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The Microbial Population in the Air of Cultivation Facility of Oyster Mushrooms
천세철,안유나,Sajid Mohamad Khan,정일민,원향연,전창성,박율진 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
The microbial population in the air of mushroom cultivation facility was studied to understand the population structure and size depending on the cultivation methods and regions. The air contents of ten farmers’ oyster mushroom cultivation facilities in Kyunggi province were sampled. The results indicated that there was no difference in population size depending on the regions of mushroom cultivation. In addition, the population size of bacteria in the growth room was bigger than that of the cooling room and outside of the mushroom house, but the fungal population was similar in size between cultivation stages. With regard to population structure, Pseudomonas and Penicillium species were most frequently isolated from the air of oyster mushroom cultivation facility.
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산업안전보건법에 대한 이해가 법 준수 인식에 미치는 영향 - 법에 대한 정서의 매개효과를 중심으로 -
천세철 사단법인 한국안전문화학회 2024 안전문화연구 Vol.0 No.32
본 연구는 건설근로자의 산업안전보건법에 대한 이해, 법에 대한 정서, 법 준수에 대한 인식현황을 분석하고 건설업근로자의 산업안전보건법에 대한 이해와 법에 대한 정서, 그리고 법 준수에 대한 인식과의 관계를 살펴봄으로써 건설업에서의 산업안전보건법의 실효성을 높이는 방안을 모색하고자 하였다. 연구대상은 건설업 안전보건교육에 참가한 건설업근로자 345명으로 선정하였고, 설문응답이 불성실하거나 타당성이 의심되는 23부를 제외한 322명의 자료를 통계분석에 사용하였다. 연구도구는 인구사회학적 문항, 산업안전보건법에 대한 이해에 관한 사항, 산업안전보건법에 대한 정서(느낌)에 관한 사항, 산업안전보건법 준수에 대한 인식에 관한 사항으로 구성되었다. 연구의 분석은 SPSS WIN 29.0 프로그램을 사용하였고, 인구사회학적 특성은 빈도분석을 실시하였으며 척도별 기술통계분석을 실시하였다. 산업안전보건법에 대한 이해와 산업안전보건법에 대한 정서, 산업안전보건법 준수에 대한 인식간의 관련성을 알아보기 위하여 pearson 상관계수를 구하였으며, 산업안전보건법 준수에 관한 법 이해와 정서의 영향을 알아보기 위하여 다중회귀분석을 실시하였다. 또한 산업안전보건법 이해와 법 준수 인식의 관계에서 법에 대한 정서의 매개효과를 알아보기 위하여 Process Macro를 활용하여 매개효과분석을 실시하였다. 본 연구의 결과 첫째, 산업안전보건법에 대한 이해와 법 준수에 대한 인식, 그리고 법에 대한 정서간에는 유의한 상관관계가 나타났다. 둘째, 산업안전보건법에 대한 이해와 법에 대한 정서는 법 준수에 대한 인식에 유의한 영향을 나타내었다. 셋째, 산업안전보건법에 대한 정서는 법에 대한 이해와 법 준수에 대한 인식을 매개하는 역할을 하는 것으로 나타났다. 본 연구는 향후 건설산업에서의 산업안전보건법 이해와 준수에 대한 실태를 파악하고 이를 바탕으로 효과적인 안전보건관리체계를 구축하는 데 기여할 것이다. By examining the relationship between construction workers' understanding of the Occupational Safety and Health Act, their sentiments on the law, and their perception of compliance with the law, and their perception of the law, this study attempted to find ways to increase the effectiveness of the Occupational Safety and Health Act in the construction industry. The subjects of this study were 345 construction workers who participated in safety and health education in the construction industry, and data from 322 people were used for statistical analysis, excluding 23 copies whose survey responses were unfaithful or suspected of validity. The research tools consisted of sociodemographic questions, matters related to understanding the Occupational Safety and Health Act, matters related to emotions (feeling) about the Occupational Safety and Health Act, and matters related to awareness of compliance with the Occupational Safety and Health Act. The SPSS WIN 29.0 program was used for the analysis of the study, and frequency analysis of demographic characteristics was conducted, and descriptive statistics analysis by scale was conducted. The Pearson correlation coefficient was obtained to find out the relationship between the understanding of the Occupational Safety and Health Act, the emotions on Occupational Safety and Health Act, and the perception of compliance with the Occupational Safety and Health Act, and multiple regression analysis was conducted to find out the effect of law understanding and emotion on compliance with the Occupational Safety and Health Act. In addition, a mediating effect analysis was conducted using SPSS Process Macro to find out the mediating effect of emotions on the law in the relationship between the understanding of the Occupational Safety and Health Act and the perception of law compliance. As a result of this study, first, there was a significant correlation between the understanding of the Occupational Safety and Health Act, the perception of law compliance, and the emotions on the law. Second, the understanding of the Occupational Safety and Health Act and the emotions on the law showed a significant effect on the perception of law compliance. Third, it was found that the emotions on the Occupational Safety and Health Act played a role in mediating the understanding of the law and the perception of compliance with the law. This study will contribute to understanding and complying with the Industrial Safety and Health Act in the construction industry in the future and establishing an effective safety and health management system based on this.
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Se-Chul Chun(천세철),Mung Hwa Yoo(류명화),Young Sook Moon(문영숙),Mi Ho Shin(신미호),Ki-Cheol Son(손기철),Ill-Min Chung(정일민),Stanley J. Kays 한국원예학회 2010 원예과학기술지 Vol.28 No.3
Total bacterial populations were cultured from the Hydroball cultivation media in the rhizospheres of 9 different plants including Hedera helix L. and Dracaena deremensis cv. Warneckii Compacta, etc. These cultured bacterial populations were studied to test if the bacterial populations in the plant growing pots may play a role on removal of volatile organic compounds (VOCs) such as benzene and toluene in the air. To meet this objective, first, we tested the possibility of removal of VOCs by the cultured total bacteria alone. The residual rates of benzene by the inoculation of total bacterial populations from the different plant growth media were significantly different, ranging from 0.741-1.000 of Spathiphyllum wallisii ‘Regal’, Pachira aquatica, Ficus elastica, Dieffenbachia sp. ‘Marrianne’ Hort., Chamaedorea elegans, compared to the control with residual rate of 0.596 (LSD, P=0.05). This trend was also similar with toluene, depending on different plants. Based on these results, we inoculated the bacterial population cultured from P. aquatica into the plant-growing pots of P. aquatica, F. elastica, and S. podophyllum inside the chamber followed by the VOCs injection. The inoculated bacteria had significant effect on the removal of benzene and toluene, compared to the removal efficacy by the plants without inoculation, indicating that microbes in the rhizosphere could play a significant role on the removal of VOCs along with plants.
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Chun, Se-Chul,Lee, Keumhee,Song, Y. S.,Ko, K. I.,Kim, B. K. 建國大學校附設農業資源開發硏究所 2002 農資源開發論集 Vol.24 No.-
박과 종자로부터 효율적인 오이녹반모자이크 바이러스(CGMMV) 추출방법을 ELISA에 의하여 조사 비교하였다. 종자를 30분에서 48시간 바이러스 추출 완충 용액에 침지하여 추출한 방법, 2시간 침지 후 2시간 냉동시키고 해동하여 10시간 동안 방치하여 추출한 방법을, 종자를 분쇄하여 추출하는 방법과 비교 평가하였다. 14시간 침지에 의한 추출과, 2시간 침지 후 2시간 냉동, 10 해동 방치 추출한 것은 분쇄법에 의한 추출법과 ELISA의 CGMMV 검출 효율이 유사하였다. 따라서, 노동이 많이 소요되는 분쇄법 대신에 14시간 침지 누출이나, 2시간 침지 후 2시간 냉동, 10시간 해동 방치하는 추출법을 사용할 수 있을 것임을 제시하여 주었다. 또한, 마이크로플레이트의 한 well 당 종자의 양을 1개보다는 10, 20개 사용하는 것이 검출효율이 유사하였다. 이는 총 사용되는 종자의 양이 일정하다고 할지라도, well 당 사용되는 종자가 20개일 경우까지는 well 당 사용되는 종자를 수를 증가시키더라도 바이러스를 검출할 효율은 동일함을 제시하여 주었다. Efficient methods of virus extraction from gourd seeds for the detection of enzyme-linked immnosorbent assay (ELISA) were compared. Seeds were soaked in sample extraction buffer (Agdia, Inc., Elkhart, IN USA) for 30min∼48hrs to exude cucumber green mottle mosaic virus. In addition, 2 hr soaking in sample extraction buffer and 2 hr of freezing followed by 10 hr thawing extract (2S-2F-10T) was used for the detection of the virus by ELISA. Whole seed and seed coat only were extracted for the detection of cucumber green mottle mosaic virus (CGMMV) by ELISA. Also, the effect of amount of seeds on the rate of detection of the virus by ELISA was studied. Soaking seeds for 14hr and 2S-2F-10T methods were similar to seed and only seed coat crushing methods on the efficiency of virus detection, indicating that seed soaking methods for the extraction could be used instead of mechanical breaking seeds with similar detection rate of the virus from gourd seeds. The results suggested that the chance of detection for the virus by increasing amount of seeds up to 20 seeds per well culd be same to one seed per well, although total amount of seeds for detection of the virus would be same for one seed and 20 seeds per well.
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