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Yong-Ping Chen,Feng-Bin Lu,Da-Zhi Chen,Lu Chen,En-De Hu,Jin-Lu Wu,Hui Li,Yue-Wen Gong,Zhuo Lin,Xiao-Dong Wang,Ji Li,Xiao-Ya Jin,Lan-Man Xu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12
MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.
Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126
Chen, Lan,Yang, Yang,Han, Jun,Zhang, Bao-Yun,Zhao, Lin,Nie, Kai,Wang, Xiao-Fan,Li, Feng,Gao, Chen,Dong, Xiao-Ping,Xu, Cai-Min Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
Although the function of cellular prion protein (PrP$^C$) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.
Modified embedded-atom interatomic potential for Co-W and Al-W systems
DONG, Wei-ping,CHEN, Zheng,LEE, Byeong-Joo Elsevier 2015 Transactions of Nonferrous Metals Society of China Vol.25 No.3
<P>A semi-empirical interatomic potential formalism, the second-nearest-neighbor modified embedded-atom method (2NN MEAM), has been applied to obtaining interatomic potentials for the Co-W and Al-W binary system using previously developed MEAM potentials of Co, Al and W. The potential parameters were determined by fitting the experimental data on the enthalpy of formation, lattice parameter, melting point and elastic constants. The present potentials generally reproduce the fundamental physical properties of the Co-W and Al-W systems accurately. The lattice parameters, the enthalpy of formation, the thermal stability and the elastic constants match well with experiment and the first-principles results. The enthalpy of mixing and the enthalpy of formation and mixing of liquid are in good agreement with CALPHAD calculations. The potentials can be easily combined with already-developed MEAM potentials for binary cobalt systems and can be used to describe Co-Al-W-based multicomponent alloys, especially for interfacial properties.</P>
Dong-Feng Chen,Xican Li,Zhiwei Xu,Xiaobing Liu,Shao-Hui Du,Hui Li,Jian-Hong Zhou,He-Ping Zeng,Zi-Chun Hua 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4
Buzhong Yiqi decoction (BYD) is a well-known ancient tonic prescription in traditional Chinese medicine (TCM). The purpose of this study is to identify active components of BYD involved in promoting proliferation of mesenchymal stem cells (MSCs) and to investigate its mechanism. BYD was extracted with petroleum ether, ethanol, and water. Evidence provided by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, bromodeoxyuridine, proliferation cell nuclear antigen immunoreactivity, cell cycle analysis, and gas chromatography-mass spectrometry indicated that hexadecanoic acid (HA) in BYD extracted with petroleum ether is the active compound responsible for increasing proliferation of MSCs. Western blot analysis show that HA significantly increase retinoic acid receptor (RAR) levels of MSCs, but not estrogen receptor, thyroid hormone receptor, vitamin D receptor, glucocorticoid receptor, and peroxisome proliferator-activated receptor. Reverse transcription-polymerase chain reaction revealed that HA significantly increased RAR mRNA levels. Furthermore, the mechanism of HA action depends on RAR pathway and up-regulates expression of mRNA for insulin-like growth factor-I, the target gene of RAR. Our findings have now allowed for a refinement in our understanding of TCM with respect to pharmacological regulation of stem cells and may be useful to stem cell biology and therapy.
Ping-Rong Yuan,Hyun-Jung Kim,Qiong-Hua Chen,Hong-Guang Ju,Seung-Joon Lee,Shi-Dong Ji,안상낙 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4
The nearly isogenic nature of the introgression lines (ILs) provides a relative advantage over other segregating populations in therapid implementation of pyramiding approach through crosses and marker analysis. A set of 126 ILs carrying various introgressedsegments from a presumed wild progenitor, Oryza rufipogon Griff. Acc. W1944 in the background of an elite Korea japonica cultivar(O. sativa L.), Hwayeongbyeo, was constructed using the marker assisted selection (MAS) technique combined with repeatedbackcrosses. The 126 ILs have different W1944 segments on each chromosome, with 100% coverage of wild segments on chromosome1, while for chromosome 10, the coverage was only 33.3%. The mean number of homozygous and heterozygous donor segmentswere 3 (ranging from 0 to 7) and 4.7 (ranging from 1 to 14.5), respectively, and 31.6% of introgressed segments had sizes ofless than 10.5 cM. A total of 41 quantitative trait loci (QTLs) and two loci associated with pericarp coloration were identified. Thenumber of QTLs per trait ranged from 2 to 6. Phenotypic variance associated with each QTL varied from 9.1 to 52.2%, with an averageof 17.1%. For 11 (26.8%) of the QTLs detected in this study, the O. rufipogon-derived alleles contributed the desired agronomiceffect despite the overall inferior characteristics of the wild phenotype. Favorable alleles from the O. rufipogon accession were identifiedfor panicle number, panicle length, days to heading, secondary branches, spikelets per panicle, and 1,000-grain weight.
( Ping Lu ),( Ke Jiang ),( Ya-qiao Hao ),( Wan-ying Chu ),( Yu-dong Xu ),( Jia-yao Yang ),( Jia-le Chen ),( Guo-hong Zeng ),( Zhou-hang Gu ),( Hong-xin Zhao ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9
Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.
Absence of EZH2 Gene Mutation in Chronic Myeloid Leukemia Patients in Blast Crisis
Chen, Hao-Yue,Yao, Hong,Wu, Ling-Yu,Liu, Can-Jun,Zhu, Jian-Qin,Liu, Chun-Hua,Wang, Wei,Dong, Sha-Sha,Ping, Na-Na,Chen, Su-Ning,Sun, Miao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
First Resolution of Microlensed Images
Dong, Subo,Mé,rand, A.,Delplancke-Strö,bele, F.,Gould, Andrew,Chen, Ping,Post, R.,Kochanek, C. S.,Stanek, K. Z.,Christie, G. W.,Mutel, Robert,Natusch, T.,Holoien, T. W.-S.,Prieto, J. L.,Shap American Astronomical Society 2019 The Astrophysical journal Vol.871 No.1
Ping-Rong Yuan,Shi-Dong Ji,안상낙,김현정,Qiong-Hua Chen,Hong-Guang Ju 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4
Quantitative trait loci (QTL) associated with four milling recovery properties, two chemical properties, six paste properties of grain, and six textural parameters of cooked rice were identified using an introgression line (IL) population of rice developed from an interspecific cross over two years. The IL population consisted of 121 lines from a cross between wild rice (O. rufipogon Griff.) and a japonica cultivar. A total of 28 QTLs were identified for the 14 traits using inter mapping. Of these, 10 were significant over two years indicating that these QTLs are stable across years and environments. For eight (21%) of the QTLs identified, the O. rufipogonderived alleles contributed a desirable effect on amylose content, protein content, minimum viscosity, final viscosity, and consistency. Among these, pc3 for protein content and ac7 for amylose content were significant in both years and showed an R² value of 25.5and 30.9%, respectively. The markers closely associated with these useful alleles can be used to trace the inheritance of specific chromosome segments in the IL population and also offer a starting point for map-based cloning of genes underlying these traits.
Chen Hao,Zhang Dong-Ming,Zhang Zhi-Ping,Li Ming-Zhang,Wu Hai-Feng 한국유전학회 2021 Genes & Genomics Vol.43 No.12
Background Mitochondrial unfolded protein response plays an important role in the occurrence and development of breast cancer. However, the role of mitochondrial unfolded protein response (UPRmt) in the sensitivity of breast cancer to cisplatin chemotherapy has not yet been cleared. Objectives The purpose of this study is to explore the role of mitochondrial unfolded protein response in breast cancer sensitivity to cisplatin. Methods In this study, qRT-PCR, Western blotting, Immunofuorescence, CCK-8, Colony formation, Transwell assay and TUNEL staining assay were used to confrm the role of UPRmt in breast cancer cells treated with cisplatin. Results Cisplatin increased the levels of UPRmt including CLPP, HSP60, LONP1 in MCF7 and MDA-MB-231 cells. UPRmt inducer Nicotinamide ribose (NR) could promote the proliferation and invasion of breast cancer cells treated with cisplatin. Importantly, SIRT3 was discovered to increase UPRmt in breast cancer cells and silencing of SIRT3 could inhibit the efect of NR in breast cancer. Conclusions UPRmt regulated by SIRT3 could protect breast cancer cell from cisplatin. Controlling SIRT3-induced UPR may be a potential therapeutic target to increase the sensitivity of breast cancer chemotherapy.