활천을 여는 글 : 여교역자회전국연합회 신임회장 - 이군자 전도사 ( mini interview )

이군자 기독교대한성결교회 활천사 2002 활천 Vol.587 No.-

韓國 小年非行에 對한 小考 : 豫防과 善導외 諸問題點

李君子 이화여자대학교 사회사업학과 1967 사회사업 Vol.- No.2

日本紀行 : 이웃나라 일본의 인상

이군자 경기대학 관광학회 1967 觀光學報 Vol.1 No.-

Mouse 신장으로부터 Ornithine Decarboxylase의 정제

이군자,조영동 한국생화학회 1992 BMB Reports Vol.25 No.5

자성 mouse의 피하층에 testosterone을 투여하여 ornithine decarboxylase의 활성을 증가시킨 후, 신장으로부터 ornithine decarboxylase를 황산암모늄 분별침전, DEAE-sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5'-phosphate agarose affinity chromatography를 이용하여 분리 정제하였다. 이 효소의 분자량은 SDS-polyacrylamide gel electrophoresis, Sephacryl S-200 gel chromatography와 ornithine decarboxylase의 효소활성 억제제인 14C-DFMO 을 효소와 결합시켜 얻은 autoradiography를 이용하여 52,000 dalton인 동일한 subunit으로 구성된 dimer임을 알 수 있었다. 정제된 효소의 ornithine에 대한 K_(m) 값과 V_(max) 값은 각각 167㎛과 5 nmol CO_(2)/h로 나타났고, pyridoxal-5-phosphate에 대한 K_(m) 값과 V_(max) 값은 각각 0.142㎛과 2.4nmol CO_(2)/h로 나타났다. 효소의 최적 pH 는 7.0, 최적 온도는 37℃이었으며, 정제된 ornithine decarboxylase는 polyamines(putrescine, spermidine, spermine)에 의해 효소 활성이 영향을 받지 않았다. 본 실험에서는 ornithine decarboxylase의 분리과정 중에서 ornithine decarboxylase 활성을 안정화시키는 물질을 발견하였다. Ornithine decarbosylase (ODC) was purified to homogenity from testosterone treated mice kidney by ammonium sulfate fractionation, DEAE-Sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5-phosphate-agarose affinity chromatography. The specific activity of ODC was 14,000 U/㎎, and purification fold was 1,180. The enzyme has a Mr. of about 110,000 dalton and is a dimer of subunit Mr. 52,000. The K_(m), for L-ornithine was 167㎛ and for pyridoxamine phosphate, 142 nM. ODC has an optimum pH of 7.0 and optimum temperature of 37℃. The purified enzyme was not inhibited by polyamine (putrescine, spermidine and spermine). During purification process, ODC stabilizing material was found.

염화 Cadmium 이 흰쥐 간장에 미치는 영향에 관한 전자현미경적 연구

강신덕,정호삼,이규식,이군자 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

실험동물은 본 실험실에서 순종교배에 의해 육성시킨 Wistar계 흰쥐를 사용하였으며, 순종교배에 의해 출생 제 7일의 신생 흰쥐 (체중 10g내외) 출생 제 40일된 흰쥐 (체중 120g내외)를 사용하였다. 신생 흰쥐와 성숙 흰쥐에 각각 cadmium chloride를 체중Kg당 3.75mg되게 생리적 식염수에 용해하여 0.2ml을 복강내에 투여한 후 3시간, 6시간, 12시간, 24시간 및 36시간 후에 각각 희생하고 개복하여 간장을 절취하였다. 간세포의 미세구조를 관찰하기 위해 조직은 4% glutaraldehyde에 전고정하였으며 10% osmium tetroxide로 후고정 후 초박절편을 제작하여 uranyl acetate 와 lead citrate로 이중 염색을 시행하였으며 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. cadmium chloride 투여 신생 흰쥐의 간실질세포에서 골지체는 3시간군, 6시간군및 36시간군에서 소조가 위축되었다. 사립체는 3시간군에서 팽대되었고, 24시간군에서는 사립체롱이 소실되었으며 심한 팽대가 관찰되었고, 36시간군에서는 불규칙한 형태를 보이나 사립체롱은 정상적으로 관찰되었다. 조면내형질세망은 3시간 군에서 소조의 팽대와 일부 낭상화가 관찰되었으며 6시간군에서는 낭상화와 막부착 ribosome 과립의 탈락을 볼 수 있었고 24시간군과 36시간군에서는 대부분의 소조가 낭상화되었다. 2. cadmium chloride 투여 성숙 흰쥐의 간실세포에서 골지체는 3시간군에서 소조가 다소 팽대되었고 24시간군에서는 소조의 수가 감소되었다. 사립체는 6시간군에서 팽대되었고, 12시간군에서 불규칙한 형태를 보였으나 36시간군에는 대조군과 유사한 구조로 관찰되었다. 조면내형질세망은 6시간군, 12시간군 및 24시간군에서 소조의 팽대, 낭상화 및 막부착 ribosome과립이 탈락을 관찰할 수 있었고 36시간군에서는 대조군과 유사한 구조로 관찰되었다. Phagosome이 3시간군과 6시간군에서 다수 관찰되었고 12시간군, 24시간군 및 36시간군에서는 자가용해소체와 잔여소체를 볼 수 있었으며 특히 36시간군에서 증가되었다. 이상과 같은 결과로 미루어 cadmium chloride를 신생 흰쥐와 성숙 흰쥐에 투여하였을 때 미세구조상 신생 흰쥐 간세포의 미세구조는 투여후 시간이 지남에 따라 정상구조와 유사한 상태로 회복되는 것으로 사료된다. It is known that cadmium exposure to human results in very harmful adn potenteally toxic to liver and kideny. Therefore, the authors were undertaken to pursue the acute effects of cadmium chloride on the ultrastructures of rat hepatocytes, The 7 days old newborn and adult Wistar rats(40 days old) were used as experimental animals. Both new born and adult rats were injected intraperitoneally with 3.75 mg/kg cadmium chloride, 0.2ml(diluted with 0.9% NaCl solution). The sections of the experimethtal animals were made and observed by electron microscope. The results obtained were as follows. 1. In the hepatocytes of cadmium chloride treated newborn rats, cisternae of Golgi complexes are atrophied in the 3hr, 6hr. and 36hr. groups. The mitochondria are hypertrophied in 3hr. group. Cristae of mitochondria are reduced and swollen in 6hr. group. The cisternae of rough endoplasmic reticulum are dilatated and partly sacculated in 3hr. group and the associated ribosome granules are datached. In the 24hr. and 36hr. group, the most cisternae are sacculated. 2. In the hepatocyte of cadmium chloride treated adult rats, the cisternae of Golgi complexes are slightly dilatated and reduced in 24hr. group. Mitochondria are hypertrophied in 6hr. group and seen irregular shapes in 12hr. group but in 36hr. group, most mitochondria are intact as well as that of control rat groups. Rough endoplasmic reticulum showes dilatation, sacculation and detachment of associated ribosome granules from cisternase in 6hr. 12hr. and 24hr. group. But in 36hr. group. the fine structures of rough endoplasnmic reticulum are intact. The secondary lysosomes, phagosomes, are observed in 3hr. and 6hr. groups and numerous autophagic vacuoles and lipofucin granules are seen in 12hr. and 24hr. gruop. The large lipofucin granules are increased in 36hr. group. Consequently it is suggested that cadmium chloried made several toxic effects to hepatocytes of newborn and adult rat, the damage of hepatocyte increased in adult rats and newborn rat.

최루가스(o-Chlorobenzylidene Malononitrile)가 흰쥐의 각막과 결막에 미치는 영향

이군자 서울보건대학 1995 서울보건대학 부설 한국보건과학연구소 논문집 Vol.2 No.1

Two groups of rats were exposed to the o-chlorobenzylidine malononitrile(CS) being used in the control of riots, and sacrificed to examine histological and histochemical changes of the cornea and conjunctiva. 1. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the cornea. Epithelial and endothelial Na,^(+) K^(+) -ATPase activity were reduced, wing cells of the epithelium were transformed to the squamous shape and mitotic activity occurred actively in the basal layer. 2. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, Na^(+), K^(+)-ATPase, Mg^(2+)-ATPase and alkaline phosphatase activities of the corneal epithelium and endothelium were reduced. And there was epithelial shrinkage, irregular basement membrane and irregularly arranged collagen fibers were also observed in the stroma. 3. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the conjunctiva. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated. 4. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, irreversible changes were observed. Desquamation with oedema and shrinkage of the epithelial cells, and loss of some basement membrane were observed. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated significantly. As a result when eyes are temporally exposed to the CS gas reversible degenerative changes, desquamation with oedema of the epithelium and infiltration are produced. And when eyes are repeatedly exposed to the CS gas histopathologic changes such as desquamation with oedema of the epithelium, infiltration, and reduced mucin secreting activity are occurred.

태생기 및 신생기 흰쥐 간장에서의 Putrescine 대사에 관한 연구

이군자 서울保健大學 1992 論文集 Vol.12 No.1

신생아 집중치료실에서 퇴원한 환아 부모에게 전화상담한 내용과 간호중재에 대한 조사연구

이군자 京畿專門大學 2000 京畿專門大學 論文集 Vol.- No.28

The purpose of this study was to identity the current status of nursing intervention by telephone. A head nurse of the intensive care unit wrote down the content of interview with parents of newborn discharged from hospital from 7 a. m. to 3 p. m. during the period of February through December in 1999. The content of 150 telephone calls except incomplete record among 460 calls were analyzed into frequency of general characteristics, content and nursing intervention by 11 categories. The findings of this study were as follows; 1. The proportion of males to females, male patients(50.0%) female patients(50.0%) by the ratio of 1:1 and the most common age was 1-2 weeks. 2. The most common admission period was within 1 week. 3. The most common diagnosis during admission period was jaundice, premature. 4. The most frequent contents were physical symptoms and vital sign, a jaundice and diarrhea among physical symptoms, fever and respiratory difficulty among vital sign. 5. Nursing intervention through telephone was identified with order to confirmation, direction, education, inducement. 6. The most frequent nursing intervention by telephone was good condition in confirmation, diarrhea in direction, feeding amount in education, follow up care in inducement. These results suggest that telephone call intervention program should be established as afield of extended pediatric nursing role in health care delivery system for the newborn.

운동프로그램이 중년비만여성의 체구성, 체력 및 지질대사에 미치는 효과

이군자 한국간호과학회 2005 Journal of Korean Academy of Nursing Vol.35 No.8

Purpose: This study was to examine the effects of an exercise program for middle-aged obese women. Method: The exercise program combined folk dance and resistance training. The subjects group consisted of 85 middle-aged obese women between 40 and 60 years of age. Three 8 week sessions consisted of a 55-80% maximum heart rate (MHR) exercise for 60-90 minutes a day and 3 times a week from March to November, 2004. Data was collected through a pre- and post-exercise test before and after each session. Data was collected with Inbody, dynamometer and blood. This data was analyzed by descriptive statistics, and a paired t-test with an SPSS/PC(10.0 version) program. Results: There were significant positive changes in body weight, body fat mass, body mass index, percent body fat, muscle strength, muscle endurance, flexibility, and balance quality, but no significant positive changes in skeletal mass, fat free mass, percent abdominal fat(waist-hip ratio), visceral fat area, agility, total cholesterol, high density lipoprotein cholesterol, or triglycerides. Conclusion: This study showed that an exercise program has partially positive effects for middle-aged obese women. The results of this study show that exercise at community health centers should continue for middle-aged obese women's health.

운동프로그램이 성인여성의 체력, 심폐기능 및 생활만족도에 미치는 효과

이군자 지역사회간호학회 2005 지역사회간호학회지 Vol.16 No.2

The Effects of an Exercise Program on Physical Fitness, Cardiopulmonary for Adult Women Purpose : This study was to examine the effects of an exercise program on physicalfitness, cardiopulmonary function and lifesatisfaction for adult women. Method : Theexercise program combined dance and resistancetraining. The subject group consisted of 114 women aged between 33 and 60. Three 8-week sessions consisted of 55-80% HR max for 60-90 minutes a day and 3 times a week from March to November, 2004. Data were collected through pre- and post-exercise tests before and after each session. Data were collected with dynamometer, sphygmomamometer, spirometer and structured questionnaires. Data were analyzed employing descriptive statistics and paired t-test with SPSS/PC(10.0version) program. Results: There were significantly positive changes in muscle strength, flexibility, balance quality, forced vital capacity and life satisfaction, but no significantly positive change in agility and blood pressure. Conclusion : This study showed that an exercise program has partially positive effects for adult women. The result of this study suggest that there should be programs of continuous exercise at community health centers for adult women's health.