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장갑열,원향연,최선규,권순익,김규현,공원식,유영복,성재모 한국버섯학회 2007 한국버섯학회지 Vol.5 No.1
Application of sludge wastes into the field may help soil fertility with physical, chemical and biological properties. Efficient use of sludge waste, however, requires an individual assessment of the waste products. A lot of experiment into the organic waste-treated soils has been done for decade. However, studies have not been carried out on the assessment of agricultural soil by Basidiomycota. This study was assessed the influence of sludge application on soil healthy in agricultural upland soils. The organic wastes selected for long-term application experiment in this study were municipal sewage sludge (MSS), industrial sewage sludge (ISS), leather processing sludge (LS), alcohol fermentation processing sludge (FS), and pig manure compost (PMC). To develop the soil healthy assesment method, soil samples were diluted by 20X with distilled water. After shaking at 200rpm for 30 minutes, the shaked sample was mixed on PDA(Potato Dextrose Agar). And sterilized at 121℃ for 20 minutes. Coriolus hirsutus (MKACC 50560) was inoculated on petri-dish including PDA mixed sample. After the media was incubated at 25℃ for five days, the mycelial growth of C. hirsutus was measured. When the mycelial growth on sample media was compared with growth on media contained PDA only, well grown media contained sample soil was assesed as healthy soil. The results suggest that the simple method by Coriolus hirsutus is a handy way to assess the healthy of waste sludge-applied upland soils.
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Impacts of Soil Texture on Microbial Community of Orchard Soils in Gyeongnam Province
김민근,손연균,원향연,허재영,정정석,최용조,이상대,신현율,옥용식,이영한 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.2
Soil management for orchard depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 orchard (5 sites for sandy loam, 7 sites for silt loam, and 13 sites for loam) in Gyeongnam Province by fatty acid methyl ester (FAME) method. The average values for 25 orchard soil samples were 270 nmol g-1 of total FAMEs, 72 nmol g-1 of total bacteria, 34 nmol g-1 of Gram-negative bacteria, 34 nmol g-1 of Gram-positive bacteria, 6 nmol g-1 of actinomycetes, 49 nmol g-1 of fungi, and 7 nmol g-1 of arbuscular mycorrhizal fungi. In addition, silt loam soils had significantly low ratio of cy17:0 to 16:1ω7c and cy19:0 to 18:1ω7c compared with those of loam soils (p < 0.05), indicating that microbial activity increased. The average soil microbial communities in the orchard soils were 26.7% of bacteria, 17.9% of fungi, 12.6% of Gram-negative bacteria, 12.5% of Gram-positive bacteria, 2.5% of arbuscular mycorrhizal fungi, and 2.2% of actinomycetes. The soil microbial community of Gram-negative bacteria in silt loam soils was significantly higher than those of sandy loam and loam soils (p < 0.05).
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정종천,전창성,김승환,원향연,권재건 한국버섯학회 2005 한국버섯학회지 Vol.3 No.4
This study was conducted to verify the cause of suppression symptom in mycelial growth during summer and to be able to establish a countermeasure. Cultivation of Flammulina velutipes was experimented with varying elapsed time of 0, 3, 6, 9 hours after mixing the sawdust media and two kinds of water temperature (normal water, 24℃ and cold water, 6℃) for mixing sawdust media. There were trends of increased media temperature from 24℃ to 31℃ and decreased pH from 6.5 to 5.2∼5.6 with varying elapsed time from mixing the media to sterilization. Bacterial density also increased with bacterial density in Medium 24℃ being 1.9∼4.1 times higher than that in Medium 6. Growth of F. velutipes was delayed with dual culture of bacteria isolated from sawdust media. Total nitrogen content of sawdust media was lowered by elapsed time from mixing the media to sterilization. The use of normal water(24℃) delayed mushroom growth by 1∼2 days compared with that of cold water(6℃). Furthermore, mycelial growth of F. velutipes in the bottle cultivation ceased 9 hours after mixing the media. Primordia formation of F. velutipes was delayed by 1∼3 days by elapsed time after mixing sawdust media, while fruit-body yield decreased by 7∼12% 6 hours after mixing the media. Fruit-body yield was more stabilized with the use of cold water(6℃) than with that of normal water(24℃). Results showed that it is effective to use cold water as low as 6℃ in mixing media for F. velutipes cultivation, especially during summer when environmental temperature is high, high pressure sterilization after bottling work can prevent bacterial propagation in the media and can stabilize media ingredient.
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The Microbial Population in the Air of Cultivation Facility of Oyster Mushrooms
천세철,안유나,Sajid Mohamad Khan,정일민,원향연,전창성,박율진 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
The microbial population in the air of mushroom cultivation facility was studied to understand the population structure and size depending on the cultivation methods and regions. The air contents of ten farmers’ oyster mushroom cultivation facilities in Kyunggi province were sampled. The results indicated that there was no difference in population size depending on the regions of mushroom cultivation. In addition, the population size of bacteria in the growth room was bigger than that of the cooling room and outside of the mushroom house, but the fungal population was similar in size between cultivation stages. With regard to population structure, Pseudomonas and Penicillium species were most frequently isolated from the air of oyster mushroom cultivation facility.
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