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cPCR 기법을 이용한 초기배양 pH에 의한 반추위 섬유소 분해 박테리아의 부착 및 발효에 관한 연구
김민석,성하균,김현진,이상석,장종수,하종규,Kim, M.S.,Sung, H.G.,Kim, H.J.,Lee, Sang-S.,Chang, J.S.,Ha, J.K. 한국축산학회 2005 한국축산학회지 Vol.47 No.4
본 연구는 배양초기 pH 조건이 F. succino- genes의 섬유소 부착과 섬유소 소화에 미치는 영향을 보고자 실시하였다. 선정된 specific primer를 이용하여 F. succinogenes의 genomic DNA로부터 445bp의 16S rDNA 절편을 증폭하여 205bp의 internal control을 제작하였고, cPCR 결과로부터 박테리아 수를 계산할 수 있는 표준곡선의 회귀식($r^2$>0.99)을 얻을 수 있었다. In vitro 배양초기 pH에 따른 F. succ- inogenes의 cellulose 부착을 cPCR로 모니터링한 결과, 발효과정 전 기간동안 초기 pH가 6.8과 6.2일 때 cellulose 건물 g당 부착 균주의 수가 pH 5.6일 때 보다 높았으나, pH 6.8과 6.2 사이에서는 큰 차이는 없었다(p>0.05). Cellulose 분해는 배양시간이 진행됨에 따라 증가 되었으며, 분해 정도는 pH가 증가함에 따라 더 높았다. 배양초기 pH가 6.8, 6.2 그리고 5.8일 때 48시간동안 감소한 pH는 각각 0.24, 0.58 그리고 0.16 이었다. 가스 생산량은 발효 시간이 경과함에 따라 pH가 높을수록 더 많았다. 결론적으로 발효 초기 pH는 F. succinogenes에 의한 cellulose 소화, 가스 생산, pH 변화 및 cellulose 부착에 큰 영향을 주었으며, 특히, 낮은 pH(5.8)에서는 섬유소 소화 및 박테리아 부착을 현저한 감소 시켰다. The cPCR technique was used to monitor rumen fermentation and attachment of Fibrobacter succinogenes to cellulose at different pH in the in vitro culture medium. The target fragments of 16S rDNA(445 bp) were amplified from genomic DNA of F. succinogenes with specific primers and internal controls(205 bp) were constructed. Cell counts were estimated from the amounts of genomic DNA, which was calculated from cPCR results. F. succinogenes in pH 6.8 and 6.2 showed apparently higher attachment than in pH 5.8 during all incubation time. There were some difference between pH 6.8 and 6.2 in the degree of attachment, but the different was not significant (P>0.05). Cellulose degradation increased in process of incubation time and the increasing rate was higher when initial pH was higher. The pH in culture medium decreased regardless of initial pH in course of incubation time. After 24 h of incubation, medium pH was dropped by 0.24, 0.58 and 0.16 units from original medium pH 6.8, 6.2 and 5.8, respectively. More gas was produced at higher initial pH in the same manner as in cellulose degradation. In summery, Initial pH of rumen culture in vitro significantly influenced cellulose digestion, gas production, pH change and bacterial attachment. Especially, low pH(5.8) resulted in much lower bacterial attachment and fiber digestion compared to higher medium pH.
볏짚의 사료가치 증진을 위한 적정 알카린 H2O2 처리 수준에 관한 연구
문양수(Y . S . Moon),하종규(J . K . Ha),고종열(J . Y . Ko),최연호(Y . H . Choy),조경훈(G . H . Cho),최윤재(Y . J . Choi),한인규(I . K . Han) 한국축산학회 1990 한국축산학회지 Vol.32 No.10
This study was carried out to determine the adequate levels of alkaline hydrogen peroxide treatment for the improvement of nutritive value of rice straw. In vitro digestibility and chemical analysis after several treatments were measured. Treatment variables were soaking time(12, 24, 48 and 96 hrs), temperature(5, 25, 50 and 75℃) of alkaline H₂0₂ solution, H₂0₂ concentration (0.5, 1, 2, 3 and 4%) and substrate /solution ratio (1, 2, 3, 4 and 5g/ml). The results obtained were summarized as follows. 1. The DM digestibilities or rice straw treated with H₂O₂ for 24 and 48 hours were higher than those of the others(p$lt;0.05). There was no differ ences when the rice straw was treated for 12, 72 or 96 hours. Cell wall contents were not affected by the time of treatment. 2. The DM digestibilities were higher at pH 11.5 or above(p$lt;0.05) and was decreased as pH declined. When rice straw was treated with H₂O₂ at pH 11.5 or higher, NDF, ADF and cellulose contents were increased. However, lignin content and DM recovery percentages were decreased. 3. The DM digestibilities were not influenced by the ratio of straw versus liquid. The contents of NDF and cellulose were not affected up to the ratio of 4g rice straw/50m1, but were decreased at 5g rice straw/50m1. Lignin content and DM recovery percentages, however, were increased at 5g rice straw /50m1. 4. The DM digestibilities were not different among treatments at 5, 25 and 50℃. However, the rice straw treated at 75℃ showed lowest digestibility of all treatment (p$lt;0.05). The content of NDF, ADF and cellulose was increased by increasing temperature. However, lignin content and DM recovery percentages were decreased by increasing temperature. 5. The AHP treated rice straw had higher content of ADF and cellulose, and lower content of hemicellulose and lignin as the concentration of H₂O₂, increased. But hemicellulose, lignin and DM recovery percentages were decreased. The NDF content was not different among five different concentrations of H₂0₂ solution. In conculsion, based upon the results of present experiments the most desirable method is to soak rice straw in 1% alkaline H₂0₂ solution at pH 11.5, at room temperatrve (25℃), for 24∼48 hours and at the ratio of 4g rice straw /50m1 solmtion.
뇌병변 소아환자의 기립보조기 사용 시 접촉 패드 유형에 따른 근활성도 비교
이종훈(J. H. Lee),오민우(M. W. Oh),하종규(J. G. Ha),서재용(J. Y. Seo),황화식(H. S. Hwang) 한국재활복지공학회 2014 재활복지공학회논문지 Vol.8 No.3
본 연구에서는 Pneumatic air fitting system을 적용한 기립보조기 제품을 뇌병변 소아환자를 대상으로 기립보조기의 신체 고정용 폼패드(foam pad)와 에어패드(air pad) 사용 시, 뇌병변 장애아동들의 체간 및 하지근육의 수축 양상을 비교·분석하였다. 연구결과, 패드(foam pad vs. air pad) 유형에 따른 근육별 근력 활성도는 차이가 있었다. 이러한 차이는 큰 동작(식사동작)에서 크게 나타나고, 작은 동작(쓰기동작, 읽기동작)이 작게 나타났다. 이는 Pneumatic air fitting system을 적용한 기립보조기 제품 사용 시, 기존 폼패드에 비해 사용자에게 좀 더 편안함을 제공하는 것으로 나타났다. This study conducted a comparative analysis of the contraction pattern of trunk and lower limbs muscles of handicapped children with brain lesions during the use of foam pad and air pad for fixing a standing table to the body for pediatric patients with brain lesions using a standing table product applied with a pneumatic air fitting system. There was a difference in the measurement of muscle strength activity by muscle in accordance with pad type (foam pad vs. air pad). A large difference was found with big movements (movements of eating), while a small difference was discovered with small movements (writing and reading movements). This was found to furnish a little more comfort to users compared with the existing foam pad during the use of a standing table product applied with a pneumatic air fitting system.
대맥사일리지 및 대맥펠렛 급여가 젖소의 유량 , 유성분 , 반추위내 발효에 미치는 영향
김준식(J . S . Kim),이현준(H . J . Lee),김광종(K . G . Kim),조윤연(Y . Y . Cho),지설하(S . H . Chee),하종규(J . K . Ha) 한국축산학회 1987 한국축산학회지 Vol.29 No.11
The current study was undertaken to determine the feeding value of the whole-plant barley silage harvested at dough stage (BDS), barley silage harvested at ripening stage (BRS) and barley pellet (BP) as a roughage source for milking cows compared with corn silage (CS) and hay. Sixteen lactating cows (613.8㎏) producing 21.3㎏ of milk daily were alloted into four treatments. Digestrbility of various nutrients contained in three silages and one pellet, volatile fatty acid (VFA) concentration in rumen fluid after feeding these silages and pellet were investigated using twelve Corriedale rams. The results obtained were summarized as follows: 1. Milk production was significantly (P$lt;0.05) higher in BP group (21.24㎏) and BDS group (19.48㎏) than CS group (17.37㎏) and BRS group (17.80㎏). 2. There was no significant effects of diet on milk fat, but milk protein content was significantly higher (P$lt;0.05) in CS group and BRS group than BP group and BDS group. Lactose content was significantly increased (P$lt;0.05) when CS was fed compared with BDS group. 3. Differences in feed intake between CS group and the other groups were not significant. 4. Corn silage tended to increase body weight gain over other treatments, but there was no significant difference between four treatments. 5. The apparent digestibilities of roughage dry matter (DM) and crude protein were not different among treatment groups. 6. Ruminal total VFA production, molar percentage of rumen acetate, propionate, isovalerate were significantly higher (P$lt;0.05) in BRS group, but butyrate and valerate were not significant. 7. The feed cost far producing 1㎏ milk was estimated as ₩146 in CS group and ₩119 in BDS group. Higher net income (₩1486/head) was made in BP group.
브로일러에 있어서 무기태유황 , 메치오닌과 콜린간의 상호 관계에 대한 연구
고영길(Y . G . Ko),하종규(J . K . Ha),한인규(I . K . Han) 한국축산학회 1987 한국축산학회지 Vol.29 No.4
In order to study the interrelationship of dietary methionine, choline and inorganic sulfate, a 2 × 2 × factorial experiment was conducted with two levels of dietary methionine (0.34%, 0.5%), two levels of dietary choline (1,180㎎/㎏, 2,180㎎/㎏) and two levels of dietary sodium sulfate (0.18%, 0.276`0) using a total of 320 broiler chicks for a periods of 28 days. The results obtained from the present study are summarized as follows. 1. The growth response was obtained by supplementing the basal diet with methionine, choline or sodium sulfate alone (p$lt;0.05). The methionine × choline × sodium sulfate interaction was significant (p$lt;0.01). Supplementing the basal diet with sodium sulfate alone (equimolar sulfur) was as effective as that achieved by the addition of methionine alone. It may be suggested that methionine could be partially replaced by inorganic sulfate. 2. There was no increase in feed intake from the supplementation of methionine, choline or sulfate to the basal diet (p$gt;0.05). 3. Although there was no significant difference (p$gt;0.05), supplemental methionine or choline tended to improve protein utilizability compared to supplemental sodium sulfate alone. The diet without supplemental methionine was lower in protein utilizability than those supplemented with 0.29% methionine. Methionine × sodium sulfate interaction was significant (p$lt;0.05). 4. Supplemental sodium sulfate increased liver weight, but supplemental choline or methionine did not. Methionine × choline × sodium sulfate interaction was significant (p$lt;0.05). 5. Supplemental methionine or choline but no sodium sulfate reduced the fat content in the liver (p$lt;0.05) compared to control group. Methionine × choline, methionine × choline × sodium sulfate interactions were significant (p$lt;0.05). 6. Supplemental choline resulted in numerical increase in sulfur content in the liver. Sulfur in the form of methionine and sodium sulfate were deposited equally in the liver when equimolar sulfur was supplemented.