Dislocation Density-Based Constitutive Model and Processing Map for T2 Copper During Isothermal and Time-Variant Deformation

Gang Chen,Yu Jin,Jing Wang,Cheng Zhang,Qiang Chen,Hongming Zhang,Xingjian Zhao,Zhiyong Li,Changhai Xie,Zhiming Du 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.9

Isothermal compression tests were carried out to study the hot deformation behavior of T2 copper under various conditions. The deformation parameters, such as temperature and strain rate, have strong influence on flow stress and microstructureevolution of the alloy. A unified dislocation density-based model considering dynamic recovery and recrystallization wasestablished. And material parameters of the developed model were optimized by genetic algorithm. Comparisons betweenthe experimental and model data demonstrates that the developed model can precisely describe the flow behavior at quitewide range of deformation conditions. Meanwhile, the designed iterative procedure allows the model to be applied in timevariantdeformation conditions. Processing map and microstructure examination were constructed to optimize the processingwindow of the studied alloy. According to the processing maps, flow instability mainly appeared at low temperatures of500–650 °C and strain rates higher than 0.1 s−1. The optimum deformation parameters of T2 copper was concluded as thetemperature range of 700–800 °C and the strain rate of 0.1–1 s−1.

Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy

Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.

In-pile tritium release behavior and the post-irradiation experiments of Li4SiO4 fabricated by melting process

Zhao Linjie,Yang Mao,Xiao Chengjian,Gong Yu,Ran Guangming,Chen Xiaojun,Li Jiamao,Yue Lei,Chen Chao,Hou Jingwei,Wang Heyi,Long Xinggui,Peng Shuming 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1

Understanding the tritium release and retention behavior of candidate tritium breeder materials is crucial for breeder blanket design. Recently, a melt spraying process was developed to prepare Li4SiO4 pebbles, which were subsequently subjected to the in-pile tritium production and extraction platform in China Mianyang Research Reactor (CMRR) to investigate their in-situ tritium release behavior and irradiation performance. The results demonstrate that HT is the main tritium release form, and adding hydrogen to the purge gas reduces tritium retention while increasing the HT percent in the purge gas. Post-irradiation experiments reveal that the irradiated pebbles darken in color and their grains swell, but the mechanical properties remain largely unchanged. It is concluded that the tritium residence time of Li4SiO4 made by melt spraying method at 467 ◦C is approximately 23.34 h. High-density Li4SiO4 pebbles exhibit tritium release at relatively low temperatures (<600 ◦C) that is mainly controlled by bulk diffusion. The diffusion coefficient at 525 ◦C and 550 ◦C is 1.19 × 10 11 cm2/s and 5.34 × 10 11 cm2/s, respectively, with corresponding tritium residence times of 21.3 hours and 4.7 hours.

A possible mechanism to the antidepressant-like effects of 20 (S)-protopanaxadiol based on its target protein 14-3-3 ζ

Chen, Lin,Li, Ruimei,Chen, Feiyan,Zhang, Hantao,Zhu, Zhu,Xu, Shuyi,Cheng, Yao,Zhao, Yunan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.5

Background: Ginsenosides and their metabolites have antidepressant-like effects, but the underlying mechanisms remain unclear. We previously identified 14-3-3 ζ as one of the target proteins of 20 (S)-protopanaxadiol (PPD), a fully deglycosylated ginsenoside metabolite. Methods: Corticosterone (CORT) was administered repeatedly to induce the depression model, and PPD was given concurrently. The tail suspension test (TST) and the forced swimming test (FST) were used for behavioral evaluation. All mice were sacrificed. Golgi-cox staining, GSK 3β activity assay, and Western blot analysis were performed. In vitro, the kinetic binding analysis with the Biolayer Interferometry (BLI) was used to determine the molecular interactions. Results: TST and FST both revealed that PPD reversed CORT-induced behavioral deficits. PPD also ameliorated the CORT-induced expression alterations of hippocampal Ser9 phosphorylated glycogen synthase kinase 3β (p-Ser9 GSK 3β), Ser133 phosphorylated cAMP response element-binding protein (p-Ser133 CREB), and brain-derived neurotrophic factor (BDNF). Moreover, PPD attenuated the CORT-induced increase in GSK 3β activity and decrease in dendritic spine density in the hippocampus. In vitro, 14-3-3 ζ protein specifically bound to p-Ser9 GSK 3β polypeptide. PPD promoted the binding and subsequently decreased GSK 3β activity. Conclusion: These findings demonstrated the antidepressant-like effects of PPD on the CORT-induced mouse depression model and indicated a possible target-based mechanism. The combination of PPD with the 14-3-3 ζ protein may promote the binding of 14-3-3 ζ to p-GSK 3β (Ser9) and enhance the inhibition of Ser9 phosphorylation on GSK 3β kinase activity, thereby activating the plasticity-related CREBeBDNF signaling pathway.

Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168

Chen Zhao,Xiang-Ying Zhao,Jian-Jun Liu,Jun-Jiao Zhang,Jia-Xiang Zhang,Li-He Zhang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.5

D-ribose, a five-carbon sugar with diverse applications, is mainly produced by transketolase (tkt)-deficient Bacillus subtilis (B. Subtilis). We constructed B.subtilis SFR-3A by replacing the corresponding sites of B. subtilis 168 with the mutation site of tkt in the “wild” D-ribose high-producing strain B. subtilis SFR-4, resulting in 5.29 g/L of Dribose. In the meantime, B.subtilis SFR-159 was constructed by completely removing the tkt gene of B. subtilis 168 with a higher production of 6.21 g/L. Through medium optimization, batch fermentation of SFR-3A and SFR-159 gave the best result of 27.56 g/L and 29.89 g/L, which corresponds to productivity of 0.51 g/L/h and 0.41 g/L/h, respectively. In this work, SFR-3A showed more latent capacity over SFR-159 as to productivity and had greater potential to serve as a platform for D-ribose production.

γ-Fe₂O₃ nanoparticles aligned in porous carbon nanofibers towards long life-span lithium ion batteries

Chen, Yujie,Zhao, Xiaohui,Liu, Ying,Razzaq, Amir Abdul,Haridas, Anupriya K.,Cho, Kwon-Koo,Peng, Yang,Deng, Zhao,Ahn, Jou-Hyeon Elsevier 2018 ELECTROCHIMICA ACTA Vol.289 No.-

<P><B>Abstract</B></P> <P>A freestanding γ-Fe<SUB>2</SUB>O<SUB>3</SUB>/carbon nanofiber composite as the anode of lithium ion batteries has been prepared with γ-Fe<SUB>2</SUB>O<SUB>3</SUB> nanoparticles aligned in the tubular channels of the nanofibrous carbon matrix. Innovatively, a large amount of voids are generated for confining a very high active mass content by facilely verifying the ratio of carbon source and the sacrificing pore-forming agent, poly(methyl methacrylate). The best electrochemical performance at an active mass ratio of 70% is obtained with a high discharge capacity of 1088 mAh g<SUP>−1</SUP> retained after 300 cycles at 0.2 C. Good rate capability and long life-span performance are further achieved at 0.5 and 2 C for 600 and 1000 cycles, respectively. <I>In situ</I> X-ray patterns further reveal better exploitation of the active materials for this γ-Fe<SUB>2</SUB>O<SUB>3</SUB>/carbon nanofiber composite electrode. The enhanced electrochemical performance of lithium ion batteries can be ascribed to the well-dispersed γ-Fe<SUB>2</SUB>O<SUB>3</SUB> nanoparticles within the porous carbon matrix, as well as the cross-linked fiber morphology. Consequently, this study provides a superior electrode architecture for constructing flexible anodes of high-performance lithium ion batteries.</P> <P><B>Graphical abstract</B></P> <P>The freestanding γ-Fe<SUB>2</SUB>O<SUB>3</SUB>/carbon nanofiber composite with hierarchical porous structure promotes the development of long life-span lithium ion batteries.</P> <P>[DISPLAY OMISSION]</P>

R&D Performance in High-Tech Firms in China

Chen, Zhao,Lee, Sang-Ho,Xu, Wei MIT Press 2017 ASIAN ECONOMIC PAPERS Vol.16 No.3

Removal of the Glycosylation of Prion Protein Provokes Apoptosis in SF126

Chen, Lan,Yang, Yang,Han, Jun,Zhang, Bao-Yun,Zhao, Lin,Nie, Kai,Wang, Xiao-Fan,Li, Feng,Gao, Chen,Dong, Xiao-Ping,Xu, Cai-Min Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Although the function of cellular prion protein (PrP$^C$) and the pathogenesis of prion diseases have been widely described, the mechanisms are not fully clarified. In this study, increases of the portion of non-glycosylated prion protein deposited in the hamster brains infected with scrapie strain 263K were described. To elucidate the pathological role of glycosylation profile of PrP, wild type human PrP (HuPrP) and two genetic engineering generated non-glycosylated PrP mutants (N181Q/N197Q and T183A/T199A) were transiently expressed in human astrocytoma cell line SF126. The results revealed that expressions of non-glycosylated PrP induced significantly more apoptosis cells than that of wild type PrP. It illustrated that Bcl-2 proteins might be involved in the apoptosis pathway of non-glycosylated PrPs. Our data highlights that removal of glycosylation of prion protein provokes cells apoptosis.

Relationships of Low Serum Levels of Interleukin-10 With Poststroke Anxiety and Cognitive Impairment in Patients With Clinical Acute Stroke

Zhao-jian Ying,Yuan-Yuan Huang,Meng-Meng Shao,Chu-Huai Chi,Ming-Xia Jiang,Yi-Hui Chen,Yu-Chen,Miao-Xuan Sun,Yan-Yan Zhu,Xianmei Li 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.3

Background and Purpose The relationships among interleukin (IL)-10 levels, anxiety, and cognitive status after stroke remain controversial. We aimed to determine the associations of serum IL-10 levels with poststroke anxiety (PSA) and poststroke cognitive impairment (PSCI). Methods We recruited 350 patients with stroke, of whom only 151 completed a 1-month follow- up assessment. The Mini Mental State Examination (MMSE) and Hamilton Anxiety Scale (HAMA) were used to assess the cognitive status and anxiety, respectively. Serum IL-10 levels were measured within 24 hours of admission. Results IL-10 levels were significantly lower in the PSA group than in the non-PSA group, and they were negatively associated with HAMA scores (r=-0.371, p<0.001). After adjusting for all potential confounders, IL-10 levels remained an independent predictor of PSA (odds ratio=0.471, 95% confidence interval=0.237–0.936, p=0.032). IL-10 levels were strongly correlated with behavior during interviews, psychic anxiety, and somatic anxiety. Patients without PSCI had higher IL-10 levels were higher in non-PSCI patients than in PSCI patients, and they were positively associated with MMSE scores in the bivariate correlation analysis (r=0.169, p=0.038), and also with memory capacity, naming ability, and copying capacity. However, IL-10 did not predict PSCI in the univariable or multivariable logistic regression. Conclusions Low IL-10 levels were associated with increased risks of PSA and PSCI at a 1-month follow-up after stroke. Serum IL-10 levels may therefore be helpful in predicting PSA.

High Expression of Bcl-2 Protein Predicts Favorable Outcome in Non-small Cell Lung Cancer: Evidence from a Systematic Review and Meta-analysis

Zhao, Xian-Da,He, Yu-Yu,Gao, Jun,Zhao, Chen,Zhang, Ling-Lin,Tian, Jing-Yuan,Chen, Hong-Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: The prognostic value of Bcl-2 protein expression in non-small cell lung cancer (NSCLC) is under debate. We therefore systematically reviewed the evidence for Bcl-2 protein effects on NSCLC survival to elucidate this issue. Materials and Methods: An electronic search in Pubmed and Embase complemented by manual searches in article references were conducted to identify eligible studies to evaluate the association between Bcl-2 protein expression and overall survival (OS) as well as disease free survival (DFS) of NSCLC patients. Combined hazard ratios (HRs) with corresponding 95% confidence intervals (95%CIs) were pooled using the random-effects model. Results: A total of 50 trials (including 52 cohorts) encompassing 7,765 patients were pooled in the meta-analysis regarding Bcl-2 expression and OS of NSCLC patients. High expression of Bcl-2 protein had a favorable impact (HR=0.76, 95%CI=0.67-0.86). In the group of Bcl-2 expression and DFS, 11 studies including 2,634 patients were included. The synthesized result indicated high expression of Bcl-2 protein might predict good DFS (HR=0.85, 95%CI=0.75-0.95). Conclusions: Our present meta-analysis demonstrated favorable prognostic values of Bcl-2 expression in patients with NSCLC. Further prospective trails are welcomed to validate the utility of assessing Bcl-2 in NSCLC patient management.