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한면수,정동효,Han, Myun-Soo,Chung, Dong-Hyo 한국식품과학회 1985 한국식품과학회지 Vol.17 No.4
부채전분원에서 분리, 동정한 Aspergillus niger를 사용하여 koji를 조제하고 생전분 당화와 에탄을 발호의 조건을 검토한 결과는 다음과 같다. (1) Koji(효소제)는 4일간 배양한 경우에 가장 높은 활성을 가졌고 생전분 당화의 최적pH는 3.3. 최적온도는 $40{\sim}50^{\circ}C$ 이었다. (2) 전분별에 따른 당화는 옥수수전분. 고구마전분. 감자전분의 순위였다. (3) 알코올 발효시 전분의 최초 담금농도는 $20{\sim}30%$이었다. (4) 전분원료중 쌀, 고구마, 옥수수에서 무증자전분의 알코올 발효가 가능함을 보였으나, 증자한 전분들 보다 발효종료일은 약 $1{\sim}2$일 지체되었다. (5) 무증자에 의한 전분의 당파와 동시 알코올 발효에서 $30^{\circ}C$. pH3.3, 100rpm 왕복진탕 발효시 최적발효일은 5일, 쌀, 고구마, 옥수수전분에서 최종 에탄올수율은 약95%이었다. The energy for cooking starch prior to saccharification could be saved by fermenting raw starch into ethanol using Aspergillus niger koji of wheat bran. Optimum cultivation time to produce glucoamylase was 4 days in wheat bran medium. The rate of saccharification from uncooked corn starch were optimum at pH 3.3 and 40-$50^{\circ}C$. Corn and sweet potato starch were saccharified more efficiently by wheat bran koji than other tested starch sources. 5 days of fermentation were required for optimum yield of ethanol using a mixture of AspergiUus niger koji and dried yeast. Final ethanol yields from raw corn, sweet potato, and rice starch with agitation at the rate of 100 rpm were about 95% at $30^{\circ}C$.
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특집자료 : 경찰 초동수사 목적의 유전자감식정보 검사 및 관리
한면수 ( Myun Soo Han ) 한국형사정책학회 2006 刑事政策 Vol.18 No.2
In forensics, the criminal genetic studies have become a usual tool, due to the possibility of very small biological stains identification. DNA evidence has taken a central role by carrying a significant weight for convictions, as well as by excluding innocent suspects early on in a criminal investigation. The DNA amplification by the polymerase chain reaction(PCR) technique supported very strongly the development of forensic genetics, specially the criminalistics. Forensic DNA databases on purpose of primary criminal investigation have been created in several countries: the United Kingdom was the first European country to have, in 1995, a DNA database. Subsequently, the U.S.A.(1998) and many others have introduced or are preparing databases. Different national legal systems have conditioned the DNA databases and so there is a great heterogeneity between countries. The criteria for an entry of a DNA profile, the criteria for a removal, the criteria for a search, etc., can lead to very distinct databases. The DNA profiling systems are standardized based on generally accepted recommendations from scientific bodies such as the National Research Council (NRC) of the Academy of Sciences of the United States, and the International Society for Forensic Genetics (ISFG). The STR technology, currently used to form the databases, has no apparent value in giving genetic information other than for individuality. However, it is not only the results of the DNA analysis which are retained. The samples, which are provided for the analyses, can also be stored in case there is a need for re-analysis in a particular case or because there is a change in the technology whereby all accumulated samples can be re-examined. The storage of forensic database is an important question too and it is possible to choose between the storage of only the DNA profiles or also the biological samples. Presently, we will be legislate for the law for DNA identification. The aim of this research is to provide objective data that may serve to aid the drawing up of future database regulations according to the true situation of the forensic sciences in Korea.
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한면수(M . S . Han),한성준(S . J . Han),김영진(Y . J . Kim),황성구(S . K . Hwang),고태송(T . S . Koh) 한국축산학회 1991 한국축산학회지 Vol.33 No.9
In order to investigate an effect of domestic barley on the carcass characteristics of pigs, three way cross bred (Landrace × Large white × Hampshire) pigs fed on a commercial diet until about 76㎏ were allotted to 20 pigs per pen and three pens per diet. And then they fed on basal diet and diets in which 10%(l0% barley diet) or 20%(20% barley diet) of the domestic barley substituted for yellow corn and soy bean meal of the basal diet during 38 days of experimental feeding for finishing period. Digestibility of nutrients and energy of diets were measured. And dressing percentage, loin eve area, backfat thickness at site of 7th, 10th and last ribs and amount of lean meat of carcass were measured on 45 pigs chosen randomly by 5 pigs per pen after experimental feeding. During experimental feeding period body weight gain and feed conversion did not show significant differences among pigs fed diets of basal and 10% barley, while pigs fed 20% barley diet tended to decrease body weight gain and worsened feed conversion. Pigs fed diets containing 10% or 20% barley showed the increased digestibilites of crude protein and nitrogen-free extracts. But digestibility of crude fiber in pigs fed 20% barley diet decreased. Basal diet contained 3.001 Meal DE per ㎏ dry matter, and 10% barley diet and 20% barley diet contained 3.252 and 3.240 Meal, respectively. Also digestibility of energy was heightened significantly(p$lt;0.05) in diets containing barley compared with that in basal diet. Dressing percentages, and lean meat percent tended to be high in pigs fed 20% barley diet compared with those of pigs fed basal and 10% barley diets. Average backfat thickness of pigs fed 20% barley diet was 2.59 ㎝ which was clearly thinner compared with 2.82 and 2.73 ㎝ of pigs fed basal and 10% barley diets, respectively. The results indicated that pigs fed diets containing 20% of the domestic barley increased dressing percent, loin eye area and lean meat percent, and decreased backfat thickness compared with those of pigs fed basal deit. It was suggested that the domestic barley will possibly improve the market carcass quality of pigs.
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한면수(Han, Myun Soo) 경찰대학 경찰학연구편집위원회 2015 경찰학연구 Vol.15 No.2
Today DNA is one of the most highly regarded tool available to the forensic scientist and the DNA profiles obtained from crime scene material are compared with those of known potential suspect and volunteers(i.e. DNA screening). The scope of the proposed DNA database must be limited to crime investigation. Recent changes in legislation have begun to allow for the possibility to expand the parameters of DNA database searches, taking into account the prediction of surname, racial profiling, familial kinships, and EVCsExternally Visible Characteristics et al. DNA mass screening can be carried out in cases where no DNA profile match is obtained and no other evidence is available. In such DNA dragnets, larger number of volunteers(hundreds to thousands), usually those living in the geographic region where the crime occurred, are invited to voluntarily provide a saliva sample for STR profiling. Using conventional autosomal STR profiling in the DNA dragnet limits the possibilities of another purpose search to close relatives of the unknown, non-participating perpetrator. Since Y-STR profile identifies a man together with all his paternal male relatives, such as surname, and then a Y-STR dragnet is more effective. DNA phenotyping refers to the prediction of appearance traits of unknown sample donors directly from biological samples found at the criminal scene. “Biological witness” outcomes of forensic DNA phenotyping can provide investigative leads to trace unknown persons, who are unidentifiable with current comparative DNA profiling. Because of the Information on the dynamic of the human genome, including its surname, racial profiling, familial kinships, and EVCs, which has several advanced since such laws were made, and should therefore not be use of DNA shall be regulated on the particular forensic purpose but not the type of markers that likely are changing over time with increasing genetic knowledge.
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