Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

연안지역 화학공장부지의 부지환경평가를 위한 복합조사기법의 적응

최승진,우남칠,Choi, Seung-Jin,Woo, Nam-Chil 대한자원환경지질학회 2007 자원환경지질 Vol.40 No.4

최근 국내에서는 외국기업의 국내진출에 따른 M&A, 미국과의 자유무역협정에 따른 시장개방에 따라 부지환경평가 (토양환경평가)의 결과를 반영할 필요성이 점차 증가되어 왔다. 따라서 이 연구에서는 연안매립지역에 위치한 대규모 화학공장부지에 대한 실제적인 부지환경평가를 통하여 국내의 토양환경평가 지침의 문제점을 보완하고 효율적, 경제적 평가를 위한 복합적 조사기법들을 이용하였다. 이 연구는 기초조사, 부지현황조사, 시료채취와 분석, 관측정 설치와 수리지질 조사, 자료 분석의 6단계로 진행되었다. 각각의 조사단계에서는 기존자료조사, 선구조분석, 지표지질조사, 지구물리탐사, 시추조사, 수리전도도, 지하수의 유향유속측정, 시료채취 및 분석 결과를 조합하여 복합적으로 해석하였다. 연구결과, 부지환경평가를 위한 이러한 다양한 조사기법들의 적용은 부지의 오염현상, 지하매질에 대한 부지특성화에 매우 효과적이었다. 국내에서는 '토양환경평가 지침'에 의한 기초조사에 있어 기존자료의 이용, 가용한 자료의 보관상태, 평가를 위한 관계자 인터뷰 등에 있어 매우 열악한 여건을 가지고 있다. 따라서 '부지환경평가'를 수행하는데 있어 이러한 복합조사기법의 적용은 더욱 경제적이고 효율적이며, 토양과 지하수오염을 연계하여 평가하는데 있어서도 매우 유용할 것으로 기대된다. Recently, the branch-out of foreign companies into domestic markets through M&A and the opened followed by the Free Trade Agreement(FTA) with America have made the environmental site assessments of specific site more necessary. In this study, through case study of conducting actual environmental site assessment by use of a toolbox approach at a large scale of chemical plant with various contaminants located in a coastal area, the problems of guideline of domestic environmental assessment of soil were complemented. And an efficient and economical assessment was achieved. All six steps such as basic investigation, environmental site history survey, sampling and analysis, installation of monitoring wells and hydrogeological survey, and data interpretation were conducted in this study. All results of document survey, geological lineament analysis, field geology survey of surrounding area, geophysical prospecting of the site, hydraulic conductivity, measurement of groundwater flow rate and direction, sampling and analysis at each step were associated and estimated as an integrated tool box approach. As a consequence of this study, toolbox approaches were very useful techniques for contamination level and site characterization of subsurface media. The given conditions to conduct a basic survey for domestic soil environment assessment of site by use of existing documents, as well as interviews with the owner/manager/user of all adjacent properties and thorough review of all practically reviewable records pertaining to the property and surrounding properties within "Guideline for Soil Environment Assessment" radii are very poor. As a result, the application of toolbox approach in the environment site assessment of site is not only more efficient and economical, but also could be very useful assessment to integrate the soil and groundwater contamination.

파킨슨병 모형 흰쥐의 줄무늬체에서 Apomorphine 투여 방법에 따른 도파민 D2 수용체의 발현

최승진,성재훈,손병철,박춘근,권성오,김문찬,이상원,Choi, Seung Jin,Sung, Jae Hoon,Son, Byung Chul,Park, Choon Keun,Kwon, Sung Oh,Kim, Moon Chan,Lee, Sang Won 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.7

Objective : Parkinsonian rat models have generally been characterized by unilateral destruction of both the nigrostriatal pathway and the mesolimbic pathway using the neurotoxin 6-hydroxydopamine. The induction of contraversive turning by apomorphine in these models is thought to reflect the stimulation of supersensitive dopamine D2 receptor or receptor-mediated mechanisms in denervated neostriatum. The present study was undertaken to investigate the expression of dopamine D2 receptor in denervated striatum according to modalities of apomorphine(dopamine agonist) treatment after creating a hemiparkinsonian rat model in which there is 6-hydroxydopamine induced destruction of the unilateral dopaminergic nigrostriatal pathway. Methods : After making complete lesion in left side substantia nigra pars compacta(SNpc) by stereotactic injection of 6-hydroxydopamine into medial and lateral areas of SNpc, and confirming successful animal model by apomorphine induced contraversive turning behavior without recovery and complete destruction of ipsilateral SNpc with tyrosine hydroxylase immunostaining in 7th day after operation, 15 rats of parkinsonian model were studied with or without administration of apomorphine at varying doses and durations. According to the modalities of apomorphine treatment for 4 days, these rats were divided into 3 groups, as not-treated group, intermittently treated group and constantly treated group. For investigating the extent of the expression of dopamine D2 receptor in denervated striatum, immunohistochemical staining by dopamine D2 receptor antibody and Western blot were performed. Results : In the D2 receptor antibody immunohistochemical staining, the mean number of positive stained neurons was highest in not-treated group($20.5{\pm}1.14$) of 3 groups. In constantly treated group, the mean number of positive stained neurons was less($3.9{\pm}1.79$) than intermittently treated group(p<0.05). The Western blotting with the D2 receptor antibody revealed that expression of receptors was also highest in not-treated group and less in constantiy treated group than intermittently treated group. Conclusion : Dopamine D2 receptors in denervated striatum of parkinsonian rat models, which were not treated with apomorphine, revealed to be most highly expressed. And, according to doses and durations of apomorphine administration, desensitization of the receptor was more apt to develop with constant treatment than intermittent treatment. In clinical setting, the authors believe that, in long-term treated parkinsonian patients, desensitization of dopamine receptors due to chronic dopaminergic stimulation seems to be partially related to mechanisms of drug tolerance.

Neural Learning Algorithms for Independent Component Analysis

최승진,Choi, Seung-Jin Institute of Korean Electrical and Electronics Eng 1998 전기전자학회논문지 Vol.2 No.1

Independent Component analysis (ICA) is a new statistical method for extracting statistically independent components from their linear instantaneous mixtures which are generated by an unknown linear generative model. The recognition model is learned in unsupervised manner so that the recovered signals by the recognition model become the possibly scaled estimates of original source signals. This paper addresses the neural learning approach to ICA. As recognition models a linear feedforward network and a linear feedback network are considered. Associated learning algorithms for both networks are derived from maximum likelihood and information-theoretic approaches, using natural Riemannian gradient [1]. Theoretical results are confirmed by extensive computer simulations.

Military Personnel’s Social Relationships Analyzed by their Profiles of Behavioral, Emotional, and Cognitional Characteristics

최승진(Seung-jin Choi),이준희(June-hee Lee),이양(Yang Lee) 영곡사회심리발달학회 2023 사회심리발달연구 Vol.4 No.1

Objectives This study has the purpose of determining that how the social relationships in the Korean military are figured out by the profile of behavioral, emotional, and cognitional characteristics. Methods Data were collected from 54 military personnel by the phenomenological observation and experimental scales. Results The results of this study showed that the typical military personnel were higher activated than the atypical at all characteristic profiles. More analytically, the emotional characteristic is a boundary of typicality; the higher the emotion, the more typical it is. Conclusions This study suggests that management of emotional activation would be regarded as boosting military capability, and the limitations of this study require comparisons between the military and general societies.

부자유친성정이 기독교 청소년의 자기표상과 하나님 이미지에 미치는 영

최승진 ( Seung Jin Choi ) 한국복음주의상담학회 2013 복음과 상담 Vol.21 No.-

The purpose of this the study is to examine what effects is exerted on self representation and God`s image by parent-adolescent affective bonding(bu-ja-yu-chin-sung-cheong) as perceived by Christian adolescents. To achieve such purposes of the study, recognition on parent-adolescent affective bonding of Christian adolescents, self representation along with God image were analyzed to investigate the differences and their influence on God`s image and self representation. The research was performed for 856 both male and female Christian adolescents (from 1st grade of junior high to 3rd grade of high schools). The results are as follows:First, according to the verification results of hypotheses for the influence of parent-adolescent affective bonding on self representation, it was found that sense of unity and dedication as variables related to parent-adolescent affective bonding have statistically significant correlation with sociability, benevolence and competence as variables related to self representation. Second, according to the verification results of hypotheses for the influence of parent-adolescent affective bonding on God image, it was found that sense of unity and dedication as variables related to parent-adolescent affective bonding have statistically significant correlation with sense of belonging, sense of approval and sense of control as variables related to God image. Through the study results discussed above, it was verified that the parent-adolescent affective bonding has an effect on the practical aspects of healthy self-representation of the Christian adolescents as well as positive image of God.

적응 신호 처리를 위한 고속 선형 위상 FIR 필터

최승진(Choi Seung-jin),이철희(Lee Chul-heui),양흥석(Yang Heung-suk) 제어로봇시스템학회 1988 제어로봇시스템학회 국내학술대회 논문집 Vol.1 No.1

최적경로 예측을 위한 포인트 클라우드 기반 차선검출

최승진(Seung-Jin Choi),정민구(Min-Gu Jeong),김도훈(Do-Hun Kim),박상현(Sang-Hyun Park) 대한전자공학회 2022 대한전자공학회 학술대회 Vol.2022 No.6

전립선암에서 골전이 진단에 대한 F-18 FDG PET/CT와 골스캔의 불일치

최승진 ( Seung Jin Choi ),김철수 ( Chul Soo Kim ),변성수 ( Sung Su Byun ),현인영 ( In Young Hyun ) 대한핵의학회 2006 핵의학 분자영상 Vol.40 No.5

상행대정맥 종양혈전을 동반한 재발성 간세포암 환자의 F-18 FDG PET/CT소견

최승진 ( Seung Jin Choi ),김철수 ( Chul Soo Kim ),변성수 ( Sung Su Byun ),이경희 ( Kyung Hee Lee ),현인영 ( In Young Hyun ) 대한핵의학회 2006 핵의학 분자영상 Vol.40 No.5