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Angel Chao,Yi-Hao Lin,Lan-Yan Yang,Ren-Chin Wu,Wei-Yang Chang,Pi-Yueh Chang,Shih-Cheng Chang,Chiao-Yun Lin,Huei-Jean Huang,Cheng-Tao Lin,Hung-Hsueh Chou,Kuan-Gen Huang,Wen-Ling Kuo,Ting-Chang Chang,Ch 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.3
Objective: The characteristics of patients with metachronous breast and ovarian malignancies and the pathogenic role of BRCA1/2 mutations remain poorly understood. We investigated these issues through a review of hospital records and nationwide Taiwanese registry data, followed by BRCA1/2 mutation analysis in hospital-based cases. Methods: We retrospectively retrieved consecutive clinical records of Taiwanese patients who presented with these malignancies to our hospital between 2001 and 2017. We also collected information from the Data Science Center of the Taiwan Cancer Registry (TCR) between 2007 and 2015. Next-generation sequencing and multiplex ligation-dependent probe amplification were used to identify BRCA1/2 mutations and large genomic rearrangements, respectively. When BRCA1/2 mutations were identified in index cases, pedigrees were reconstructed and genetic testing was offered to family members. Results: A total of 12,769 patients with breast cancer and 1,537 with ovarian cancer were retrieved from our hospital records. Of them, 28 had metachronous breast and ovarian malignancies. We also identified 113 cases from the TCR dataset. Eighteen hospital-based cases underwent BRCA1/2 sequencing and germline pathogenic mutations were detected in 7 patients (38.9%, 5 in BRCA1 and 2 in BRCA2). All BRCA1/2 mutation carriers had ovarian high-grade serous carcinomas. Of the 12 patients who were alive at the time of analysis, 5 were BRCA1/2 mutation carriers. All of them had family members with BRCA1/2-associated malignancies. Conclusions: Our results provide pilot evidence that BRCA1/2 mutations are common in Taiwanese patients with metachronous breast and ovarian malignancies, supporting the clinical utility of genetic counseling.
Yang, Angel,Park, Jong-Hyouk,Abd El-Aty, A.M.,Choi, Jeong-Heui,Oh, Jae-Ho,Do, Jung-Ah,Kwon, Kisung,Shim, Ki-Hoon,Choi, Ok-Ja,Shim, Jae-Han Elsevier 2012 FOOD CONTROL Vol.28 No.1
<P><B>Abstract</B></P><P>The principal objective of this study was to investigate the effect of household processing, including washing and cooking on pesticide residue levels in various food samples. For this study, 31 food materials were selected and 44 pesticide residues were monitored using the “quick, easy, cheap, effective, rugged, and safe” QuEChERS extraction-based and liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods. Eight pesticides, including acetamiprid, azoxystrobin, fenobucarb, fosthiazate, iprobenfos, lufenuron, propiconazole, and trifloxystrobin were detected in nine food samples including colored rice, glutinous rice (white rice), glutinous rice (unpolished rice), green chili, ginger, butterbur, chinamul, spinach, and perilla leaf. Results indicated that residue levels in positive food commodities declined substantially following washing and cooking. However, the residual level of acetamiprid increased in green chilis after boiling and stir-frying. In sum, household processing (washing and cooking) tended to substantially reduce or eliminate pesticide residues in a synergistic manner. These applications are necessary to protect consumers from the negative health effects of pesticide residues detected in food commodities.</P> <P><B>Highlights</B></P><P>► The effects of household processing on pesticide residue levels were investigated. ► Residue levels were substantially declined following washing and cooking. ► The household processing tend to remove the residues in a synergistic manner.</P>
An-Shine Chao,Angel Chao,Chyong-Huey Lai,Chiao-Yun Lin,Lan-Yan Yang,Shih-Cheng Chang,Ren-Chin Wu 대한부인종양학회 2024 Journal of Gynecologic Oncology Vol.35 No.1
Objective: Lynch syndrome (LS) is a hereditar y cancer predisposition syndrome witha significantly increased risk of colorectal and endometrial cancers. Current standardpractice involves universal screening for LS in patients with newly diagnosed colorectal orendometrial cancer using a multi-step screening protocol (MSP). However, MSP may notalways accurately identif y LS cases. To address this limitation, we compared the diagnosticperformance of immediate germline sequencing (IGS) with MSP in a high-risk group. Methods: A total of 31 Taiwanese women with synchronous or metachronous endometrialand colorectal malignancies under went MSP which included immunohistochemical stainingof DNA mismatch repair (MMR) proteins, MLH1 promoter hypermethylation analysis, andgermline sequencing to identif y pathogenic variants. All patients who were excluded duringMSP received germline sequencing for MMR genes to simulate IGS for the detection of LS. Results: Our findings indicate that IGS surpassed MSP in terms of diagnostic yield (29.0% vs. 19.4%, respectively) and sensitivity (90% vs. 60%, respectively). Specifically, IGS successfullyidentified nine LS cases, which is 50% more than the number detected through MSP. Additionally, germline methylation analysis revealed one more LS case with constitutionalMLH1 promoter hypermethylation, bringing the total LS cases to ten (32.3%). Intriguingly,we obser ved no significant differences in clinical characteristics or overall sur vival betweenpatients with and without LS in our cohort. Conclusion: Our study suggests that IGS may potentially offer a more effective approachcompared to MSP in identif ying LS among high-risk patients. This advantage is evident whenpatients have been pre-selected utilizing specific clinical criteria.
Ji-Yeon Park,Angel Yang,Jong-Hyouk Park,A. M. Abd El-Aty,Jae-Ho Oh,Jung-Ah Do,Kisung Kwon,Ki-Hoon Shim,최옥자,심재한 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Separation of pesticides and other chemical contaminants from fatty food matrices prior to subsequent steps in the analytical process remains a challenging issue, and much effort has been invested to further enhance this method. The aim of the present study was to develop a simple multi-residue method involving a quick, easy, cheap, effective, rugged, and safe (QuEChERS)extraction method for the identification and quantification of 41pesticide residues in cooked fatty food matrices, including heated soybean oil, roasted sesame, and boiled soybean using gas chromatography-micro/electron capture detector (GC-μECD). The analytes were subsequently confirmed via GC-mass spectrometry (MS). The responses of analytes were linear with excellent correlation coefficients (r 2) ranging from 0.993 to 1.000 (calculated from absolute peak areas). For the majority of the tested pesticides, the mean recoveries ranged from 68.5 and 121.4% with relative standard deviations ranging from 0.4 to 18.7%. Instrument limits of detection and quantification ranged from 0.004 to 0.30 μg/kg and 0.0125 to 1.00 μg/kg, respectively. The developed method presented in this study was applied successfully to determine pesticide residue levels in cooked fatty food matrices. None of the samples contained detectable amounts of pesticide residues.
Park, Ji-Yeon,Yang, Angel,Park, Jong-Hyouk,Abd El-Aty, A.M.,Oh, Jae-Ho,Do, Jung-Ah,Kwon, Kisung,Shim, Ki-Hoon,Choi, Ok-Ja,Shim, Jae-Han The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Separation of pesticides and other chemical contaminants from fatty food matrices prior to subsequent steps in the analytical process remains a challenging issue, and much effort has been invested to further enhance this method. The aim of the present study was to develop a simple multi-residue method involving a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method for the identification and quantification of 41 pesticide residues in cooked fatty food matrices, including heated soybean oil, roasted sesame, and boiled soybean using gas chromatography-micro/electron capture detector (GC-${\mu}ECD$). The analytes were subsequently confirmed via GC-mass spectrometry (MS). The responses of analytes were linear with excellent correlation coefficients ($r^2$) ranging from 0.993 to 1.000 (calculated from absolute peak areas). For the majority of the tested pesticides, the mean recoveries ranged from 68.5 and 121.4% with relative standard deviations ranging from 0.4 to 18.7%. Instrument limits of detection and quantification ranged from 0.004 to $0.30{\mu}g/kg$ and 0.0125 to $1.00{\mu}g/kg$, respectively. The developed method presented in this study was applied successfully to determine pesticide residue levels in cooked fatty food matrices. None of the samples contained detectable amounts of pesticide residues.
Rekharsky, Mikhail V,Mori, Tadashi,Yang, Cheng,Ko, Young Ho,Selvapalam, N,Kim, Hyunuk,Sobransingh, David,Kaifer, Angel E,Liu, Simin,Isaacs, Lyle,Chen, Wei,Moghaddam, Sarvin,Gilson, Michael K,Kim, Kimo National Academy of Sciences 2007 Proceedings of the National Academy of Sciences Vol.104 No.52
<P>The molecular host cucurbit[7]uril forms an extremely stable inclusion complex with the dicationic ferrocene derivative bis(trimethylammoniomethyl)ferrocene in aqueous solution. The equilibrium association constant for this host-guest pair is 3 x 10(15) M(-1) (K(d) = 3 x 10(-16) M), equivalent to that exhibited by the avidin-biotin pair. Although purely synthetic systems with larger association constants have been reported, the present one is unique because it does not rely on polyvalency. Instead, it achieves its extreme affinity by overcoming the compensatory enthalpy-entropy relationship usually observed in supramolecular complexes. Its disproportionately low entropic cost is traced to extensive host desolvation and to the rigidity of both the host and the guest.</P>