ULTRASONIC SENSING OF METHANOL CONCENTRATION

C. C. Sung,Y. L. Tseng,C. Y. Chen 한국소음진동공학회 2008 한국소음진동공학회 국제학술대회논문집 Vol.2008 No.7

Urinary Estrone Sulfate for Monitoring Pregnancy of Dairy Cows

Yang, C.J.,Wu, L.S.,Tseng, C.M.,Chao, M.J.,Chen, P.C.,Lin, J.H. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.9

The purpose of this study firstly was conducted to establish a radioimmunoassay (RIA) of estrone sulfate ($E_1S$), secondly to monitor the reproductive status of dairy cows using their urine samples. Urine and blood samples were collected in series within a day from four pregnant Holstein-friesian cows to evaluate the relationship between $E_1S$ levels in blood and urine with or without urinary creatinine basis. The urine was then collected biweekly from three cows in estrous and those artificially inseminated; collection from pregnant cows was made on a monthly basis. Results indicated that sensitivity for the $E_1S$ RIA was 5 pg/tube and the recovery rate was 100%. The daily urinary creatinine concentrations fluctuated within a day, but changes were slighter in midday, whereas the changes of concentrations of $E_1S$ in urine were relatively smaller. The concentrations of serum $E_1S$ during the estrous cycle were undetectable due to the limitation of assay, but the urinary $E_1S$ level could be measured with no obvious changes during the cycle. The urinary $E_1S$ levels increased remarkably around 7.7 to 8.3 ng/ml, 80 to 100 days after pregnancy but the serum $E_1S$ levels did not elevate until 120 to 150 days. The level of $E_1S$ increased gradually during pregnancy and eventually reached its peak before parturition at around 40 ng/ml and finally decreased to its basal level 2 days postparturition. During pregnancy, $E_1S$ concentrations of urine increased earlier than those in blood. The correlation coefficients between urinary and serum $E_1S$ concentration during pregnancy and postparturm were higher than those adjusted with creatinine (creatinine ratio). The concentrations of $E_1S$ in urine could be maintained unchanged for 8 days storing the samples in room temperature, which was extended to 8 days when the samples were pretreated by boiling for 30 minutes or treated with autoclave. In conclusion urinary $E_1S$ concentrations can be used directly for monitoring the pregnant status and fetal viability of dairy cows and can assist accurate confirmation of pregnancy in cows at least 80 to 100 days after insemination much earlier than by serum $E_1S$.

An Opto-Mechatronic Magnetic Field Microsensor

H. C. Chang,C. C. Hwang,C. C. Lai,C. L. Tseng 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

MEDICAL MYCOLOGY & OTHERS : CLINICAL USE OF ITRACONAZOLE ( SPORANOX , R51211 ) IN ONYCHOMYCOSES

( T . M . Jen,C . D . Tsai,Y . L . Chien,M . L . Tseng,E . M . Lee,M . T . Liu,Y . T . Chang,C . F . Chu,W . L . Jen,D . D . Lee,J . J . Shiue,Y . L . Huang ) 한국균학회 1992 MYCOLOGICAL SYMPOSIUM Vol.- No.-

Immunohistochemical Study of Steroidogenesis, Proliferation, and Hypoxia-related Proteins in Caprine Corpora Lutea during the Estrous Cycle

Chiu, C.H.,Srinivasan, R.,Tseng, T.H.,Chuang, R.F.,Wu, L.S. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The corpus luteum (CL) is a transient endocrine gland that produces progesterone, a product required for the establishment and maintenance of pregnancy. In the absence of pregnancy, the production of progesterone in the CL decreases and the structure itself regresses in size. The life span and function of the CL are regulated by complex interactions between stimulatory (luteotrophic) and inhibitory (luteolytic) mediators. When an ovum is released from a mature follicle, angiogenesis and rapid growth of follicular cells form the CL. The purpose of the present study was to determine whether steroidogenesis, proliferation, and hypoxiarelated proteins are expressed in caprine CL. The expression of proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), and hypoxia-inducible factor $1{\alpha}$ (HIF-$1{\alpha}$) were determined in caprine CL during the estrous cycle. Cytochrome P450 side chain cleavage protein did not vary significantly during the estrous cycle; however, there was an increased expression of $3{\beta}$ -hydroxysteroid dehydrogenase in the early and middle stages, which rapidly decreased in the late stage. The same observations were made with respect to steroidogenic acute regulatory protein. Variations in progesterone content and expression of PCNA, HIF-$1{\alpha}$, and VEGF were consistent with this result. Thus, the steroidogenic proteins, PCNA, HIF-$1{\alpha}$, and VEGF in caprine CL are dependent on the stage of the estrous cycle.

System Study of the Wireless Multimedia Ad-hoc Network based on IEEE 802.11g

Chung-Wei Lee,Jonathan C.L. Liu,Kun Chen,Yu-Chee Tseng,S.P. Kuo 보안공학연구지원센터 2008 International Journal of Security and Its Applicat Vol.2 No.2

The construction of a wireless multimedia ad-hoc network needs to go across the mixed environment with the indoor, the wall-penetration, and the outdoor condition. This paper presents our contribution to address the system design aspects of a multimedia-enabled network based on IEEE 802.11g ad-hoc mode. There are distinct differences between indoor and outdoor environment and penetrating the walls stressed the system limit of the 802.11g ad-hoc mode. Therefore, routing decisions should be made intelligently with the environmental respect to maximize the bandwidth support on the end-to-end paths. By investigating the experimental results of the average throughput with the 802.11g adhoc mode, we have collected the different performance characteristics among the indoor, the wall-penetration and the outdoor environment. Via the experiments and analyses, we have observed that the ad-hoc mode had the worst performance within 5 meters in indoor environments. In outdoor environments, TCP seemed to favor either short distance (e.g., 5 meters) or long distance (e.g., 25 meters). On the other hand, the best performance UDP has achieved with the distance of 10 meters. When the wall-enetration occurs, it is important that the routing nodes in the building edge areas are placed close-by within 5 meters. Given the solid evidences from the baseline experiments, we have embedded the heuristic algorithms into the routing decisions. We have thus simulated a large area of 300 meters by 300 meters with hundreds of routing nodes. After investigating over 100 (randomlygenerated) topology scenarios, the performance results indicate that our proposed scheme produces the higher-bandwidth paths for most of the cases. Even in the less-dense cases, our proposed schemes still can find the better paths with bandwidth about 30% higher than the conventional methods.

Deficiency of optineurin enhances osteoclast differentiation by attenuating the NRF2-mediated antioxidant response

Xue Peng,Hu Xiangxiang,Chang Emily,Wang Lufei,Chen Minghui,Wu Tai-Hsien,Lee Dong-Joon,Foster Brian L.,Tseng Henry C.,Ko Ching-Chang 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Abnormally increased resorption contributes to bone degenerative diseases such as Paget’s disease of bone (PDB) through unclear mechanisms. Recently, the optineurin (OPTN) gene has been implicated in PDB, and global OPTN knockout mice ( Optn −/− ) were shown to exhibit increased formation of osteoclasts (osteoclastogenesis). Growing evidence, including our own, has demonstrated that intracellular reactive oxygen species (ROS) stimulated by receptor activator of nuclear factor kappa-B ligand (RANKL) can act as signaling molecules to promote osteoclastogenesis. Here, we report that OPTN interacts with nuclear factor erythroid-derived factor 2-related factor 2 (NRF2), the master regulator of the antioxidant response, defining a pathway through which RANKL-induced ROS could be regulated for osteoclastogenesis. In this study, monocytes from Optn −/− and wild-type ( Optn +/+ ) mice were utilized to differentiate into osteoclasts, and both qRT-PCR and tartrate-resistant acid phosphatase (TRAP) staining showed that the Optn −/− monocytes exhibited enhanced osteoclastogenesis compared to the Optn +/+ cells. CellROX ® staining, qRT-PCR, and Western blotting indicated that OPTN deficiency reduced the basal expression of Nrf2 , inhibited the expression of NRF2-responsive antioxidants, and increased basal and RANKL-induced intracellular ROS levels, leading to enhanced osteoclastogenesis. Coimmunoprecipitation (co-IP) showed direct interaction, and immunofluorescence staining showed perinuclear colocalization of the OPTN-NRF2 granular structures during differentiation. Finally, curcumin and the other NRF2 activators attenuated the hyperactive osteoclastogenesis induced by OPTN deficiency. Collectively, our findings reveal a novel OPTN-mediated mechanism for regulating the NRF2-mediated antioxidant response in osteoclasts and extend the therapeutic potential of OPTN in the aging process resulting from ROS-triggered oxidative stress, which is associated with PDB and many other degenerative diseases.