http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Virus-like Particles and Cellular Changes in Plants Infected with Sweetpotato Viruses
Sim, Jeong-Gu,Valverde, Rodrigo,Clark, Christopher,Chun, Se-Chul The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.1
Studies with the transmission electron microscope were used to detect and attempt to identify viruses infecting sweetpotato (Ipomoea batatas) and other Ipomoea species. Flexuous-rods, short curved-rods, and spherical virus-like particles were observed in cells of symptomatic plants. Also, various cytopathic changes such as crystals, vesicles, fibril structures, and cylindrical inclusions were observed. The present study showed that some of these cytopathic changes were associated with some viral groups, which might be helpful in diagnosis.
Validating lactate dehydrogenase (LDH) as a component of the PLASMIC predictive tool (PLASMIC-LDH)
Christopher Chin Keong Liam,Jim Yu-Hsiang Tiao,Yee Yee Yap,Yi Lin Lee,Jameela Sathar,Simon McRae,Amanda Davis,Jennifer Curnow,Robert Bird,Philip Choi,Pantep Angchaisuksiri,Sim Leng Tien,Joyce Ching Me 대한혈액학회 2023 Blood Research Vol.58 No.1
Background The PLASMIC score is a convenient tool for predicting ADAMTS13 activity of <10%. Lactate dehydrogenase (LDH) is widely used as a marker of haemolysis in thrombotic thrombocytopenic purpura (TTP) monitoring, and could be used as a replacement marker for lysis. We aimed to validate the PLASMIC score in a multi-centre Asia Pacific region, and to explore whether LDH could be used as a replacement marker for lysis. Methods Records of patients with thrombotic microangiopathy (TMA) were reviewed. Patients’ ADAMTS13 activity levels were obtained, along with clinical/laboratory findings relevant to the PLASMIC score. Both PLASMIC scores and PLASMIC-LDH scores, in which LDH replaced traditional lysis markers, were calculated. We generated a receiver operator characteristics (ROC) curve and compared the area under the curve values (AUC) to determine the predictive ability of each score. Results 46 patients fulfilled the inclusion criteria, of which 34 had ADAMTS13 activity levels of <10%. When the patients were divided into intermediate-to-high risk (scores 5‒7) and low risk (scores 0‒4), the PLASMIC score showed a sensitivity of 97.1% and specificity of 58.3%, with a positive predictive value (PPV) of 86.8% and negative predictive value (NPV) of 87.5%. The PLASMIC-LDH score had a sensitivity of 97.1% and specificity of 33.3%, with a PPV of 80.5% and NPV of 80.0%. Conclusion Our study validated the utility of the PLASMIC score, and demonstrated PLASMIC-LDH as a reasonable alternative in the absence of traditional lysis markers, to help identify high-risk patients for treatment via plasma exchange.
Virus-like Particles and Cellular Changes in Plants Infected with SweetpotatoViruses
Jeonggu Sim,Rodrigo Valverde,Christopher Clark,천세철 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.1
Studies with the transmission electron microscope were used to detect and attempt to identify viruses infecting sweetpotato (Ipomoea batatas) and other Ipomoea species. Flexuous-rods, short curved-rods, and spherical viruslike particles were observed in cells of symptomatic plants. Also, various cytopathic changes such as crystals, vesicles, fibril structures, and cylindrical inclusions were observed. The present study showed that some of these cytopathic changes were associated with some viral groups, which might be helpful in diagnosis.
High frequency <i>in situ</i> fatigue response of Ni-base superalloy René-N5 microcrystals
Lavenstein, Steven,Crawford, Bryan,Sim, Gi-Dong,Shade, Paul A.,Woodward, Christopher,El-Awady, Jaafar A. Elsevier 2018 Acta materialia Vol.144 No.-
<P><B>Abstract</B></P> <P>A novel <I>in situ</I> scanning electron microscope (SEM), high frequency fatigue testing methodology is developed using a combination of laser milling, focused ion beam fabrication and nanoindentation. This methodology is used to investigate crack initiation, propagation, fracture, fatigue life, and the mechanical response of microcantilever samples of a Ni-based superalloy (René-N5) under different cyclic strain amplitudes. The crack initiation and propagation in the microcantilever is monitored by observing changes in the beam's dynamic stiffness and continuous SEM imaging. The dynamic stiffness response of the micro-beams exhibits a transition from softening to hardening at a critical strain amplitude of 7 × <SUP> 10 − 3 </SUP> . Theoretical analysis indicates that this transition corresponds to the stress required to shear <SUP> γ ′ </SUP> precipitates. SEM imaging reveals the evolution of significant extrusions, intrusions, and slip traces during cyclic loading above this critical strain amplitude. Below this strain amplitude, very little surface roughening is observed. In addition, the measured dynamic stiffness is observed to exhibit two regimes of decrease after crack initiation. These two regimes correspond to short and large crack propagation. Finally, an overall increase in fatigue life is observed when comparing to bulk scale experiments on nickel-base superalloys. It is proposed that this is an inherent size effect in the small-volume, single crystal specimens tested.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Raehyun KIM,Peter ATTAYEK,Yuli WANG,Kathleen FURTADO,Rita TAMAYO,Christopher SIMS,Nancy ALLBRITTON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Healthy human bodies host hundreds of trillion microbes. More than 99% of these microbes colonize the gut. These gut microbes, also called gut microbiota, have huge impacts on the health and disease states of the hosts. An in vitro intestinal model system that enables co-culturing the gut microbes with human cells can be a valuable tool to elucidate their interaction. However, creating a model system that possesses a proper oxygen gradient to meet different oxygen demands of the gut microbes, primarily obligate anaerobic bacteria, and the human cells, has been challenging. Here, we show a simple in vitro human colon model system that creates the physiological oxygen gradient by utilizing the cellular oxygen consumption and modulating oxygen influx. The behaviors of the primary human colon epithelial cells in the oxygen gradient were compared to those cultured in the conventional aerobic condition. Multiple obligate anaerobic gut bacterial strains were successfully co-cultured in the model system. This novel and simple in vitro colon model system may be a valuable tool to study the interaction of gut bacteria and the host cells.
Short-term, Multi-center Prospective Clinical Study of Short Implants Measuring Less Than 7mm
Kim, Young-Kyun,Yi, Yang-Jin,Kim, Su-Gwan,Cho, Yong-Seok,Yang, Choon-Mo,Liang, Po-Chin,Chen, Yu-Yal,I, Lee-Long,Sim, Christopher,Tan, Winston,Ser, Go Wee,Yue, Deng,Yi, Man,Ping, Gong Korean Academy of Dental Science 2010 Journal of korean dental science Vol.3 No.1
Objective : This prospective study sought to verify the stability of three types of short implants measuring 7mm or less. Materials and methods : Implants measuring 7mm or less were placed in patients at multicenter dental clinics in Korea, China, Taiwan, and Singapore. Initial stability, intraoperative and postoperative complications, crestal bone loss, and survival rate of the implant were prospectively evaluated. Results : The primary stability of a 6-mm implant was lower than that of a 7-mm implant. The marginal bone loss of short implants measuring less than 7mm was minimal. Complications such as wound dehiscence, implant mobility, and peri-implant mucositis developed, and these were associated with initial implant failure. The short-term survival rate of 6-mm implant was 93.7%, and that of 7-mm implant, 96.6%. Conclusion : Short implant for the mandible with insufficient height for the residual ridge can be selectively used. Poor primary stability and wound dehiscence can cause osseointegration failure and alveolar bone loss.
Short-term, Multi-center Prospective Clinical Study of Short Implants Measuring Less Than 7mm
Young-Kyun Kim,Yang-Jin Yi,Su-Gwan Kim,Yong-Seok Cho,Choon-Mo Yang,Po-Chin Liang,Yu-Yal Chen,Lee-Long I,Christopher Sim,Winston Tan,Go Wee Ser,Deng Yue,Man Yi,Gong Ping 대한치의학회 2010 Journal of korean dental science Vol.3 No.1