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생쥐 수정란의 보조부화술에 있어서 Non-Contact Type인 Diode Laser의 이용
김동훈,이명섭,강희규,한성원,김묘경,박원일,이훈택,정길생,이호준,Kim, D.H.,Lee, M.S.,Kang, H.G.,Han, S.W.,Kim, M.K.,Park, W.I.,Lee, H.T.,Chung, K.S.,Lee, H.J. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2
The present study was performed to investigate the efficacy and safety of laser assisted hatching (AH) on mouse embryos. Non-contact $1.48{\mu}m$ diode laser system used to create a precise hole on zona pellucida. 2-cell embryos were collected from the mice (ICR) that had the coitus vaginal plug confirmed at 48 hours after hCG injection. Collected 2-cell embryos were cultured in the HTF medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after 18-22 hours in culture. After assisted hatching, the embryos were further cultured in HTF medium containing 0.1% PVP (anti-hatching system) for 3 days. For evaluate efficiency of laser on mouse embryo hatching, the effect of AH methods (acidic tyrode, pronase and laser), the number of artificial holes (1, 2 and 3 hole) and the irradiation time of laser (2, 4, 6, 8 and 10 ms) were examined. Hatching rates of laser AH group (95.2%) was significantly higher than that of control group (50.8%), but there was no differences among the laser (95.2%), acidic tyrode (100%) and pronase (98.5%) groups. Hatching rates of the number of zona pellucida opening by laser, there were no differences among the 1 hole (87.5%),2 hole (92.1%) and 3 hole (85.9%) groups. Developmental and hatching rates of embryos according to laser irradiation time were similar in the treatment groups. Therefore, these results suggest that laser AH using non-contact $1.48{\mu}m$ diode laser is a simple and accurate and effective procedure for AH. Based on these results, laser AH could be use safely for human ART program.
생식보조시술시 단백질원으로서 인간난포액의 적합성 및 효율성에 관한 연구;II. 인간난포액이 생쥐수정란의 체외발달에 미치는 효과
김동훈,지희준,김지연,구정진,장상식,정길생,Kim, D.H.,Chi, H.J.,Kim, J.Y.,Koo, J.J.,Chang, S.S.,Chung, K.S. 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.1
This study was performed to investigate the effect of human follicular fluid (HFF) on development of mouse embryos, for evaluating the suitability of HFF as a substitutive material of human fetal cord serum in ART program. The various concentrations of HFF were added into the culture medium and the effects of HFF concentrations were examined to identify the optimal concentration of HFF for embryo development. The potency of HFF in improving embryo development was compared to that of other protein supplement. Collected HFFs were classified with the maturity of the containing oocytes; mature, immature, atretic, and then the effects of the classified HFFs on embryo development were examined. Also, HFF was separated into the low (<30,000 Da) and high (>30,000 Da) molecular weight fractions and the effects of the fractions on embryo development were investigated. The highest development rate was found in culture medium supplemented with 20% HFF, bnt this rate was reversely reduced at the concentrations of HFF higher than 20%. The development rates to the blastocyst, hatching blastocyst, attachment and outgrowth cultured in mature HFF was significantly higher than those in immature and atretic HFF, and mean cell number in blastocyst was higher in mature HFF than in immature and atretic HFF. The development rates of mouse embryos according to protein sources were significantly higher in HFF than in fetal cord serum (FCS), maternal serum (MS) and bovine serum albumin (BSA), and mean cell number in blastocyst cultured in HFF was higher than that in FCS, MS and BSA. The development rates of embryo and mean cell number in blastocyst cultured in high molecular weight fraction of HFF were higher than those in low molecular weight fraction, but the results of high molecular weight fraction were lower than those of whole HFF. Therefore, these results indicated that human mature follicular fluid was useful for improving the development of mouse embryos, which suggests a possibility that HFF also may be used efficiently for improving the culture condition in human ART program as a protein supplement.
TWO-Point Reactor Kinetics for Large D$_2$O Reflected Systems
노태완,오세기,김성년,김동훈,Noh, T.W.,Oh, S.K.,Kim, S.Y.,Kim, D.H. Korean Nuclear Society 1987 Nuclear Engineering and Technology Vol.19 No.3
다량의 중수반사체를 가진 조밀한 노심에서는 핵분열시 발생하는r선과 중수소와의 (r,n) 반응에 의해 지발 광중성자가 다량 생성되므로 이러한 계통을 기술하기 위하여 광중성자와 그 모핵종의 공간적 분리에 역점을 두어 2-점노 운동방정식을 정립하였다. 여러 반응도를 주입하여 출력 천이를 모사계산하므로써 노심과 반사체사이의 관련 효과를 조사하였다. 이 모델에 의한 모사계산 결과와 공간 종속 운동방정식에 의한 계산결과를 비교하였다. 반사체 영역에서의 광중성자 효과가 포함되므로써, 이를 포함하지 않은 모델에 비해 출력 천이현상을 감소시켰다. 실제로 출력을 측정하는 계측기는 이러한 공간적 분리영 향을 제거하기 위하여 노심 내부에 위치하여야 한다. Two-point kinetic equations for a compact-core-with-bulky-D$_2$O-reflector system were developed. A unique feature of the system is that certain fission gammas create retarded photoneutrons in the D$_2$O reflector by (r, n) reaction. Coupling effect between the core and the reflector was investigated by simulating power transients with various ramp reactivity insertions. Special attention was paid to the phenomenon associated with spatial separation of photoneutrons and their precursors. Simulations show that accuracy of the two-point model is comparable with that of space-dependent approach. Also it is found that the explicily expressed photoneutron terms in the reflector equation slow down the power transient compared to non-photoneutron expressions. Detectors for reactor power control purpose prefer to be deployed in the core zone to be able to accurately perdict transient power.