ETV4 facilitates proliferation, migration, and invasion of liver cancer by mediating TGF-β signal transduction through activation of B3GNT3

Zhou Zhongcheng,Wu Bin,Chen Jing,Shen Yiyu,Wang Jing,Chen Xujian,Fei Faming,Li Liang 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background Metastasis of liver cancer (LC) is the main cause of its high mortality. ETV4 is a critical regulatory factor in promoting LC progression, but the mechanism that ETV4 impacts LC proliferation, migration, and invasion is poorly understood. Objective Investigation of the molecular mechanism of LC metastasis is conducive to developing effective drugs that prevent LC metastasis. Methods Expression of ETV4 and its target gene B3GNT3 in LC tissue was analyzed by bioinformatics, and the result was further verified in LC cells by qRT-PCR. In vitro cellular assays evaluated the impact of ETV4 on the proliferation, migration, and invasion of LC cells. Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter gene assay were conducted to analyze the interaction between B3GNT3 and ETV4. SB525334 suppressor was used to treat and access the activation of ETV4 on the TGF-β pathway. Results We discovered that ETV4 and B3GNT3 were evidently up-regulated in LC, and high expression of ETV4 was coupled to the increase of proliferation, migration, and invasion of LC cells and epithelial-mesenchymal transition ability. Besides, ETV4 could bind to the B3GNT3 promoter and activate its transcription. Knockdown of B3GNT3 could prominently suppress the effect of up-regulated ETV4 on LC cells. Meanwhile, ETV4 could activate the TGF-β signaling pathway via B3GNT3, while SB525334 treatment notably repressed the functions of ETV4. Conclusion ETV4 emerges as a driven oncogene in LC, and the ETV4/B3GNT3-TGF-β pathway promotes proliferation, migration, invasion, and epithelial-mesenchymal transition progress of LC. Inhibition of the pathway may provide an underlying method for the prevention and treatment of LC metastasis.

Acetate-assisted Synthesis of Chromium(III) Terephthalate and Its Gas Adsorption Properties

Zhou, Jing-Jing,Liu, Kai-Yu,Kong, Chun-Long,Chen, Liang Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

We report a facile synthetic approach of high-quality chromium(III) terephthalate [MIL-101(Cr)] by acetate-assisted method in the absence of toxic HF. Results indicate that the morphology and surface area of the MIL-101(Cr) can be tuned by modifying the molar ratio of acetate/$Cr(NO_3)_3$. The Brunauer-Emmett-Teller (BET) surface area of MIL-101(Cr) synthesized at the optimized condition can exceed 3300 $m^2/g$. It is confirmed that acetate could promote the dissolution of di-carboxylic linker and accelerate the nucleation ratio. So the pure and small size of MIL-101(Cr) with clean pores can be obtained. $CO_2$, $CH_4$ and $N_2$ adsorption isotherms of the samples are studied at 298 K and 313 K. Compared with the traditional method, MIL-101(Cr) synthesized by acetate-assisted method possess enhanced $CO_2$ selective adsorption capacity. At 1.0 bar 298 K, it exhibits 47% enhanced $CO_2$ adsorption capacity. This may be attributed to the high surface area together with clean pores of MIL-101(Cr).

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.

Comprehensive Analysis of Gut Microbiota Alteration in the Patients and Animal Models with Polycystic Ovary Syndrome

Zhou Jing,Qiu Xuemei,Chen Xuejing,Ma Sihan,Chen Zhaoyang,Wang Ruzhe,Tian Ying,Jiang Yufan,Fan Li,Wang Jingjie 한국미생물학회 2023 The journal of microbiology Vol.61 No.9

Polycystic ovary syndrome (PCOS) is a common disease of endocrine–metabolic disorder, and its etiology remains largely unknown. The gut microbiota is possibly involved in PCOS, while the association remains unclear. The comprehensive analysis combining gut microbiota with PCOS typical symptoms was performed to analyze the role of gut microbiota in PCOS in this study. The clinical patients and letrozole-induced animal models were determined on PCOS indexes and gut microbiota, and fecal microbiota transplantation (FMT) was conducted. Results indicated that the animal models displayed typical PCOS symptoms, including disordered estrous cycles, elevated testosterone levels, and ovarian morphological change; meanwhile, the symptoms were improved after FMT. Furthermore, the microbial diversity exhibited disordered, and the abundance of the genus Ruminococcus and Lactobacillus showed a consistent trend in PCOS rats and patients. The microbiota diversity and several key genera were restored subjected to FMT, and correlation analysis also supported relevant conclusions. Moreover, LEfSe analysis showed that Gemmiger, Flexispira, and Eubacterium were overrepresented in PCOS groups. Overall, the results indicate the involvement of gut microbiota in PCOS and its possible alleviation of endocrinal and reproductive dysfunctions through several special bacteria taxa, which can function as the biomarker or potential target for diagnosis and treatment. These results can provide the new insights for treatment and prevention strategies of PCOS.

Acetate-assisted Synthesis of Chromium(III) Terephthalate and Its Gas Adsorption Properties

Jing-jing Zhou,Kai-yu Liu,Chun-long Kong,Liang Chen 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.6

We report a facile synthetic approach of high-quality chromium(III) terephthalate [MIL-101(Cr)] by acetateassisted method in the absence of toxic HF. Results indicate that the morphology and surface area of the MIL- 101(Cr) can be tuned by modifying the molar ratio of acetate/Cr(NO3)3. The Brunauer-Emmett-Teller (BET) surface area of MIL-101(Cr) synthesized at the optimized condition can exceed 3300 m2/g. It is confirmed that acetate could promote the dissolution of di-carboxylic linker and accelerate the nucleation ratio. So the pure and small size of MIL-101(Cr) with clean pores can be obtained. CO2, CH4 and N2 adsorption isotherms of the samples are studied at 298 K and 313 K. Compared with the traditional method, MIL-101(Cr) synthesized by acetate-assisted method possess enhanced CO2 selective adsorption capacity. At 1.0 bar 298 K, it exhibits 47% enhanced CO2 adsorption capacity. This may be attributed to the high surface area together with clean pores of MIL-101(Cr).

IQGAP1 is overexpressed in hepatocellular carcinoma and promotes cell proliferation by Akt activation

Chen, Feng,Zhu, Hai-Hong,Zhou, Lin-Fu,Wu, Shan-Shan,Wang, Jing,Chen, Zhi Korean Society for Biochemistry and Molecular Bion 2010 Experimental and molecular medicine Vol.42 No.7

The scaffold protein IQGAP1 shows elevated levels in several cancer types, but its expression in hepatocellular carcinoma is unknown. We found that 58% of human hepatocellular carcinoma tissue samples had increased IQGAP1 expression compared to adjacent normal tissue. Overexpressing IQGAP1 raised the in vivo tumorigenicity of hepatocellular carcinoma cells, and forced overexpression of IQGAP1 in vitro stimulated cell proliferation. Cell growth was reduced by knockdown or mutation of IQGAP1, or by treatment of cells with a phosphotidylinositol 3-kinase inhibitor. To determine the mechanism by which IQGAP1 overexpression affected hepatocellular carcinoma cells, we confirmed its interaction in these cells with mammalian target of rapamycin (mTOR), a serine/threonine kinase that integrates signals about nutrient and energy status with downstream effectors that influence cell division. In addition, we discovered a new interaction involving IQGAP1, mTOR and Akt, which is a downstream target of mTOR. Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. We hypothesize that IQGAP1 is a scaffold that facilitates mTOR and Akt interaction.

A NEW TWO-DIMENSIONAL ANALYTICAL SOLUTION OF GROUNDWATER FLUCTUATIONS IN COASTAL AQUIFER

CHEN JING,ZHOU ZHIFANG 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Innovation Network Structure of Industrial Cluster of New Energy Vehicles in the Northeast China

Chen Wei,Jing Rui,Zhou Wen,LIN Chaoran 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.6

The new energy vehicles industry is the strategic development direction of China’s vehicles industry when there is an important need for this industry to realize the goal of energy conservation and environment protection, and of industry transformation and upgrading. It is an important approach that cooperatively innovating in the innovation network of industrial cluster to improve the industry’s innovation level and core competitiveness. Based on the empirical investigation of the innovation network of industrial cluster of new energy vehicles in the northeast China, this paper uses complex network theory to study the network structure from the perspectives of whole network, community and ego network of Hub. Moreover, this paper further discusses the matching problem of Hubs’ influence in the network. In addition, the relative suggestions about network governance on the innovation network of industrial cluster of new energy vehicles in the northeast China are proposed.

Influence of Screw Rotors Tip Angle on Mixing Performance for One Novel Twin-screw Kneader

Jing Wei,Dabing Chen,Dongming Zhou,Aiqiang Zhang,Yuliang Yang 한국고분자학회 2015 폴리머 Vol.39 No.3

Twin-screw kneader is an efficient polymer processing equipment. In this paper, the mixing performance of one novel intermeshing counter-rotating twin-screw kneader with different tip angles of the male rotor is simulated using the mesh superimposition technique (MST). Statistical analysis is carried out for the flow field using particle tracking technique, and distributive mixing performance is evaluated using the residence time distribution and segregation scale, while the dispersive mixing performance is estimated using the parameters such as shear rate, stretching rate and mixing index. The results show that the best distributive mixing performance is achieved when the tip angle is 0˚, while the optimal dispersive mixing performance is obtained when the tip angle is 20˚. The results in this paper provide a data basis for the selection of parameters and optimization of the performance for the screw rotors.

The inhibitory effect of sodium baicalin on oseltamivir-resistant influenza A virus via reduction of neuraminidase activity

Jing Jin,Yuanjin Chen,Dechuan Wang,Lingman Ma,Min Guo,Changlin Zhou,Jie Dou 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.6

Baicalin was identified as a neuraminidase (NA)inhibitor displaying anti-influenza A virus (IAV) activity. However, its poor solubility in saline has limited its use inthe clinic. We generated sodium baicalin and showed that itexhibited greatly increased solubility in saline. Its efficacyagainst oseltamivir-resistant mutant A/FM/1/47-H275Y(H1N1-H275Y) was evaluated in vitro and in vivo. Resultsshowed that 10 lM of sodium baicalin inhibited A/FM/1/47 (H1N1), A/Beijing/32/92 (H3N2) and H1N1-H275Y inMDCK cells in a dose-dependent manner, with inhibitoryrates of 83.9, 75.9 and 47.7%, respectively. Intravenousadministration of sodium baicalin at 100 mg/kg/d enabledthe survival of 20% of H1N1-H275Y-infected mice. Thetreatment alleviated body weight loss and lung injury. Moreover, sodium baicalin exerted a clear inhibitory effecton NAs. The IC50 values of sodium baicalin against H1N1-H275Y and cells-expressing A/Anhui/1/2013-R294K(H7N9-R294K) NA protein (N9-R294K) were 214.4 lMand 216.3 lM. Direct interactions between sodium baicalinand NA were observed, and we simulated the interactionsof sodium baicalin with N9-R294K and N9 near the activesites of OC-N9-R294K and OC-N9. The residues responsiblefor the sodium baicalin-N9-R294K and sodiumbaicalin-N9 interactions were the same, confirming thatsodium baicalin exerts effects on wild-type and oseltamivir-resistant viral strains.