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        ITS 염기서열 분석 및 CAPS를 이용한 조이시아 속(Zoysia) 들잔디와 갯잔디의 구별

        홍민지(Min-Ji Hong),양대화(Dae-Hwa Yang),정옥철(Ok-Cheol Jeong),김양지(Yang-Ji Kim),박미영(Mi-Young Park),강홍규(Hong-Gyu Kang),선현진(Hyeon-Jin Sun),권용익(Yong-Ik Kwon),박신영(Shin-Young Park),양바오로(Paul Yang),송필순(Pill-Soon So 한국원예학회 2017 원예과학기술지 Vol.35 No.3

        Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica , Z. sinica , Agrostis stolonifera , and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica , nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica , 22 Z. sinica , and 4 Z. japonica /Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica . Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.

      • 세포소기관 형광발현 형질전환체와 바이러스 단백질 위치화 고찰

        홍민지 ( Min-ji Hong ),주호종 ( Ho-jong Ju ) 전북대학교 농업과학기술연구소 2016 농업생명과학연구 Vol.47 No.1

        Cells are surrounded by a cell membrane as a basic unit constituting the organism is found in all organisms, including plants. Subcellular organelles specialized structural unit performing a specific function within cell. Studies on the location in the protein of the organelles in a cell biological point of view to understand the function of the protein may provide valuable important information about the role of the protein on a series of processes occurring within the cell. Although there are a number of different ways, the method of using GFP (green fluorescent protein) has been used most commonly to check out the degree and location of gene expression and other cell biological purpose. Many researches have been developed subcellular organelle markers on model plants. Arabidopsis thaliana has been known the most useful model plant in many fields of plant science and transgenic Arabidopsis expressing fluorescent proteins at various subcellular organelles were developed to study the protein localization. However, Nicotiana benthamiana has been used as the best model plant to facilitate cell biological studies for most plant viruses. Therefore, if N. benthamiana transgenic plants expressing fluorescent proteins at subcellular organelles are exist, that will be useful in plant virus localization study.

      • KCI등재후보

        핵심정보기술의 보호를 위한 관련법규의 형사법적 검토

        홍민지(Hong Min Ji) 인하대학교 법학연구소 2008 法學硏究 Vol.11 No.3

        글로벌 지식기반경제로의 이행이 본격화됨에 따라 정보기술의 확보와 함께 원천기술의 창출능력이 국가의 경쟁력을 좌우하고 있다. 현재 우리나라는 조선ㆍ반도체ㆍ자동차 등 주력산업에서 지속적인 첨단제품의 개발과 함께 제조 및 생산기술력을 바탕으로 Global leadership을 확보하고 있으며, 특히 우리나라가 개발한 ‘와이브로’가 지난해 3세대 이동통신 세계표준으로 선정되는 등 우리의 기술력이 국제적으로 인정을 받고 있다. 그러나 이러한 국가핵심기술에 있어서 꾸준한 개발과 국가적 지원이 물론 필요하겠지만, 이보다 더 중요한 것은 이렇게 개발한 기술력을 강하게 보호하는 것이라 하겠다. 따라서 본 연구에서는 최근 들어 유출범죄에 가장 많이 사용되는 수단인 인터넷 등 정보통신망과 관련한 ‘정보통신망 이용촉진 및 정보보호 등에 관한 법률’(이하 “정보통신망법”이라고 약칭한다)과 유출범죄가 발생한 경우 이에 대한 수사를 위해 요구되는 절차 등을 규정한 ‘통신비밀보호법’의 관련조항에 관한 형사법적인 문제점 검토 및 개선방안을 연구하도록 하겠다. This study is focused on the relevant legal regulation’s problems and improvements from point of view of criminal laws for protecting of the core information technology. Therefore, this paper examined realistic problems concerning laws on protecting the outflow of IT and high-tech technology and search for a complete and effective regulation for the whole IT industry. So, the purpose of the paper was to prevent infringement of trade secrets and industrial technology, etc that damages and losses were worsened day by day and to protect effectively. The study suggested reasonable standards that laws and regulations of prevention of high-tech technology could help solve current problems. The study was focused on investigating the actual conditions of the technology outflow to start legislation. Recently, for example Hacking(computer intrusion) and E-mail, wiretrap a telephone is beginning to represent an increasing share of total high-tech technology outflowing methods use. The core-information technology belonged to properties of enterprises as well as the government. An advanced criminal investigation system should be made to detect infringement, and reasonable and harmonized criminal law should be applied. The core-information Technology could not undo losses and damages at leakage so that preliminary prevention of infringement was required rather than post criminal regulations.

      • KCI등재
      • KCI등재

        첨단기술의 유출방지를 위한 관련법규의 형사법적 문제점과 개선방안에 관한 연구

        홍민지 ( Min-ji Hong ) 한국지식재산연구원 2008 지식재산연구 Vol.3 No.1

        This research studies problems of certain laws and regulations in a criminal law aspect, which are established to effectively protect high technology and prevent infringement where advanced information and technology of domestic corporations are leaked to competing countries, causing great losses to related corporations and the national economy. The study examines criminal-law-related problems of a special law, the 'law for leak prevention and protection of industrial technology', which was legislated in 2006 and became effective in April 2007, and provides plans to improve the overall laws and regulations related to high technology leak prevention. Of all special laws, many questions have been raised regarding the most recently established and enforced 'law for leak prevention and protection of industrial technology' even before the law was established. The questions include provisions defining the concept of subjects of protection, provisions on forms of leaks and infringement acts, and criminal punishment clauses. Although numerous examinations have been made regarding such problems, certain parts are still remaining in the law which may raise issues in a criminal law perspective. Therefore, improvement plans are proposed regarding the laws for prevention of unfair competition and protection of trade secrets where the criminal-law-related issues have been much discussed, whereas the discussion is centered around the industrial technology leak prevention law in the part of 'examination of criminal-law-related problems' of the same law. The proposed improvement plans for the current laws and regulations related to preventing high technology leaks are discussed in two separate parts - provisions on criminal penalties and provisions on criminal actions. Improvement plans for the provisions on criminal actions are discussed in two parts of legal procedures and investigation practice. In the conclusion, the overall research discussed is summarized and proposals are provided for prevention of illegal leaks and infringement of high technology and effective protection of high technology.

      • KCI등재
      • KCI우수등재SCOPUS
      • KCI등재

        액상환원법을 이용하여 합성된 은 나노입자의 적외선 흡수 및 반사 특성

        홍민지 ( Min Ji Hong ),박민지 ( Min Ji Park ),김종화 ( Jong Hwa Kim ),( Ashish A. Rokade ),진영읍 ( Young Eup Jin ),이근대 ( Gun-dae Lee ),박성수 ( Seong Soo Park ) 한국공업화학회 2017 공업화학 Vol.28 No.5

        액상환원법을 통하여 출발 물질인 질산은 용액으로부터 환원 용매인 DMF와 환원제 및 계면활성제 역할을 하는 PVP를 이용하여 여러 합성 조건에서 균일하면서 적당한 크기의 은 나노판상체를 합성하고자 하였다. 합성 시료 및 필름시편들의 특성들은 SEM, TEM, UV-Vis-NIR 분광법, PSA 및 XRD를 사용하여 비교 조사하였다. 질산은 용액과 DMF에 26 wt%의 PVP가 첨가된 반응물을 70 ℃에서 72 h 동안 반응하여 합성한 시료에는 약 100~200 nm 크기의 삼각형판상체들로 존재하였고, 약 1,000 nm에서 최대 흡수 피크를 나타내므로 근적외선을 가장 잘 반사시키는 시료로 예측하였다. 필름용 코팅액에서 은 나노판상체의 함유량이 증가하거나 이의 크기가 증가되면, 필름 시편의 투과율은 떨어지고 반사율은 높아지는 경향을 나타내었다. Uniform and optimum sized silver nanoplates were synthesized through the liquid phase reduction method by using silver nitrate solution as a starting chemical, dimethylformmide (DMF) as a reducing solvent, and polyvinylpyrrolidone (PVP) as reducing and surfactant agents. Synthesized and also film samples were characterized by using SEM, TEM, UV-Vis-NIR spectroscopy, particle size analyzer (PSA), and XRD. Triangle nanoplates with the size of 100~200 nm were found from the sample synthesized at 70 ℃ for 72 h using silver nitrate, DMF and 26 wt% PVP. The sample could reflect near-infrared light because it showed the maximum absorbing peak at about 1,000 nm. When the content or particle size of silver nanoplates increased in coating solutions, the transmittance decreased and the reflectance increased in film samples.

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