Effect of Green Tea Extract Formulated with Crude Polysaccharides and Flavonols Derived from Green Tea as Excipient Components on Sugar Hydrolysis and Monosaccharides Absorption

Chan-yang Lee,Jeong-Ho Oh,Jin-Oh Chung,Chan-Su Rha,Mi-Young Park,Yong-Deog Hong,Soon-Mi Shim 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The clinical implications of FDG-PET/CT differ according to histology in advanced gastric cancer

Chon, Hong Jae,Kim, Chan,Cho, Arthur,Kim, Yoo Min,Jang, Su Jin,Kim, Bo Ok,Park, Chan Hyuk,Hyung, Woo Jin,Ahn, Joong Bae,Noh, Sung Hoon,Yun, Mijin,Rha, Sun Young unknown 2019 GASTRIC CANCER Vol.22 No.1

<P><B>Background</B></P><P>The prognostic impact of preoperative <SUP>18</SUP>F-FDG PET/CT in advanced gastric cancer (AGC) remains a matter of debate. This study aims to evaluate the prognostic impact of SUV<SUB>max</SUB> in preoperative <SUP>18</SUP>F-FDG PET/CT of AGC according to histologic subtype, with a focus on the differences between tubular adenocarcinoma and signet ring cell (SRC) carcinoma.</P><P><B>Methods</B></P><P>As a discovery set, a total of 727 AGC patients from prospective database were analyzed according to histologic subtype with Cox proportional hazard model and p-spline curves. In addition, another 173 patients from an independent institution was assessed as an external validation set.</P><P><B>Results</B></P><P>In multivariate analysis, high SUV<SUB>max</SUB> in preoperative <SUP>18</SUP>F-FDG PET/CT of AGC was negatively correlated with disease-free survival (DFS) and overall survival (OS) in patients with diffuse type (DFS: HR 2.17, <I>P</I> < 0.001; OS: HR 2.47, <I>P</I> < 0.001) or SRC histology (DFS: HR 2.26, <I>P</I> = 0.005; OS: HR 2.61, <I>P</I> = 0.003). This negative prognostic impact was not observed in patients with intestinal type or well or moderately differentiated histology. These findings have been consistently confirmed in a validation set. The p-spline curves also showed a gradual increase in log HR as SUV<SUB>max</SUB> rises only for SRC histology and for diffuse-type AGC. Finally, a novel predictive model for recurrence of AGC with diffuse type or SRC histology was generated and validated based on the preoperative SUV<SUB>max</SUB>.</P><P><B>Conclusions</B></P><P>Preoperative high SUV<SUB>max</SUB> of AGC is a poor prognostic factor in those with diffuse type or SRC histology. This study is the first to demonstrate the differential prognostic impact of preoperative PET/CT SUV<SUB>max</SUB> in AGC according to histologic subtype and provide a clue to explain previous discrepancies in the prognostic impact of preoperative PET/CT in AGC. Prospective studies are required to validate the role of preoperative SUV<SUB>max</SUB> in AGC.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1007/s10120-018-0847-5) contains supplementary material, which is available to authorized users.</P>

Production of Cyclodextrins in Ultrafiltration Membrane Reactor Containing Cyclodextrin Glycosyltransferase from Bacillus macerans

( Young Jin Son ),( Chan Su Rha ),( Yong Cheol Park ),( So Yeon Shin ),( Yoon Seung Lee ),( Jin Ho Seo ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.4

음경골절: 외과적 치료 경험 4례

고현승,나공찬,김지수,최도연,한영택 大韓泌尿器科學會 1999 Korean Journal of Urology Vol.40 No.2

Effect of Phenylalanine on Differentiation of Myoblast C1C12 and L6 Cells Into Myocytes

Hyun Woo Jeong,Jong Hyun Kim,Chan-Su Rha,Sohn Jonghee,Wangi Kim,Jonghwa Roh 건강기능식품미래포럼 2023 건강기능식품미래포럼 학술지 Vol.3 No.4

In the present study, we aimed to find any essential amino acids (EAAs) that can promote muscle protein synthesis. For this purpose, we examined how the removal of each EAA from the differentiation-inducing medium (Diff.) that is the Dulbecco’s Modified Eagle’s Medium with 2% horse serum affects the differentiation of C2C12 (mouse myoblasts) or L6 (rat myoblasts) cells into mature myocytes. This method was chosen because muscle protein synthesis is significantly enhanced during the differentiation process. This rationale was confirmed by the observation that C2C12 cells showed increased expressions of myosin heavy chain when induced to differentiate by culturing them in the Diff.. When we induced the C2C12 or L6 cells in the Diff. with each EAA depletion, we found that their differentiation rate and the expression of myogenic marker proteins (MyoD and MyoG) was blunted. This decreases were most noticeable when cultured in the Diff. with phenylalanine removed (Diff.-Phe). This effect was comparable to that of leucine. Further, the phenylalanine-removing effect could be recovered by supplementing this EAA back to the Diff.-Phe but not by adding tyrosine, indicating that the effect on differentiation is due to phenylalanine itself. These findings suggest that phenylalanine plays a role in the differentiation of myoblasts, particularly when muscle protein synthesis is enhanced, implying that phenylalanine could contribute to the promotion of muscle protein synthesis. Based on these findings, it is expected that phenylalanine, like leucine, could provide benefits in preventing the loss of muscle mass.

청도반시(Diospyros kaki Thunb. cv. Cheongdo-Bansi) 탈삽 껍질 추출물의 산화스트레스로부터 PC-12 신경세포 보호 효과

정다울(Da-Wool Jeong),조치흥(Chi Heung Cho),라찬수(Chan Su Rha),이승환(Seung Hwan Lee),김대옥(Dae-Ok Kim) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.5

본 연구에서는 떫은 감인 청도반시(Diospyros kaki Thunb. cv. Cheongdo-Bansi)를 탈삽하여 곶감으로 만드는 과정 중에 다량의 부산물로 생겨나는 감 껍질을 활용하고자 산화방지능 및 신경세포 보호능을 평가하였다. 탈삽한 감 껍질을 40% (v/v) 에탄올-물혼합용액을 사용하여 초음파 추출을 한 후에, 가열처리와 비가열처리 방법을 이용하여 각각의 추출물을 확보하였다. 가열처리한 추출물이 비가열처리로 얻어진 것에 비해서 총페놀 함량, 총플라보노이드 함량, 산화방지능에서 약 1.3-1.8배 더 높았다. 비가열처리 추출물과 비교하여, 가열처리한 것이 PC-12 신경세포의 생존율 더 높이고, 세포 내 산화스트레스를 좀 더 완화하였다. AChE와 BChE 억제능 역시 가열처리한 추출물이 비가열처리로 얻은 추출물보다 더 높았다. 이러한 결과들은 산화방지능을 보유하며 콜린가수분해효소를 억제하는 기능성 소재 발굴을 위한 탈삽 감껍질 추출물 활용 가능성을 보여준다. Astringent persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) peel with the astringency removed, which is a by-product of dried persimmon (gotgam), was investigated for its antioxidant and neuroprotective properties. A mixture of peel and 40% (v/v) aqueous ethanol was subjected to ultrasonication and then thermal and nonthermal treatments, to produce thermally-treated and nonthermally-treated persimmon peel extracts (TPE and NTPE, respectively). The total phenolic and flavonoid contents and the antioxidant capacity of TPE was approximately 1.3-1.8 times higher than those of NTPE. TPE resulted in the increased viability of neuronal PC-12 cells compared with NTPE. Furthermore, intracellular oxidative stress in PC-12 cells was more decreased by treatment with TPE than NTPE. Cholinesterases, such as acetylcholinesterase and butyrylcholinesterase, were more inhibited by treatment with TPE than NTPE. These results suggest that TPE is useful as a functional material to decrease oxidative stress in neuronal cells and to inhibit cholinesterases.

삼차신경초종의 외과적 치료

나형균,이경진,조경근,박성찬,박해관,조정기,지철,김달수,강준기,최창락,Rha, Hyung Kyun,Lee, Kyung Jin,Cho, Kyung Keun,Park, Sung Chan,Park, Hae Kwan,Chok, Jeung Ki,Chi, Chul,Kim, Dal Su,Kang, Jun Ki,Choi, Chang Rak 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.1

Objective : Trigeminal neurinomas are rare tumors that may locate in the middle fossa or posterior fossa and straddled both the middle and posterior fossa, according to their origin in the nerve complex. The aim of this study was to analyze the clinical presentation, operative approaches employed and outcome in 15 patients who were treated surgically, with special emphasis on surgical approach. Method : Between 1994 and 1998, a total of fifteen patients were histopathologically identified as neurinomas originating from the trigeminal nerve complex at the tumor clinic in the neuroscience center of the our university. Results : The surgical approach to these tumors depends on their anatomical location and tumor size. Six patients had tumors confined to the middle fossa, five patients had tumors limited to the posterior fossa, and four patients both in middle and posterior fossa components of their tumors. Nine neurinomas were removed via the conventional approach(pterional, subtemporal, suboccipital) and six were excised using skull base approach(transzygomatic subtemporal, orbitozygomatic, transpetrosal). Total resection of the tumor was possible in 10 cases. Total resection of tumor was accomplished in 83% of patients following skull base approach compared with 56% of patients following conventional approach. The surgical outcome was excellent or good in 13 cases, fair in one and, poor in one. There was no operative death. In the immediate postoperative period, aggravation of preoperative facial hypesthesia and 6th cranial nerve palsy were common. Although, these deficits were generally transient, eight patients remained with some degree of trigeminal hypesthesia, two had facial weakness, one neurotrophic keratitis, one diplopia, and one mastication difficulty. Conclusion : Surgical approach to the trigeminal neurinoma depends on the tumor location and tumor size. Skull base approach provides more complete tumor excision without increased morbidity compared to conventional approach. Surgeons have to be meticulous in order to reduce postoperative complication.

Research articles : Treatment of Atopic Dermatitis Associated with Malassezia sympodialis by Green Tea Extracts Bath Therapy: A Pilot Study

( Hyun Kyu Kim ),( Hui Kyoung Chang ),( Seok Yun Baek ),( Jin Oh Chung ),( Chan Su Rha ),( So Young Kim ),( Beom Joon Kim ),( Myeung Nam Kim ) 한국균학회 2012 Mycobiology Vol.40 No.2

Multiple treatment modalities, including topical and systemic corticosteroid and phototherapy, have been used in treatment of patients with atopic dermatitis. However, long-term corticosteroid therapy may have various adverse effects. The purpose of this study was to investigate the therapeutic efficacy and safety of bath therapy using green tea extracts for treatment of patients with atopic dermatitis. A total of four patients with atopic dermatitis were enrolled in this study. A Malassezia multiplex detection kit was used in performance of multiplex PCR on clinical isolates, which confirmed Malassezia sympodialis. Subjects underwent treatment with bath therapy using green tea extracts three times per wk for a period of 4 wk. Assessment using the scoring atopic dermatitis (SCORAD) index, the visual analogue scale for pruritus, and transepidermal water loss was performed weekly. Laboratory tests were performed before and after treatment. All patients showed marked improvement on the mean SCORAD and visual analogue scale, and a significant decrease in the mean values of serum eosinophil counts was observed after treatment. Bath therapy with green tea extract is an effective, safe, and nonsteroidal therapy for treatment of patients with atopic dermatitis associated with Malassezia sympodialis.

열수 녹차추출물이 생쥐의 체중 및 부고환 지방축적 조절에 미치는 영향

박필준(Pil Joon Park),김채욱(Chae Wook Kim),조시영(Si Young Cho),라찬수(Chan Su Rha),서대방(Dae Bang Seo),이상준(Sang Jun Lee) 한국식품과학회 2010 한국식품과학회지 Vol.42 No.1

본 연구에서는 기존 주정추출 방법에 의하여 인위적으로 카테킨 함량을 높인 녹차추출물의 효능이 아니라 음용시 사용되는 녹차가 지닌 일반적인 카테킨 함량에 의한 항비만 효능을 검토하기 위해, 열수 분무건조 방법을 적용하여 제조된 국내산 제주 열수 녹차추출물(aq-GTE)을 C57BL/6J 생쥐에 고지방 식이를 통한 비만을 유도시키며 동시에 농도를 달리하여 8주간 섭취시킨 뒤, 체중과 체내 지방함량 및 혈중 지질함량에 대한 분석을 실시하여 항비만 효능을 확인하였다. 그 결과, aq-GTE 첨가시 HFD 군간 식이 섭취량에는 차이가 없었으나 체중 증가량에 있어서는 aq-GTE에 농도 의존적으로 체중 증가량이 감소됨을 확인하였다. 또한 부고환 지방의 무게는 aq-GTE의 섭취 농도에 따라 유의하게 감소하였고, 체중 변화에 따른 비교에서도 통계적으로 유의하게 부고환 지방 무게의 감소가 확인되었다. 비만을 식이로 유도한 실험군에 나타나는 steatosis 증가 현상이 aq-GTE를 처리시 농도의존적으로 억제되어 감소하는 현상이 발생함을 관찰할 수 있었으며, 그 외에 혈액 분석을 통하여 확인할 수 있는 이상증상은 없는 것으로 확인되었다. 결론적으로 본 시험에서 사용한 aq-GTE는 C57BL/6J 생쥐에서 체중 증가와 체지방 축적 감소 및 지질대사 개선 효과를 나타내었으며, 이는 기존의 주정추출 방식으로 카테킨 함량을 높인 녹차 추출물뿐만 아니라 일반 녹차의 카테킨 함량도 충분히 그 효과를 볼 수 있다는 결과를 검증한 것이고, 향후 aq-GTE의 인체에 미치는 효과에 대한 연구가 수행되어야 할 것으로 사료된다. To obtain the best yield of the beneficial ingredients in green tea, such as catechins, green tea powder is most often prepared by ethyl alcohol extraction. However, the taste, cost and composition of ethyl alcohol extract is different from aqueous spray-dried green tea extract (aq-GTE). Specifically, aq-GTE has a better flavor, lower production costs and higher purity when compared to ethyl alcohol extract. In this study, we elucidated the effect of aq-GTE on diet-induced obesity in male C57BL/6J mice following dose-dependent oral administration of aq-GTE. After eight weeks, the body weight was reduced by 13-17% in mice fed 200 mg/kg bw aq-GTE (12.468±0.45 g; p<0.05) and 20-25% in mice fed 400 mg/kg bw aq-GTE (11.259±0.61 g; p<0.05) when compared with the high-fat diet (HFD) control group mice (14.714±0.95 g; p<0.05). The correlation between epididymal fat accumulation and body weight also decreased by approximately 26.6% (p<0.05) in mice fed a HFD with aq-GTE 400 mg/kg bw. Finally, serum parameters such as the triglyceride, glucose and cholesterol levels in the HFD groups were reduced by the aq-GTE 400 mg/kg bw diet. Analysis on glutamic-pyruvic transaminase, blood urea nitrogen and development of hepatic steatosis revealed no histologic evidence of hepatotoxicity in HFD mice fed aq-GTE. Overall, our results imply that aq-GTE is able to regulate body weight and fat accumulation in mice.

GIS와 PRISM을 이용한 고해상도 격자형 강수량 추정

신성철(Sung-Chul Shin),김맹기(Maeng-Ki Kim),서명석(Myoung-Suk Suh),나득균(Deuk-Kyun Rha),장동호(Dong-Ho Jang),김찬수(Chan-Su Kim),이우섭(Woo-Seop Lee),김연희(Yeon-Hee Kim) 한국기상학회 2008 대기 Vol.18 No.1