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Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes
Dong, Xiaohu,Han, Ji Hee,Heo, Cheol Ho,Kim, Hwan Myung,Liu, Zhihong,Cho, Bong Rae American Chemical Society 2012 ANALYTICAL CHEMISTRY - Vol.84 No.19
<P>We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg<SUP>2+</SUP> ([Mg<SUP>2+</SUP>]<SUB>i</SUB>) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca<SUP>2+</SUP> ([Ca<SUP>2+</SUP>]<SUB>m</SUB>), FMg2 allows dual-color imaging of Mg<SUP>2+</SUP>/Ca<SUP>2+</SUP> activities in live cells and [Mg<SUP>2+</SUP>]<SUB>i</SUB> /[Ca<SUP>2+</SUP>]<SUB>m</SUB> distributions in live tissues at a depth of 100–200 μm.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2012/ancham.2012.84.issue-19/ac302210v/production/images/medium/ac-2012-02210v_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac302210v'>ACS Electronic Supporting Info</A></P>
Quinoline-Based Two-Photon Fluorescent Probe for Nitric Oxide in Live Cells and Tissues
Dong, Xiaohu,Heo, Cheol Ho,Chen, Shiyu,Kim, Hwan Myung,Liu, Zhihong American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.1
<P>A two-photon fluorescent probe (QNO) for nitric oxide is reported. The probe is designed with a photoinduced electron transfer (PeT) mechanism and shows 12-fold fluorescence enhancement toward NO. Adopting a quinoline derivative as the fluorophore, QNO has a large two-photon action cross section value of 52 GM and long-wavelength emission. It also features high selectivity, low cytotoxicity, and pH insensitivity. By utilizing two-photon microscopy (TPM), QNO can detect NO in live cells and live tissues at a depth of 90-180 mu m.</P>
Minghan. Dong,Hao. Ma,Zhihong. Bai 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
Dynamic currents and voltages in power electronic converters often exceed rating when changing load. In this way, this paper researches on optimizing transient performance of LCL-type gird-connected inverter systems. Resistor connected in series with capacitor as passive damping (RCSCPD), which is widely used to damp resonance of LCL filter, needs to choose an appropriate resistor to acquire accurate and fast transient performance. To meet this requirement, an approach is proposed for analyzing and improving transient performance. It shows that passive resistor could damp resonance and stable system. Limitation of RCSCPD, which could not optimize dominant transient response, is exhibited as well. Hence, mix damping (MD) is proposed to achieve the goal. It could eliminate overshoot and decrease settling time efficiently when comparing with RCSCPD. MD could configure the poles effectively without adding precision sensors because it uses grid current, which used in control loop, to realize active damping resistor. Experimental results based on single-phase LCL-type gird-connected inverter prototype are given to show optimizing effects of transient performance.
Boundary layer effect of light transmission through ionic liquid under electric field
Zhou Ji,Zheng Yi,Dong Shikui,He Zhihong,Liu Bao,Zhang Yanhu 한국물리학회 2023 Current Applied Physics Vol.52 No.-
The field strength distribution in the ionic liquid boundary layer under the action of an external electric field is non-uniform. Whether its transmittance (absorption coefficient) spatially varies arouses our interest in exploring the underlying laws. In this paper, with the help of an infrared microscope, a special experimental device was designed and established. The transmittance in the ionic liquid (methyl propyl imidazole iodide, C3MImI) at different distances from the electrodes (cathode, anode) was measured under electric voltages from 0 V to 0.5 V. Results showed that transmittances of ionic liquid at the boundary layer present nonlinear change with the nonlinear electronic strength under the different applied voltage, and there is a critical threshold (0.2 V) distinguishes the sensitive and non-sensitive responses to the electric field. Our study provides a physical basis for microscale electro-optic regulation and is significant, especially for precise regulation of light field transmittance in micro-nano channels/structures.
Zhongshan Wang,Haiyan Qin,Zhihong Feng,Guofeng Wu,Shizhu Bai,Yan Dong,Yimin Zhao 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
Bacterial invasion and epithelial downgrowth with pocket formation are still severe clinical challenges for transcutaneous implants, and both have a close relationship with the lack of a stable biological seal around the transcutaneous parts. Dermal fibroblasts are the main cells in the skin tissue and have been proven to play vital roles in the formation of biological seals. In this work, platelet-rich plasma (PRP), which can release high concentrations of natural cytokines upon activation, was used to stimulate rapid fibroblast growth. Mussel adhesive proteins (MAPs) were used as mediators to anchor the platelets in the PRP onto Ti surfaces, and MAP/PRP composite-coated Ti surfaces were constructed successfully. This in vitro study indicated increased fibroblast adhesion (P < 0.05), spreading, and proliferation (P < 0.05) and upregulated extracellular matrix-related gene expression (P < 0.05) on a MAP/PRP composite-coated Ti surface compared with a control smooth Ti surface. Our results suggest that MAP/PRP composite-coated Ti surfaces are potentially useful for the formation of a stable biological seal in transcutaneous areas.
Ultrafast epitaxial growth of metre-sized single-crystal graphene on industrial Cu foil
Xu, Xiaozhi,Zhang, Zhihong,Dong, Jichen,Yi, Ding,Niu, Jingjing,Wu, Muhong,Lin, Li,Yin, Rongkang,Li, Mingqiang,Zhou, Jingyuan,Wang, Shaoxin,Sun, Junliang,Duan, Xiaojie,Gao, Peng,Jiang, Ying,Wu, Xiaoson Elsevier 2017 Science bulletin Vol.62 No.15
Zhang Fei,Peng Wuxun,Wang Tao,Zhang Jian,Dong Wentao,Wang Chuan,Xie Zhihong,Luo Hong,Liu Gang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Bone marrow mesenchymal stem cells (BMSCs) have been used in the treatment of early steroid-induced osteonecrosis of the femoral head (SONFH). However, the hypoxic microenvironment in the osteonecrotic area leads to hypoxia-induced apoptosis of transplanted BMSCs, which limits their efficacy. Therefore, approaches that inhibit hypoxia-induced apoptosis of BMSCs are promising for augmenting the efficacy of BMSC transplantation. Our present study found that under hypoxia, the expression of the long noncoding RNA (Lnc) transmembrane protein 235 (Tmem235) was downregulated, the expression of Bcl-2-associated X protein was upregulated, the expression of B-cell lymphoma-2 protein was downregulated, and the apoptotic rate of BMSCs was over 70%. However, overexpression of Lnc Tmem235 reversed hypoxia-induced apoptosis of BMSCs and promoted their survival. These results demonstrated that Lnc Tmem235 effectively inhibited hypoxia-induced apoptosis of BMSCs. Mechanistically, we found that Lnc Tmem235 exhibited competitive binding to miR-34a-3p compared with BIRC5 mRNA, which is an inhibitor of apoptosis; this competitive binding relieved the silencing effect of miR-34a-3p on BIRC5 mRNA to ultimately inhibit hypoxia-induced apoptosis of BMSCs by promoting the expression of BIRC5. Furthermore, we cocultured BMSCs overexpressing Lnc Tmem235 with xenogeneic antigen-extracted cancellous bone to construct tissue-engineered bone to repair a model of early SONFH in vivo. The results showed that overexpression of Lnc Tmem235 effectively reduced apoptosis of BMSCs in the hypoxic microenvironment of osteonecrosis and improved the effect of BMSC transplantation. Taken together, our findings show that Lnc Tmem235 inhibited hypoxia-induced apoptosis of BMSCs by regulating the miR-34a-3p/BIRC5 axis, thus improving the transplantation efficacy of BMSCs for treating early SONFH.
Guo Mengpei,Ma Xiaolong,Zhou Yan,Bian Yinbing,Liu Gaolei,Cai Yingli,Huang Tianji,Dong Hongxia,Cai Dingjun,Wan Xueji,Wang Zhihong,Xiao Yang,Kang Heng 한국미생물학회 2023 The journal of microbiology Vol.61 No.1
The basidiomycetous edible mushroom Stropharia rugosoannulata has excellent nutrition, medicine, bioremediation, and biocontrol properties. S. rugosoannulata has been widely and easily cultivated using agricultural by-products showing strong lignocellulose degradation capacity. However, the unavailable high-quality genome information has hindered the research on gene function and molecular breeding of S. rugosoannulata. This study provided a high-quality genome assembly and annotation from S. rugosoannulata monokaryotic strain QGU27 based on combined Illumina-Nanopore data. The genome size was about 47.97 Mb and consisted of 20 scaffolds, with an N50 of 3.73 Mb and a GC content of 47.9%. The repetitive sequences accounted for 17.41% of the genome, mostly long terminal repeats (LTRs). A total of 15,726 coding gene sequences were putatively identified with the BUSCO score of 98.7%. There are 142 genes encoding plant cell wall degrading enzymes (PCWDEs) in the genome, and 52, 39, 30, 11, 8, and 2 genes related to lignin, cellulose, hemicellulose, pectin, chitin, and cutin degradation, respectively. Comparative genomic analysis revealed that S. rugosoannulata is superior in utilizing aldehyde-containing lignins and is possible to utilize algae during the cultivation.
Silicate Ions Derived from Calcium Silicate Extract Decelerate Ang II-Induced Cardiac Remodeling
Li Xin,Zhang Yanxin,Jin Qishu,Song Qiaoyu,Fan Chen,Jiao Yiren,Yang Chen,Chang Jiang,Dong Zhihong,Que Yumei 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5
BACKGROUND: Pathological cardiac hypertrophy is one of the main activators of heart failure. Currently, no drug can completely reverse or inhibit the development of pathological cardiac hypertrophy. To this end, we proposed a silicate ion therapy based on extract derived from calcium silicate (CS) bioceramics for the treatment of angiotensin II (Ang II) induced cardiac hypertrophy. METHODS: In this study, the Ang II induced cardiac hypertrophy mouse model was established, and the silicate ion extract was injected to mice intravenously. The cardiac function was evaluated by using a high-resolution Vevo 3100 small animal ultrasound imaging system. Wheat germ Agglutinin, Fluo4-AM staining and immunofluorescent staining was conducted to assess the cardiac hypertrophy, intracellular calcium and angiogenesis of heart tissue, respectively. RESULTS: The in vitro results showed that silicate ions could inhibit the cell size of cardiomyocytes, reduce cardiac hypertrophic gene expression, including atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and b-myosin heavy chain (b-MHC), decrease the content of intracellular calcium induced by Ang II. In vivo experiments in mice confirmed that intravenous injection of silicate ions could remarkably inhibit the cardiac hypertrophy and promote the formation of capillaries, further alleviating Ang II-induced cardiac function disorder. CONCLUSION: This study demonstrated that the released silicate ions from CS possessed potential value as a novel therapeutic strategy of pathological cardiac hypertrophy, which provided a new insight for clinical trials.