Herpes Simplex Virus Type 1 Glycoprotein D Inhibits T-Cell Proliferation

Byungsuk Kwon,Soojin La,Juyang Kim,Byoung S. Kwon 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.3

Regulation of Inflammation by Bidirectional Signaling through CD137 and Its Ligand

Kwon, Byungsuk The Korean Association of Immunobiologists 2012 Immune Network Vol.12 No.5

Although the majority of research on CD137 has been directed to T cells, it is becoming clear that this molecule has distinct functions in other lineages of cells, including non-hematopoietic cells. In particular, emerging evidence suggests that the CD137-its ligand (CD137L) network involving immune cells and non-immune cells, directly or indirectly regulates inflammation in both positive and negative manners. Bidirectional signaling through both CD137 and CD137L is critical in the evolution of inflammation: 1) CD137L signaling plays an indispensible role in the activation and recruitment of neutrophils by inducing the production of proinflammatory cytokines and chemokines in hematopoietic and non-hematopoietic cells such as macrophages, endothelial cells and epithelial cells; 2) CD137 signaling in NK cells and T cells is required for their activation and can influence other cells participating in inflammation via either their production of proinflammatory cytokines or engagement of CD137L by their cell surface CD137: 3) CD137 signaling can suppress inflammation by controlling regulatory activities of dendritic cells and regulatory T cells. As recognition grows of the role of dysregulated CD137 or CD137L stimulation in inflammatory diseases, significant efforts will be needed to develop antagonists to CD137 or CD137L.

Mouse models for graft-versus-host disease

Byungsuk Kwon 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.8

Is CD137 Ligand (CD137L) Signaling a Fine Tuner of Immune Responses?

Byungsuk Kwon 대한면역학회 2015 Immune Network Vol.15 No.3

Intervention with costimulatory pathways as a therapeutic approach for graft-versus-host disease

Byungsuk Kwon 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.10

Graft-versus-host disease (GVHD) is mediated by mature donor T cells contained in the hematopoietic stem cell graft. During the development of GVHD, signaling through a variety of costimulatory receptors plays an important role in allogeneic T cell responses. Even though delivery of costimulatory signals is a prerequisite for full activation of donor T cells in the phase of their interactions with host APCs, their involvement with GVHD might occur over multiple stages. Like many other aspects of GVHD, promise of therapeutic interventions with costimulatory pathways has been gleaned from preclinical models. In this review, I summarize some of the advances in roles of costimulatory molecules in GVHD pathophysiology and discuss preclinical approaches that warrant further exploration in the clinic, focusing on novel strategies to delete pathogenic T cells.

Is CD137 Ligand (CD137L) Signaling a Fine Tuner of Immune Responses?

Kwon, Byungsuk The Korean Association of Immunobiologists 2015 Immune Network Vol.15 No.3

Now, it has been being accepted that reverse signaling through CD137 ligand (CD137L) plays an important role in vivo during hematopoiesis and in immune regulation. However, due to technical difficulty in dissecting both directional signaling events simultaneously in vivo, most biological activities caused by CD137-CD137L interactions are considered as results from signaling events of the CD137 receptor. To make the story more complex, $CD137^{-/-}$ and $CD137L^{-/-}$ mice have increased or decreased immune responses in a context-dependent manner. In this Mini review, I will try to provide a plausible explanation for how CD137L signaling is controlled during immune responses.

Immunity to melanoma mediated by 4‐1BB is associated with enhanced activity of tumour‐infiltrating lymphocytes

Ju, Seong‐,A,Lee, Sang‐,Chul,Kwon, Tae‐,Hyoung,Heo, Sook‐,Kyoung,Park, Sang‐,Min,Paek, Ha‐,Na,Suh, Jae‐,Hee,Cho, Hong Rae,Kwon, Byungsuk,Kwon, Byoung S,Kim, B Nature Publishing Group 2005 Immunology and Cell Biology Vol.83 No.4

<P>4‐1BB costimulates T cells to carry out effector functions such as eradication of established tumours. 4‐1BB (CD137) is a member of the TNF receptor family, and its triggering by either 4‐1BB ligand or antibody ligation induces T‐cell activation and growth. We analysed tumour‐infiltrating lymphocytes (TIL) in the experimental B16F10 melanoma model to determine the mechanisms involved in 4‐1BB‐mediated tumour suppression. 4‐1BB<SUP>+/+</SUP> mice survived longer than 4‐1BB<SUP>–/–</SUP> mice, and survival was further prolonged by triggering 4‐1BB with an agonistic mAb. The number of metastatic B16F10 colonies in the lung was much greater in 4‐1BB<SUP>–/–</SUP> mice than in their 4‐1BB<SUP>+/+</SUP> littermates. Administration of agonistic anti‐4‐1BB mAb increased the number of TIL in the tumour masses in the lungs of 4‐1BB<SUP>+/+</SUP> mice. The numbers of CD4<SUP>+</SUP> T, CD8<SUP>+</SUP> T and CD11b<SUP>+</SUP> TIL increased in these mice. Anti‐4‐1BB mAb induced not only CD8<SUP>+</SUP> 4‐1BB<SUP>+</SUP> T cells but also a CD8<SUP>+</SUP> IFN‐γ<SUP>+</SUP> T‐cell population. B16F10 cells from the lungs of anti‐4‐1BB‐treated mice showed enhanced expression of MHC class Ι and II antigens compared with the same cells from control IgG‐treated mice. Thus, the increase in number of CD8<SUP>+</SUP> T cells and enhanced MHC Ι and II expression in B16F10 cells that result from augmented IFN‐γ production in response to anti‐4‐1BB mAb may lead to suppression of tumour growth and metastasis.</P>

An Experimental Study on the Heat Flow of Tube Furnace by Air Flow in Tube

Byungsuk Park,Seokmin Hong,Jaewon Lee,Sangwoon Kwon,Juho Lee,Changhwa Lee 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

In this paper, a basic study was conducted to observe the temperature inside the tube according to the heating temperature of the tube furnace. In a tube furnace, a tube is inserted, and the air space outside the tube is heated to increase the temperature of the gas inside the tube through conduction of the tube. Tube furnaces are widely used in research to capture volatile nuclides. In this case, a volatile nuclide capturing filter is inserted inside the tube, and an appropriate temperature is required to capture it. Since the tube furnace heats the air space outside the tube to the target temperature, a difference from the temperature inside the tube occurs. In particular, if a flow of gas occurs inside the tube, a larger temperature difference may occur. In order to confirm this temperature difference, an experimental device was constructed, and basic data was produced through several experiments. The following studies were conducted to produce data. First, the temperature of the air layer of the heating unit and the temperature inside the tube were measured in real time in the absence of gas flow inside the tube. Second, the temperature of the air layer of the heating unit and the temperature inside the tube were measured in real time while air having a certain temperature was flowing inside the tube. As a result of the experiment, when there is no flow inside the tube, when the heating target temperature is low, the temperature inside the tube is significantly lower than the target temperature, and when the target temperature is high, the temperature inside the tube approaches the target temperature. It was found that when there is about 20°C air flow inside the tube, the temperature inside the tube is significantly lowered even if the heating target temperature is high. In the future, additional research on changing the temperature of the gas flowing inside the tube will be conducted, and the results of this study are expected to greatly contribute to the design of a tube furnace that captures volatile nuclides.

An Experimental Verification Study on the Heat Flow Analysis Results of a Tube Furnace

Byungsuk Park,Sangwoon Kwon,Juho Lee,Changhwa Lee 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.2

In general, systems are developed by repeatedly performing the processes of design, analysis, manufacturing, and performance testing. In particular, systems with temperature, pressure, and flow rate often utilize computational fluid dynamics tools at the design stage. In this paper, we aim to verify the reliability of the analysis results of Solidworks Flow Simulation, which is widely used in heat flow analysis at the design stage. A tube furnace was manufactured, various experiments were performed, and a study was conducted to compare the analysis results. The details of the experiment are as follows. First, an experiment was conducted in which the heater was heated to 900°C without insulating the exposed part of the tube. The detailed contents of the experiment are as follows; - Heating heater and measuring temperature without supplying flow inside the tube, - Tube flow supply (25°C, 15 lpm air) and heater heating/temperature measurement. Second, an experiment was performed in which the exposed part of the tube was insulated (thickness 50 mm) and the heater was heated to 900°C. The detailed contents of the experiment are as follows; - Insulate the outside of the tube except for the flanges at both ends of the tube, and heat the heater and measure the temperature without supplying flow inside the tube. - Insulate the outside of the tube except for the flanges at both ends of the tube, supply flow rate inside the tube (25°C, 15 lpm air) and measure heater heating/temperature. - Insulate the flange of the flow supply section, heat the heater and measure temperature without supplying flow inside the tube. - Insulate the flange of the flow supply section, heat the supply air (277°C, 15 lpm) and measure the temperature using a heating gun without heating the heater. - Insulate the flange of the flow supply section, supply heated air (277°C, 15 lpm) and measure heater heating/temperature. - Insulate the flange of the flow supply section and measure temperature according to heater heating (900°C) and supply temperature (25°C, 277°C 15 lpm). The following results were derived from the experimental and analysis results. - When the exposed part of the tube is insulated, the temperature inside the tube increases and the steady-state power decreases compared to non-insulated. - In areas with insulation, the temperature error between experiment and analysis results is not large. - When flow rate is supplied, there is a large temperature error in experiment and analysis results. - The temperature change after the center of the heater is not large for a temperature change of 15 lpm flow rate. From these results, it can be seen that Solidworks Flow Simulation has a significant difference from the experimental results when there is a flow rate in the tube. This was thought to be because the flow rate acts as a disturbance, and this cannot be sufficiently accounted for in the analysis. In the future, we plan to check whether there is a way to solve this problem.

앵커 긴장력 변화에 따른 비탈면 유지관리 연구

박병석 ( Byungsuk Park ),김우석 ( Wooseok Kim ),황성필 ( Sungpil Hwang ),권오일 ( Oil Kwon ) 대한지질공학회 2020 지질공학 Vol.30 No.4

비탈면의 안정성 측면에서 파괴형태에 따라 예상 파괴면에서의 지반 전단력과 앵커보강에 의한 저항력은 중요한 요소로 작용할 수 있다. 또한, 앵커가 보강된 비탈면에서는 지압판이 설치된 지반의 풍화, 침하 및 불완전하게 방청 처리된 강연선의 부식 등으로 인하여 앵커축력이 변할 수 있다. 그러나 앵커 축력의 변화로 인해 국부적으로 앵커의 저항력을 상실한 경우에는 앵커가 저항력을 발휘하지 못하게 되어 주변 앵커로 외력이 전가되는 경향이 있으며, 이에 따라 주변앵커의 긴장력을 증가시키게 된다. 이로 인해 전체 비탈면의 안정성에 문제가 발생하게 되므로 주의가 요구된다. 따라서 본 연구에서는 비탈면에 설치되어 있는 앵커의 긴장력 변화경향을 모니터링하고 이를 고려한 비탈면 전체의 외적 안정성을 유추하는 방법을 제안하고자 하였다. The ground shear force at the expected failure surface and resistance force due to reinforced anchor can act as important factors according to a failure type from the stability viewpoint at a slope. Furthermore, the anchor’s axial force may vary at an anchor-reinforced slope due to ground weathering, settlement, and corrosion in the incompletely anti-corrosion treated steel wire strand at a ground where the bearing plate is installed. However, in case that the resistance force of the anchor is locally lost due to the variation of the anchor’s axial force, the resistance force may not play the role so that the external force tends to be transferred to the surrounding anchors, causing an increase in the tensile force in the surrounding anchors. Accordingly, a stability problem at the entire slope may occur, which requires much attention. Thus, this study proposed a method to monitor a variation trend of the tensile force of anchors installed at a slope and infer the external stability at the entire slope considering the monitoring result.