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한국아동(韓國兒童)의 치아우식경험과 치면상(齒面上) Streptococcus mutans 분포(分布)에 대(對)한 연구(硏究)
김각균,최선진,임창윤,장우현,Kim, Kack-Kyun,Choe, Son-Jin,Lim, Chang-Yoon,Chang, Woo-Hyun 대한미생물학회 1983 大韓微生物學會誌 Vol.18 No.1
Various investigations have been carried out to elucidate the causative relationship between specific oral bacterial species and dental caries since it was first demonstrated that selected streptococoal species produced dental caries in germfree rats when fed a high-sucrose diet. Now, S. mutans is considered to play an important role in the development of dental caries in animals and humans, and only a limited number of species of bacteria other than S. mutans are occasionally found to be cariogenic in experimental animals. In this regard, association of the number of S. mutans in approximal plaque with caries experience (DMFT) was studied from 137 Korean school children($10{\sim}11$ year old). Biotypes of the collected strains of S. mutans were determined, and their relationship with caries status was also examined. The following results were emerged from the study. 1. S. mutans was detected in the plaques of all children. 2. Statistically significant positive correlation(r=0.445, p<0.001) was found between the caries experience(DMFT) and the number of S. mutans in approximal plaques. 3. The number of S. mutans were significantly higher(p<0.001) in plaques removed from carious surface than from sound surface. 4. The most frequent biotype was biotype I(78.8%), followed by IV(33.1%) and V(09.5%). Biotype II was isolated in the plaque of two children(1.7%) only. 5. There was no apparent relation of specific biotypes to carious status.
최선진,허준호,김철위 大韓齒科器材學會 1990 대한치과재료학회지 Vol.17 No.1
The aim of the present investigation was assess the thermal reaction during hardening of representative fourteen commercially available conventional dental amalgam alloys: DA HA HV KA AC CA FC IH MM OT SS SCA SA UT and six high-copper dental amalgam alloys: A-21 KS LA OP PM PA used in Korea. These tests were performed with a thermistor probe (YSI Model 42 SC) using physiograph(MK-IV, Narco, Biosystems, U.S.A.). The peak temperature of the thermal reactions of dental amalgams were determined by the mean value of three measurements. From the experiments, the results were as follows: 1. The results indicate that the peak temperature of exothermic reactions of the conventional amalgams was attained during 7.67 ±1.76℃ and 15.50 ±0.50℃, respectively. 2. The results suggest that the exothermic peak temperature for the high-copper amalgams was considerably higher than those of the conventional amalgams. 3. The results for the recommended ratio revealed significant increases in exothermic reactions and the (+) modified ratio showed significantly less exothermic reactions than the (-) modified ratio. 4. The results show that the peak temperature rise of dental amalgams was attained during 7.17 ±0.29℃ and 24.17 ±0.29℃ according to the type of amalgamators, respectively.
최선진,김철위 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2
Based on the report that the adherence of S. mutans 10449 was enhanced by the presence of glucan formed in situ on the salivary pellicle, the present experiment was carried out to elucidate the role that glucans play in the enhancement. It was asked whether the gluans are involved directly, or indrectly by affecting other adhesion-related factors. In addition, the property of the adhesin which responds to the in situ formed gluan was examined. Glucosyltransferase(GTF) was prepared by ammonium sulfate fractionation of S. mutans 6715-7 culture supernatant. Saliva-coated hydroxyapatite beads(SHA) were treated with GTF, then incubated with 5% sucrose to allow in situ glucan synthesis(SHA/glucan). The pellicles or bacterial suspensions were treated with heat, trypsin or periodate. For the bacterial adherence assay, SHA or SHA/glucan was combined in polypropylene microfuge tubes with [^3H]-thymidine labeled S. mutans 10449 and incubated. The beads were then washed and counted with scintillation fluid to determine the number of adherent bacteria. Although the pretreatment of pellicles with heat or trypsin reduced significantly the adhesion of S. mutans 10449 to SHA/glucan, periodate pretreatment caused the most reduction, implying that the glucan is directly involved in the bacterial adhesion. When the bacteria were pretreated with heat or trypsin, the latter influenced the adhesion mostly. This suggests that the bacterial adhesin involved is of protein-nature.
Hydroxyapatite 비드에 부착한 [^3H] - 표지된 세균의 씬틸레이션 측정에 관한 연구
이시영,이장희,최선진 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.2
This study investigated the possible involvement of sample self absorption when [^3H]-labeled Streptococcus mutans cells that adhered to spheroidal hydroxyapatite beads were counted by scintillation spectrophotometer. To determine whether or not the beads cause self absorption of the energy of β particles, the beads-adhered bacterial cells were removed and counted alone. Two procedures were employed to remove bacterial cells from the beads. First, bacteria-adhered beads were dissolved with 0.33 N HCl, the dissolved solution was filtered to collect bacteria, and the filter was counted. Second, the bacteria adhered to beads were desorbed with potassium phosphate buffer, bacteria were collected on filter, and the filter was counted. Whatman glass microfibre filters GF/A and GF/F were used and filtration was carried out either with suction or without suction (i.e., by blotting). When the radioactivity was counted by blotting on GF/F filter, the count rate was increased to 280% in HA and 240% in SHA as compared with CPM of the control. Count rate of the bacteria was lower when filtered with suction than without suction. However, there was no difference in count rate when intact cell suspensions filtered either with suction of without were counted. Though HCl-treated cells were observed as intact by Gram staining, the above results seem to indicate that cells were damaged when treated with HCl, thus some of the DNA leaked out of the cells when filtered with suction. When the buffer-desorbed bacterial cells were filtered by blotting and counted, the count rate was increased to 290% in HA and 350% in SHA as compared with CPM of the control. The results of the present study mean that a high count rate is obtainable when the sample self absorption is removed. Therefore, the sample self absorption should be considered in order to measure the correct number of bacteria adhered to HA beads.
Whole-genome DNA 프로브를 이용한 구강 Bacteroides 균종의 식별
최세원,이정숙,최선진,김각균 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.1
The need for a rapid and sensitive microbiological assay has become necessary for both research and clinical diagnostic. This need has become clear as a result of extensive documentation linking specific bacterial species and periodontal destruction. DNA probe technology seems to be able to provide both a sensitive and specific assay and may be able to alleviate the concern for transport of fastidious microorganisms. We have been trying to establish the method of identifying the periodontopathic microorganisms by DNA probe. As the first step we tried to know if whole-genomic DNA probe could differentiate between bacterial species, using three different serotype-strains of both bacteroides gingivalis and bacteroides intermedius. Also we tested if there is any cross-hybridization between bacteroides spp· and Streptococcus spp. The experimental procedures were as follows: anaerobic culture of bacterial cells, preparation of whole-genomic DNA from bacteria and measurement of DNA concentration, immobilization of DNA onto a nitrocellulose filter using dot-blot apparatus after 2-fold serial dilution of DNA, radio-labelling of DNA with ^32P by random oligonucleotide-primed synthesis, which would serve as a DNA probe, blocking unbound nitrocellulose with 0.05×BLOTTO, hybridization of the filter with ^32P-labelled probe, and washing and detection of bound probe by autoradiography. The results were as follows. The whole-genomic DNA probe of B. gingivalis strain 381 (serotype a) was able to hybridize with all three serotypes a, b, and c) of the species. Also, the whole-genomic DNA probe of B. intermedius strain 9336 (serotype b) was able to hybridize with all three serotypes of the species. There was no detectable cross-hybridization between B. gingivalis and B. intermedius or bacteroides DNA probe with streptococcal DNA, within the range of DNA amount used in the experiment. The DNA probe was able to detect 3ng of homologous DNA in a dot. It seems likely that whole-genomic probe could be used for identification of Bacteroides spp, in the clinical samples using DNA probe, if a suitable method to lyse the cell on the filter is provided.
구강 Streptococci의 적혈구와 타액 유도응집 및 하이드록시아파타이트에의 부착에 관한 연구
송요한,최선진 대한구강생물학회 1986 International Journal of Oral Biology Vol.10 No.2
As a part of a series of investigation on the mechanisma of adherence to tooth and oral soft tissues treptococcus mutans and S. sanguis, the two important microorganisms of oral streptococci, a study was carried out to screen laboratory strains and fresh isolates of both S. mutans and S. sanguis in terms of their adherence to the saliva-coated hydroxyapatite, hemagglutination, and salivary agglutination. The following data were obtained. a. The adherence of S. mutans strains to hydroxyapatite was inhibited by saliva, whereas saliva enhanced the adherence to hydroxyapatite of most of the S. sanguis strains tested. S. sanguis strain 66 49 was shown to have a property to adhere well to hydroxyapatite even in the absence of saliva, whereas the S_1 strain, one of fresh S. sanguis isolates was identified as a bacterium whose adherence was strongly enhanced by saliva. b. Many more strains of S. mutans showed positive hemagglutination reaction compared to the strains of S. sanavis. Among the positive strains, S. mutans M5 and S. sanguis S5 showed the highest hemagglutination titers. c. Salivary agglutination reactions were positive in all of the strains tested, and those strains which showed the highest agglutination titers were S. mutans OMZ175 and M2, S. sanguis MPC1 and 66 49. d. No correlation in 3 reactions was observed among the bacterial strains tested.