricted groups. Conclusion: The biological feed additives could be routinely added to broiler diets, especially when a feed restriction program is followed. Finally, it can be recommended to restrict feed, and add probiotic or synbiotic to increase weight, improve feed conversion rate and reduce feed cost of production.

On testing NBUL aging class of life distribution

Hassan, M.Kh.,El-Din, M.M. Mohie,Abu-Youssef, S.E. The Korean Reliability Society 2014 International Journal of Reliability and Applicati Vol.15 No.1

Let X and $X_t$ denote the lifetime and the residual life at age t, respectively. X is said to be a NBUL (new better than used in Laplace transform order) random variable if $X_t$ is smaller than X in Laplace order, i.e., $X_t{\leq}_{LT}X$. We propose a new test statistics for testing exponentiality versus NBUL class of life distribution. The tests by Hollender and Proschan (1975) and the generalized Hollender and Proschan test by Ains and Mitra (2011) are considered as special cases of the our of test statistics. Our proposed test statistics is simple, consistent and asymptotically normal. Efficiency and powers of the test statistics for some commonly used distributions in reliability are discussed. Finally, real examples are presented to illustrate the theoretical results.

Immobilization of [Bmim+Tf2NS] hydrophobic ionic liquid on nano-silica-amine sorbent for implementation in solid phase extraction and removal of lead

Hassan M. Al-bishri,Tarek M. Abdel-Fattah,Mohamed E. Mahmoud 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4

A method is described for highly efficient adsorptive removal of lead with a maximum metal capacity value of 1.300 mmol g-1 by using physically immobilized [Bmim+Tf2NS] on the surface of nano-silicaamine sorbent. Lead sorption was found to be highly dependent and controlled by several experimental factors. The effect of sorbent dose played a significant role by yielding the maximum lead adsorption capacity when 100 mg sorbent was used. The effect of lead concentration was examined by various adsorption isotherms. The potential applications for removal of Pb(II) were studied and the percentage recovery values were 99.0–100.0 ± 2.0–5.0%.

On testing NBUL aging class of life distribution

M. KH. Hassan,M. M. Mohie El-Din,S. E. Abu-Youssef 한국신뢰성학회 2014 International Journal of Reliability and Applicati Vol.15 No.1

Let X and Xt denote the lifetime and the residual life at age t , respectively. X is said to be a NBUL (new better than used in Laplace transform order) random variable if Xt is smaller than X in Laplace order, i.e., Xt≤LT X. We propose a new test statistics for testing exponentiality versus NBUL class of life distribution. The tests by Hollender and Proschan (1975) and the generalized Hollender and Proschan test by Ains and Mitra (2011) are considered as special cases of the our of test statistics. Our proposed test statistics is simple, consistent and asymptotically normal. Efficiency and powers of the test statistics for some commonly used distributions in reliability are discussed. Finally, real examples are presented to illustrate the theoretical results.

수생균의 분비물질에 의한 Chlorella fusca 의 성장 및 대사조절

Hassan, S. K. M.,Allah, E. M. Fadl,Kobbia, I. A.,Shoulkamy, M. A. 한국균학회 1990 韓國菌學會誌 Vol.18 No.4

The growth and biochemical activities of Chlorella fusca were studied in the presence of different concentrations of either filtrates or mycelial mats of Saprolegnia ferax and Pythium graminicola. Low concentrations of both fungal filtrates exerted increase in total count, dry weight and in the biosynthesis of photosynthetic pigments, carbohydrates and nitrogen content. High concentrations showed inhibitory effect on both growth and biochemical activities of Chlorella fuscu. Supplementation with different concentrations of dry mycelial mats of either fungi the culture of Chlorella showed elevation in biomass, dry weight, and biosynthesis of carbohydrates and nitrogen content especially at low concentrations. The contents of photosynthetic pigment were inhibited only at low concentrations. Neither the culture filtrate of Pythium nor Saprolegnia had cellulolytic activity, although polygalacturonase enzymes were detected, whereas chloroform-extract of both fungal filtrates showed blue spots under long wave light (366 ㎚).

Electricity generation from rice straw using a microbial fuel cell

Hassan, S.H.A.,Gad El-Rab, S.M.F.,Rahimnejad, M.,Ghasemi, M.,Joo, J.H.,Sik-Ok, Y.,Kim, I.S.,Oh, S.E. Pergamon Press ; Elsevier Science Ltd 2014 International journal of hydrogen energy Vol.39 No.17

This study demonstrated electricity generation from rice straw without pretreatment in a two-chambered microbial fuel cell (MFC) inoculated with a mixed culture of cellulose-degrading bacteria (CDB). The power density reached 145 mW/m<SUP>2</SUP> with an initial rice straw concentration of 1 g/L; while the coulombic efficiencies (CEs) ranged from 54.3 to 45.3%, corresponding to initial rice straw concentrations of 0.5-1 g/L. Stackable MFCs in series and parallel produced an open circuit voltage of 2.17 and 0.723 V, respectively, using hexacyanoferrate as the catholyte. The maximum power for serial connection of three stacked MFCs was 490 mW/m<SUP>2</SUP> (0.5 mA). In parallelly stacked MFCs, the current levels were approximately 3-fold (1.5 mA) higher than those produced from the serial connection. These results demonstrated that electricity can be produced from rice straw by exploiting CDB as the biocatalyst. Thus, this method provides a promising way to utilize rice straw for bioenergy production.

A Novel Algorithm for Edge Detection of Noisy Medical Images

A. E. A. Elaraby,El-Owny,Hassan Badry M. Ahmed,M. Heshmat,M. Hassaballah,A. S. Abel Rardy 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6