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( Bo Hye Kim ),( Hee Kyung Kim ),( Hye Jeong Cho ),( So Dam Hong ),( Jae Kyoung Shin ),( Hee Jin Hong ),( Sae Ahm Kim ),( Hak Su Kim ),( Suk Pyo Shin ),( Ha Na Park ),( Seong Gyu Hwang ),( Kyu Sung Ri 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Pyogenic liver abscess is the most common type of visceral abscess with a mortality rate of 10% to 25%. The mortality of liver abscess markedly decreased due to the improvement of diagnostic methods, antibiotics and other treatment modalities. We describe the clinical feature, changing trends of etiology and poor prognostic factors of pyogenic liver abscess during the recent 2 decades. Methods: We retrospectively reviewed the medical records of patients with pyogenic liver abscess who were treated in CHA Bundang Medical Center between 1996 and 2014. The period was divided into period 1 (1996-2004) and period 2 (2005-2014). We compare these two groups in age, etiology, etc. Results: 148 patients were indentifi ed. 60 patients were included period 1 and 86 patients were included period 2. There were no signifi cant interval changes in symptom, sign, number and size of abscess and laboratory fi nding between the two periods. The average age increased in period 2 (aged 62. 7) relative to period 1 (aged 57. 2) (P=0. 001). Klebsiella pneumoniae was the most common pathogen both two periods. The most common infection route was biliary tract (45. 5%) in period 1 and cyptogenic cause (53. 8%) in period 2 (P=0. 042). Interestingly, the percentage of hepatobiliary malignacy in etiology increased from 4. 5% in Period 1 to 10. 2% in period 2. (P= 0. 03) Liver abscess originated from hepatobiliary problem showed a higher recurrent rate (P=0. 018). Conclusions:The average age of patients with pyogenic liver abscess has increased substantially and the predominant pathogen originates from the biliary tract because biliary procedures has increased. Hepatobiliary malignancy is the major risk factor and poor prognostic factor for pyogenic liver abscess. Therefore early diagnosis and close observation is important for patients with biliary problem or hepatobiliary malignancy.
Prenatal diagnosis of a 7q21.13q22.1 deletion detected using high-resolution microarray
( Kyoung Bo Kim ),( Jung Sook Ha ),( So Jin Shin ),( Chun Soo Kim ),( Jin Gon Bae ) 대한산부인과학회 2014 Obstetrics & Gynecology Science Vol.57 No.4
We report a case of de novo 7q interstitial deletion detected by conventional karyotyping and by microarray of amniotic fluid sampled during the prenatal period. A 32-year-old pregnant woman was evaluated at our hospital following detection of increased nuchal translucency at 12 weeks and 5 days of gestation. Conventional karyotyping revealed 46,XX,del(7)(q21q22) in 20 interphase mitotic cells, and high-resolution microarray revealed 12.8 Mb (90,625,014·103,430,901) deletion in the region 7q21.13q22.1. Both parents had normal karyotypes. After birth, the neonate displayed several anomalies, including palatine cleft, upslanted and wide palpebral fissure, low-set ears, micrognathia, microcephaly, ventriculomegaly, subglottic tracheal stenosis, hearing loss, and hand/foot deformities, including brachydactyly, polydactyly, and cutaneous syndactyly. This case study helps explain the phenotype-genotype relationship in patients with 7q21.13q22.1 deletion.
Ha Su Min,Kim Hak Hee,Kang Eunhee,Seo Bo Kyoung,Choi Nami,Kim Tae Hee,Ku You Jin,Ye Jong Chul 대한영상의학회 2022 대한영상의학회지 Vol.83 No.2
Purpose To develop a denoising convolutional neural network-based image processing technique and investigate its efficacy in diagnosing breast cancer using low-dose mammography imaging. Materials and Methods A total of 6 breast radiologists were included in this prospective study. All radiologists independently evaluated low-dose images for lesion detection and rated them for diagnostic quality using a qualitative scale. After application of the denoising network, the same radiologists evaluated lesion detectability and image quality. For clinical application, a consensus on lesion type and localization on preoperative mammographic examinations of breast cancer patients was reached after discussion. Thereafter, coded low-dose, reconstructed full-dose, and full-dose images were presented and assessed in a random order. Results Lesions on 40% reconstructed full-dose images were better perceived when compared with low-dose images of mastectomy specimens as a reference. In clinical application, as compared to 40% reconstructed images, higher values were given on full-dose images for resolution (p < 0.001); diagnostic quality for calcifications (p < 0.001); and for masses, asymmetry, or architectural distortion (p = 0.037). The 40% reconstructed images showed comparable values to 100% full-dose images for overall quality (p = 0.547), lesion visibility (p = 0.120), and contrast (p = 0.083), without significant differences. Conclusion Effective denoising and image reconstruction processing techniques can enable breast cancer diagnosis with substantial radiation dose reduction.
( Kyoung Woon Kim ),( Yoo Jin Shin ),( Bo-mi Kim ),( Sheng Cui ),( Eun Jeong Ko ),( Sun Woo Lim ),( Chul Woo Yang ),( Byung Ha Chung ) 대한신장학회 2021 Kidney Research and Clinical Practice Vol.40 No.4
Background: Endothelial cell (EC) dysfunction is a frequent feature in patients with end-stage renal disease (ESRD). The aim of this study was to generate human induced pluripotent stem cells, differentiate ECs (hiPSC-ECs) from patients with ESRD, and appraise the usefulness of hiPSC-ECs as a model to investigate EC dysfunction. Methods: We generated hiPSCs using peripheral blood mononuclear cells (PBMCs) isolated from three patients with ESRD and three healthy controls (HCs). Next, we differentiated hiPSC-ECs using the generated hiPSCs and assessed the expression of endothelial markers by immunofluorescence. The differentiation efficacy, EC dysfunction, and molecular signatures of EC-related genes based on microarray analysis were compared between the ESRD and HC groups. Results: In both groups, hiPSCs and hiPSC-ECs were successfully obtained based on induced pluripotent stem cell or EC marker expression in immunofluorescence and flow cytometry. However, the efficiency of differentiation of ECs from hiPSCs was lower in the ESRD-hiPSCs than in the HC-hiPSCs. In addition, unlike HC-hiPSC-ECs, ESRD-hiPSC-ECs failed to form interconnecting branching point networks in an in vitro tube formation assay. During microarray analysis, transcripts associated with oxidative stress and inflammation were upregulated and transcripts associated with vascular development and basement membrane extracellular matrix components were downregulated in ESRD-hiPSC-ECs relative to in HC-hiPSC-ECs. Conclusion: ESRD-hiPSC-ECs showed a greater level of EC dysfunction than HC-hiPSC-ECs did based on functional assay results and molecular profiles. hiPSC-ECs may be used as a disease model to investigate the pathophysiology of EC dysfunction in ESRD.
Ahn, kyoung-Jin,Nam, Bo-Hye,Kim, Young-Ok,Kang, Jung-Ha,Kim, Bong-Seok,Jee, Young-Ju,Lee, Sang-Jun The Korean Society of Fisheries and Aquatic Scienc 2007 Fisheries and Aquatic Sciences Vol.10 No.1
The induction of cellular and humoral immunity and cytokine gene expression by synthetic CpG oligodexoynucleotides (CpG-ODNs) has not been investigated systematically in olive flounder Paralichthys olivaceus in vivo. We optimized the proper concentration of CpG-ODNs using an in vitro assay for the superoxide anion $(O_2^-)$. CpG-ODNs induced $O_2^-$ and nitric oxide (NO) production, lysozyme activity, and the proinflammatory cytokine gene expression of $IL-1{\beta}$ and $TNF-{\alpha}$ in olive flounder significantly in vivo, whereas non-CpG-ODNs did not produce these effects or produced them to a lesser extent. This implied that CpG-ODNs could stimulate cellular and humoral immunity and cytokine gene expression in olive flounder. This is the first evidence of NO production and the first study on the mRNA expression of the proinflammatory cytokine genes $IL-1{\beta}$ and $TNF-{\alpha}$ in olive flounder in response to CpG-ODNs. Comparison of the variation in NO production and lysozyme activity to that of other studies led us to postulate that a group-specific difference exists in the immune responses of olive flounder against CpG-ODNs. Furthermore, the detailed immunostimulatory spectrum of CpG-ODNs in olive flounder could be a useful index with which to analyze the effect of CpG-ODNs against the challenge test prior to field applications.
B cell-associated immune profiles in patients with end-stage renal disease (ESRD)
Kim, Kyoung Woon,Chung, Byung Ha,Jeon, Eun Joo,Kim, Bo-Mi,Choi, Bum Soon,Park, Cheol Whee,Kim, Yong-Soo,Cho, Seok-Goo,Cho, Mi-La,Yang, Chul Woo Korean Society for Biochemistry and Molecular Bion 2012 Experimental and molecular medicine Vol.44 No.8
Most of the previous studies on immune dysregulation in end-stage renal disease (ESRD) have focused on T cell immunity. We investigated B cell subpopulations in ESRD patients and the effect of hemodialysis (HD) on B cell-associated immune profiles in these patients. Forty-four ESRD [maintenance HD patients (n = 27) and pre-dialysis patients (n = 17)] and 27 healthy volunteers were included in this study. We determined the percentage of B cell subtypes, such as mature and immature B cells, memory B cells, and interleukin (IL)-$10^+$ cells, as well as B cell-producing cytokines (IL-10, IL-4 and IL-21) by florescent activated cell sorting (FACS). B cell-associated gene expression was examined using real-time PCR and B cell producing cytokines (IL-10, IL-4 and IL-21) were determined using an enzyme-linked immunosorbent assay (ELISA). The percentage of total B cells and mature B cells did not differ significantly among the three groups. The percentages of memory B cells were significantly higher in the pre-dialysis group than in the HD group (P<0.01), but the percentage of immature B cells was significantly lower in the pre-dialysis group than in the other groups. The percentages of IL-10-expressing cells that were $CD19^+$ or immature B cells did not differ significantly (P>0.05) between the two subgroups within the ESRD group, but the serum IL-10 concentration was significantly lower in the pre-dialysis group (P<0.01). The results of this study demonstrate significantly altered B cell-associated immunity. Specifically, an imbalance of immature and memory B cells in ESRD patients was observed, with this finding predominating in pre-dialysis patients.
Yong Bo Park,김광하,Kyungbin Kim,Tae Kyoung Ha,Guk Bin Park,Young Min Kwak 대한상부위장관ㆍ헬리코박터학회 2021 Korean Journal of Helicobacter Upper Gastrointesti Vol.21 No.1
Gastric adenocarcinoma of fundic gland type is known to originate from the gastric mucosa of the fundic gland region without intestinal metaplasia. It is difficult to detect during endoscopy and diagnose histopathologically. The development of immunohistochemistry has enabled the diagnosis of gastric adenocarcinoma of fundic gland type by gastric phenotype. A 34-year-old man visited us for treatment of a 5 cm-sized low-grade dysplasia in the gastric fundus which was incidentally found during a health check-up endoscopy. Endoscopic submucosal dissection was performed successfully, and the final histopathology showed gastric adenocarcinoma of fundic gland type. Herein, we report a rare case of gastric adenocarcinoma of fundic gland type and its endoscopic and histopathologic findings.