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Zhao Man,Ju Yanhu,Zhao Bo,Li Xiaoming,Dai LIli,Qu Jicheng,Chu Zhaohui,Ding Xinhua 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5
Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively afecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 diferentially expressed genes were identifed in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifcally up-regulated under drought stress and were functionally enriched in starch and sucrose biosyn�thetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.
Path Smoothing for Five-Axis Machine Tools Using Dual Quaternion Approximation with Dominant Points
Xin Zhao,Huan Zhao,Xiangfei Li,Han Ding 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.5
Nowadays, G01 blocks are still the most widely utilized representation form for the tool paths in the five-axis machine tools. Tangency and curvature discontinuities of the linear tool paths may lead to frequent feedrate fluctuation and acceleration variation, which inevitably deteriorate the machining efficiency and quality. To solve this problem, this paper proposed a dual quaternion B-spline approximation method of dominant points to generate smooth tool paths for five-axis CNC machine tools. First, the dominant points, which can characterize the shape of the linear path, are selected for the tool tip location and the tool orientation simultaneously. Such scheme can significantly compress the number of the control points and release the computational load. Then, the linear tool paths are converted to dual quaternions and approximated to dual quaternion B-spline, which can realize the parameter synchronization for the tool tip and tool orientation, and avoid the fitting oscillatory as well. To verify the proposed method, a trajectory generation method for the dual quaternion B-spline is developed. Simulations and experiments on a five-axis machine tool are conducted, and the results demonstrate the feasibility and efficiency of the proposed method.
Ming Zhao,Ming Zhao,Bin Tong,Zinan Kang,Yanfang Wu,Wei Ding,Baoming Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5
The synthesis of PdP nanoparticles (NPs) using triphenylphosphine as a phosphorus precursor was investigated. The electrocatalytic performance of PdP NPs in hydrogen evolution reaction in an alkaline medium was measured. It can be concluded that when the current density is 10 mA cm -2, the overpotential produced by Pd3P0.95 NPs is 75mV which is 185mV lower than that produced by commercial Pd/C. The mass activity of Pd3P0.95 NPs is more than twice as high as that of commercial Pd/C. The work indicates that PdP nanomaterials could be a potential catalyst for water splitting.
Zhao Liang,Hao Ding,Guangming Wang,Xiaohui Zhang 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.2
In order to explore the optimal spraying effect of diff erent electrostatic spraying parameters on droplet deposition on the back of target, the application height, angle, charging voltage, and nozzle water pressure had been applied as spraying variables for the test designed to investigate on droplet density, deposition and coverage in diff erent depth areas of the target. The results of the study showed that the height and angle of the spatial parameters of the application had a significant effect on the application effect. The droplet deposition effect decreased by 94.71%, 89.09% and 94.17% at diff erent depths on the back of the target as the height increased from 20 to 50 cm. The deposition effects of droplets at diff erent depths on the back of the target at 60° tilt angle are 5.91%, 9.81% and 51.97% of them at 0° tilt angle respectively. The results of orthogonal experiment showed that the optimal parameters of diff erent depth areas on the back of different targets were inconsistent. The height and angle of application are the main influencing factors, when the target is warped up. The depth range of the target (5–7.5 cm) is greatly affected by the nozzle water pressure. In the actual spraying pesticides application, the appropriate operating parameters can be selected according to the size and attitude of the leaf surface to improve the spraying effect.
( Ding Xin Wu ),( Lin Chun Wang ),( Yuwei Li ),( Shu Miao Zhao ),( Nan Peng ),( Yun Xiang Liang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
An exo-β-D-glucosaminidase (AorCsxA) from Aspergillus oryzae FL402 was heterologously expressed and purified. The deduced amino acid sequence indicated that AorCsxA belonged to glycoside hydrolase family 2. AorCsxA digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-D-glucosaminidase (CsxA) activity. AorCsxA exhibited optimal activity at pH 5.5 and 50°C; however, the enzyme expressed Pichia pastoris (PpAorCsxA) showed much stronger thermostability at 50°C than that expressed in Escherichia coli (EcAorCsxA), which may be related to glycosylation. AorCsxA activity was inhibited by EDTA and most of the tested metal ions. A single amino acid mutation (F769W) AorCsxA significantly enhanced the specific activity and hydrolysis velocity as revealed comparison of V_{max} and k_{cat} values with those of the wild-type enzyme. The three-dimensional structure suggested the tightened pocket at the active site of F769W enabled efficient substrate binding. The AorCsxA gene was heterologously expressed in P. pastoris, and one transformant was found to produce 222 U/ml activity during the high-cell-density fermentation. This AorCsxA-overexpressing P. pastoris strain is feasible for large-scale production of AorCsxA.
Structural health monitoring of a high-speed railway bridge: five years review and lessons learned
Ding, Youliang,Ren, Pu,Zhao, Hanwei,Miao, Changqing Techno-Press 2018 Smart Structures and Systems, An International Jou Vol.21 No.5
Based on monitoring data collected from the Nanjing Dashengguan Bridge over the last five years, this paper systematically investigates the effects of temperature field and train loadings on the structural responses of this long-span high-speed railway bridge, and establishes the early warning thresholds for various structural responses. Then, some lessons drawn from the structural health monitoring system of this bridge are summarized. The main context includes: (1) Polynomial regression models are established for monitoring temperature effects on modal frequencies of the main girder and hangers, longitudinal displacements of the bearings, and static strains of the truss members; (2) The correlation between structural vibration accelerations and train speeds is investigated, focusing on the resonance characteristics of the bridge at the specific train speeds; (3) With regard to various static and dynamic responses of the bridge, early warning thresholds are established by using mean control chart analysis and probabilistic analysis; (4) Two lessons are drawn from the experiences in the bridge operation, which involves the lacks of the health monitoring for telescopic devices on the beam-end and bolt fractures in key members of the main truss.
RAMS evaluation for a steel-truss arch high-speed railway bridge based on SHM system
Zhao, Han-Wei,Ding, You-Liang,Geng, Fang-Fang,Li, Ai-Qun Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.1
The evaluation theory of reliability, availability, maintainability and safety (RAMS) as a mature theory of state evaluation in the railway engineering, can be well used to the evaluation, management, and maintenance of complicated structure like the long-span bridge structures on the high-speed railway. Taking a typical steel-truss arch bridge on the Beijing-Shanghai high-speed railway, the Nanjing Dashengguan Yangtze River Bridge, this paper developed a new method of state evaluation for the existing steel-truss arch high-speed railway bridge. The evaluation framework of serving state for the bridge structure is presented based on the RAMS theory. According to the failure-risk, safety/availability, maintenance of bridge members, the state evaluation method of each monitoring item is presented. The weights of the performance items and the monitoring items in all evaluation levels are obtained using the analytic hierarchy process. Finally, the comprehensive serving state of bridge structure is hierarchical evaluated.
Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,Sun, Xue-Fang,Zhao, Ming 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of $CO_2$ in $C_4$ plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a $C_4$ SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed.