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HeLa세포배양을 이용한 콜레라균독소 및 항독소에 관한 연구
李鍾斗 대한감염학회 1975 감염 Vol.7 No.1
The present report concerns the toxin yield in the filtrates observed on the basis of cytopathic effect on HeLa cells in cultures, and immunogenic activities of cholera experimental vaccines consisting of both the toxic filtrates combined with the dead bacteria by mouse protection test. Cell free culture filtrates of Vibrio cholerae serotype Ogawa 41 were prepared by filtering through a 0.22U membrane filter peptone water which had 0.5∼5% peptone, pH6.5 or 7.8, and was cultivated at 29℃ for 17∼72 hours. Then, 17∼72 hour filtrates were inoculated on HeLa cells in monolayer cultures respectively and cytopathic effect was checked in 4 hours. Heated filtrates (56℃, 45 minutes) were also tested in this same way. In this way two kinds of toxins in the filtrates were found: heat labile toxin and heat stable toxin. These toxic filtrates were tested for mouse lethal activity, agglutinating, vibriocidal, and neutralizing antibody. Finally, experimental vaccines were prepared with the filtrates only and the filtrates plus dead cells in accordance with the conventional procedure for the preparation of cholera vaccine, and were tested for the protective activity against vibrio challenge by mouse protection test, compared with conventional vaccine. The results obtained are summarized as follows: 1) Heat labile toxin and heat stable toxin were found easily in cell-free culture filtrates of Vibrio cholerae on the basis of cytopathic effect on HeLa cells in culture. These toxins, which were also different in antigenicity, were neutralized specifically with their respective antiserum. Heat labile toxin was produced on culture at 29℃ in peptone water containing 0.5, or 1.0% peptone with nearly absence of heat stable toxin in 17 hour culture and the toxin appeared in the early stage of culture, the logarithmic phase. In contrast, heat stable toxin yield was greater on culture at 37℃ in peptone water containing above 3% peptone and the toxin appeared in the later stage of culture, the stationary phase. Activity of heat labile toxin was suppressed temporarily by the rabbit normal serum but heat stable toxin was not suppressed. 2) Toxicity of heat labile and stable toxic filtrates to mice increased in direct proportion to the peptone concentration in peptone water, incubation time and temperature, and heat stable toxic filtrate had a tendency to be more toxic than heat labile toxin. This tendency was similar to toxicity in vitro, based on cytopathic effects. 3) Agglutinins and vibriocidins were induced in rabbits at low and moderate level by both heat labile toxic filtrates and heat labile plus stable toxic filtrates respectively. The titers of agglutinins and vibriocidins were higher in heat labile toxic filtrates than heat stable toxic filtrates. 4) Experimental cholera vaccines consisting of dead cholera vibrio-containing toxic filtrates had more mouse protective activity than the conventional cholera vaccines but the cholera culture filtrates only, had similar protective activity to the cholera conventional vaccines diluted 100 times.
이종두 대한영상의학회 1989 대한영상의학회지 Vol.25 No.4
Since several reports were published about CT differentiation of peridiaphragmatic fluid collection using 4 useful signs-diaphragm displaced crus bare area and interface signs. Trans-verse CT scans of 20 patients with abnormal diaphragmatic position due to large intrathoracic or intraabdominal lesion were analysed on the basis of those sings. Difficulties were encountered with differentiation when laterally located lesions did not extend to as far medially as crus and when diaphragmatic stripe could not be distinguished from thickened pleura or adjacent wall of lesions. As a result limited cases can be adequately assessed by diaphragm or displaced crus sign. Furthermore bare area and interface signs seemed to be not useful at all. However relationship between caudal tip of lesions and thoracoabdominal wall was always constant in each thoracic or abdominal lesions. All of intrathoracic masses or empyemas were attached to thoracic wall displacing properitoneal and perirenal fat medially or inferiorly. By contraries all of intraabdominal mases were seperated from abdominal wall displacing proper-itoneal fat or peritoneum laterally. The key to accurate localization seemed to be identification of such relationship.
이종두 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.2
Rheumatoid arthritis(RA) is a chronic inflammatory disease of joints with proliferation of synovial epithelial tissue. Therapeutic approach of the RA consists of pharmacological and surgical interventions. Synovectomy is indicated in patients with progressive inflammatory signs and symptoms intractable to medical treatment including local intracavitary steroid injection. Recently, local injection of radionuclides which emit high energy beta rays are labeled with chemical compounds such as 90Y, 165Dy-ferric hydroxide macroaggregate and have been introduced as an alternative therapeutic modality to surgical synovectomy. Holmium-166 is one of beta emitter and Ho-166-chitosan complex was developed for radiation synovectomy. Preclinical trial is on-going at our hospital using Ho-166-chitosan. The procedure and methods of preclinical trial are discussed.