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부루세라 RB51의 ELISA 진단법 개발 II. Brucella abortus RB51균의 8kDa 항원 정제 및 ELISA 진단법 개발
허문,조동희,정병열,조성근,정석찬,김옥경,Her, Moon,Cho, Dong-hee,Jung, Byeong-yeal,Cho, Seong-kun,Jung, Suk-chan,Kim, Ok-kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.1
A procedure for extraction and purification of 8 kDa antigen of Brucella abortus RB51 was developed. Bacteria heat inactivated at $60^{\circ}C$, 30 min was extracted by 1% sarcosine and followed by fluid pressure liquid gel filtration chromatography of 2 series, Superose 12 HR 10/30 and Sephacryl S-100. There was produced $71.46{\mu}g/g$(wet) of 8 kDa antigen, and it resisted 1% trypsin, solved 1% triton X-100 higher than distilled water and inactivated 0.1% proteinase K. These results show that 8 kDa antigen may be a lipoprotein existed cell surface of B. abortus RB51. Also, we developed ELISA using purified 8 kDa surface antigen of Brucella abortus RB51 strain, its specificity and sensitivity was 95.0%, 98.6%, respectively. As compared with dot-blot assay using whole cell and ELISA using 8 kDa antigen, its correlation was 93.5%.
부루세라 RB51의 ELISA 진단법개발 I. Westeren blot에 의한 Brucella abortus RB51균의 항원 분석
허문,조동희,정병열,조성근,정석찬,김옥경,Her, Moon,Cho, Dong-hee,Jung, Byeong-yeal,Cho, Seong-kun,Jung, Suk-chan,Kim, Ok-kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.1
As compared with reaction of antibody for sonicated antigen of Brucella abortus strain RB51 and 1119-3 by Western blot analysis, Brucella field positive sera was detected strong reaction at 40~80 kDa LPS of strain 1119-3, but detected very weak reaction at strain RB51 partly. Otherwise, as we analyzed major immunogen of RB51 by antisera bled periodically during 6 months after RB51 vaccination. we detected strong immunological reaction at 17, 18 and 8 kDa antigen of RB51. Especially, reaction of 8 kDa antigen by Western blot coincided with reaction of dot-blot assay in RB51-antibody detection method. We also compared with reaction of field sera by STAT(standard tube agglutination test), dot-blot assay and Western blot (reaction of 8 kDa antigen of strain RB51). 16 sera of 4~5 months after RB51 vaccination are all negative by STAT, and 12 field brucellosis positive serum are all positive, and also 12 of 16 sera vaccinated RB51 are positive by dot-blot assay and reaction of 8kDa antigen by Western blot. but 1 of 15 Brucellosis negative sera reacted nonspecifically dot-blot assay.
허문도,Heo, Mun-Do 대한양돈협회 1988 養豚 Vol.10 No.6
신생자돈의 환경온도는 생시부터 7일까지 30-50$^{\circ}C$, 습도는 60-80%가 좋으며, 모돈은 12-23$^{\circ}C$ 범위이다. 한편, 방서대책의 원리로 돈사내의 기류를 촉진시켜 주고 복사열을 방지해야 한다
허문만,이인재,윤현보,Hur, Moon-Man,Lee, In-Jae,Yoon, Hyun-Bo 한국전자파학회 2006 한국전자파학회논문지 Vol.17 No.10
임의의 필드 분포를 갖는 단일 페치 안테나를 평면 배열하고 각 소자에 공급되는 급전 신호의 크기와 위상을 유전자 알고리즘으로 최적화하여, 여러 방향의 지향성과 널을 갖는 다중 빔 복사 패턴의 합성 방법을 제안하였다. 제안된 방법의 유효성을 확인하기 위해서, $2{\times}2$ 평면 배열 안테나를 제작하고 원거리 복사 패턴의 계산 결과와 측정 결과를 비교하여 동일한 특성을 확인하였으며, 다중 빔 복사 패턴의 합성은 $8{\times}8$ 배열 안테나 시스템을 이용하여, 시뮬레이션을 통해서 각 소자의 급전 신호의 크기와 위상을 최적화하였다. In this paper, the synthesis method of multibeam radiation pattern with multi-directional beams and nulls is proposed. Genetic algorithm is applied for the optimization of amplitude and phase of the feeding signal into each element that is structured in planar array of single patch antenna with an arbitrary field distribution. To verify the validity of the proposed method, $2{\times}2$ planar array antenna is used for the measurement and the measured far field radiation pattern is compared with the simulation result. As a result, the synthesis of multibeam radiation pattern is implemented by the optimization of amplitude and phase of the feeding signal into each element of $8{\times}8$ planar array antenna system.