C6 glia 세포에서 유도성 Nitric Oxide Synthase 유전자 발현조절에 관한 연구

배진영,허강민,배소현,박지선,이충재,이재흔,석정호 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

To investigate transcriptional regulation of iNOS gene by LPS and cytokines, the production of NO, expression of iNOS mRNA and protein, binding activity of nuclear factor-kappa B(NF-kB), and promoter activity of iNOS gene were examined in rat C6 glial cells. LPS, interferon-gamma(IFN-γ), and tumor necrosis factor-alpha (TNF-α) stimulated the production of NO, which was increased synergistically by co-treatment. By the treatment of LPS, iNOS mRNA expression was initiated at 1 h, markedly increased by 3 h, and decreased gradually afterward. iNOS mRNA expression was markedly enhanced by mixture of LPS, IFN-γ and TNF-α. iNOS protein synthesis was increased by the treatment of mixture LPS and cytokine mixture. Treatment of LPS stimulated NF-kB activation, and the activation reached to the maximum level at 30 min, and the treatment of mixture of LPS and cytokines increased the activation. To determine the effect of NF-kB binding activity on iNOS promoter activation, CAT assay was performed. iNOS promoter activity was increased by the treatment with LPS for 5.5 h, and further increased by the combined treatment with LPS and cytokines. These results suggest that NF-kB activation by LPS and cytokines may play a significant role in the induction of the iNOS gene.

노인에서의 외과 환자에 대한 임상적 연구

배진선,강민규,송인상 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

378 patients over 65 years old were reviewed, who were admitted during the period from June. 1. 1985 to December 31. 1988 in the Department of Surgery, Chungnam National University Hospital. The results were as followings: 1. The rate of patients over 65 years to all who were admitted during same period was 11.3%. 2. The number of male patients was 183, that of female 195, the overall ratio of male to female being 1:1.07. In benign diseases, that was 1:0.95, and in malignant diseases 1:1.26. 3. Hemoglobin value at admission was below 10.0gm% in 7.5% of patients with benign diseases and in 15.0% of patients with malignant diseases. 4. Serum albumin at admission was below 3.5gm% in 60% of patients with benign diseases and in 50% of patients with malignant diseases. 5. As to the distribution of benign diseases, disease in biliary system was most frequent(33%), followed by appendicitis(25%), ileus and trauma in jejunum and ileum(13%), and peptic ulcer(6%) in the order of frequency. 6. As to the distribution of malignant diseases, stomach cancer(51%) was most frequent, followed by colorectal cancer(25%), and hepatobiliary cancer(10%) in the order of frequency. 7. 47% of patients with benign diseases and 44% of that with malignant diseases had associated diseases, among which hepertension was most frequent, followed by lung disease, hearth disease, diabetes mellitus, liver disease and renal disease in the order of frequency. 8. Complications were most prevalent in the patients with hepatobiliary diseases, followed by appendicitis and colorectal cancer, and among complications, would infection was most frequent, followed by urinary tract infection and respiratory infection in the order of freguency. In the patients with colorectal cancer, mechanical lieus was second frequent complication after wound infection. 9. The mortality rate was highest in the patient with colorectal cancer(22.2%), followed by in those with benign biliary disease(10%), overall mortality rate being 4.3%. 10. Major causes of deaths were sepsis, respiratory failure, bile peritonitis and renal failure.

胃癌患者의 臨床的考察 : Clinical Study and Review of Literatures

裵振善,河基正 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

The stomach cancer is the most common malignant tumor in men and 2nd or 3rd most common malignant tumor in female in Korea. Retrospective clinical study was performed among 103 patients who were admitted under the diagnosis of stomach cancer during the past 5 years and 6 months from Jan. 1976 to June 1981 to the Dept. of Surgery, School of Medicine, Chung-nam National University Hospital. The following results were obtained; 1. Male predominated the female by 3 : 1. 2. The peak incidence of ages was 6th decade mounting to 43.9%, followed by 5th decade. 3. The predominating symptom was epigastric pain or discomfort by 77.7%. 4. The mass was palpated in 43.7% of patients and epigastric tenderness was revealed in 66.0%. 5. Anacidity or hypoacidity was found in 84.4% and only in 2.2%, the acidity was above normal. 6. Through the radiological study, only 3.6% was revealed normal. 7. Through endoscopy, tumor or ulcer was found in 97.4%. 8. Operations were undertaken in 73.8% of patients, among those, subtotal gastrectomy was in 60.5%, total gastrectomy in 6.6%. 9. Adenocarcinoma was predominated histologically in 97.4%. 10. Postoperative complications were found in 6.3%, among them, wound infections were most frequent(25%), and postoperative mortality was 2.6%. 11. Regarding to clinical diagnosis, 9.7% of patients was proved false positive.

갑상선암의 임상적 고찰

배진선,설지영 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Thyriod carrinoma is rare, representing approximately 1% of all malignancies but account for 90% of all endocrine malignancies. Thyroid carcinoma has a wide spectrum of biologic activity varying from nearly innocuous to very lethal malignancies. The primary treatment for thyroid carcinoma is surgical excision. But the extent of resection, indications for regional lymph node dissection, and the postoperative follow up of patients are the most controversial aspects of management. To formulate a valid rationale for the diagnosis and treatment of the thyroid cancer, a retrospective study of the thyroid cancer treated at the Department of Surgery, Chungnam National University Hospital from January, 1986 to October, 1991 was conducted. The results were as follows ; 1)By the pathological classification, the papillary carcinoma was the most common type and its incidence was 81.1% 2)Female was predminated over male by the ratio of 6.5:1 and most common age incidence was noted from fourth decade to sixth decade, that was 68.9%. 3)The most common duration of illness between the appearance of the symptoms and the treatment was 3 months, which was 31.1%. 4)The most common symptom was the palpable mass at the anterior portion of the neck. 5)Most cases of the thyroid cancer were appeared as cold nodule in the ^99m Tc-Thyroid scan, which was 98.9% in incidence. 6)The right lobe(50%)was the more common site of the thyroid cancer in comparison to the left lobe(28.9%). 7)The incidence of thyroid cancer located in the gland without extrathyroidal extension was 48.9%, and that of the metastasis to the cervical lymph nodes was 34.4% 8)The common surgical procedures of the thyriod cancer were lobectomy which incidence was 51.1%, total thyroidectomy 15.6%, near-total thyroidectomy 10.0% and lobectomy with isthmusectomy 5.6% in order respecitively. And the neck dissection was performed in 16 cases(17.7%) 9) The most common postoperative complications were transient hypoparathyroidism(11.1%), and followed by transient unilateral recurrent laryngeal nerve paralysis(10.0%), hematoma(5.6%). bilateral recurrent laryngeal nerve paralysis and permanent hypoparathyroidism.

위암환자에서 수용성 Intercellular Adhesion Molecule-1의 의의

배진선,송인상,김완식 한국의학사 1999 診斷과治療 Vol.19 No.8

血淸 CEA値에 關한 臨床的 硏究

裵振善,尹必逵 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.2

The serum carcinoernbryonic antigen (CEA) values as an indicator of colorectal carcinoma has been accepted, however the CEA value is increased not only in colorectal cancer but in many other conditions, such as chronic pulmonary disease, heavy smoker, inflammatory bowel disease, pancreatitis, liver diseases, biliary tract disease, breast disease, ovarian disease, uterine disease, cervix disease etc. Although it may not be suitable to screening test, nevertheless its serial check is very valuable for early detection of recurrence after removal of tumor by the increase of serum CEA value during postoperative follow-up. Serum CEA values were checked by the patients of peptic ulcer, injury and other non-malignant disease, stomach cancer, breast cancer, and colorectal cancer treated from March 1982 to October 1982 in the Department of General Surgery, Chungnam National University Hospital. The following results were obtained; 1. The level in control was 2.5±1.9 ng/ml. 2. By the patients of stomach cancer, the preoperative level being 39.7±74.1 ng/ml, the postoperative level was 31.4±62.8 ng/ml. 3. By the patients of breast cancer, the preoperative level being 13.3±7.8 ng/ml, the postoperative level was 11.0±8.3 ng/ml. 4. By the patients of colorectal cancer, the preoperative level being 39.0±61.8 ng/ml, whereby the postoperative level was 21.3±35.5 ng/ml.

大腸 肛門癌의 臨床的 硏究

裵振善,朴仁根 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

A clinical study was undertaken on 172 patients of colorectal and anal cancer who was admitted during 8 years from Jan. 1978 to Dec. 1985 in the Department of Surgery, Chungnam National University Hospital. The results were as followings; 1. In male, it was slight more prevalent than in female, the incidence ratio of male to female being 1.1:1. 2. As to the age distribution, the prevalent group was 10 years earlier compared with Western countries, i,e. 50 years (28.4%), followed by 40 years (25.3%), 60 years (17.9%), and 30 years (11.7%) in order of frequency. 3. The most prevalent site of tumor was rectum(55.7%), followed by sigmoid colon (8.9%), ascending colon (7.0%); 63.9% of tumors was located distal to the sigmoid. 4. The most frequent symptom was melena (55.6%), followed by abdominal pain (44.4%), change of bowel habit (36.1%) etc. 5. As to the duration of symptoms, 61% of patients was within 6 months, 80% within 1 year. 6. Hemoglobin on admission was under 10.0mg% in 26% of patients. 7. The diagnostic methods were barium enema, digital rectal examination, fiberoptic colonoscopy and sigmoidoscopy. 8. Resection was undertaken in 75% of patients; Miles' operation was most frequent (25.0%), followed by anterior resection (20.8%), and right hemicolectomy (16.7%). 9. 30.6% of patients had cancer metastasis; lymph node metastasis being most frequent (22.2%), followed by lilver metastasis (9.7%), bone metastasis (2.8%), and lung metastasis (1.4%). 10. Histologic examination revealed adenocarcinoma (95.7%), squamous cell carcinoma (2.1%), and malignant lymphoma (2.1%). 11. 33% of patients had complications, in which wound infection was most frequent (66.7%), followed by intestinal obstruction (20.8%), bladder dysfunction (16.7%), and fecal fistula (16.7%). 12. 92% of patients revealed good or excellent result, the mortality was 1.4%.

Chlorella의 生長에 미치는 카드뮴 및 아연의 影響

裵啓宣,崔震浹,孫賢秀 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

The study was carried out to examine effect of cadmium and zinc on the growth of Chlorella. The results obtained were as follows. 1. The chlorella cells damaged by heavy metals showed yellowish to milk-white symptoms at initial stage and died at later stage. 2. The cadmium has more inhibitory effect than on the turbidity cell number, chlorophyll contents and protein contents. 3. The chlorella cells were damaged more remarkably by the higher concentration of cadmium and zinc. 4. In conclusion, the minimum concentration of heavy metals to effect on the growth rate of chlorella cells were 2 ppm cadmium and 30 ppm of zinc.

甲狀腺의 臨床的 硏究

裴振善 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

Thyroid gland is the most frequent endocrine organ which requires operation in surgery department. Among 95 patients whe were admitted for operation due to thyroid disease during 3.5years from Jul. 1. 1980 through Dec. 31. 1983 in the Department of Surgery, Chungnam National University Hospital, 18 patients were proven as thyroid cancer. The results about cancer were as follows; 1. The sex ratio of male to female was 1:8. 2. Age distribution in female was 37.5% in 3rd decade and 43.8% in 4th decade. 3. In all patients, mass could be palpable, on the contrary in 78% of patients there w subjective symptom, only 2 patients had easy fatigability, 2 patients palpitation, I patient slight dyspnea. 4. About to the location, the ratio of right to the left was almost equal, in one case it located in the isthmus. 5. Thyroid function test was within normal limit only but one case. 6. 37 year old female patient had follicular cancer in the left lobe and papillary cancer in the right lobe. 7. As to the method of operation, lobectomy was performed in 7 cases, lobectomy and isthmectomy in 7 cases, subtotal thyroidectomy in 3 cases, total thyroidectomy in 1 case. 8. About to the pathological findings, papillary cancer was found in 9 cases, papillary-follicular cancer in 2 cases, follicular cancer in 6 cases. 9. As to the result, in 89% of patients, there was no complication, but one case had hoarseness.

非結石性 膽囊炎에 關한 臨床的 硏究

裵振善,睦愚均 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

A clinical study was undertaken on the 35 cases of acalculous cholecystitis among 392 cases operated for the biliary disease during the period from. January 1980 through June 1987 admitted in the department of surgery Chungnam National University Hospital. The results obtained were as followings; 1. The incidence of acalculous cholecystitis was 8.9% and extragallbladder biliary stone 3.1% among all cholecystitis. 2. The incidence of acalculous cholecystitis was slight more prevalent in female in contrast to many reports, i. e. the ratio of male to female was 1 : 1.2. 3. In regard of the age distribution, 5th decade was most prevalent, followed by 7th, 4th, & 6th decade in order of frequency; 91.4% of all patients were older than 30 years. 4. Right upper quadrant pain (74%) was most frequent clinical symptom, followed by nausea and vomiting (57%), fever & chillness (54%), & epigastric pain (37%) in order of frequency. 5. 37% of patiients was within one week of duration of symptoms, 14% between 1∼2 weeks, & 63% of patients within 4 weeks, 6. Right upper quadrant tenderness (77%) was most frequent positive physical finding, followed by jaundice (26%), & right upper quadrant mass (20%). 7. The elevated SGPT(74%) was most frequent positive laboratory finding, followed by elevated SGOT (69%), elevated serum bilirubin above 1. 5mg% (66%), above 3.0mg% (40%), leukocytosis (37%) & elevated alkaline phosphatase (37%). 8. Ileus on simple abdomen (60%) was most frequent finding as diagnostic measures, nonvisualisation of gallbladder in oral cholecystography or cholescintigraphy (17%) ; in most cases, the findings were nonspecific, therefore it can be said that clinical findings were most important in the diagnosis of acalculous cholecystitis. 9. Preoperatively, it was diagnosed as chronic cholecystitis (37%), empyema gallbladder (11%), gallbladder stone (11%), choledocholithiasis (9%), & acalculous cholecystitis (6%). i. e. the preoperative diagnostic accuracy was very low. 10. The most frequent operation underwent was cholecystectomy with or without T-tube drainage (86%), followed by cholecytostomy (9%), & choledochojejunostomy (5. 7%). 11. Bile culture was performed in 14 cases, 79% being positive, among them E. coli was most frequent (29%), followed by Klebsiella (21%), Proteus mirabilis, Streptococcus intermidius, & Streptococcus epidermidis. 12. Regarding etiologic and predisposing factors, ascaris was found in 14% of cases, Clonorchis sinensis (9%), & common bile duct cancer (9%), gallblader cancer (9%), pancreas head cancer (6%), & unclear(37%). 13. Postoperative complications were developed in 8 cases (23%), among them wound infections were most frequent (4 cases), followed by wound dehiscence (1 case), intraabdominal abscess (1 ,case), biliary fistula (1 case), & cholangitis (1 case).