Efficacy of Nile tilapia (Oreochromis niloticus) juveniles and spinosyns bioinsecticides against aquatic stages of Culex pipiens: An experimental study

Mohamed Ibrahim A.,Fathy Mohamed,Farghal Ahmed I.A.,Temerak Sobhy A.H.,Sayed Alaa El-Din H. 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The mosquito Culex pipiens is the most widely distributed dipteran species in all regions of Egypt and the principal vector of Wuchereria bancrofti and certain arboviruses in human beings. For controlling C. pipiens vector, biological tools (e.g., larvivorous fish and bioinsecticides) are more potent and safer options to the environment, human beings, and beneficial organisms than chemical pesticides. The efficiency of O. niloticus juveniles as predatory fish species and two bioinsecticides, spinosad 24% and spinetoram 12%, was investigated against the C. pipiens developmental stages in the laboratory. The first trial evaluated the predatory efficacy of small-sized O. niloticus (2.1–2.6 cm; 250–315 mg) and large-sized O. niloticus (2.5–3.2 cm; 250–315 mg) against the 3rd larvae and pupae of C. pipiens. This is the first report in Egypt confirming the predation potential of O. niloticus as efficient predatory fish against the immature C. pipiens. Large-sized O. niloticus predated a greater number of 3rd of C. pipiens larvae and pupae than the small-sized ones. Furthermore, the daily consumption of C. pipiens larvae by small- and large-sized O. niloticus was significantly higher than the pupae. The second trial assessed the toxicity efficacy of spinosad 24% and spinetoram 12% against C. pipiens larvae and pupae. The results confirmed that the tested bioinsecticides showed higher potency toward C. pipiens larvae than pupae after exposure for 24 h and 48 h. Spinosad was more toxic toward 3rd C. pipiens larvae (LC 50 = 0.013 and 0.003 mg/L) and pupae (LC 50 = 320.69 and 44.28 mg/L) than spinetoram after 24 and 48 h. Herein, O. niloticus juveniles (as promising native predatory fish) and spinosyns bioinsecticides were more effective against C. pipiens in the larval stage than in the pupal stage. In conclusion, Nile tilapia juveniles and biorational compounds, spinosad 24% and spinetoram 12%, might be considered as promising and favorable environmental biological agents for controlling C. pipiens in Egypt. However, further trials are needed to investigate the potential of these agents in the control of this mosquito vector under field conditions.

Electric, Thermoelectric and Magnetic Properties of Nickel(II) Imine Nanocomplexes

A. Elshafaie,Laila H. Abdel-Rahman,Ahmed M. Abu-Dief,Samar Kamel Hamdan,A. M. Ahmed,E. M. M. Ibrahim 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.7

Production of novel organic semiconductor nanomaterials is essential for enabling the development of personal, portable and flexible electronic modules. This work presents Ni(II)-Schiff base complexes with enhanced Seebeck coefficient and weak ferromagnetic ordering for thermoelectric and magnetic devices. Four Ni(II)-Schiff base complexes (namely [Ni(C12H10N3O4Br)] · 2H2O, [Ni(C16H11N3O4] · 2H2O, [Ni(C21H14N5O8Br)] and [Ni(C25H17N5O8)] · 1/2H2O) have been synthesized in nanosized dimensions. The electrical and thermoelectric properties have been studied, and comprehensive discussions have been presented to understand the electrical conduction mechanisms. The electrical conductivity measurements reveal that the conduction is due to the charge carriers hoping between the atomic sites of the same energy levels in the molecule as well as the transfer of the charge carriers between the neighboring complex molecules due to overlapping of their orbitals. The thermoelectric measurement confirms that the nanocomplexes (NCs) are non-degenerate P-type semiconductors with enhanced Seebeck coefficient values compared with those reported for other organic materials. The NCs exhibit antiferromagnetic to paramagnetic transitions with the increase of temperature and weak ferromagnetic ordering at 300 K.

Mineralogical studies and extraction of some valuable elements from sulfide deposits of Abu Gurdi area, South Eastern Desert, Egypt

Ibrahim A. Salem,Gaafar A. El Bahariya,Bothina T. El Dosuky,Eman F. Refaey,Ahmed H. Ibrahim,Amr B. ElDeeb 한국분석과학회 2024 분석과학 Vol.37 No.1

Abu Gurdi area is located in the South-eastern Desert of Egypt which considered as volcanic massivesulfide deposits (VMS). The present work aims at investigating the ore mineralogy of Abu Gurdi region inaddition to the effectiveness of the hydrometallurgical route for processing these ores using alkaline leachingfor the extraction of Zn, Cu, and Pb in the presence of hydrogen peroxide, has been investigated. The factorsaffecting the efficiency of the alkaline leaching of the used ore including the reagent composition, reagentconcentration, leaching temperature, leaching time, and Solid /Liquid ratio, have been investigated. It was notedthat the sulfide mineralization consists mainly of chalcopyrite, sphalerite, pyrite, galena and bornite. Gold isdetected as rare, disseminated crystals within the gangue minerals. Under supergene conditions, secondary copperminerals (covellite, malachite, chrysocolla and atacamite) were formed. The maximum dissolution efficienciesof Cu, Zn, and Pb at the optimum leaching conditions i.e., 250 g/L NaCO3 - NaHCO3 alkali concentration,for 3 hr., at 250℃, and 1/5 Solid/liquid (S/L) ratio, were 99.48 %, 96.70 % and 99.11 %, respectively. Anapparent activation energy for Zn, Cu and Pb dissolution were 21.599, 21.779 and 23.761 kJ.mol−1, respectively,which were between those of a typical diffusion-controlled process and a chemical reaction-controlled process. Hence, the diffusion of the solid product layer contributed more than the chemical reaction to control the rateof the leaching process. High pure Cu(OH)2, Pb(OH)2, and ZnCl2 were obtained from the finally obtained leachliquor at the optimum leaching conditions by precipitation at different pH. Finally, highly pure Au metal wasseparated from the mineralized massive sulfide via using adsorption method.

Assessment and Comparison of Commonly used Wireless Technologies from Mobile payment Systems Perspective

Ahmed H. Ali,Reham Abdellatif Abouhogail,Ibrahim F. Tarrad,Mohamed I. Youssef 보안공학연구지원센터 2014 International Journal of Software Engineering and Vol.8 No.2

Comparative Study of Itraconazole-Cyclodextrin Inclusion Complex and Its Commercial Product

Ibrahim A. Alsarra,Fars K. Alanazi,Sayed M. Ahmed,Ahmed A. Bosela,Suliman S. Alhamed,Hammam A. Mowafy,Steven H. Neau 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.7

Itraconazole (ITZ) solid complex using hydroxypropyl-β-cyclodextrin (ITZ-HP-β-CD) with 20% polyvinylpyrrolidone was prepared by a co-evaporation method. The complex improved antifungal activity against C. parapasilosis and C. albicans. The complex demonstrated good flow and compressibility characteristics. The complex was formulated as a capsule dosage form and drug release was evaluated. Capsules containing ITZ-HP-β-CD at a molar ratio of 1:3 with 20% polyvinylpyrrolidone have a faster dissolution rate than commercial capsules (Sporanox ®). About 88% of ITZ was released in less than 30 min and the initial dissolution rate exhibited a 3.5-fold increase compared to the commercial product. UV spectrophotometeric, HPLC, and antimicrobial methods were used to determine ITZ concentration in the release medium and the results obtained by these methods are reported. It was found that HPLC analysis is a suitable and reliable method for determination of the drug concentration with a coefficient of variation less than 10%. The intraday precision showed a coefficient of variation less than 3.96%, and that for interday was less than 4.99%. The HPLC method was more accurate and precise than the antimicrobial and UV-spectrophotometric methods for determination of ITZ concentration present in the release medium.

Activity and stability of purified amylase produced by streptomyces aureofaciens 77

Ibrahim, A.N.,Ahmed, F.H.,Ibrahim, M.M.K.,Arafa, M.A.I. The Pharmaceutical Society of Korea 1990 Archives of Pharmacal Research Vol.13 No.1

The effects of pH values, temperature and some elements on the amylolytic activity and stability of the purified S. aureofacienc 77 amylase were studied in this investigation. The purified enzyme showed its maximum activity at pH 6 within 8 min incubation at $40^{\circ}C$. None of the tested 6 metals showed on stimulatory effect on the enzymatic activity, $Fe^{+++}$, $Cu^{++}$ and $Hg^{++}$ at high dose inhibited the enzyme activity to great extent as compared with $Zn^{++}$, $Mn^{++}$ and $Fe^{++}$ whih gave less effect in this respect. The enzyme liquor was found to be thermolabile, since it lost completely its activity after 4 days incubation under room temperature and showed maximum activity during this period as a result of additions of $Ca^{++}$and NaCl, Gradual reduction was however recorded until activity reached 30% after 60 days of incubation.

Enhancement of chronic wound healing with maltodextrin/ascorbic acid gel: a clinical evaluation of distal limb wounds in horses

Ibrahim E. Helal,Hatim A. Al-Abbadi,Mohamed H. El-Daharawy,Mahmoud F. Ahmed 한국축산학회 2022 한국축산학회지 Vol.64 No.5

Delayed healing associated with distal limb wounds is highly challenging in equine clinicalpractice. This study aimed to evaluate healing rates between chronic non-granulatingwounds of horse distal limbs that were treated with maltodextrin/ascorbic acid gel alone orin combination with povidone-iodine 1% solution and those treated with povidone-iodine 1%only throughout the study period (35 days) in clinical settings. The study was conducted on18 adult horses (3–15 years old). Based on the treatment regimen utilized, the horses weredivided into three groups (n = 6), with each group having a similar mean wound area. Thepercentages of wound contraction, epithelialization, and overall wound healing were determinedweekly for each wound. By the end of the study, the total wound healing percentagewas significantly increased between the study groups (p < 0.05). The use of maltodextrin/ascorbic acid gel resulted in considerable wound contraction, rapid epithelialization, and complication-free wound healing. Based on the findings of this study, maltodextrin/ascorbic acidgel, independently or in combination with a 1% povidone-iodine solution, might be applied asa safe and effective wound healing promoting agent in horses with chronic non-granulatingwounds.

Precepitation and purification of amylase enzyme produced by streptomyces aureofaciens 77

Ibrahim, A.N.,Ahmed, F.H.,Ibrahim, M.M.K.,Arafa, M.A.I. The Pharmaceutical Society of Korea 1990 Archives of Pharmacal Research Vol.13 No.1

Precipitation and purification of amylase secreted by Streptomyces aureofaciens 77 in liquid inorganic salts-starch medium under the optimum conditions were carried out. Ammonium sulphate fractionation was used to precipitate amylase in cell free culture filtrate. (NH/sub 4/)/sub 2/ SO/sub 4/ at a concentration of 50-70% saturation gave the highest enzyme yield. The obtained precipitates were redissolved in phosphate buffer (pH 7.0) and subjected to dialysis. The dialyzed enzyme preparation was applied to DEAE-cellulose column chromatography which resulted in an increase of purification up to 59.48 fold. A further step of purification was done by applying the obtained purified sample to Sephadex-G200 column chromatography which resulted in ann increase of purification up to 73. 92 fold. The results clearly indicated that the isolated amylase from S. aureofaciens 77 was only on type.

Testing Whether a Survival Distribution is Better Mean Residual Life at Age t<SUB>0</SUB>

Ibrahim A. Alwasel,Ahmed H. El-Bassiouny 한국신뢰성학회 2006 International Journal of Reliability and Applicati Vol.7 No.1

Impacts of circulating cytokine levels and gene polymorphism predisposition on type 1 diabetes mellitus

Alghamdi Ahmed H.,El-Sherbini Sherif M.,Shatla Ibrahim M.,Mady Essam A.,El-Refaei Mohamed F. 대한소아내분비학회 2024 Annals of Pediatirc Endocrinology & Metabolism Vol.29 No.4

Purpose: A wide range of cytokines has been demonstrated to be involved in the etiology of type 1 diabetes mellitus (T1DM). Gene polymorphisms may potentially contribute to a hereditary predisposition toward circulating cytokine levels as (high, intermediate, or low) since they can affect cytokine production or function. The aim of this study was to investigate the roles of cytokine levels and the association of single-nucleotide polymorphisms (SNPs) within cytokine genes with T1DM in Saudi children.Methods: Totals of 91 well-characterized T1DM patients and 91 T1DM-free control subjects were enrolled in this study.Results: The levels of 3 circulating cytokines (transforming growth factor [TGF]-β1, interleukin [IL]-10, and IL-6) and 6 SNPs in 3 cytokine genes (TGF-β1 [rs1800470 and rs1800471], IL-10 [rs1800896, rs1800871, and rs1800872], and IL-6 [rs1800795]) that contribute to genetic susceptibility were measured by enzyme-linked immunosorbent assay and polymerase chain reaction with sequence-specific primers. Our fn dings show that TGF-β1 serum levels were signifcantly lower in the children with T1DM than in the control participants. The TGF-β1 genotypes with a high-production phenotype were signifcantly less frequent and those with a lowproduction phenotype were signifcantly more frequent in the children with T1DM compared to the control participants. respectively. Furthermore, the IL-6 genotype frequency with low level of IL-6 production were signifcantly increased in the T1DM group compared to the control group. Moreover, our data demonstrated no appreciable diferences in circulating serum level or genotype and phenotype of IL- 10 between the patients and controls.Conclusion: This kind of measurement, which considers the prediction of T1DM, may be useful in assessing the severity of T1DM and susceptibility to T1DM among Saudi children.