KRAB family is involved in network shifts in response to osmotic stress in camels

Dandan Cao,Shenyuan Wang,Dong Zhang,Yanru Zhang,Junwei Cao,Yongbin Liu,Huanmin Zhou 한국통합생물학회 2022 Animal cells and systems Vol.26 No.6

A feature of the camel is its tolerance to osmotic stress. However, few studies of osmotic stress in vivo or comparative analyses between different tissues of the camel have been performed. Here, we report the roles of Krüppel-associated box domain containing zinc-finger repressor proteins (KRAB-ZFPs) in transcriptional networks under osmotic stress in camels by analyzing transcriptomes of four different tissues under various osmotic conditions. We found that 273 of 278 KRAB-ZFPs were expressed in our data set, being involved in all of the 65 identified networks and exhibiting their extensive functional diversity. We also found that 110 KRAB-ZFPs were hub genes involved in more than half of the networks. We demonstrated that the osmotic stress response is involved in network shifts and that KRAB-ZFPs mediate this process. Finally, we presented the diverse mechanisms of osmotic stress responses in different tissues. These results revealed the genetic architecture of systematic physiological response in vivo to osmotic stress in camels. Our work will lead to new directions for studying the mechanism of osmotic stress response in anti-arid mammals.

基于UDMISS理论的中国移动医疗APP质量和用户满意度的关系研究

曹峻玮 ( Junwei Cao ),张贵花 ( Guihua Zhang ),刘栋 ( Dong Liu ) 사단법인 아시아문화학술원 2021 인문사회 21 Vol.12 No.3

移动医疗APP近几年在中国快速发展, 特别是随着COVID-19的爆发, 越来越多的人开始通过移动医疗APP在线获取医疗健康服务。本研究以移动医疗APP质量、用户对移动医疗APP熟悉程度为研究视角, 分析了影响用户满意度的因素。基于更新的信息系统成功理论(UDMISS), 考察已有的相关研究, 建立了包括3个维度、4个变量的研究模型, 提出了和研究相关的假设, 并对移动医疗APP用户进行问卷调查, 使用PLS-SEM以及SmartPls3.0对问卷数据进行分析, 对研究模型进行评价, 并且对提出的假设进行了验证。通过本研究期待为移动医疗APP企业的发展和营销提供理论基础和实践指导。 Mobile medical APP have developed rapidly in China in recent years, especially with the explosion of COVID-19, more and more people have started to access medical and health services online through mobile medical APP. This study analyzes the factors affecting user satisfaction by taking the quality of mHealth APP and users’ familiarity with mHealth APP as the research perspective. Based on the updated theory of information system success (UDMISS), the existing related studies were examined, a research model including 3 dimensions and 4 variables was established, hypotheses related to the study were proposed, and a questionnaire survey was conducted on mHealth APP users, and the questionnaire data were analyzed using PLS-SEM and SmartPls3.0 to evaluate the research model, and the proposed The hypotheses were validated. This study is expected to provide theoretical basis and practical guidance for the development and marketing of mobile medical APP companies.

A Controllability Synthesis Problem for Dynamic Multi-Agent Systems with Linear High-Order Protocol

Ning Cai,Junwei Cao,M. Junaid Khan 제어·로봇·시스템학회 2014 International Journal of Control, Automation, and Vol.12 No.6

In this paper, a problem on controllability synthesis for dynamic multi-agent systems with high-order linear protocol is addressed. A particular scenario is considered, in which a given system should be adjusted to be controllable by the input information upon the created leaders, with optional communication links from the leaders to each follower vertex in the graph. It is shown that a single leader is always sufficient to satisfy the requirement for controllability of any given graph topology, so long as there are proper links between the leader and the followers.

Prevalence and risk factors for incidental prostate cancer in patients after transurethral resection of the prostate with negative results on prostate biopsy: A retrospective study

Zhenlang Guo,Junwei He,Jun Pan,Lijuan Huang,Jiadong Cao,Zunguang Bai,Shusheng Wang,Songtao Xiang,Chiming Gu,Zhaohui Wang 대한비뇨의학회 2022 Investigative and Clinical Urology Vol.63 No.2

Purpose: This study aimed to explore the prevalence and predictors of incidental prostate cancer (IPC) after transurethral resection of the prostate (TURP) with negative results on transperineal magnetic resonance imaging (MRI)/transrectal ultrasonography (TRUS) fusion prostate biopsy or TRUS-guided prostate biopsy. Materials and Methods: Data of 253 patients who underwent TURP with a preliminary diagnosis of benign prostatic hyperplasia (BPH) were evaluated. The prevalence of IPC was calculated. Univariate and multivariate logistic regression analyses were conducted to explore independent predictive factors of IPC. Results: A total of 253 patients were included. IPC was diagnosed in 12 patients (4.7%). The mean age of the patients and the mean prostate volume were 69.8±7.07 years and 89.3±49.29 mL, respectively. The prevalence of IPC was higher in the TRUS guided prostate biopsy group than in the transperineal MRI/TRUS fusion prostate biopsy group (11 of 203 [5.4%] vs. 1 of 50 [2.0%], p=0.47), but the difference was not statistically significant. Our results indicated that older age (≥70 y) (odds ratio [OR], 1.14; 95% confidence interval [CI], 1.02–1.27; p=0.025) and smaller prostate volume (OR, 0.97; 95% CI, 0.938–0.998; p=0.039) were associated with an increased incidence of IPC after TURP. Conclusions: Our findings indicate that the prevalence of IPC may be higher among patients who undergo transrectal prostate biopsy before TURP than among those who undergo transperineal MRI/TRUS fusion prostate biopsy. Older age and smaller prostate volume were independent predictors of increasing the risk for IPC after TURP.

在线教育平台游戏化元素对持续使用的影响机制研究: 基于S-O-R视角

张贵花 ( Guihua Zhang ),曹峻玮 ( Junwei Cao ),刘栋 ( Dong Liu ) 사단법인 아시아문화학술원 2021 인문사회 21 Vol.12 No.6

近几年在线教育快速发展, 特别是随着COVID-19的爆发, 越来越多的⼈开始通过在线教育平台进行学习。本研究从在线教育平台中的游戏元素切入, 分析影响⽤户持续使用的因素。基于刺激机体反应框架(S-O-R Framework), 在先行研究的基础上, 提出假设, 建⽴研究模型。对在线教育⽤户进⾏问卷调查后, 使⽤PLS-SEM以及SmartPls3.0进行假设验证。研究结果表明游戏化元素对在线教育用户的享受和持续使用有显著的积极影响, 同时发现用户的适应性显著的正向调节了游戏化元素和用户享受之间的关系。研究指出, 为了更好地激发用户兴趣, 在线教育企业应结合课程内容和用户特点, 精心设计游戏化体验。 Online education is developing rapidly in recent years. Especially with the outbreak of COVID-19, more and more people begin to learn through online education platform. This research starts with the elements of gamification in online education and analyzes the factors affecting users’ continuous use. Based on the Stimulus-Organism-Response Framework (SOR) and the previous researches, the hypothesis was put forward, and the research model was established. After the questionnaire survey of online education users, PLS-SEM and smartpls3.0 were used to verify the hypothesis. The research results show that gamification elements have a significant positive impact on the enjoyment and continuous use of online education users, and it is also found that users’ adaptability significantly positively regulates the relationship between gamification elements and users’ enjoyment. Research points out that in order to better stimulate user interest, online education companies should carefully design gamification experience in combination with course content and user characteristics.

Expression of Bacillus subtilis proBA Genes and Reduction of Feedback Inhibition of Proline Synthesis Increases Proline Production and Confers Osmotolerance in Transgenic Arabidopsis

Chen, Mingqing,Wei, Hongbo,Cao, JunWei,Liu, Ruijie,Wang, Youliang,Zheng, Congyi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

Proline accumulation has been shown to correlate with tolerance to drought and salt stresses in plants. We attempt to introduce the wild-type, mutant, and fusion proBA genes derived from Bacillus subtilis into Arabidopsis thaliana under the control of a strong promoter cauliflower mosaic virus 35S (CaMV35S). The transgenic plants produced higher level of free proline than control and the overproduction of proline resulted in the increased tolerance to osmotic stress in transgenic plants. Besides, the mutation in proBA genes, which were proved to lead $\alpha$-glutamyl kinase ($\alpha$-GK) reduces sensitivity to the end-product inhibition and the fusion of proB and proA also result in increasing proline production and confer osmotolerance in transgenic lines.

Effect of Scale-Dependent Viscosity and Transesterification on Filling Behavior of Polycarbonate/Poly(ethylene terephthalate) Blends in Micro-Injection Molding

Bozhi Cai,Jing Jiang,Yongjun Cao,Junwei Sun,Ning Zhang,Qian Li 한국고분자학회 2022 Macromolecular Research Vol.30 No.3

This study introduces a new scale-dependent viscosity model, in which transesterification reaction of polycarbonate/poly(ethylene terephthalate) (PC/PET) blends with various phase morphologies and micro-scale effects have been taken into account. It is found that a Power-law model can be used to quantitatively describe the relationship between the degree of transesterification and shear rate employed during compounding of PC and PET. A micro-scale viscosity model, which incorporates the micro-scale effects, has been developed by characterizing the flow using a double-barrel capillary rheometer with different microscale channels. For both neat PC and PC/PET blends, under the conditions of the constant shear rate and melt temperature, the shear viscosity dropped with decreasing capillary diameter because of the wall-slip effect. The proposed viscosity model based on the Cross equation can describe the variation of shear viscosity for PC/PET blends under both macro- and micro-scale conditions. Less than 7% average error is obtained between the model predictions and rheological experimental data. Filling simulation and micro-injection molding (μIM) short-shot experiments were conducted to validate the accuracy of the proposed viscosity model. For all the L9(34) design of experiment (DOE) molding trials, the average relative error under the micro-scale condition was 4.5±1.1%, which is much smaller than that of the average relative error under the macro-scale condition at 11.4±2.7%.