Apoptosis induced in vivo by new type gosling viral enteritis virus

Shun Chen,Anchun Cheng,Mingshu Wang,Dekang Zhu,Renyong Jia,Qihui Luo,Hengmin Cui,Yi Zhou,Yin Wang,Zhiwen Xu,Zhengli Chen,Xiaoyue Chen,Xiaoyu Wang 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.4

In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease.

The Application of ANP Models in the Web-Based Course Development Quality Evaluation of Landscape Design Course

Xiaoyu Chen,Lifang Qiao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.9

Web-based course is the important method in the landscape design teaching. In this paper, the ANP (Analytic Network Process) model has been adopted to evaluate the quality of the development of on-line courses, so as to provide the basis for preferred on-line courses. Conclude 10 evaluation indicators from 3 aspects of teaching, availability, and technology, and establish ANP model consisting of control layer, network layer, and alternatives. Four web-based courses of landscape design were chosen as the evaluation objects and the SD (Super Decisions) software was used to conduct the calculation. The result shows that: ANP is a practical science decision analysis method, comprehensively considering the mutually influential and feedback relationships of indicators, and is suitable for the evaluation and decision of the complex problems. The development quality evaluation of ANP which is applied in landscape design web-based courses is feasible, and the result of research can provide decision basis to web-based course development.

Nicotinamide Mononucleotide Adenylyl Transferase 2 Inhibition Aggravates Neurological Damage after Traumatic Brain Injury in a Rat Model

Xiaoyu Gu,Haibo Ni,XuGang Kan,Chen Chen,Zhiping Zhou,Zheng Ding,Di Li,Bofei Liu 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.4

Objective : Nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) is a crucial factor for the survival of neuron. The role of NMNAT2 in damage following traumatic brain injury (TBI) remains unknown. This study was designed to investigate the role of NMNAT2 in TBI-induced neuronal degeneration and neurological deficits in rats. Methods : The TBI model was established in Sprague-Dawley rats by a weight-dropping method. Real-time polymerase chain reaction, western blot, immunofluorescence, Fluoro-Jade C staining, and neurological score analyses were carried out. Results : NMNAT2 mRNA and protein levels were increased in the injured-side cortex at 6 hours and peaked 12 hours after TBI. Knocking down NMNAT2 with an injection of small interfering RNA in lateral ventricle significantly exacerbated neuronal degeneration and neurological deficits after TBI, which were accompanied by increased expression of BCL-2-associated X protein (Bax). Conclusion : NMNAT2 expression is increased and NMNAT2 exhibits neuroprotective activity in the early stages after TBI, and Bax signaling pathway may be involved in the process. Thus, NMNAT2 is likely to be an important target to prevent secondary damage following TBI.

Adapative Load Anti-islanding Method of Large Distributed Photovoltaic Power Grid

Xiaoyu Chen,Jianyong Zheng,Jun Mei 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.8

A large number of distributed photovoltaic power grid, islanding problems become more prominent. This paper first analyzes some existing anti-islanding scheme, and analyzes its advantages and disadvantages. Then, this paper proposes adaptive anti-islanding scheme by using the load, i.e. load automatic switching, changing the characteristics of the whole load, make the passive anti-islanding without dead-zone. Then using PSCAD simulation of the program, to verify the usefulness of the proposed scheme. To minimize the distributed photovoltaic power grid investment, saving the scheme relies on a new generation of distributed photovoltaic (pv) grid interface device. Then the paper introduces the overall structure and function of the device configuration, the device not only can solve the problem of area of backup protection, also save on investment, convenient for on-site equipment installation and maintenance, for the widespread promotion of distributed photovoltaic power grid to lay a solid foundation.

Piezoelectric properties of [Li0.03(K0.48Na0.52)0.97](Nb0.97Sb0.03)O3-(Ba0.85Ca0.15)(Ti0.90Zr0.10)O3 lead-free piezoelectric ceramics

Xiaoyu Chen,Jiagang Wu,Xiaojing Cheng,Bo Wu,Wenjuan Wu,Dingquan Xiao,Jianguo Zhu 한국물리학회 2012 Current Applied Physics Vol.12 No.3

[Li0.03(K0.48Na0.52)0.97](Nb0.97Sb0.03)O3-(Ba0.85Ca0.15)(Ti0.90Zr0.10)O3 [(1-x)LKNNS-xBCTZ] lead-free piezoelectric ceramics were prepared by the conventional solid state method, and effects of BCTZ content on the piezoelectric properties of LKNNS ceramics were mainly investigated. A stable solid solution has been formed between LKNNS and BCTZ, and a morphotropic phase boundary of (1-x)LKNNS-xBCTZ ceramics is identified in the range of 0 < x ≤ 0.02. The Curie temperature of (1-x)LKNNS-xBCTZ ceramics decreases with increasing BCTZ content. A higher εr value and a lower tan d value are demonstrated for the (1-x)LKNNS-xBCTZ ceramic with x = 0.02. The (1-x)LKNNS-xBCTZ ceramic with x = 0.02 has an enhanced electrical behavior of d33w237 pC/N, kpw 48.6%, 3 rw1451, tan d w0.037, and Tcw335 ℃. As a result, (1-x)LKNNS-xBCTZ ceramics are promising candidate materials for the field of lead-free piezoelectric materials.

Applicable Prospects of Eye Tracking Technology in the Research of Landscape Visual Perception

Xiaoyu Chen,Lifang Qiao 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.10

This paper mainly adopts the literature research method, which analyzes the application progress and development overview of the eye tracking technology, as well as the technical features of the eye tracking devices. Combined with the research needs in landscape visual perception, eye tracking technology have application values in the following aspects: landscape attractiveness analysis, safety design of landscape environment, usability design of landscape facilities, landscape perception in motion, quality improvement of landscape information interface, cognitive analysis of physiological vulnerable groups and quality improvement of landscape education. Eye- tracking technology should be used reasonably in the study so as to exploit its advantages to the full and correct its deficiencies. The development of hardware technology and software technology of eye-tracking offers a new perspective and study platform for the study on landscape visual perception, which contributes to improving the scientificity of the study on landscape visual perception.

Performance of Mg-Ti modified iron-based catalyst in NH3-SCR of NO at the presence of arsenic: Influence of oxygen and temperature

Xiaoyu Li,Juan Chen,Shouyan Chen,Chunmei Lu,Guangqian Luo,Hong Yao 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-

Impact of arsenic on the performance of Mg-Ti modified iron-based catalyst during selective catalyticreduction of NOx with ammonia was studied. The results indicated that Mg and Ti modification not onlyimproved the catalytic activity at 100–350 C, but also exhibited remarkable anti-arsenic ability. Mg wasdominant in the excellent anti-arsenic performance, and this role was firstly recognized. The anti-arsenicmechanism of Mg-Ti modified iron-based catalyst was ascribed to two aspects. One side, Mg exhibitedwell reactivity with arsenic which competed with Fe for the adsorption of arsenic and protected activeiron sites from arsenic occupation. The other side, presence of Mg promoted the formation of MgFe2O4in view of the strong interactions between Mg and Fe, suppressing the interactions between arsenicand Fe. Instead, gaseous arsenic turned to bond with Mg-O sites. Consequently, surface acid sites andfunction Fe-OH/ Fe = O bonds were preserved for de-NOx. Moreover, increase of oxygen content and temperatureweakened the deactivation of Mg-Ti modified iron-based catalyst by arsenic. It was due to theoxidation of As2O3 with oxygen content increase and the strengthened reactivity of non-active iron siteswith arsenic and Mg with arsenic as temperature elevation, inhibiting the interactions between activeiron sites with arsenic.

基于建构主义学习理论的幼儿园劳动教育课程建构—以小班幼儿自我服务为例

陈子琳(Zilin Chen),陈晓雨(Xiaoyu Chen),李树燕(Shuyan Li) YIXIN 출판사 2024 教育教学研究论丛 Vol.2 No.7

Early childhood is an important stage for educational enlightenment, thought leadership and behaviour development, and with the gradual attention paid by the state to labour education, labor education for young children has become an important educational content. In the context of physical, aesthetic and labour curriculum construction and kindergarten high-quality development, the study found that the kindergarten labour curriculum has problems such as ambiguous objectives, thin content, sporadic practice and little evaluation. According to the four basic views of constructivist learning theory, taking self-service labour education in small classes as an example, we construct a labour education curriculum system, emphasizing that it covers three dimensions and points to multi-development in terms of curriculum objectives, closely follows the life and integrates the fields in terms of curriculum content, enriches the forms and makes good use of situations and games in terms of curriculum implementation, and diversifies the main subjects and strengthens the process in terms of curriculum evaluation. 幼儿期是教育启蒙、思想引领和行为发展的重要阶段,随着国家对劳动教育的逐步重视,幼儿劳动教育也成了重要的教育内容。在体美劳课程建设和幼儿园高质量发展的背景下,研究发现幼儿园劳动课程存在目标模糊、内容单薄、实践零星、评价鲜有等问题。根据建构主义学习理论的四个基本观点,以小班自我服务劳动教育为例,建构劳动教育课程体系,强调在课程目标上涵盖三维度、指向多发展,在课程内容上紧扣生活、融合领域,在课程实施上丰富形式、善用情境和游戏,在课程评价上多元主体、强化过程。

Quantum Public-key Cryptosystem without Quantum Channels between Any Two Users using Non-orthogonal States

Xiaoyu Li,Yuwen Chen 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9

A quantum public-key cryptosystem without quantum channels between any two users using non-orthogonal states is provided in this paper. Every user keeps a set of quantum particles in non-orthogonal states in a key management center (KMC) as the public key while he or she keeps the states of them as the private key. By the help of KMC users can accomplish secret communication and message authentication. The laws of quantum physics guarantee the unconditional security of this cryptosystem. No entangled states or complex quantum operations are needed. On the other hand there are no quantum channels needed to connecting any two users. So the public-key cryptosystem is easier to carry out and more robust in practice.

Prediction of Cellulose Crystallinity in Liquid Phase Using CBM-GFP Probe

Xiaoyu Guo,Fan Yang,Huixue Liu,Yingmin Hou,Yafang Wang,Jie Sun,Xiaoyi Chen,Yanan Liu,Xianzhen Li 한국고분자학회 2019 Macromolecular Research Vol.27 No.4

Carbohydrate-binding modules (CBMs) have been developed to investigate the presence of crystalline and amorphous regions of cellulose. However, systematic and quantitative assessment of cellulose crystallinity using such non-hydrolytic fusion proteins in liquid phase has not been reported. In this work, cellulose directed CBM probes containing a green fluorescent protein (GFP) were constructed and named CG17, CG28, and CG2a. The probe binding condition was determined as incubating 30 μg/mL probes in 10 mM phosphate buffer at 30oC for 60 min. Under the optimized condition, the linear correlations between CBM probe binding capability and X-ray diffraction (XRD) crystallinity were well established. Using linear regression equations, the crystallinity of several cellulosic materials was well calculated. Amorphous component and cellulosic surface area probably had a less effect on binding capability of CG2a than that of CG17 and CG28. Therefore, crystalline-region specific probe CG2a should be an efficient tool for interpreting the crystallinity of cellulosic materials.