치주질환의 면역학

김각균,Kim, Kack-Kyun 대한면역학회 2003 Immune Network Vol.3 No.4

The periodontal diseases are infections caused by bacteria in oral biofilm, a gelatinous mat commonly called dental plaque, which is a complex microbial community that forms and adhere to tooth surfaces. Host immune-pathogen interaction in periodontal disease appears to be a complex process, which is regulated not only by the acquired immunity to deal with ever-growing and -invading microorganisms in periodontal pockets, but also by genetic and/or environmental factors. However, our understanding of the pathogenesis in human periodontal diseases is limited by the lack of specific and sensitive tools or models to study the complex microbial challenges and their interactions with the host's immune system. Recent advances in cellular and molecular biology research have demonstrated the importance of the acquired immune system in fighting the virulent periodontal pathogens and in protecting the host from developing further devastating conditions in periodontal infections. The use of genetic knockout and immunodeficient mouse strains has shown that the acquired immune response, in particular, $CD4^+$ T-cells plays a pivotal role in controlling the ongoing infection, the immune/inflammatory responses, and the subsequent host's tissue destruction.

한국아동(韓國兒童)의 치아우식경험과 치면상(齒面上) Streptococcus mutans 분포(分布)에 대(對)한 연구(硏究)

김각균,최선진,임창윤,장우현,Kim, Kack-Kyun,Choe, Son-Jin,Lim, Chang-Yoon,Chang, Woo-Hyun 대한미생물학회 1983 大韓微生物學會誌 Vol.18 No.1

Various investigations have been carried out to elucidate the causative relationship between specific oral bacterial species and dental caries since it was first demonstrated that selected streptococoal species produced dental caries in germfree rats when fed a high-sucrose diet. Now, S. mutans is considered to play an important role in the development of dental caries in animals and humans, and only a limited number of species of bacteria other than S. mutans are occasionally found to be cariogenic in experimental animals. In this regard, association of the number of S. mutans in approximal plaque with caries experience (DMFT) was studied from 137 Korean school children($10{\sim}11$ year old). Biotypes of the collected strains of S. mutans were determined, and their relationship with caries status was also examined. The following results were emerged from the study. 1. S. mutans was detected in the plaques of all children. 2. Statistically significant positive correlation(r=0.445, p<0.001) was found between the caries experience(DMFT) and the number of S. mutans in approximal plaques. 3. The number of S. mutans were significantly higher(p<0.001) in plaques removed from carious surface than from sound surface. 4. The most frequent biotype was biotype I(78.8%), followed by IV(33.1%) and V(09.5%). Biotype II was isolated in the plaque of two children(1.7%) only. 5. There was no apparent relation of specific biotypes to carious status.

한국여성의 질내 Lactobacillus 균종분포

김각균(Kack Kyun Kim),국윤호(Yoon Hoh Kook),장우현(Woo Hyun Chang) 大韓微生物學會 1981 大韓微生物學會誌 Vol.16 No.1

韓國兒童의 齒牙?蝕經驗 과 齒面上 Streptococcus mutans 分布 에 對한 硏究

김각균(Kack Kyun Kim),최선진(Son Jin Choe),임창윤(Chang Yoon Lim),장우현(Woo Hyun Chang) 大韓微生物學會 1983 大韓微生物學會誌 Vol.18 No.1

한국 치의학 교육 평가인증제도의 현황과 발전방향

김경년(Kyung-Nyun Kim),김윤진(Yun-Jin Kim),김주아(Ju Ah Kim),김지연(Ji-Youn Kim),김각균(Kack-Kyun Kim),이재일(Jae Il Lee),신제원(Je-Won Shin) 대한치과의사협회 2015 대한치과의사협회지 Vol.53 No.3

The Korean Institute of Dental Education and Evaluation (KIDEE) was established in 2007 to provide the quality assurance in dental education. The KIDEE has been recognized by The Ministry of Education from Jan. 2015 for 5 years. KIDEE had accredited basic dental education programs of all 11 Korean dental schools. The dental accreditation system was introduced to encourage the improvement for dental schools, to ensure the quality of dental practice and most of all, to establish an internationally compatible system of evaluation and accreditation. The accreditation system in Korea is supported by voluntary service of many dental professionals and contribute to improve the quality of dental education program in all institutions. The accreditation by KIDEE let the students and the dentists be taught with assured education program and the all Koreans be cared by the qualified dentists. A quality education system thus provides a sufficient number of qualified dental health professionals to meet the workforce needs of the nation. Ultimately, this should result in quality oral health care for the public. Finally, by specifying the competencies of graduates, the standards will define the scope of dental practice. This may serve to define the profession of dentistry and differentiate it from other health professions, or to differentiate among the specialties in dentistry in the case of advanced dental education programs.

치태형성기전과 수종약물의 억제작용에 관한연구

김중수(Joong Soo Kim),김형찬(Hyung Chan Kim),최선진(Sun Jin Choih),김각균(Kack Kyun Kim),이종흔(Jong Heun Lee),정동균(Dong Kyun Cheong) 대한치과의사협회 1983 대한치과의사협회지 Vol.21 No.10

교재중심 강의와 문제중심학습 방식이 기초의학에서 과학적 추론에 미치는 효과 비교

김현아,김각균,이승우,Kim, Hyeon-A,Kim, Kack-Kyun,Lee, Sung-Woo 대한안면통증구강내과학회 2005 Journal of Oral Medicine and Pain Vol.30 No.1

Purpose: The aim of this preliminary study was to evaluate the effect of Problem-Based Learning (PBL) curriculum on development of comprehension of basic medical knowledge and quality of semi-structured problem solving including scientific reasoning skill. This scientific reasoning contained five components including: size of simple, design of research cause-effect, construction of risk factor, analysis statistic of data, interpretation of result. Materials and Methods: Seoul National University Dental students (100) participated in this experience during two weeks, 2004. Forty eight multiple-choice questions (MCQ) concerned "Infection Control and Prevention" were asked before and after two sections of Lecture-Based Learning (LBL) and PBL (pretest-posttest control group design). A semi-structured problem in epidemiological research was asked to these students after two sections (posttest-only control group design). Data (mean and SD) were analysed using the t Test for two independent samples (p<.05), comparing PBL versus LBL. Results: Our analyse of scores show no difference between LBL and PBL in the development of comprehension of "Infection Control and Prevention". The quality problem solving (epidemiological research) was significantly different between the two groups (p=.029); specially, two components' scores of reflection on scientific reasoning cause-effect (p=.000) and interpretation of result (p=.001) were significantly better for PBL than for LBL. Conclusion: Theses results indicate that comparing LBL and PBL, PBL curriculum have not been disadvantaged in comprehension of basic knowledge, and have contributed to develop the scientific reasoning in problem solving.

P.intermedia의 유전자 이종성과 가족내 전이에 관한 연구

이승민,김각균,정종평,Lee, Seoung-Min,Kim, Kack-Kyun,Chung, Chong-Pyoung 대한치주과학회 1995 Journal of Periodontal & Implant Science Vol.25 No.1

P. intermedia are considered an important pathogen in adult periodontitis, rapidly progressing periodontitis, refractory periodontitis, pregnancy gingivitis, acute necrotizing ulcerative gingivitis, pubertal gingivitis. So far 2 DNA homology groups and 3 serotypes of P. intermedia have been reported but there is no data available as yet regarding genetic diversity for the species P. intermedia. The purpose of this study is to investigate, using bacterial DNA restriction endonuclease analysis, genetic diversity between individual strains of P. intermedia which are indistinguishable by serotyping and biotyping, occurrence of an intrafamilial transmission and genetic heterogeneity between P. intermedia strains isolated within a patient and within the same serotypes. The families who have had no systemic disease, no experience of periodontal treatment for the previous 1 year and no experience of antibiotics for the previous 6 months were selected and subgingival plaque was collected at 4 sites in each person and incubated in the anaerobic chamber. P. intermedia were identified by colony shape, gram stain, biochemical test, SK-I03(Sunstar Inc.) test and IIF using monoclonal antibody was perfomed for the determination of serotypes. P. intermedia strains were grown in BHI broth and whole genomic DNA was extracted and digested by restriction endonuclease. The resulting DNA fragments were separated by agarose gel electrophoresis, stained and photographed under UV. As the results of this study, intrafamilial vertial transmissions could be assessed in 2 families and horizintal transmissions in another 2 families. There were different DNA digest patterns within a patient, so P. intermedia showed that individuals could be colonized by multiple clonal types at anyone time. And different serotypes could be found within a patient and in the same serotype within a patient, obvius genetic heterogeneity could not be assessed. But in the same serotype in different famies, there were differences in the DNA digest patterns.

THE EFFECTS OF TETRACYCLINE-CONTAINING GEL ON GINGIVAL CREVICULAR ENZYME ACTIVITY

장미경,한수부,김각균,손성희,심창구,Chang, Mee-Khyoung,Han, Soo-Boo,Kim, Kack-Kyun,Son, Seong-Heui,Shim, Chang-Koo The Korean Academy of Periodontoloy 1994 Journal of Periodontal & Implant Science Vol.24 No.3

본 연구의 목적은 비외과적 치주치료시에 부가적으로 사용하기 위해 개발한 테트라싸이클린 젤의 효과를 임상지수, 치은 열구액양 및 치은열구액내 효소활성도 측정을 통해 평가함에 있다. 치주낭 깊이가 4mm 이상 6mm이하인 구치가 편악당 적어도 2개 이상 존재하는 중등도 치주염에 이환된 환자 23명을 선택하여, 한 편악에서는 치근활택술 만을 시행하였고, 다른 한 편악에는 치근활택술 후 테트라싸이클린 젤을 치은연하로 주입한 뒤 치근면 처리하였다. 사용된 임상검사 지수는 치태지수, 치은지수, 치주낭 깊이, 치은부착 상실도 및 탐침시 출혈로, 각각 치료시작 전과 치료 후 4, 12주에 검사하였으며, 치은열구액내 효소활성도 검사를 위해 치은열구액을 치료시작 전과 치료 후 1, 2, 4, 12주에 각각 채취하고 치은열구액 양을 조사한 뒤 Lactate dehydrogenase(LDH)와 Beta-glucuronidase(BG)의 활성도를 측정하였고, 효소활성도는 LDH는 340nm에서, BG는 550nm에서 각각 흡광도를 조사 분석하여 다음과 같은 결과를 얻었다. 1. 임상지수를 측정 비교한 결과 치근활택술 만으로 치료한 군과 치근활택술 후 테트라싸이클린 젤을 병용한 군 모두에서 임상적으로 치은건강의 현저한 향상을 관찰할 수 있었으나, 통계상 두 군간의 유의성있는 차이는 발견되지 않았다. 2. 치은열구액 양은 치근활택술 후 테트라싸이클린 젤을 병용한 군이 치근활택술만 시행한 군보다 치료 후 2주와 4주에 유의성 있게 감소하였다. 3. LDH와 BG 효소 활성도는 두 군 모두 현저히 감소하였고, 비록 통계학적인 유의성은 관찰할 수 없었으나 치근활택술 후 테트라싸이클린 젤을 병용한 군에서 감소량이 더 크게 나타났다.

보리차(Hordeum vulgare var, hexastichon)가 수산화인회석에 대한 우식유발성 세균의 부착에 미치는 영향

김영재,김종철,김각균,Kim, Young-Jae,Kim, Chong-Chul,Kim, Kack-Kyun 대한소아치과학회 2002 大韓小兒齒科學會誌 Vol.29 No.4

Effect of the roasted barley tea in commercial markets on the adherence to the saliva-coated hydroxyapatite(HA) beads and the cell surface hydrophobicity of Streptococcus mutans and Streptococcus sobrinus as cariogenic microorganism was examined in vitro. Adherence activity and hydrophobicity in bacteria tested in all the barley tea samples decreased and the values were different according to the type of tea and the type of treatment. The inhibition of bacterial adsorption to HA beads suggest that barley tea active molecules as catechins and melanoidins may adsorb to a host surface, preventing the tooth receptor from interacting with any bacterial adhesions. The obtained results showed that the barley tea may inhibit bacterial adherence, the first step of the pathogenesis of dental caries in which these microorganism are involved. 시판되는 보리차 3종(티백형 2종 알곡형 1종)이 우식유발 세균인 Streptococcus mutans와Streptococcus sobrinus가 타액피복 수산화인회석에 부착되는 정도와 이들 세균의 체표소수성에 미치는 영향에 대해 평가하였다. 모든 실험군에서 보리차로 처리하였을 때 수산화인회석에의 부착능과 세균의 체표소수성은 감소하였고 이 값은 보리차의 종류와 처리방법에 따라 상이하였다. 이러한 결과는 보리차에 함유된 활성성분인 catechins와 melanoidins의 영향 때문인 것으로 추측되며 보리차로 전 처리한 수산화인회석에 세균이 부착하는 정도가 감소한 것은 활성물질이 흡착되어 치아표면의 수용체가 세균의 부착소와 작용하는 것을 방해함으로써 치아우식증 병인론의 초기 단계인 부착에 영향을 미칠 수 있다는 것을 의미한다. 본 연구결과 보리차가 우식유발 세균의 주요 독력인자 중 하나인 부착능을 억제하므로 보리차가 이러한 세균이 일으키는 치아우식증을 억제할 수도 있다는 가능성을 보여준다.