NaNO₃, NaHCO₃ 농도가 Arthrospira platensis 생장에 미치는 영향

최수정,하종명,이재화,Choi, Soo-Jeong,Ha, Jong-Myung,Lee, Jae-Hwa 한국생물공학회 2015 KSBB Journal Vol.30 No.6

Arthrospira platensis (A. platensis) is one of the most explored cyanobacteria and has been studied for proteins, vitamins, pigment (chlorophyll and carotenoids) and fatty acid. In this study, we tested the effect of $NaHCO_3$ and $NaNO_3$ on the microalgae growth under photoautothrophic culture in A. platensis. As a result, cell growth and dry cell weight were increased in proportion to the $NaHCO_3$ and $NaNO_3$ concentration. Pigment (chlorophyll and carotenoids) contents of A. platensis were increased with proportion to $NaHCO_3$ concentration. But, the content of pigment (chlorophyll and carotenoids) in 100% $NaNO_3$ medium of A. platensis was the highest, 40%, 140% and 200% $NaNO_3$ medium with pigment content of A. platensis was reduced. In conditions of $NaHCO_3$ (50%) or $NaNO_3$ (40%) limitation, A. platensis could accumulate lipids to high as 1.7-fold and 1.3-fold.

항산화능이 증대된 Arthrospira platensis 균주 개량 및 특성 분석

최수정(Soo-Jeong Choi),이재화(Jae-Hwa Lee) 한국생물공학회 2015 KSBB Journal Vol.30 No.3

Arthrospira platensis (A. platensis) is a microscopic and filamentous cyanobacterium that derives its name from the spiral or helical nature of its filaments. In this study, we induced mutants of A. platensis through NMU treatment and selected two strains by level of ipid contents. We named mutant ‘1-9’, ‘2-5’, and they were cultivated in the same way with the wild type. During 12 days cultivation, cell growth, dry cell weight, pigment content, and lipid content were measured for characteristics of mutants. As a result, pigment and lipid content of mutants were increased about 3.6, 1.8 times compared with wild type, respectively. It was shown that total flavonoid and polyphenol contents of mutants were increased about 1.5 times compared with wild type. And radical scavenging effect of mutants were increased about 10% compared with wild type.

혈액투석 : 혈액투석 환자에서 초기 동정맥루 혈류량이 동정맥루 생존에 미치는 영향

최수정 ( Soo Jeong Choi ),신동원 ( Dong Won Shin ),김덕수 ( Duk Su Kim ),홍성아 ( Seong Ah Hong ),박무용 ( Moo Yong Park ),김진국 ( Jin Kuk Kim ),황승덕 ( Seung Duk Hwang ) 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1

생활체육 참여 지체장애인의 자기격려가 사회성 향상 및 스포츠자신감에 미치는 영향

최수정 ( Choi Soo-jeong ),이현수 ( Lee Hyun-su ) 한국체육학회 2020 한국체육학회지 Vol.59 No.4

본 연구의 목적은 생활체육에 참여하는 지체장애인의 자기격려가 사회성 향상과 스포츠 자신감에 미치는 영향을 규명하고자 하는데 있다. 본 연구는 부산광역시와 경상도에 거주하고 있는 지체장애인 중 생활체육에 참여하고 있는 263명을 대상으로 연구의 목적에 맞게 수정·보완한 조사도구를 사용하여 설문조사를 실시하였다. 자료처리는 SPSS 25.0 프로그램을 사용하여 탐색적 요인분석과 신뢰도분석, 중다회귀분석을 실시하였다. 그 결과 첫째, 생활체육 참여 지체장애인의 자기격려는 사회성 향상에 유의한 영향을 미치는 것으로 분석되었다. 둘째, 생활체육 참여 지체장애인의 자기격려는 스포츠 자신감에 유의한 영향을 미치는 것으로 분석되었다. 셋째, 지체장애인의 사회성은 스포츠 자신감에 유의한 영향을 미치는 것으로 분석되었다. 이러한 연구결과를 통해 생활체육에 참여하고 있는 지체장애인의 자기격려는 사회성을 향상시키고 스포츠 자신감을 높이는데 긍정적 요소가 되는 것을 확인할 수 있다. 그러므로 지체장애인 스스로 생활체육을 참여하며 자신에 대한 격려와 자신감을 향상 시킬 수 있도록 건강한 생활체육이 지속적으로 이루어져야 할 것이며, 생활체육뿐만 아니라 장애인스포츠 종목별 사회성 및 스포츠자신감의 향상이 이루어질 수 있도록 지속적인 연구가 필요하다고 사료된다. The purpose of this study is to find out the impact of self-encouragement of the disabled who participate in life-time sports on social improvement and sports confidence. This study was conducted on 263 disabled people living in Busan City and Gyeongsangdo Province who participated in life-time sports using revised and supplemented questionnaires to suit the purpose of the study. Data processing was performed using the SPSS 25.0 program to perform exploratory factor analysis, reliability analysis, multiple regression analysis. As a result, it was analyzed that the self-encouragement of the disabled who participated in the life-time sports has a improvement of sociality. Second, it was analyzed that self-encouragement of disabled people participating in life-time sports has a significant impact on sports confidence. Third, it was analyzed that the sociality of the Physical Disabilities has a significant impact on sports confidence. These findings confirm that self-encouragement of disabled people participating in daily sports is a positive factor in improving social skills and improving sports confidence. Therefore, it is believed that healthy daily sports should be continuously conducted so that disabled people can participate in daily sports themselves and improve their sports confidence, and that continuous research is needed to improve sociality and sports confidence in sports for the disabled as well as.

NaNO₃, NaHCO₃ 농도가 Arthrospira platensis 생장에 미치는 영향

최수정(Soo-Jeong Choi),하종명(Jong-Myung Ha),이재화(Jae-Hwa Lee) 한국생물공학회 2015 KSBB Journal Vol.30 No.6

Arthrospira platensis (A. platensis) is one of the most explored cyanobacteria and has been studied for proteins, vitamins, pigment (chlorophyll and carotenoids) and fatty acid. In this study, we tested the effect of NaHCO₃ and NaNO₃ on the microalgae growth under photoautothrophic culture in A. platensis. As a result, cell growth and dry cell weight were increased in proportion to the NaHCO₃ and NaNO₃ concentration. Pigment (chlorophyll and carotenoids) contents of A. platensis were increased with proportion to NaHCO₃ concentration. But, the content of pigment (chlorophyll and carotenoids) in 100% NaNO₃ medium of A. platensis was the highest, 40%, 140% and 200% NaNO₃ medium with pigment content of A. platensis was reduced. In conditions of NaHCO₃ (50%) or NaNO₃ (40%) limitation, A. platensis could accumulate lipids to high as 1.7-fold and 1.3-fold.

지질 고 생산성 Chlorella vulgaris 변이주 분리 및 특성 분석

최수정 ( Soo Jeong Choi ),박현진 ( Hyun Jin Park ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2015 공업화학 Vol.26 No.5

Micro-algae Chlorella vulgaris (C. vulgaris) is an important source for bio-diesel because of the high content of neutral lipids. In this study, we intended to induce mutants of C. vulgaris by UV-B irradiation. C. vulgaris was first exposed to UV-B for 1, 2, 3, 4, 5 min. As the UV-B exposure time increased, the cell viability and pigment content were decreased. Mutants of C. vulgaris were also induced through ultraviolet irradiation and two strains were selected with respect to lipid contents, where were named as ‘UM10’, ‘UM15’. They were then cultivated in the same way as to the wild type. After 21 days of cultivation, the cell growth, dry cell weight, pigment contents, and lipid contents were measured for investigating characteristics of mutants. As a result, the cell growth and dry cell weight of both mutants increased about 1.4 and 1.5 times, respectively compared with those of wild type. In addition, chlorophyll and carotenoid contents were measured in order to investigate pigment contents in micro-algae through photosynthesis. It was shown that chlorophyll and carotenoid contents of both mutants decreased about 10% compared to those of wild type. Lipid contents in UM10 and UM15 increased about 1.2 and 1.5 times, respectively compared to that of wild type.

전자빔 조사를 통해 유도된 Arthrospira platensis 변이주 분리 및 특성 분석

최수정 ( Soo Jeong Choi ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2015 공업화학 Vol.26 No.5

Arthrospira platensis has high lipid and pigment (such as chlorophyll and carotenoid) contents and thus evaluated as an important resource in functional food production. The cell growth rate and pigment concentration of EM24 increased by approximately 1.2-fold than those of the wild-type strain (WT). Fluorescence intensity levels in EM24, which were quantified with a lipid triolein standard curve, also increased by approximately 1.5-fold than those in WT (62.9 mg/Lvs. 38.9 mg/L). The analysis of fatty acid profiles indicated that the gamma-linoleic acid level in EM24 increased by 1.5-fold than that in WT.

혈액투석 환자에게서 Biohole(TM) 쐐기를 이용한 Buttonhole 천자법의 임상적 유용성

최수정 ( Soo Jeong Choi ),이설형 ( Seol Hyoung Lee ),조은희 ( Eun Hee Cho ),오혜란 ( Hye Ran Oh ),김은정 ( Eun Jung Kim ),박무용 ( Moo Yong Park ),김진국 ( Jin Kuk Kim ),황승덕 ( Seung Duk Hwang ) 대한내과학회 2012 대한내과학회지 Vol.82 No.3

Background/Aims: Buttonhole cannulation has been popular because it provides an easy puncture, is less painful, and requires less time for hemostasis. However, the technique requires a skilled staff and a long time to form the tract. A new buttonhole technique using Biohole(TM), which shortens the time needed for tract formation, has been introduced in Europe, North America, and Japan. Methods: We prepared a cannulation tract using the buttonhole technique and Biohole(TM) over a two-week period and compared the 12-week outcomes between patients who underwent the rope-ladder versus buttonhole techniques. Results: The 40 patients (27 males) had a mean age of 49.1±14.2 years. Thirteen and 27 patients were cannulated with the rope-ladder and buttonhole techniques, respectively. Patients who underwent the buttonhole technique had more initial pain than did those who received the rope-ladder technique (p=0.044). The Biohole(TM) procedure improved puncture pain (5.6 vs. 3.4, p=0.003) and shortened hemostasis time (1.8 vs. 1.3, p=0.001). Over a two-week period, patients using Biohole(TM) experienced dislocation (20.8%), bleeding at peg sites (8.6%), and pain during peg change (2.4%). Over the 12 week study period, patients who underwent the buttonhole technique had insignificantly less pain than did those who received the rope-ladder technique (p=0.088), but the former had less bleeding time than the latter (p=0.000). One patient who received the buttonhole technique experienced one episode of infection (p=0.327). Conclusions: The new buttonhole technique using Biohole(TM) is safe and useful in the short term. A long-term, larger, multicenter study is required to confirm these results. (Korean J Med 2012;82:307-312)

전자빔 조사에 의해 지질 함량이 증대된 Arthrospira platensis 변이주 분리 및 지방산 분석

최수정 ( Soo Jeong Choi ),김영화 ( Young Hwa Kim ),김안드레 ( Andre Kim ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2013 공업화학 Vol.24 No.6

Arthrospira platensis (A. platensis) is an economically important microalgae because it has carbohydrates, lipids, proteins and a number of phytochemicals. It is also a valuable source used in the production of biodiesel and functional foods. In this study, A. platensis was exposed to electron beam irradation (240 kGy) and induced random mutagenesis for strain improvement. Several mutants were obtained, and the resulting mutant was designated as EB29. The growth rate and chlorophyll content of EB29 was similar to those of wild type. However, the lipid content of EB29 was increased seven-fold compared to that of wild type when comparing the nile red fluorescent intensity. Semi-quantitative analysis of EB29 using the calibration plot of standard lipid, triolein, represented 78.6 μg/mL, which increased 2 times compared to wild type (41.4 μg/mL). When analyzing the fatty acid profile of EB29, polyunsaturated fatty acids (PUFAs), such as gamma-linolenic acid (GLA) in EB29 increased about six-fold. Moreover, fatty acids affecting the quality of biodiesel increased compared to that of wild type. Thus, electron beam could be used for the strain improvement of microalgae in order to accumulate PUFAs and alteration of fatty acid profile for biodiesel.

나노기포 산소수 및 수소수가 미세조류 배양에 미치는 영향

최수정 ( Soo Jeong Choi ),김영화 ( Young Hwa Kim ),정인하 ( In Ha Jung ),이재화 ( Jae Hwa Lee ) 한국공업화학회 2014 공업화학 Vol.25 No.3

Microalgae Nannochloropsis oculata (N. oculta) and Chlorella vulgaris (C. vulgaris) are important sources for biodisel becauseof the high content of neutral lipids. Stable nano bubble is maintained for a long time and therefore it is possible for usein biotechnology. In this study, effects of nano bubble oxygen or hydrogen water on the microalgae growth were characterized. The cell growth in nano bubble water was similar to that of control, and the total lipid content was rather low. But, chlorophyllcontent of N. oculata grown in nanno bubble oxygen and hydrogen water increased 54% and 30%, and increased 59%, 39%in C. vulgaris. Carotenoid content also increased 21%, 25% in N. oculata and 49%, 29% in C. vulgaris grown in nano bubbleoxygen and hydrogen water. From these results, nano bubble water seems to enhance the photosynthetic capacity ofmicroalgae.