진동가진에 의한 엔진 배기매니폴드의 파손에 관한 연구

이경로,최복록 한국기계기술학회 2013 한국기계기술학회지 Vol.15 No.5

매미나방(Lymantria dispar) 유충의 혈림프 유리아미노산의 함량 변화

이경로,강정호,이해풍 한국곤충학회 1990 Entomological Research Vol.20 No.1

매미나방(Lymantria dispar) 유충의 발생에 따른 혈림프 유리아미노산을 HPLC를 사용하여 분석하였다. 유충의 혈림프에서 필수아미노산을 포함한 18종의 유리아미노산을 분리하였고, aspartic acid는 특이하게 3령 유충에서만 검출되었다. 전 발생단계에서 histidine이 최고함량을 나타냈고 다음으로 arginine과 lysine이 높게 나타났다. 영별 아미노산의 함량변화는 수컷 3령 유충에서 가장 높게 나타난 후 계속 감소하였으며, 암컷은 4령 유충에서 최고치를 보였고 감소하다가 7령 유충에서 다시 증가하였다. 한편, 총함량은 암컷이 수컷에서 보다 높게 나타났다. 일별 아미노산도 양적 변화를 보여 histidine이 전시기에 걸쳐 50% 이상이었고, arginine, lysine, serine등도 비교적 높았다. 일별 변화에서 총함량은 암 수 모두 5령 유충의 3일에서 가장 높게 나타났다. 수컷은 1일에서 최저치였으나 암컷은 2일과 5일에서 거의 비슷한 수준이었으며, 성별 비교에서는 암컷이 높아 영별 변화와 일치하였다. Free amino acids in the hemolymph during larva stages of the gypsy moth, Lymantria dispar were determined by HPLC. A total of 18 free amino acids including 10 essential amino acids were found but aspartic acid was present at 3rd instar larva only. Histidine was predominant amino acids, then arginine and lysine were shown to have high concentration at all stages. In male the concentration of total free amino acids decreased gradually from 3rd to 6th instar larva. At the 4th female larva, that of total free amino acids marked maximum. But it declined rapidly to 6th instar larva, and then followed by increase at 7th instar larval stage. During 5th instar larva development, free amino acids also showed changes in concentration 3 old day larva contained the highest content at both sexes. 1 old day male larva appeared the minimum level, whereas 2 old and 5 old day larvae marked low level at female.

Vanadate가 사람의 제대혈관의 장력에 미치는 효과

김민경,김기순,이경로 建國大學校基礎科學硏究所 1998 理學論集 Vol.23 No.-

혈관작용성 물질의 연구에 이상적이라고 알려진 제대혈관의 장력에 미치는 vanadate의 효과와 그 작용기작을 규명하기 위하여 실험하였다. Vanadate는 사람의 제대정맥과 동맥 모두에서 농도의존적인 수축을 일으켰으며, 모든 농도에서 제대정맥이 제대동맥보다 더 큰 수축반응을 나타내었고, Methylene blue ( )를 혈관 절편에 전처리하거나 화학적으로 제대혈관을 탈내피 시킨 후 (saponin 0.03㎎/ml, 45min) verapamil ( )으로 전처리한 경우 vanadate의 수축반응은 제대정맥과 동맥 모두에서 유의한 차이가 없었으며, Indomethacin ( )으로 혈관 단편을 전처리한 후에는 vanadate에 의한 수축반응은 제대정맥과 동맥 모두에서 현저히 억제 (각각 97.6±0.02%, 97.3±0.01%)되었고 제대혈관 절편을 OKY046 ( )으로 전처리시킨 후 vanadate,의 수축 효과를 관찰한 결과 전처리 전과 유의한 반응의 차이를 볼 수 없었다. 사람의 제대혈관에서 vanadate에 의해 유발된 수축은 내피세포 비의존성 수축으로 제대동맥보다 정맥에서 수축 반응이 더 컷으며 ,vanadate에 의한 수축은 TXA₂가 아닌 prostanoid계의 다른 수축 물질에 의해 일어남을 알 수 있었다. Vanadium is a trace element essential for the normal development and growth of the human and animals. Under physiological conditions, it is present in small concentrations in serum and tissues of the different animals. This anion is able to inhibit ATPase and ATPase of different tissues, including the cardiac and vascular smooth muscle. These enzymes play an important role in the regulation of the vascular tone. In fact, it was recently reported that vanadate induced contractions of the vascular and non vascular smooth muscles. On the other hand, human umbilical vessels are known to be ideal for the study of vasoactive substances because of poor innervation of autonomic nerves to these vessels. The aim of present study was to investigate effect of vanadate on the vascular tension of the isolated human umbilical vein (HUV) and artery (HUA) obtained from full-term deliveries after cesarean section and to elucidate the mechanism of these vanadate-induced vascular response. Vanadate ( ) induced concentration-dependent contraction of HUV and HUA preparations. At all concentrations, contracting effect of vanadate was greater in vein than artery. Pretreatment with methylene blue ( ), soluble guanylate cyclase inhibitor and verapamil ( ), L-type channel blocker, did not affect vanadate-induced contractions of umbilical vessels. After chemical denudation with saponin (0.03㎎/ml, 45min), the contractile response to vanadate was not changed. To test whether prostanoid is related to this contraction, vascular preparations were pretreated with indomethacin ( ), cyclooxygenase inhibitor. Indomethacin suppressed the contracile response by 97.6±0.02% in the umbilical vein and by 97.3±0.01% in the umbilical artery. In both types of preparations, vanadate-induced contractions were not altered by OKY046( ), TXA₂(thromboxan A₂) inhibitor. These results suggest that vanadate produces contracions both in the isolated HUV and HUA, and that the vanadate-induced contractions are mediated, at least in part, through prostanoids other than TXA₂.

밤바구미(Curculio dentipes Roelofs)의 유충.용의 혈단백질에 관한 연구

이경로,신병식 한국곤충학회 1988 Korean journal of entomology Vol.18 No.2

밤바구미의 각 시기에 따른 혈단백질 패턴과 함량을 SDS-polyacrylamide gel electrophoresis법과 spectrophotometer를 사용하여 분리측정하였다. 1. 혈단백질 함량은 월동전 유충에서 최고의 함량을 나타내었다. 월동중 감소하다가 월동후 다시 증가하였으며 종령유충기부터 계속 감소하여 응시기에 최저 함량을 나타냈다. 2. 혈단백질 패턴은 전시기에 걸쳐 최고 230,200 daltons에서 최저 15,900 daltons까지 모두 44개의 밴드가 나타났으며 가장 염색강도가 강한 밴드는 분자량 77,900 doltons으로 모든 시기에서 나타났다. 특히 용시기에서 단백질의 변화가 크게 나타났다 The pattrens and concentration of hemolymph protein in the chestnut weevil, Curculio dentipes (Roelofs) according to development stages were determined by SDS-polyacrylamide gel electrophoresis and spectrophotometric method. The protein contents were found to be the maximum at the nonoverwintering larva and decreased at the wintering larva and the overwintering larva and then reached minimum level at pupa stages. The denatured proteins were separated into 44 bands between the highest M.W. 230,200 daltons and lowest M.W. 15,900 daltons during all stages. The band of M.W. 77,900 daltons appeared during all stages and showed greatest color density. Particulary, at overwintering larva and pupal stages, large variation were observed in protein bands.

밤나무혹벌의 신생태종에 관한 연구(II)

이경로,이종진,신병식 한국곤충학회 1985 Korean journal of entomology Vol.15 No.2

재래종 및 저항성 밤나무에 기생하는 밤나무혹벌의 전 변태기 동안에 단백질의 패턴과 분자량을 SDS-polyacrylamide gel electrophoresis법을 이용하여 측정하였다. 1. 각 변태기중 유충기와 성충기 보다 용시기에서 더많은 단백질 밴드가 분리되었고, 같은 시기에 고분자단백 질의 밴드수가 증가되었다. 2. 전 변태단계에서 분자량 약 90,000~80,000과 50,000~40,000 부위가 2~3개의 주된 단백질 밴드로 분리되었다. 3. 재래종 밤나무에 기생하는 밤나무혹벌의 성충기에서는 다른 두 저항성 밤나무에 기생하는 밤나무혹벌 보다 더 많은 밴드가 분리되었으나, 밴드의 농도는 낮아서 다른 것과 현저한 차이가 나타났다. 4. 유충기의 경우, 재래 종은 축파와 단택에 기생하는 밤나무혹벌의 복합된 단백질 패턴을 보였다. 5. 밤나무혹벌간의 단백질 패턴의 차이는 기주식물에 따른 새로운 밤나무혹벌의 출현을 시사하며, 이 사실은 유전적 변이와 신생태종을 확인하는 기초자료가 된다. During metamorphosis, the protein patterns and major proteins molecular weight of chestnut gall wasp, Dryocosnus kuriphilus Yatsumatsu parasiting in susceptible chestnut and resistant chestnut were determinded by SDS-polyacrylamide gel electrophoresis. During pupal stage, more protein bands than those of the larval stages and adult stages were separated and the number of high molecular weight protein bands was increased at the same stage. Two or three major protein bands were distributed, whose molecular weights are about 80,000 to 90,000 and 40,000 to 50,000 at all developmental stages. A larger number of protein bands than those of two resistant strains were separated at the adult stage of susceptible strain and these protein bands showed remarkable differentation owing to lower color density. In case of larval stages, the susceptible strain showed the pattern of protein mixed with Cheuk Pa ana Dan Tak. It is conspicuous that changes of protein pattern of D. kuriphilus according to the host plants are caused by genetic variation and will he give the basal data in relation to the ecospecies.

솔잎혹파리의 변태에 따른 용기의 체액분석

이경로,이종진,이상석,고진복 한국곤충학회 1982 Korean journal of entomology Vol.12 No.2

The concetration of total protein and non protein nitrogen, lipid, trehalose, acid$.$alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and mineral salts during the pupal stage in the pine leaf gall midge, Thecodiptosis japonensis Uchida et Inouye, were measured by using micro-Kjeldahl method, Thin layer chromatographic method, Anthrone method, Reitman-Frankel method, Oser method and EEL dame photometric method respectively. Healthy specimens were chosen as samples of each developmental stages; the prepupa and the late pupa. The concentration of total protein was 88.139mg/g in the prepupa and was 120.719mg/g in the late pupal stage. Non protein nitrogen contents was 2.292mg/g in the prepupa add was 4.225mg/g in the late pupal stage. In the late pupal stage total protein and non protein nit-rogen contents was higher than those in the prepupal stage. Total lipid, phospholipid, menoacylgly cerol, free fatty acid, sterol and ester cholesterol were identified in the prepupa and the late pupal stage. The concentration of total lipid was 150.0mg/g in the prepupa and was 162.8mg/g in the late pupal stage. With the exception of total lipid, phospholipid, monoacylglycerol, free fatty acid, sterol and ester cholesterol contents were higher than in the late pupa those in the prepupal stage. Rf value of trehalose analyzed on thin layer chromatogram was 0.714 in both stages, and the concentration of trehalose was 3.640mg/g in the Prepupa and was 3.553mg/g in the late pupal stage. The activity of acid phosphatase was 0.272unit/mg in the prepupal and was 0.281unit/mg in the late pupal stage, and alkaline phosphatase was 0.413unit/mg in the prepupa and was 0.546unit/mg in the late pupal stage. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase showed the same patterns wi th acid . alkaline phosphatase. Mineral salts present were calcium, phosphorus, sodium and potassium, which were identified in both stages. All kinds of mineral salts contents in the late pupa were higher than those in the prepupal stage. In view of controlling pest, the patterns of the physiological activties indicate that control will be the most effective at the time of the worst physiological manifested at the prepupal stage. 솔잎혹파리 (Thecodiplosis japonensis Uchida et Inouye)의 변태기 중 전용과 후용의 액체 변화를 분석하고 그 활성을 측정코자 micro-Kjeldahl법, Thin layer chromatography법, Anthrone법, Reitman-Fra-nkel법, Oser법, EEL flame photometer법으로 측정분석하였다. 1)전용과 후용에서 총단백질과 비단백성질소, 지질, 탄수화물, phosphatase, GOT, GPT, 무기염류의 함량과 화성도가 측정되었다. 2) 총단백질과 비단백성질소 모두 후용에서 높은 함량의 변화를 나타냈다. 3) 분석된 지질은 총지질, 인지질, monoacylalycerol, 유리지방산, sterol, triacylglycerol, ester cholesterol이였으며 triacylglycerol을 제외하고 후용에서 각 지질의 함량이 전용에서 보다 높았다. 4) trehalose의 함량은 큰 차이는 없었으나 전용에서 다소 높았다. 5) 효소 활성도의 변화는 acid.alkaline phosphatase, GOT, GPT 모두 후용에서 높았으며, GOT는 GPT에 비해 매우 높은 활성을 보였다. 6) 무기염류는 칼슘, 인, 나트륨, 칼륨이 분석되었으며, 칼륨이 가장 높은 함량을 나타냈다. 7) 전용과 후용의 생리적 활성을 비교하면 전용기가 생리적 기능하강기로 나타나 이 시기가 생물학적 방제의 최적기로 이용될 수 있을 것이다.

한출변화과정(汗出變化過程)을 통(通)한 태음인(太陰人) 만성해수(慢性咳嗽) 치험례(治驗例)

이경로,이상기,송정모,Lee, Kyung-Ro,Lee, Sang-Gi,Song, Jung-Mo 사상체질의학회 2004 사상체질의학회지 Vol.16 No.3

1. Objectives In this study, it is experienced that the process of sweating is important in chronic cough of Taeumin. In this case report, the patient has been simproved throuth reaction of sweating improvement. In "Dongyi Soose Bowon", The process of sweating change is described in detail. "Hamkeylhae" is similar to chronic cough in "Dongyi Soose Bowon", it is cough that is hard to spit. The chronic cough of Taeumin is related to the pathology of Liver and Lung, sweating condition is also related to condition of Lung in Sasang constitutional medicine. So the study for the process of sweating is needed. 2. Methods One patient diagnosed as chronic cough was practiced with Sasang constitutional medicine. And We observed sweating change process during treatment. 3. Results As sweating condition improved, chronic cough also decreased, but not improved, not decreased. 4. Conclusions So treating of Taeumin's chronic cough is closely connected with sweating change process.

한국산 밤나무흑벌의 변태에 따른 지방산에 관한 연구

이경로 한국통합생물학회 1965 동물학회지 Vol.8 No.2

Fatty acid components of the Chestnut gall wasp (Dryocosmus kuriphilus YASUMATSU) were analyzed by the method of gas chromatogrphy at various developmental stages. 1. The fatty acids identified at each stage are as follows : Larva stage : acetic acid , benzoic acid and capric acid. Prepupa stage : acetic acid, benzoic acid and capric acid. Pupa stage : acetic acid, benzoic acid and capric acid. 2. Through the development of the wasp, there were large amount of acetic acid.

한국산 화랑곡 나방의 유리 아미노산에 관한 연구

이경로 한국통합생물학회 1964 동물학회지 Vol.7 No.1

The free amino acid content of Indian meal moth (Plodia interpunctella HUBNER) was analysed at various developmental stages by means of paper chromatography. 1) The free amino acids : present are alanine , arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, methionine, proline, serine, threonine, tyrosine and valine. 2) Proline was detectable only in the acid-hydrolyzed Indian meal moth. 3) Arginine was clearly detected only in the larva stage. 4) Tyrosine methionine and valine were increased in the pupa stage. 5) Serine, glycine and tyrosine were present in high concentration in all stages.

함나무혹벌 신생태종에 관한 연구(I)

이경로,이종진 한국곤충학회 1982 Korean journal of entomology Vol.12 No.1

In order to clarify the comparision of biological characteristics between susceptible and resistant hosts of the chestnut gall wasp, Dryocomus kuriphilus Yasumatsu, morphological and physiological investigation that is the concentration of free amino acid, protein nitrogen, non protein nitrogen, trehalose, lipids, mineral salts and respiratory activity were measured, respectively. The results are summarized as follows; Morphological investigation of the egg, the ovipositor, the antennae, the wing and the leg were measured, but can not found significant differences between the two varieties of the chestnut gall wasp. In susceptible varieties, amino acids were detected in a total of 16 but amino acids of resistant varieties were detected in a total of 13 The amino acids identified at each varieties are alanine, aspartic acid, cysteine, glutamic acid, glycine, histidine, leucine, methionine, serine, threonine and valine. In general, protein nitrogen, non protein nitrogen and trehalose content of resistant were more than susceptible. Also, total lipid' phospholipid, monoacylglycerol, free fatty acid, triacylglycerol and ester cholesterol were identified at each stage in both varieties, and changes of concetration through the course of the physiological cycle form a U-shaped curve during metamorphosis. The respiratory activities had no significant differences between susceptible and resistant varieties. The mineral salts presented were sodium, calcium, potassium and phosphorus, which were identified at each stage in both varieties. The mineral salts concentration of susceptible was by far lower than that resistant of the chestnut gall wasp. In conclusion, of the two ecospecies of the chestnut gall wasp which damaged susceptible and resistant varieties, morphological differences were insignificant, however, physiological differences brought out the metabolic pattern that resistant was more than susceptible. 감수성품종과 저항성품종 밤나무를 가해하는 밤나무혹벌의 생태학적 특성을 비교하기 위하여 형태학적 조사와 변태에 따른 아미노산, 단백성질소, 비단백성질소, 탄수화물, 지질, 무기염류, 호흡능의 활성도에 대한 생리학적 조사를 한 결과는 다음과 같다. 1) 형태학적 조사를 위하여 란ㆍ산란관 촉각, 시맥, 각의 형태및 크기를 관찰하였으나 두 품종간에 유의한 차이를 찾을 수 없었다. 2) 아미노산은 감수성품종의 밤나무혹벌에서 16종, 저항성품종, 밤나무혹벌에서 13종이 검출되었고 alanine, aspartic acid, serine, valine은 모두 높은 농도를 나타냈다 3) 단백성질소ㆍ비단백성질소와 trehalose 모두 저항성품종의 밤나무혹벌이 높은 함량을 나타냈다. 4) 지질은 총지질, 인지질, monoacylglycerol, 유리지방산, triacylglycerol, ester cholesterol 이 분리되었으며 변태에 따른 생리적 U자형 곡선을 나타냈다. 지질의 함량은 저항성품종의 밤나무혹벌이 감수성품종의 그것보다 높았다. 5) 호흡능의 활성도는 감수성품종과 저항성품종의 밤나무혹벌에서 일정한 경향성을 보이지 않았다. 6) 무기염류의 함량변화는 변태기별로는 유충기와 전용기에 높았고, 저항성품종의 밤나무혹벌이 다소 높은 함량을 나타냈다.