Rapid determination of formic and acetic acids in biomass hydrolysate by headspace gas chromatography

Dandan Zhou,Qingxi Hou,Wei Liu,임효례 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-

A headspace gas chromatography (HS-GC) method is developed for determination of formic acid (FA) andacetic acid (AA) in biomass hydrolysate. The method is based on the “in-vial” derivatization reaction ofalcohols and acids. NaHSO4/ethanol is selected as the preferred derivatization reagent. The method has areproducibility of RSD <0.87% and recovery of 97.1%–103.1%. The limits of the quantification were2.02 and 2.38 mg/L for FA and AA, respectively. The GC analysis time only required 12.4 min. The methodis rapid, sensitive and suitable for measuring FA and AA in the multifarious biomass hydrolysates inpulping and biorefinery industries.

Granulation of Activated Sludge in a Continuous Flow Airlift Reactor by Strong Drag Force

Dandan Zhou,Mengyuan Liu,Jun Wang,Shuangshi Dong,Ning Cui,Linlin Gao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

Most aerobic granule cultivation has been based on the sequencing batch reactor (SBR) and then the factors that affect aerobic granulations were developed in the SBR. However, little work has been done to cultivate aerobic granules in a continuous-flow bioreactor with simple structure that is realistic for engineering. This work is the first to cultivate aerobic granules in a continuous flow airlift fluidized bed reactor (CAFB) possesses a very simple structure and without settling time and starvation time controlling. The configuration of CAFB was the simplest continuous-flow aerobic granular bioreactor reported by now. The majority of granules could be formatted in the CAFB after 12 days cultivation. The effluent COD concentration maintained at 50 ± 10 mg/L for the variable COD loading rate of 3.5 g COD/L/d and 4.8 g COD/L/d, which confirmed that the CAFB performed good anti-shock abilities. CAFB performed good nitrification ability, however, little denitrification was found under the operating conditions of this study. The shear stress acting on the solid phase were hundreds of times stronger in the CAFB than in the SBR at the same aeration strength. It seems CAFB is very efficient for granulation due to the strong shear-force exertion,which is promising for continuous-flow aerobic granular bioreactor. Protein, positive to the hydrophobicity, was predominant in extracellular polymeric substances in the granules, and favored the granules formation in the CAFB combined with the polysaccharides. However, filamentous bulking always happened in 35 days operation of the CAFB, thus further study on the stability of this bioreactor is urgently necessary.

Explore the notion of education for sustainable development in early childhood education in China

Dandan Zhou The Pacific Early Childhood Education Research Ass 2021 Asia-Pacific journal of research in early childhoo Vol.15 No.1

Education for sustainable development (ESD) has been a worldwide topic in educational sectors, which aims to create a sustainable world. This paper examines the ways that the notion of ESD emerging from early childhood practices in China. Findings were reported from a qualitative case study, which involved seven participants from two kindergartens in China. Evidence from multiple methods was used to demonstrate teaching practices related to ESD and understand teachers’ responses. The findings revealed that environmental education as an aspect of ESD had been included in the kindergarten curriculum in China, which was conducted through teacher-initiated group activity and daily routines. However, not much attention had been paid to the social dimension and especially the economic dimension. This paper argues that the implementation of ESD in kindergartens in China should involve more dimensions to equip children with the knowledge of sustainable development of the environment, society, and economy.

Lactate potentiates angiogenesis and neurogenesis in experimental intracerebral hemorrhage

Jing Zhou,Tao Liu,Hao Guo,Hanjin Cui,Pengfei Li,Dandan Feng,En Hu,Qing Huang,Ali Yang,Jun Zhou,Jiekun Luo,Tao Tang,Yang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Lactate accumulation has been observed in the brain with intracerebral hemorrhage (ICH). However, the outcome of lactate accumulation has not been well characterized. Here, we report that lactate accumulation contributes to angiogenesis and neurogenesis in ICH. In the first set of the experiment, a rat model of ICH was induced by injecting collagenase into the brain. The effects of lactate accumulation on the neurological function, apoptosis, and numbers of newborn endothelial cells and neurons, as well as the proliferation-associated signaling pathway, were evaluated in the rat brain. In the second set, exogenous L-lactate was infused into intact rat brains so that its effects could be further assessed. Following ICH, lactate accumulated around the hematoma; the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were significantly increased compared with the numbers in the Sham group. Moreover, ICH induced translocation of nuclear factor-kappa B (NF-κB) p65 into the nucleus, resulting in a notable upregulation of VEGF and bFGF mRNAs and proteins compared with the levels in the Sham controls. Administration of a lactate dehydrogenase inhibitor dramatically inhibited these effects, decreased the vascular density, and aggravated neurological severity scores and apoptosis after ICH. After exogenous L-lactate infusion, the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were strikingly increased compared with the numbers in the Sham controls. In addition, lactate facilitated NF-κB translocation to induce increased transcription of VEGF and bFGF. Co-infusion with an NF-κB inhibitor significantly inhibited these effects. These data suggest that lactate potentiates angiogenesis and neurogenesis by activating the NF-κB signaling pathway following ICH.

Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-β/collagen athway in preeclampsia

Xiang-Hong Xu,Yuanhui Jia,Xinyao Zhou,Dandan Xie,Xiaojie Huang,Linyan Jia,Qian Zhou,Qingliang Zheng,Xiangyu Zhou,Kai Wang,Li-Ping Jin 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Preeclampsia is a pregnancy-specific disorder that is a major cause of maternal and fetal morbidity and mortality with a prevalence of 6–8% of pregnancies. Although impaired trophoblast invasion in early pregnancy is known to be closely associated with preeclampsia, the underlying mechanisms remain elusive. Here we revealed that lysyl oxidase (LOX) and LOX-like protein 2 (LOXL2) play a critical role in preeclampsia. Our results demonstrated that LOX and LOXL2 expression decreased in preeclamptic placentas. Moreover, knockdown of LOX or LOXL2 suppressed trophoblast cell migration and invasion. Mechanistically, collagen production was induced in LOX- or LOXL2-downregulated trophoblast cells through activation of the TGF-β1/Smad3 pathway. Notably, inhibition of the TGF-β1/Smad3 pathway could rescue the defects caused by LOX or LOXL2 knockdown, thereby underlining the significance of the TGF-β1/ Smad3 pathway downstream of LOX and LOXL2 in trophoblast cells. Additionally, induced collagen production and activated TGF-β1/Smad3 were observed in clinical samples from preeclamptic placentas. Collectively, our study suggests that the downregulation of LOX and LOXL2 leading to reduced trophoblast cell migration and invasion through activation of the TGF-β1/Smad3/collagen pathway is relevant to preeclampsia. Thus, we proposed that LOX, LOXL2, and the TGF-β1/Smad3/collagen pathway can serve as potential markers and targets for clinical diagnosis and therapy for preeclampsia.

Molecular cloning and expression analysis of the highly conserved eukaryotic translation initiation factor 5A (eIF-5A) from Antheraea pernyi

Dandan BIAN,Xiaoming ZHAO,Li CHEN,Jiwu TIAN,Qiuning LIU,Chunlin ZHOU,Boping Tang 한국곤충학회 2018 Entomological Research Vol.48 No.1

Eukaryotic initiation factor 5A (eIF‐5A) is a highly conserved protein found in all eukaryotic organisms that plays a key role in the regulation of many cellular processes including translation elongation, cell proliferation, programmed cell death, mRNA turnover and decay, and abiotic stress responses. In this study, the eIF‐5A gene from the Chinese oak silkworm Antheraea pernyi (Lepidoptera: Saturniidae) was characterized. The full‐length ApeIF‐5A cDNA of 1056 bp includes a 5′‐untranslated region (UTR) of 138 bp, a 3′‐UTR of 435 bp, and an open reading frame of 483 bp encoding a polypeptide of 160 amino acids. The deduced ApeIF‐5A protein shares 99 %, 82 %, and 72 % sequence identity with orthologs in Bombyx mori, Drosophila melanogaster and Homo sapiens, indicating high conservation during animal evolution. Real‐time quantitative reverse transcription PCR revealed expression in all four developmental stages and in all nine tissues tested, consistent with an important role in development. After challenge with lipopolysaccharide, the expression levels of ApeIF‐5A were markedly upregulated. Phylogenetic analysis of amino acid sequences revealed A. pernyi eIF‐5A was closely related to B. mori eIF‐5A, consistent with traditional classification and other molecular data. The results indicate the potential value of eIF‐5A in phylogenetic analysis.

KRAB family is involved in network shifts in response to osmotic stress in camels

Dandan Cao,Shenyuan Wang,Dong Zhang,Yanru Zhang,Junwei Cao,Yongbin Liu,Huanmin Zhou 한국통합생물학회 2022 Animal cells and systems Vol.26 No.6

A feature of the camel is its tolerance to osmotic stress. However, few studies of osmotic stress in vivo or comparative analyses between different tissues of the camel have been performed. Here, we report the roles of Krüppel-associated box domain containing zinc-finger repressor proteins (KRAB-ZFPs) in transcriptional networks under osmotic stress in camels by analyzing transcriptomes of four different tissues under various osmotic conditions. We found that 273 of 278 KRAB-ZFPs were expressed in our data set, being involved in all of the 65 identified networks and exhibiting their extensive functional diversity. We also found that 110 KRAB-ZFPs were hub genes involved in more than half of the networks. We demonstrated that the osmotic stress response is involved in network shifts and that KRAB-ZFPs mediate this process. Finally, we presented the diverse mechanisms of osmotic stress responses in different tissues. These results revealed the genetic architecture of systematic physiological response in vivo to osmotic stress in camels. Our work will lead to new directions for studying the mechanism of osmotic stress response in anti-arid mammals.

Robust and Fast Tracking via Joint Collaborative Representation

Fei Zhou,Guizong Zhang,Xinyue Fan,Dandan Yi 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.4

In this paper, we present a robust and fast tracking method based on joint collaborative representation. Traditional sparse coding based tracking methods code the candidates as a sparse linear combination of a series of object and trivial templates and perform time consuming L1 regularizations. In contrast to these methods, this paper adopts the L2-regularized least square models to reduce the computational complexity. The tracked object can be represented by the linear combination of a series of object templates, and also can be represented by candidate samples in the current frame. We propose a joint objective function to handle the tracking process. In addition, we introduce an effective update scheme to deal with the change of target appearance over time. Experiments on several challenging image sequences show that our proposed tracking method is robust and efficient.

Metallothionein MT1M Suppresses Carcinogenesis of Esophageal Carcinoma Cells through Inhibition of the Epithelial-Mesenchymal Transition and the SOD1/PI3K Axis

Li, Dandan,Peng, Weiyan,Wu, Bin,Liu, Huan,Zhang, Ruizhen,Zhou, Ruiqin,Yao, Lijun,Ye, Lin Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.4

Metallothionein (MT1M) belongs to a family of cysteinerich cytosolic protein and has been reported to be a tumor suppressor gene in multiple cancers. However, its role in esophageal carcinoma carcinogenesis remains unclear. In this study, MT1M expression was correlated with tumor type, stage, drinking and smoking history, as well as patient survival. We also studied the regulation and biological function of MT1M in esophageal squamous cell carcinoma (ESCC). We have found that MT1M is significantly downregulated in ESCC tissues compared with adjacent non-cancer tissues. Furthermore, restoration of expression by treatment with the demethylation agent A + T showed that MT1M downregulation might be closely related to hypermethylation in its promoter region. Over-expression of MT1M in ESCC cells significantly altered cell morphology, induced apoptosis, and reduced colony formation, cell viability, migration and epithelial-mesenchymal transition. Moreover, based on reactive oxygen species (ROS) levels, a superoxide dismutase 1 (SOD1) activity assay and protein analysis, we verified that the tumor-suppressive function of MT1M was at least partially caused by its upregulation of ROS levels, downregulation of SOD1 activity and phosphorylation of the SOD1 downstream pathway PI3K/AKT. In conclusion, our results demonstrated that MT1M was a novel tumor-suppressor in ESCC and may be disrupted by promoter CpG methylation during esophageal carcinogenesis.

Effective interfacially polymerized polyarylester solvent resistant nanofiltration membrane from liquefied walnut shell

Ayang Zhou,Ying Wang,Dandan Cheng,Mengying Li,Lei Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.6

Walnut shell, an agricultural waste, has not been efficiently utilized so far. In this paper, walnut shell wasliquefied first, followed by characterization of XPS and ATR-FTIR. It is shown that syringylphenol and guaiacylphenolare contained in the walnut shell. A novel organic solvent resistant nanofiltration (OSN) membrane was prepared byliquefied walnut shell (LWP) and trimethyl chloride (TMC) on the crosslinked polyetherimide substrate via interfacialpolymerization method. The results showed that LWP and TMC formed polyarylester toplayer. The NF-2LWP membranemaintained stable N,N-Dimethylformamide (DMF) permeance of 2.2 L m2 h1 MPa1 and rejection of 98% forcrystal violet (CV, 407.98 g mol1) in 36 hours continuous separation process. Furthermore, guaiacol (GA), a componentof LWP, was used to prepare an OSN membrane, Compared with NF-LWP membrane, NF-GA membrane showsgood performance in the separation of tetrahydrofuran (THF) with the rejection of 96% for rose bengal (RB, 1,017.64 gmol1) and the permeance of 76 L m2 h1 MPa1. This study not only provides a good way for the efficient recycling ofwalnut shell, but also provides a new component for the preparation of polyarylester OSN membrane.