Escherichia coli 의 Succinate Dehydrogenase 에 미치는 인삼 Saponin 의 영향

조영동,김길룡,김일 ( Young Dong Cho,Kil Lyong Kim,Yil Kim ) 생화학분자생물학회 1980 BMB Reports Vol.13 No.1

In this experiment, it was attempted to observe the possible effect of Korean ginseng saponin on the growth and one of the most important enzyme in TCA cycle, succinate dehydrogenase (E.C. 1. 3.99. 1. ), of E. coli w3110 to make a contribution to revealing partially the physiological action of ginseng saponin. The following results were obtained: 1) When the concentrations of ginseng saponin in the assay mixture were between 1×10^(-1) % and 6×10^(-1)%, E. coli soluble SDH was highly activated; the maximum activity of E. coli soluble SDH was shown in the presence of 4×10^(-1) ginseng saponin and its activity was inhibited below 1×10^(-2)%. The ㎞ value for succinate of E. coli soluble SDH was 5.05×10^(-3)M. 2) At 4×10^(-1)% saponin solution, E. coli membrane bound SDH was inhibited significantly but concentrations above 2×10^(-1)% saponin solution were innocuous for the enzyme. The km value for succinate of E, coli membrane bound SDH was 2.58×10^(-3)M. 3) When the E. coli were grown in the media containing 1×10^(-1)% ginseng saponin, there was increase in lag phase of E. coli. However, the growth curve of E. coli was similar to that of control when the concentrations of ginseng saponin was between 1×10^(-2)% and 1×10^(-4)%. 4) When the E. coli were grown in the media containing 1×10^(-1)% ginseng saponin, the activity of soluble SDH was increased 26% in compare with that of control whereas the activity of soluble SDH seemed to be degreased in E. coli grown in the media containing 1×10^(-2) and 1×10^(-3)%. 5) After preincubation at various temperature, E. coli soluble SDH was highly activated when the concentration of ginseng saponin was 4×10^(-1)%. The optimum temperature of E. coli soluble SDH was at 2℃ under condition described. 6) After storage with and without ginseng saponins at 2℃ for 9,21, and 27 hours, the activities of soluble SDH were almost similar.

권두언

조영동 생화학분자생물학회 2001 생화학분자생물학회 소식 Vol.21 No.1

N/A

CNS Culture 와 이의 이용

조영동 생화학분자생물학회 1976 BMB Reports Vol.5 No.2

터널굴착에서 다단식 발파기에 의한 전단면 발파의 적용성 연구

조영동,이상은,임한욱 대한화약발파공학회 1995 화약발파 Vol.13 No.1

Many methods and techniques to reduce ground vibrations are well known. Some of them are to adopt electric milisecond detonators with a sequential blasting machine or an initiating system with an adequate number of delay intervals. The types of electric detonators munufactured in Korea include instantaneous, decisecond and milisecond delays byt numbers of delay intervals are only limite from No.1 to No.20 respectively. It is not sufficient to control accurately milisecond time with these detonators in tunnel excavation. Sequential fire time refers to adding an external time delay to a detonators norminal firing time to obtain sequential initiation and it is determined by sequential timer setting. To reduce the vibration level, sequential blasting machine with decisecond detonatore was adopted. A total of 134 blasting was recorded at various sites. Blast-to-structure distances ranged from 20.3 to 42.0 meter, where charge weight varied from 0.25 to 0.75 kg per delay. The results can be summarized as follow : 1. The effects of sequential blasting machine on the vibration level are discussed. The vibration level by S.B.M. are decreased approximately 14.38~18.05 to compare to level of conventional blasting and cycle time per round can be saved. 2. The empirical equations of particle velocity were obtained in S,B.M. and conventional blastin. $V=K(D/W^{1/3})-n$. where the values for n and k are estimated to be 1.665 to 1.710 and 93.59 to 137 respectively. 3. The growth of cracks due to vibrations are found but the level fall to within allowable value.

A Study on the Effect of Ginseng Saponin Fraction on Cell Wall(II) : Cell Wall of Bacillus subtilis

조영동,김남걸,김충원,Cho, Young-Dong,Kim, Nam-Geol,Kim, Choong-Won 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.4

본 실험에서는 인삼 주 성분 중의 하나인 saponin 분획이 Bacillus subtilis의 성장, U-$^{14}C$-glucose의 uptake 속도, lysis 및 세포벽 등에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. 0.3% 인삼 saponin 분획이 첨가된 배지에서 자란 B. subtilis의 성장속도는 control 배지에서 자라난 것 보다 배양 시작한지 6시간 후 부터 빨라졌다. 2. 0.3% 인삼 saponin 분획이 첨가된 배지에서 자란 B. subtilis는 control 배지에서 자란 것보다 U-$^{14}C$-glucose의 uptake 속도가 빨라졌다. 3. 0.3% 인삼 saponin 분획이 첨가된 배지에서 자란 B. subtilis의 lysozyme에 의한 lysis는 control 배지에서 자란 것 보다 빠르다. 4. 광학 및 전자 현미경의 관찰에 의하면 0.3% 인삼 saponin 분획이 첨가된 배지에서 자란 B. subtilis와 control 배지에서 자란 B. subtllis 사이에는 세포벽의 변화가 있었다. In this experiment, observations were made on the effects of ginseng saponin, one of the major components of Korean ginseng (Panax ginseng, C.A.Meyer) root, on the growth rate of Bacillus subtilis, the uptake rate of the U-$^{14}C$-glucose, the lysis, and cell wall, The followings were obtained. (1). The growth rate of B. subtilis grown on medium containing 0.3 % ginseng saponin was faster than that of B. subtilis grown on medium without it after 6 hr from the beginning of culture. (2). The uptake rate of the U-$^{14}C$-glucose by B. subtilis grown on medium containing 0.3 % ginseng saponin was faster than that of B. subtilis grown on medium without it. (3). The lysis of B. subtilis grown on medium containing 0.3 % ginseng saponin fraction was faster than that of B. subtilis grown on medium without it. (4). Cell wall of B. subtilis grown on medium containing 0.3 % saponin fraction was different from that of B. subtilis grown on medium without it.

The Effect of Ginseng Saponin on Secretion of Bile Acids and Absorption of Lipids in Intestine of The Rat

조영동,김충원,김일,Cho, Young-Dong,Kim, Choong-Won,Kim, Il 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.3

본 실험에서는 한국산 인삼의 주요 활성 성분의 하나인 인삼 saponin이 장내의 지방 흡수에 필요한 답즙산 분비 속도와 농도 및 작은 창자에서 지방흡수에 미치는 영향을 in vovo 에서 관찰한 바 다음과 같은 결과를 얻었다. 1. Saponin은 본 실험의 조건하에서 담즙의 분비량에는 영향을 주지 않으나 담즙내의 cholate 농도에는 2~4.5배의 증가를 보여준 반면, 혈액내의 gastrin양에는 영향을 주지 못했다. 2. 또한 cannulation방법에 의해 담즙 및 췌장액이 작은 창자로 유출되지 않는 경우 saponin이 시간에 따른 작은 창자에서의 지방흡수 속도를 약 2배 촉진시킨다. 3. 담즙 및 췌장액이 작은 창자내로 유출되는 경우 시간에 따른 장내 지방흡수를 촉진시킨다. In this experiment, it was attempted to observe the effect of saponin, one of the major compounds of Korean Ginseng, on secretion of bile acids and absorption of lipids in the small intestine of the rat, in vivo, and the following results were obtained. 1) Under the experimental condition described, it was observed that the ginseng saponin did not affect the volume of bile juice secreted from the liver but increased 2 to 4.5 times the concentration of cholate contained in the bile juice and the gastrin level in the blood. 2) In the case of cannulation method of obstructing the entrance of bile juice and pancreatic juice into the small intestine, the ginseng saponin stimulated about 2 times the rate of absorption of lipids in the small intestine of the rat. 3) Without cannulation method, the ginseng saponin also stimulated the absorption of lipids in the small intestine of the rat.

Effects of the Ginseng Saponin Fraction on Growth of Micoroorganisms ( 2 )

조영동,김남걸 생화학분자생물학회 1985 BMB Reports Vol.11 No.4

인삼 Alkaloid 분획이 탈수소효소에 미치는 영향

조영동,한정호,김옥희 ( Young Dong Cho,Jung Ho Han,Ok Hee Kim ) 생화학분자생물학회 1974 BMB Reports Vol.7 No.1

The enzymic activities of three dehydrogenases obtained from chicken were measured in vitro after alkaloid fraction was administered. Alkaloid fraction activates dehydrogenases in the following order: succinate dehydrogenase, serum lactic dehydrogenase and glutamic dehydrogenase, whereas muscle lactate dehydrogenase is deactivated. The presence of alkaloid was tentatively identified by using spectroscopic methods, nitrogen analysis and some color reactions.

담배 알카로이드 분획이 닭 간장 Succinate Dehydrogenase 에 미치는 영향 ( 1 )

조영동,안문환 ( Yong Dong Cho,Moon Hwhan Ahn ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.3

The effect of tobacco alkaloid fraction on SDH was studied. At low concentration of the alkaloid fraction, succinate dehydrogenase was activated whereas it was inhibited at high concentration of the alkaloid fraction. Inhibition of SDH by malonate was partially relieved by the alkaloid fraction.

The Effect of Ginseng Saponins on Escherichia coli Succinate Dehydrogenase

조영동,김길룡,김일,Cho, Young-Dong,Kim, Kil-Lyong,Kim, Yil 생화학분자생물학회 1980 한국생화학회지 Vol.13 No.1

본 실험에서는 한국산 인삼의 주요성분인 Saponin (Panax ginseng C.A. Meyer)이 장내미생물인 E. coli 성장과 TCA 회로에 관여하는 효소중 가장 활성이 크고 중요한 Succinate dehydrogenase활성에 미치는 영향을 관찰함으로써 인삼 Saponin의 생리적 작용의 일부를 밝히고자 실험을 하여 다음과 같은 결과를 얻었다. 1. Succinate에 대한 E. coli Soluble SDH의 Km은 $5.05{\times}10^{-3}M$이며 assay mixture에서의 인삼 Saponin 농도가 $1{\times}10^{-1}%{\sim}6{\times}10^{-1}%$에서 Soluble SDH의 활성이 크게 증가되었으며, $4{\times}10^{-1}%$에서 가장 큰 활성증가를 나타냈으나 $1{\times}10^{-2}%$ 이하에서는 활성에 별 영향이 없었다. 2. Succinate에 대한 E. coli membrane-bound SDH의 Km 값은 $2.58{\times}10^{-3}M$이며 인삼 Saponin의 농도가 $1{\times}1O^{-5}{\sim}2{\times}10^{-1}%$에서는 활성에 큰 영향을 주지 못했고, $4{\times}10^{-1}%$ 이하에서는 커다란 활성강소 현상을 나타냈다. 3. $1{\times}10^{-1}%$의 인삼 Saponin이 포함된 medium에서 배양한 E. Coli의 경우, 대조군에 비해 lag phase가 약 2배 긴 반면 $1{\times}10^{-3}%$의 경우는 약간의 성장촉진 현상을 나타냈다. 4. $1{\times}10^{-2}{\sim}1{\times}10^{-3}%$의 인삼 Saponin이 포함된 medium에서 배양한 E. coli의 Soluble SDH 활성은 대조군에 비해 낮은 현상을 보였으나, $1{\times}10^{-1}%$의 경우 약 26% 높은 현상을 나타냈다. 5. E. coli Soluble SDH의 척적 preincubation 온도는 $20^{\circ}C$이며, 가장 활성이 좋은 때의 인삼 Saponin의 농도는 $4{\times}10^{-1}%$이었다. 6. Soluble SDH 활성감소도는 $1{\times}10^{-1}%$, $1{\times}10^{-2}%$의 Saponin을 첨가한 경우나 첨가하지 않은 경우냐 거의 비슷하였다. In this experiment, it was attempted to observe the possible effect of Korean ginseng saponin on the growth and one of the most important enzyme in TCA cycle. succinate dehydrogenase (E.C. 1.3.99.1.), of E. coli w3110 to make a contribution to revealing partially the physiological action of ginseng saponin. The following results were obtained: 1) When the concentrations of ginseng saponin in the assay mixture were between $1{\times}10^{-1}%$ and $6{\times}10^{-1}%$, E. coli soluble SDH was highly activated; the maximum activity of E. coli soluble SDH was shown in the presence of $4{\times}10^{-1}%$ ginseng saponin and its activity was inhibited below $1{\times}10^{-2}%$. The km value for succinate of E. coli soluble SDH was $5.05{\times}10^{-3}M$. 2) At $4{\times}10^{-1}%$ saponin solution. E. coli membrane bound SDH was inhibited significantly but concentrations above $2{\times}10^{-1}%$ saponin solution were innocuous for the enzyme. The km value for succinate of E. coli membrane bound SDH was $2.58{\times}10^{-3}M$. 3) When the E. coli were grown in the media containing $1{\times}10^{-1}%$ ginseng saponin, there was increase in lag phase of E. coli. However, the growth curve of E. coli was similar to that of control when the concentrations of ginseng saponin was between $1{\times}10^{-2}%$ and $1{\times}10^{-4}%$. 4) When the E. coli were grown in the media containing $1{\times}10^{-1}%$ ginseng saponin, the activity of soluble SDH was increased 26% in compare with that of control whereas the activity of soluble SDH seemed to be decreased in E. coli grown in the media containing $1{\times}10^{-2}%$ and $1{\times}10^{-3}%$. 5) After preincubation at various temperature. E. coli soluble SDH was highly activated when the concentration of ginseng saponin was $4{\times}10^{-1}%$. The optimum temperature of E. coli soluble SDH was at $2^{\circ}C$ under condition described. 6) After storage with and without ginseng saponins at $2^{\circ}C$ or 9, 21, and 27 hours, the activities of soluble SDH were almost similar.