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Expression Analysis of the Mx Gene and Its Genome Structure in Chickens
Yin, C.G.,Du, L.X.,Li, S.G.,Zhao, G.P.,Zhang, J.,Wei, C.H.,Xu, L.Y.,Liu, T.,Li, H.B. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.7
Among the known interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful pathways. The Mx protein has direct antiviral activity and inhibits a wide range of viruses by blocking an early stage of the viral replication cycle. Cloning, characterization, and expression of Mx in vivo and in vitro have been conducted. The chicken Mx gene spans 21 kb and is made up of 14 exons and 13 introns, of which the promoter region was analyzed. The real-time PCR results showed that Mx expression was increased in chicken embryo fibroblasts (CEF) after 12- and 24-h induction with polyI: C. Induction of Mx expression by poly I: C in vivo revealed tissue-specific patterns among the chicken tissues tested. A trace expression of Mx was detected in healthy chicken liver tissues from adult chickens without inducement; the expression levels in the liver, heart, and gizzard were higher than in the muscle and kidney. This is the first report to demonstrate the expression of a glutathione-S-transferase-tagged-Mx fusion protein of 75 KDa, as well as the biological activity tested by SDS-PAGE and western blotting.
Du B. C.,Tang G. Z.,Zhang L.,Zhong L.,Deng T.,Yi Y. L. 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11
In view of the problem that local temperature of the original cylinder head is on the high side, an innovative cooling air diversion channel is proposed. The cooling air diversion channel introduces cool air into the cylinder head to forcedly cool the cylinder head. Furthermore, the multiple guide plates are set to reasonably distribute cold air in the air diversion channel. In addition, appropriately increase the length of the fins to cooling the whole cylinder head. The fluid-solid coupling three-dimensional simulation technology is adopted to analyze the impact of the new air diversion channel on the heat dissipation of cylinder head. Finally, the optimal scheme is defined to fabricate the prototype. Simulations results show that the above improvements make the temperature in the high temperature region effectively decrease, and the cooling performance of whole cylinder head is enhanced. In the optimal improvement scheme, the highest temperature of cylinder head decrease by 23 K. Furthermore, the prototype was fabricated according to the optimal scheme. The experimental results show that the temperature of the improved cylinder head decreased effectively, and the highest temperature decreased by 20.4 K. The simulation results are in agreement with the experimental results, and the maximum error is less than 5 percent.
박병준,정세용,이정필,박병철,김철희,한상철,두승규,성태현,한영희,Park, B.J.,Jung, S.Y.,Lee, J.P.,Park, B.C.,Kim, C.H.,Han, S.C.,Du, S.G.,Sung, T.H.,Han, Y.H. 한국초전도학회 2009 Progress in superconductivity Vol.11 No.1
A superconductor flywheel energy storage(SFES) system is mainly act an electro-mechanical battery which transfers mechanical energy into electrical form and vice versa. SFES system consists of a pair of non-contacting High Temperature Superconductor (HTS) bearings with a very low frictional loss. But it is essential to design an efficient HTS bearing considering with rotor dynamic properties through correct calculation of stiffness in order to support a huge composite flywheel rotor with high energy storage density. Static properties of HTS bearings provide data to solve problems which may occur easily in a running system. Since stiffness to counter vibration is the main parameter in designing an HTS bearing system, we investigate HTS bearing magnetic force through static properties between the Permanent Magnet(PM) and HTS. We measured axial / radial stiffness and found bearing stiffness can be easily changed by activated vibration direction between PM and HTS bulk. These results are used to determine the optimal design for a 10 kWh SFES.
Du, H.I.,Han, B.S.,Kim, Y.J.,Lee, D.H.,Song, S.S.,Han, T.H.,Han, S.C. North-Holland 2011 Physica. C, Superconductivity Vol.471 No.21
The basic way to improve the performance of a superconducting current limiter is to apply and evaluate a superconducting device that is appropriate to the superconducting current limiter. Among the many types of superconducting devices, the YBCO thin film wire has excellent current-limiting performance that is appropriate for actual system application. For the application of the YBCO thin film wire to superconducting current limiters, its current-limiting performance as a unit device must be accurately evaluated, and measures to improve its current-limiting performance must be sought. Accordingly, to evaluate the current-limiting performance of the YBCO thin film wire, this study was conducted to evaluate its resistance-increasing trend, V<SUB>max</SUB>, T<SUB>r</SUB>, I<SUB>max</SUB>, I<SUB>qt</SUB>, and current-limiting rate as a unit device, after which the electric coupling condition that consists of a core and windings was used to evaluate the current-limiting performance of the YBCO thin film wire.
Yim, S.W.,Park, B.C.,Jeong, Y.T.,Kim, Y.J.,Yang, S.E.,Kim, W.S.,Kim, H.R.,Du, H.I. North-Holland 2013 Physica. C, Superconductivity Vol.484 No.-
A 22.9kV class hybrid fault current limiter (FCL) developed by Korea Electric Power Corporation and LS Industrial Systems in 2006 operates using the line commutation mechanism and begins to limit the fault current after the first half-cycle. The first peak of the fault current is available for protective coordination in the power system. However, it also produces a large electromagnetic force and imposes a huge stress on power facilities such as the main transformer and gas-insulated switchgear. In this study, we improved the operational characteristics of the hybrid FCL in order to reduce the first peak of the fault current. While maintaining the structure of the hybrid FCL system, we developed a superconducting module that detects and limits the fault current during the first half-cycle. To maintain the protective coordination capacity, the hybrid FCL was designed to reduce the first peak value of the fault current by up to approximately 30%. The superconducting module was also designed to produce a minimum AC loss, generating a small, uniform magnetic field distribution during normal operation. Performance tests confirmed that when applied to the hybrid FCL, the superconducting module showed successful current limiting operation without any damage.
Zeng, X.C.,Chen, H.Y.,Hui, W.Q.,Jia, B.,Du, Y.C.,Tian, Y.Z. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.12
The aim of this study was to evaluate, through the use of microsatellite markers, the current genetic diversity and the relationships of 375 individuals from 8 local sheep breeds reared in typical breeding farms in the northwest of China, and moreover, to offer a contribution towards genetic conservation decisions for the studied breeds. The expected heterozygosities and allelic richness for the 8 breeds varied from 0.474 to 0.623 and from 3.8 to 5.4, respectively. All the populations showed a significant deficit in heterozygosity and a relatively low level of genetic diversity. Furthermore, the high positive FIS value (ranging from 0.255 to 0.556) indicated inbreeding to be one of the main causes for high genetic homogeneity and lack of heterozygosity in all breeds. The clustering analysis performed with the DISPAN package showed that Aletai, Kazak, Bashibai and Bayinbuluke were grouped together, and Hetian, Qira black and Duolang were grouped together, which indicated that the relationship among breeds displayed some degree of consistency with their geographical distribution, production and origin. These findings indicate that improved conservation measures must be undertaken to avoid further losses of genetic diversity and minimize inbreeding represented by these breeds.
Das, M.,Du, Y.,Mortensen, J.,Ribeiro, O.,Hariharan, P.,Guan, L.,Loland, C.,Kobilka, B.,Byrne, B.,Chae, P. THE ROYAL SOCIETY OF CHEMISTRY 2017 Chemical Science Vol.8 No.2
<P>Amphiphile selection is a crucial step in membrane protein structural and functional study. As conventional detergents have limited scope and utility, novel agents with enhanced efficacy need to be developed. Although a large number of novel agents have been reported, so far there has been no systematically designed comparative study of the protein stabilization efficacy of stereo-isomeric amphiphiles. Here we designed and prepared a novel class of stereo-isomeric amphiphiles, designated butane-1,2,3,4-tetraolbased maltosides (BTMs). These stereoisomers showed markedly different behaviour for most of the targeted membrane proteins depending on the chirality of the linker region. These findings indicate an important role for detergent stereochemistry in membrane protein stabilization. In addition, we generally observed enhanced detergent efficacy with increasing alkyl chain length, reinforcing the importance of the balance between hydrophobicity and hydrophilicity in detergent design. The stereo-isomeric difference in detergent efficacy observed provides an important design principle for the development of novel amphiphiles for membrane protein manipulation.</P>