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Chan Yeong Kwak,Jung Hoe Kim 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Sialylation regulates the in vivo half-life of recombinant therapeutic glycoproteins, affecting their therapeutic efficacy. Levels of the precursor molecule cytidine monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) are considered a limiting factor in the sialylation of glycoproteins. Here, we show that by reducing the amount of intracellular CMP-Neu5Ac consumed for glycosphingolipid (GSL) biosynthesis, we can increase the sialylation of recombinant human erythropoietin (rhEPO) produced in CHO cells. Initially, we found that treating CHO cells with a potent inhibitor of GSL biosynthesis increases the sialylation of the rhEPO they produce. Then, we established a stable CHO cell line that produces rhEPO in the context of repression of the key GSL biosynthetic enzyme UDP-glucose ceramide glucosyltransferase (UGCG). These UGCG-depleted cells show reduced levels of gangliosides and significantly elevated levels of rhEPO sialylation. Upon further analysis of the resulting N-glycosylation pattern, we discovered that the enhanced rhEPO sialylation could be attributed to a decrease in neutral and mono-sialylated N-glycans and an increase in di-sialylated N-glycans. Our results suggest that the therapeutic efficacy of rhEPO produced in CHO cells can be improved by shunting intracellular CMP-Neu5Ac away from GSL biosynthesis and toward glycoprotein sialylation.
Chair System에 있어서 Seatback 각도에 따른 체압 및 근활성도 측정에 관한 연구
곽영찬(Yeong Chan Kwak),전경진(Keyoung Jin Chun),고철웅(Cheol Woong Ko),김수택(Soo Taek Kim),김종현(Jong Hyun Kim) 대한인간공학회 2010 대한인간공학회 학술대회논문집 Vol.2010 No.10
Sitting posture is common in daily life and approximate 75% of laborers usually work in sitting postures. Inappropriate sitting conditions for a long time could cause serious musculoskeletal disorders like lordosis, back pains. For this reason, studies on biomechanical and/or ergonomic features of human body are necessary for an optimal design process of chair system like chair, sofa, wheelchair, etc. In this Study, biomechanical tests to measure body pressure on seating area and muscle activities of human upper body by EMG sensors were performed to review optimal design parameters necessary for chair system development suitable for users.
곽찬영 ( Chan Yeong Kwak ) 한국시스템다이내믹스학회 2009 한국시스템다이내믹스 연구 Vol.10 No.4
Systemic Dynamics has been widely used as a method for studies in the areas of education, health care, and behavioral sciences. This paper discussed the SD application in nursing research by illustrating examples of published articles in nursing discipline, which can be classified into physiologic, socio-psychological, and health policy fields. It also identified the limitations of SD use in nursing and suggests strategies in order to facilitate a SD method for nursing research: 1) systematic introduction of SD in nursing research fields; 2) Interest about SD as an innovative research method; 3) researcher`s efforts to understand the SD method in academic settings.
이재은,Yeong-Chan Kwak,Eui-Bong Jeong,정화영,박성우,Hyun-Wook Jo 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.3
Equipment testing and FEM analysis are common engineering problem solving methods used by engineers. However, in the problem-solving method by FEM analysis, the engineers should use an appropriate analysis solver, assume boundary conditions, and use the correct material properties to increase the reliability of finite element (FEM) analysis. In the case of solvers, the eff orts of companies developing commercial analysis programs have greatly increased the reliability of the analysis solver, so engineers can achieve the reliability of the solver simply by selecting the correct solver for the engineering problem. However, the boundary conditions and material properties are usually based on the assumptions and experience of the engineer, but if the equipment becomes complex, uncertainty accumulates and the reliability of the analysis at the system level is greatly reduced. In particular, since there is no information when designing a product for the first time, it is difficult to expect the reliability of FEM modeling without applying an appropriate method. In the shipboard structure studied in this paper, it is difficult to predict the structural response at the system level due to the accumulation of uncertainty because the resilient mount, antenna pedestal, and radome are intricately connected and the isotropic material and anisotropic material are combined. In this paper, we applied a stepwise verification method to increase the reliability of FEM analysis of the shipboard structure. We performed experiments and FEM analysis on the shipboard structure using the method presented in this paper and found that the response of the natural frequency was consistent within about 1%. In addition, the acceleration response for all frequencies was consistent within 5%.