고냉지농업연구실편 : 소맥에 있어서 이면교잡에 의한 Nitrata Reductase Activity 의 조합능력과 유전인자작용분석

홍병희(B . H . Hong),송융남(Y . N . Song) 강원대학교 농업과학연구소 1989 강원 농업생명환경연구 Vol.3 No.-

고령지농업연구실 ; 완두에 있어서 성숙기와 주요형질들간의 경로계수분석과 유전상관

홍병희(B . H . Hong),송융남(Y . N . Song),조현묵(H . M . Cho) 강원대학교 농업생명과학연구원 1984 강원 농업생명환경연구 Vol.1 No.-

N/A N/A

A Path-coefficient analysis and genetic correlation between important traits in pea (pisum sativum L.)

B. H. Hong(洪丙憙),Y. N. Song(宋隆男),H. M. Cho(趙賢黙) 한국육종학회 1984 한국육종학회지 Vol.16 No.1

Twenty cultivars of pea(Pisum sativum L.) were grown in the field of Kangwon National University. They were evaluated for maturity and other characters. The relationships between values measured were analysed by the method of path coefficient. Genotypic correlation coefficients were larger than phenotypic ones. The first flowering node number, flowering time and plant height were highly correlated with maturing time. Genotypic correlation between maturing date and the first flower node number was highly significant, 0.5893 which was made up largely of a direct effect of 1.5844. A highly significant correlation was likewise obtained for the comparison between maturing date and flowering time, being influenced mainly by the indirect effect via first flower node number. The association between earliness and plant height was moderately significant.

차세대 리소그래피 빛샘 발생을 위한 플라스마 집속장치의 아르곤 아크 플라스마의 방출 스펙트럼 진단

홍영준,문민욱,이수범,오필용,송기백,홍병희,서윤호,이원주,신희명,최은하,Hong, Y.J.,Moon, M.W.,Lee, S.B.,Oh, P.Y.,Song, K.B.,Hong, B.H.,Seo, Y.H.,Yi, W.J.,Shin, H.M.,Choi, E.H. 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.6

차세대 리소그래피 기술인 극자외선(EUV : Extreme Ultraviolet) 빛샘 연구의 기초단계로써, 동축타입의 전극구조가 설치된 다이오드 챔버를 통해 Ar 플라스마를 생성하였으며, 방출 분광기술(emission spectroscopy)를 이용하여 방출된 가시광선 영역의 빛을 조사하였다. 장치의 입력 전압을 0.5kV씩 변화를 주어 $2\sim3.5kV$까지 인가를 했으며 이극챔버의 최적 압력인 330mTorr 일 때 각 전압에 따른 방출 분광선 데이터를 얻었다. 이때 Ar I과 Ar II 방출선을 관측하였으며 국소적인 열적평형 (LTE ; Local Thermodynamic Equilibrium) 상태의 가정 하에 볼츠만 도표(Boltzmann plot)와 사하(Saha) 방정식을 이용해 Ar I 및 Ar II의 전자온도와 이온 밀도를 각각 계산하였다. 각 입력전압에 대해 이온밀도는 Ar I과 Ar II에서 각각 $\sim10^{15}/cc$ 및 $\sim10^{13}/cc$의 값으로 계산되었다. We have generated the argon plasma in the diode chamber based on the established coaxial electrode type and investigated the emitted visible light for emission spectroscopy. We applied various voltages $2\sim3.5kV$ to the device by 0.5kV, and obtained the emission spectrum data for the focused plasma in the diode chamber on the argon pressure of 330 mTorr. The Ar I and Ar II emission line are observed. The electron temperature and ion density have been measured by the Boltzmann plot and Saha equation from assumption of local thermodynamic equilibrium (LTE) The Ar I and Ar II ion densities have been calculated to be $\sim10^{15}/cc\;and\;~10^{13}/cc$, respectively, from Saha equation.

황색종 잎담배의 엽위별 엽형질 유전에 관한 연구

신승구,홍병희,류익상,Sin, Seung-Gu,Hong, B.H.,Ryu, I.S. 한국연초학회 1992 한국연초학회지 Vol.14 No.2

The magnitude of additive effects generally parralled the magnitude of difference between parental means and appeared to be more independent from non - allelic interaction than dominance effects, whereas the magnitude of dominance effects were inflated by non-allelic interaction. Additive effects were significant for all characteristics observed and those of leaf width and leaf shape index of lugs, cutter and leaf and leaf width of tips were higher than dominance effects. Non-allelic gene interaction was expressed in leaf length of lugs, cutter and tips and heterosis of these characters were lower than the other characters.

맥류의 종속간 교잡에 관한 연구 제1보 교배방법 및 교배촉진물질의 처리가 교잡종자 생산에 미치는 영향

김봉연,홍병희,조장환,제상율,Kim, B.Y.,Hong, B.H.,Cho, C.H.,Je, S.Y. 한국작물학회 1982 한국작물학회지 Vol.27 No.2

The trials were carried out for improvement of early maturing wheat varieties, desease resistant and good quality through transfering good characters of barley to the wheat, during 1980-'81. For these purpose, Chinese Spring, a wheat variety was used as male and Manker 16 a barley variety, as female parents. EACA($\varepsilon$-Amino Caprolic Acid) known as an inhibitor of blood coagulation was injected to all barley from 14 days until heading with 1$m\ell$ per culm at the level of 500ppM. $GA_3$ solution which accelerates pollen activity was also used with 75ppM concentration after pollination for two days including check to determine the single effect of EACA. Two different methods of the cross were used. One was bud-pollination, another was post-pollination. The result indicated that the post-pollination was better than bud-pollination in terms of pollination rate, even though formation rate of normal embryo was seemingly adversed. The new plant derived from barley-wheat cross was similar in phenotype to the wheat.

Nicotiana tabacum과 N. rustica 체세포 잡종식물의 육성

최상주,이승철,홍병희,Choe, Sang-Ju,Lee, S. C.,Hong, B. H. 한국연초학회 1993 한국연초학회지 Vol.15 No.2

Mesophyll protoplasts derived from young leaves of Nicotiana rustica and N. tabacum cv Burley 21 were fused with the aid of polyethylene glycol(PEG). Cytological examination of protoplasts after PEG treatment revealed 12.8 % heterokaryocytes. After 7 weeks culture, the hybrid calli showing greenish white with a compact appearance were selected in contrast to parental type calli tinged with white or green color. The somatic hybrid plants were verified by morphological, biochemical and cyclological analysis. A heterosis effect for plant vigor and height was observed but the shape of leaves and flower characteristics were intermediate between N. tabacum and N. rutstica. The isozyme banding patterns for peroxidase of somatic hybrid lines were compared with the parent species. A number of isozyme bands derived from both parental species were found in the hybrids. Somatic hybrid plants have been successfully backcrossed to the parental N. tabacum particularly with somatic hybrid plants as female parents. These hybrid plants yielded small seeds, only few which were germinable.

韓國에 있어서 小麥 半矮性遺傳子의 起湲, 傳播 및 利用에 關한 硏究

C.H. CHO(曺章煥),B.H. HONG(洪丙憙),M.W. PARK(朴文雄),J.W. SHIM(沈載昱),B.K. KIM(金鳳九) 한국육종학회 1980 한국육종학회지 Vol.12 No.1

Origin, dessemination and utilization of semi-dwarf genes of wheat were studied. Begining of wheat cultivation in Korea was about 3000 years ago and varietal name was given around in 1400 A.D. Original population carrying semi-dwarf genes was found to be Korean native Anzunbaengimil or Nanjangmil which was introduced to Jappan in the period of 1904~1905 and presumably renamed as Daruma. Genetic resemblance of these two populations was discussed. Number of pure-line selections were made from this population and Norin 10 was released in Japan by utilizing this semi-dwarf genes. Korea also released Suweon 92 and Seuseun 27 using same gene sources. Contribution of this semi-dwarf genes to the increase of world wheat production was reviewed. Semi-dwarfing genes of wheat which extended a great contribution for increasing wheat production in the world were investigated on their origin, dissemination, and utilization and the results were summarized as followings. 1. Begining of wheat cultivation in Korea was postulated to be about 3000 years ago considering the history of wheat cultivation of China and Japan. 2. Wheat varietal name was recorded as early as in 1482. However, Nongsajiksul published in 1400 has a brief description about wheat (cultural) methods. Therefore, it is not difficult to presume that varietal name was available to that time. 3. Original population where the semi-dwarf plants were selected were Anzunbaengimil or Nanjangmil which were distributed all where in Korea in the period of 1500~1941. This population had great variation in plant height, presumably a mixture with individuals carrying Rht₁, Rht₂ or Rht₁ Rht₂. 4. Dissemination of Anzunbaengimil to Japan was possiblly made during the period of Imjin Japanese Invasion to Korea, or by the Japanese Agronomists who were dispatched to Korea for investigation of agriculture and land productivity from 1901 to 1905. 5. Anzunbaengimil(Crippled wheat) may be named as Daruma(crippled wheat) after introduction to Japan or renamed as Daruma after pure-line selections were made. Considering such traits as growth habit, glume color, plant, height grain color and plant type it was concluded that Daruma is orginated from Auzunbaengimil population. 6. In Japan, semi-dwarf gene from Daruma population have been utilized extensively and released Norm 10 which became an important world wide breeding material. Seuseun 27 and Suweon 92, the Korean semi-dwarfs, were also improved using same dwarf gene source as Norin 10. 7. Dwarf genes from Daruma population, have contributed for improving several early maturing and high yielding wheat varieties in Korea such as Suweon 85, Chokwang, Suweon 215, 216, 219 and 220. 8. Norin l0/Brfvor 14 was the dwarf gene source for improving Cains and Nugains at Washington State, USA and CIMMYT semi-dwarfs. Coullee, a hard white winter wheat, was improved using Suweon 92, and Seuseun 27 was utilized for improving Tam Wheat and Holley wheats at Texas A&M, USA.

日長條件 따른 小麥의 稈長變異 및 遺傳에 關한 硏究

C.H. CHO(曺章煥),B.H. HONG(洪丙憙),J.H. NAM(南重鉉),B.H. CHANG(張炳浩) 한국육종학회 1980 한국육종학회지 Vol.12 No.2

To investigate the genetic and ecological variation of culm length of wheat the different daylength has been applied to major wheat varieties planted at greenhouse in Suweon. Standard errors of culm length were larger at 12 and 8hrs day length than that of 24hrs. Variations of culm length due to different photoperiod were more remarkable in medium and short culms than that of long one. Number of genes controlling culm length of variety Robin #2 was more or less 2 genes. This study has been conducted to investigate the genetic and ecological variation of wheat calm length measured from those plants grown at greenhouse. The results are as following. 1. The calm length of the winter wheat was highest under 12hrs daylength, while that of the spring wheat was highest under 8hrs daylength. Standard errors of culm length was larger under 12 and 8hrs daylength than that of 24hrs daylength condition. 2. The culm length was shortest under the 24hrs daylength compare to under 12 or 8hrs while it was highest under 12hrs. Short and medium culm length varieties were photo insensitive but those of long culm length were photo sensitive. 3. There are many types of varietal differences for the culm length under the daylength conditions. Highest culm length group under 12 and 8hrs contition was classified. The semi-dwarf varieties that did not change their plant heights under 8 and 12hrs conditions were Hira, Yecora Resel, Sava, Sturdy, Saric 70, Sekidorisai 66 etc. 4. Varietal difference of culm elongation was noticed between 8hrs and 12hrs daylength condition. On the basis of the evidences obtained from this experiment, it may be concluded that the genetic studies related to culm length should be carried out under the most favorable conditions for calm elongation. 5. Correlation coefficients estimated between culm length and heading or maturity were negative but insignificant, but their correlations were highly significant between photo treatments. 6. Calm length of F₁ was intermediate to the range of F₂ population which seems to be distributed normally. In this regard the gene involved with calm length seemingly inherit additively. Statistical analysis for the involved genes for calm length suggested 2 genes for the semidwarf variety Robin #2 was more or less 2 genes.

맥류의 세대촉진방법에 관한 연구 IV. 수원지역에서 소맥 1년 2기작 세대촉진재배

성병열,조장환,박문웅,홍병희,안완식,남중현,Seong, B.Y.,Cho, C.H.,Park, M.W.,Hong, B.H.,Ahn, W.S.,Nam, J.H. 한국작물학회 1980 한국작물학회지 Vol.25 No.4

To establish a generation acceleration technique, two crops a year at field condition of Suweon, 10 varieties different in their spring growth habit were tested with 6 different seeding time after harvesting. These materials were harvested on June 10, 19, 79 and tested for their seed production ability at varions seeding time from July I I to August 15 with a week interval. An immatured seed germination technique and green vernalization methods were applied in cycling generations and the results obtained were summarized as follows. 1. In summer growing, seedlings establishment after transplanting was better in earlier transplanting. 2. Heading time was remarkably enhanced by earlier transplanting. Considering the results of two years early or mid of July was the suitable time to plant the second summer crop. 3. Those varieties of spring growth habit expressed little variations in plant height among the varieties. In 1978 which is referred as warm year produced plant height as tall as 8-16cm and poor crop but produced good crop with 25-65cm plant height in 1979. 4. No definit tendency in the length of spike was. observed among the cultivars but longer spike was found in winter wheat compared to the spring. 5. Number of spikes per plant was ranged from 1 to 3 regardless the transplanting time in 1979. However, more spikes per plant were produced in early or mid of July transplanting and those varieties of higher growth habit than V did not produces any spikes. 6. Higher number of grains per spike was found at earlier transplanted varieties. Therefore, it is concluded that those materials of I-IV growth habit with mid or early July transplanting would be suitable in practical sense considering their ability of seed production. 7. Two-year results indicated that wheat crop can not tolerate the temperature level higher than average 32$^{\circ}C$ C at Suweon. In this regard, the cultivation schedule was established assuming average temperature condition like the year of 1979 which was possible to grow wheats.