bFGF, PDGF-BB가 백서 골수기원 간엽 줄기세포의 조직골세포 분화에 미치는 영향에 관한 연구

송진아(Gin-Ah Song),최진영(Jin-Young Choi) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.6

In this study we evaluate the effects of bFGF-BB and PDGF on in vitro proliferation, differentiation and mineralization of mesenchymal stem cells(MSCs) from rat. MSCs were prepared from the bone marrow of 6 or 7-week-old male rats with a technique previously described by Maniatopoulos et al. in 1988. Lineage differentiation to osteogenesis, chondrogenesis and adipogenesis were performed. At first, we characterized the cultured cell on passage 1, 3,5, 7 with immunocytochemical staining using CD29, 44, 34, 45, α-SMA and type I collagen. And to study the effects of bFGF and PDGF-BB on proliferation, differentiation and mineralization, we seeded the expanded cell at a density of 6 × 103 cells/cm2 to 100-mm dish for evaluation of cell proliferation and MTT assay was carried out on day 2, 4, 7, 9. We also resuspended the cells with same density (6 × 103 cells/cm2) to 24 well plates for subculture. On the following day, the attached cells were exposed to 2.5ng/ml bFGF and/or 25ng/ml PDGF-BB daily during 5 days. The osteocalcin(OC) level was assessed and mineral contents were evaluated with alizarin red S staining on subculture day 2, 7, 14, 21. We identified the mesenchymal stem cell from the bone marrow derived cells of rat through their successful multi-differentiation and stable display of its phenotype. And bFGF and PDGF-BB showed the effect that inhibited osteoblastic differentiation and mineralization mildly in above concentration at in vitro culture. This study was supported by grant 04-2004-0120 from the Seoul National University Hospital Research Fund.

구강악안면외과 의료행위 상대가치 개발에 대한 조사연구

송진아(Gin-Ah Song),백경원(Kyung-Won Baek),황종민(Jong-Min Hwang),유순용(Soon-Yong Yu),최진영(Jin-Young Choi) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.4

The aim of this study is to evaluate the reasonableness of the medical fee on oral and maxillofacial surgery field according to surgeon’s opinions and actual conditions. The medical fee has significant influence on hospital income, the supply and distribution of medical manpower, quality and facilities of medical services. Questionnaire survey was sent to 86 oral and maxillofacial surgeons who worked more than 3 years in general hospital. Among them, 25 doctors replied the 109 answers survey and the average of treatment time and physician work relative value on each category was calculated. And the health insurance cost (that has been applied since 2003) was compared with the questionnaire results. And finally we investigated items that health insurance system did not include in oral and maxillofacial field but actually performed in oral and maxillofacial surgery clinic. The result was that the medical fee did not properly reflect physician work relative value of actual treatments. In case of complicated extraction, work relative value needed 3.5 times enhancement of present value. For simple impacted tooth extraction 1.8 times, for impacted tooth extraction including odontomy 1.7 times, and for fully impacted tooth more than 2/3 of it located into the alveolar bone, 1.8 times enhancement needed. In respect of the present physician work relative value, hemimandibulectomy with neck lymph node dissection for the malignancy is appropriated as 3.3 times of open reduction and internal fixation for the mandibular fracture, but the questionnaire result showed 25 times discrepancy. In conclusion, this research shows the need for intervention that health insurance included items and legal relative medical value must act in union with treatment in clinic to reduce the imbalance between them.

유질개선(乳質改善)을 통한 락농가(酪農家) 소득증대(所得增大): SCC와 유방염(乳房炎)을 중심으로

손봉환 ( Bong Whan Sohn ),최진영 ( Gin Young Choi ),배도권 ( Do Kown Bae ),정충일 ( Chung Ill Chong ) 한국동물위생학회 1997 한국동물위생학회지 (KOJVS) Vol.20 No.3

The study for a effect of monitoring on bovine mastitis was conduced for improvement of raw milk from Jan. to Dec. in 1996. Sampling the milk of 367 cows(1,406 quarters) from 5 herds in Inchon and were carried out California mastitis test(CMT), somatic cell count(SCC), isolation of pathogens and antibiotic sensitivity tests. The results were summarized as follows, 1. The number of bovine mastitis was 177 cows(48.2%) and 371 quarters(26.4%); clinical mastitis: 25 cows(6.8%), 32 quarters(2.3%) and subclinicsl mastitis: 152 cows(41.4%), 339 quarters(24.1%). Incidence rate of mastitis by season were Summer 52.0%, Fall and Winter 48% and Spring 41%. Incidence rate of mastitis by quarters were Summer 30%, Fall 28%, Winter 25% and Spring 21%, respectively. 2. In the distribution of CMT degree by quarter, CMT positive(CMT±) of 1,406 quarters milk were 50.1%(704 quarters). The ratio of CMT positivity by quarter were left front quarter 55.8%, right front quarter 48.9%, right hind quarter 48.6% and left hind quarter 47%. The ratio of CMT positivity by season were Summer 54.1%, Fall 49.7%, Spring 48.5% and Winter 48%. 3. The highest mean SCC by season among 5 herds was “A” herd. Mean SCC (cell/ml) ofA herd were Summer 2,032,000cells/ml, Fall 1,109,000cells/ml, Winter 782,000cells/ml and Spring 577,000cells/ml. The lowest mean SCC by season among 5 herds was “E” herds. Mean SCC of E herd were Summer 1,064,000cells/ml, Spring 795,000cells/ml, Fall 429,000cells/ml and Winter 400,000cells/ml. Mean SCC of the other herds by season were little difference. 4. The milk samples of “A” herd were collected from 10 cows. In 3 seasons, mean 5CC of No. 2 and 3 cows were than 1,000,000cells/ml. In 1 season, mean SCC of No. 6, 7 and 8 cows were than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Summer 4 cows, Winter 3 cows, Spring and Fall 1 cow respectively. The milk samples of “B” herd were collected from 14 cows. In 3 seasons, mean SCC of No. 1 cow was more than 1,000,000cells/ml. In 2 seasons, mean SCC of No. 5,9 and 14 cows were more than 1,000,000cells/ml. In 1 season, No. 3,6 and 7 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Fall and Winter 4 cows respectively, Summer 3 cows and Spring 1 cow. The milk samples of “C” herd were collected from 18 cows. In 2 seasons, mean SCC of No. 16 cow was more than 1,000,000cells/ml. In 1 season, mean SCC of No. 1, 2, 6, 7, 13, 15 and 18 cows were more than 1,000,000cells/ml respectively. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Summer 5 cows, Fall 3 cows, Spring 2 cows and Winter 1 cow. The milk sampes of “D” herd were collected 24 cows. In 3 season, mean SCC of No. 14 cow was more than 1,000,000cells/ml. In 2 seasons, mean SCC of No. 14 and 18 cows were more than 1,000,000cells/ml. In 1 season, mean SCC of No. 1, 2, 3, 8, 12, 17, 19, 20 and 21 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows were distributed Fall 15 cows, Spring and Winter 4 cows respectively and Summer 3 cows. The milk samples of “E” herd were collected from 27 cows. In 2 seasons, mean SCC of No. 6, 7 and 21 cows were more than 1,000,000cells/ml. In 1 season, mean SCC of No. 2, 4, 7, 11, 14, 16 and 23 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows were distributed Spring and Fall 5 cows respectively, Summer and Winter 2 cows, respectively. 5. The rate of isolated pathogenic microorganisms from bovine mastitis were summarized as follows: Staphylococcus sp 168 strains(45.8%), Streptococcus sp 82 strains(22.3%), Gram(-) sp 45 strains(12.3%), Gram(+) sp 51 strains and the other sp 21 strains(5.7%). 6. The highest of antibiotic sensitivity test of each microorganism was summarized as follows: Staphyolcoccus sp-cephalosporin 76%, gentamicin 55%, Streptococcus sp-ampicillin 61%, cephalosporin 63%, Gram(-) sp-gentamicin 58%, Gram(+) sp-cephalosporin 63%, The other sp-cephalosporin 90%. Microorganisms showed the highest sensitivity(68%) to cephalospsorin.

하악골에 발생한 Gorham-Stout 증후군: 증례 보고

지영민,송진아,신정현,방강미,김성민,명훈,서병무,최진영,이종호,정필훈,김명진,황순정,Ji, Young-Min,Song, Gin-Ah,Shin, Jung-Hyun,Pang, Kang-Mi,Kim, Soung-Min,Myoung, Hoon,Seo, Byoung-Moo,Choi, Jin-Young,Lee, Jong-Ho,Choung, Pill-Hoon,Kim, 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.6

Gorham-Stout 증후군은 조직학적으로 양성의 혈관 증식을 보이면서 광범위한 골 용해 현상을 나타내는 희귀한 병으로 알려져 있다. 병인론은 아직 명확히 밝혀져 있지 않으며 주로 남성과 젊은 성인에게서 호발하는 것으로 알려져 있다. 진단에는 컴퓨터 단층촬영, 자기공명영상, 핵의학검사, 혈관조영검사 등의 영상검사가 도움이 되며, 조직학적검사 역시 몇몇 특이 소견을 통해 진단에 도움을 줄 수 있다. 발생하는 부위에 따라서 그 예후는 매우 다르며, 특히 척추나 흉곽에 발생할 경우는 생명을 위협할 수 있다. 저자등은 우측 하악골의 병적 골절을 주소로 내원한 환자에서 임상검사, 방사선학적 검사, 조직병리학적 검사를 통해 Gorham-Stout 증후군으로 진단한 환자를 비골유리피판을 이용한 하악골 재건술을 통해 치료하였고 4년 동안 관찰 기간 중 재발 없이 양호한 치유 경과를 보였기에 문헌고찰과 함께 보고하는 바이다. Gorham-Stout disease is a rare skeletal disease which is progressive and spontaneous and idiopathic resorption of whole body bone. In the initial stage, the osteolytic site is replaced by histologically benign vascular proliferation without new bone formation and finally by dense fibrous tissues. We encountered with a patient involving the mandible with attention by the clinical, radiographic, scintigraphic, angiographic, and computed tomography. We resected the mandibular lesion which was reconstructed with a fibular free flap. We report literature review with a mandibular lesion involved with Gorham-Stout disease.

Letterer-Siwe씨 병의 1례 보고

김소연,유병훈,최진영,심태섭 중앙대부설한국의과학연구소 1975 한국의과학 Vol.7 No.2