http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
토양과 곤충 사체로부터 곤충병원성 선충의 분리 및 동정
한상미(Sang Mi Han),한명세(Myung Sae Han) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3
Nematodes were isolated using silkwom trap through the investigation of 100 soil samples from various biotopes in Korea. The 30 nematode strains from soil and dead insects by the pathogenicity aganinst silkworms (Bombyx mori mori) and insect pests of Calliphora vomitoria, Pseufaletia separata, Palomena angulosa, and Melolontha incana. Mortailty of the silkworm larvae and pupae were as high as 100% by nematode infection, those of insect of pests were varied from 20 to 100%. The 30 strains of entomopathogenic nematodes were classified into five groups of Rhabditidae, Diplogatroidae, Heterorhabitidae, Steinernematidae, and Tylenchida by morphological criteria. The genetic relationships among the 30 nematode strains were analyzed by various RAPD bands with twenty primers. The 30 nematode strains were classified into six major subgroups on the basis of the genetic similarity coefficient of 0.853. The grouping by RAPD was agree with those of morphological taxa in discrimination of the higher group, however, was not completely agree in the subgroup. The family Steinernematidae belong to Rhabditida was clarified as closer to the Tylenchida, rather than the other Rhabditida of Heterorhabitidae, Rhabditidae, and Diplogatroidae in genetic distance valule. From the result of the morphological classification and RAPD of the genomic DNA showed that genetic relationship analysis furnish information on phylogenetic classification and relationships of entomopathogenic nematodes. The application of genetic similarity will overcome the limitation of taxonomy and classification of morphologically simple nematode. Several primers were confirmed those utility of identification for individual nematode strains, the methods of molecular genetics secured the simplicity, rapidity and accuracy on the selection of entomopathogenic nematodes. [Nematode, Entomopathogenic, Biological control, RAPD].
담배가루이(Bemisia tabaci )에서 분리한 곤충병원성진균의 동정 및 병원성 검정
박현로,류영현,연일권,남성희,김동근,한명세,Park, Hyun-Rho,Ryu, Young-Hyun,Yeon, Il-Kyen,Nam, Sung-Hee,Kim, Dong-Geun,Han, Myung-Sae 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.1
자연 감염된 담배가루이로부터 곤충병원성진균을 분리 동정하고, 이 균주에 대하여 배양특성, 자연발병 요인, 그리고 주요 온실해충인 담배가루이, 점박이응애, 대만총채벌레에 대한 살충력을 검정하였다. 분리 균주의 형태적 특성은 직립한 분생자병에 윤생으로 호리병 형태의 4-6개 phialide를 지녔고, 분생포자의 크기는 $3.0-3.4{\mu}m$ 이었다. 분리 균주의 rDNA의 ITS영역 염기서열 분석에서 Isaria fumosorosea (AF461747)와는 99%의 높은 염기서열 유사도를 보였다. Potato dextrose agar, Sabouraud maltose agar+yeast extract 그리고 Sabouraud dextrose agar+yeast extract 배지에서 IFs 균주는 $24^{\circ}C$의 SDAY 배양에서 균사 성장이 가장 양호하였으며, $15^{\circ}C$보다는 $35^{\circ}C$의 고온에서 균사 성장이 좋았다. I. fumosorosea IFs-08, -09 균주는 담배가루이에 대하여 각각 96.7, 93.9%의 높은 살충율을 보였으며, 점박이응애(92.0, 84.9%) 그리고 대만총채벌레(84.4, 81.5%)에서도 80-90%정도의 살충율을 보인 반면 KACC로부터 분양받은 Isaria tenuipes, Isaria farinosa, Isaria fumosorosea 균주들은 10-20% 내외의 낮은 살충율을 나타내었다. Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.
우순옥(Soon-Ok Woo),여주홍(Joo-Hong Yeo),홍인표(In-Pyo Hong),한명세(Myung-Sae Han),백하주(Ha Ju Baek),정현주(Hyun-Ju Jung),김세건(Se-Gun Kim),장혜리(Hye-Ri Jang),한상미(Sang-Mi Han) 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.4
We study that their optimal extraction condition honey maesil (Prunus mume) extracts to health functional food were extracted with the honey. The extraction ration was in the condition of 1 maesil and 1.5 honey weight for 2 months. As standard ingredient, citric acid were determined 10.2mg/g in honey maesil extracts by HPLC. Cyan compound was not detected by the picrate method. Also Escherichia coli was not detected by 3M paper method. The honey maesil extracts contained free sugars as fructose and glucose (43.5%), and sucrose (1.5%). These results suggest that honey is the effective extracts method for the health functional food in maesil extracts.
Primary Cultures of Drosophila melanogaster Gut Cells for Studies of Intestinal Stem Cell Regulation
Young-Il Yoon(윤영일),Jae-Sam Hwang(황재삼),Tae-Won Goo(구태원),Myung-Sae Han(한명세),Mi-Young Ahn(안미영),Eun-Young Yun(윤은영) 한국생명과학회 2012 생명과학회지 Vol.22 No.5
초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄기세포가 작용하는지를 이해하기 위해 초파리 장세포의 일차배양 방법을 확립하였다. 초파리 성충으로부터 장을 해부하고 다양한 효소를 처리하여 장세포를 분리한 후 배양하였다. 배양세포의 생존여부는 현미경 검경 및 MTS assay에 의해 확인한 결과 배양 후 9일째 최대 증식되었고 14일까지 생존함을 확인할 수 있었다. 또한 장줄기세포 및 장내분비세포의 존재도 immunostaining에 의해 확인하였다. 따라서 본 연구에서 구축된 초파리 일차배양 장세포는 다양한 유전자에 의한 장줄기 세포 조절연구뿐만 아니라 장에서 발생하는 다양한 질병을 연구하는 도구로 매우 유용할 것으로 추측된다. Drosophila melanogaster has been used as a useful model to study development and disease. In this study, we established the primary culture method of Drosophila in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair during infection and disease. To obtain intestinal cells, we separated intestines from adult flies and isolated single cells by enzymatic treatment. The survival of cultured cells was measured using MTS-analysis. The maximum growth rate of the cells was observed on the 9th day after seeding. In addition, the presence of ISCs and enteroendocrine cells was confirmed by delta and prospero staining. Accordingly, we supposed that Drosophila melanogaster gut cells established in this study are probably useful in studies about intestinal stem cell regulation and various diseases occurring in the intestine.
동충하초 재배상 내 기형균 및 오염균의 발생현황 및 특성
남성희 ( Nam Seong Hee ),윤철식 ( Yoon Cheol Sik ),정이연 ( Jung I Yeon ),지상덕 ( Ji Sang Duk ),조세연 ( Cho Sae Yun ),한명세 ( Han Myung Sae ) 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.1
7개도 22개 재배농가로부터 수집한 시료는 총 529점이었으며, 수집균은 두 가지 유헝으로 구분되며 생육장애에 의한 기형균은 23.2%, 오염균은 50.9%였다. 생육장애는 C-1, C-2, C-3 및 C-4의 4가지 유형이 있으며 이들 분생자병속은 20∼70여 개가 발생되는데 회색, 순백색 또는 등황색을 띤다. 분생자병속 말단은 분지하지 않고 기주 표피에 각각 고유색상을 띠며 PDA 배지상에서 균사생장량은 14일간 43∼75 mm 범위로 눈꽃동충하초균보다 빠른 생장을 보인다. Occurrence and charaberistics of infectious fungi and physiologically abnormal Paecilomyces tenuipes were examined. These unfavorable fungi are recently known to cause yield loss, decreased quality of products in artificial cultivation farms of P. tenuipes. Total 529 fungal isolates were collected from 22 farms of 7 provinces. These isolates were composed of physilolgically abnormal P. genuipes strains and other infectious fungal strains, which constituted 23.2% abd 50.9%,k resoectuvekt. Physiologecally abnormal P. tenuipes strains showed irregular synemata,k and absence of local color. They were divided into 4 type of C-1, C-2, C-3,and C-4. Other infectious fungal strains were idevified to 5 species,Bearveria bassiana, Fusarium sp., P.fumosoroseus, Tricothecium roseum,k Aspergillus parasiticus,k which were 22.1, 13.8, 7.6, 40. and 3.4%, respectively. All of them were hyphomycetous fungi, did not produce synemata, and revealed faster growth rate than that of P. tenuipes.
절피원심법으로 대규모 채취한 누에체액의 곤충세포 증식효과
최지영 ( Ji Young Choi ),김종길 ( Jong Gill Kim ),최영철 ( Young Cheol Choi ),김삼은 ( Sam Eun Kim ),한명세 ( Myung Sae Han ) 한국잠사학회 2007 한국잠사곤충학회지 Vol.49 No.1
누에체액이 동물세포 배양의 필수첨가물인 FBS를 대체할 수 있는 세포증식 효과가 있는 것으로 확인되었으나, 누에의 배발에 상처를 내어 채혈하는 방법으로는 대량 채취가 어렵다. 본 연구에서는 이러한 문제점을 해결코자 대량의 체액을 한꺼번에 얻을 수 있는 대규모 채혈법을 검토하게 되었다. 1. 누에 머리부분과 꼬리부분 (배발 포함)에 상처를 낸 후 원심하여 체액을 채취하는 방법 (절피원심법)이 기존의 소규모 채취한 체액과 유사한 세포증식 촉진효과를 보였다. 2. 절피원심한 체액의 원심속도에 따른 세포증식 촉진 효과는 500 rpm과 1000 rpm에서 양호한 값을 보였다. 3. 원심속도를 500 rpm으로 고정한 경우 원심시간을 5분에서 30분까지 달리하여도 세포중식 효과는 유사하였고, 체액 회수율은 원심시간이 길어질수록 증가하였으나 일정시간 이상부터는 증가하지 않았다. 4. 절피원심법으로 대규모 채취한 체액의 액상회수율은 누에(백옥잠) 생체중의 19.5%이었고, 분말회수율은 1.4%이었으며, 분말체액에서도 세포증식 촉진 효과가 인정되었다. This study was carried out to investigate the utilization of hemolymph of silkworm, Bombyx mori, as a substitute for fetal bovine serum (FBS) in the insect cell culture. Hemolymph is collected on a small scale by clipping the abdominal leg; however, this method is not appropriate for large scale collection. The hemolymph was collected from 5 th instar larva by centrifugation after cutting of the abdominal legs was more appropriate procedure for large scale collection. The cell growth in the medium supplemented with hemolymph (Baekokjam) collected in large scale was almost same as that in the medium hemolymph supplemented with hemolymph collected in small scale. However, the mutant (wEb) hemolymph collected in large scale was still less effective in the cell growth, as compared to the Baekokjam hemolymph collected in large scale. The optimum centrifugation condition for large-scale bleeding was 500 rpm and 15 mm.