Screening of Extracts from Red Algae in Jeju for Potentials MarineAngiotensin - I Converting Enzyme (ACE) Inhibitory Activity

차선희,이기완,전유진,Cha, Seon-Heui,Lee, Ki-Wan,Jeon, You-Jin The Korean Society of Phycology 2006 ALGAE Vol.21 No.3

This study was conducted to screen in vitro angiotensin - I converting enzyme (ACE) inhibitory activities of methanol (MeOH) and aqueous extracts at 20°C and 70°C, respectively, prepared from twenty-six red algae obtained from the coast of Jeju Island in Korea. Among aqueous extracts at 20°C (20AE) from red algae Lomentaria catenata showed the strongest ACE inhibitory activity and Lithophyllum okamurae recorded the second highest activity. From MeOH extract at 20°C (20ME) Ahnfeltiopsis flabelliformis possessed the strongest ACE inhibitory activity. Remarkable activities from MeOH extracts at 70°C (70ME) were observed in Grateloupia filicina, Sinkoraena lancifolia and Grateloupia lanceolata. However, no significant activity was found in aqueous extracts at 70°C (70AE). The IC50 values, which are concentrations required to inhibit 50% activity of ACE, for ACE inhibitory activities of 20AE from Lithophyllum okamurae and L. catenata were 13.78 and 12.21 μg mL–1, respectively. The IC50 values of 20ME from A. flabelliformis and Laurencia okamurae were 13.84 and 106.15 μg mL–1. Those of the 70ME from Bonnemaisonia hamifera, Grateloupia filicina, Sinkoraena lancifolia, G. lanceolata, Gracilaria vermiculophylla and L. okamurae ranged from 25.82 to 124.69 μg mL–1.

Electron Spin Resonance을 이용한 제주 자생 해조류의 DPPH Free Radical 소거활성 검색

차선희,허수진,전유진,Cha, Seon-Heui,Heo, Soo-Jin,Jeon, You-Jin 한국해양바이오학회 2006 한국해양바이오학회지 Vol.1 No.4

본 실험은 electron spin/paramagnetic resonance(ESR/EPR)를 이용하여 기존의 DPPH free radical 소거 활성을 측정하였고, 이를 해조류 유래 천연자원으로부터 새로운 항산화물질을 탐색하는데 적용해 보았다. 연구 결과, 녹조류 추출물에서는 참홑파래, 납작파래 및 청각의 고온 메탄올 추출물에서만 약간의 라디칼 소거활성을 나타내었고, 이 외의 추출물에서는 소거활성이 거의 보이지 않았다. 갈조류는 총 71개의 추출물 중 37개의 추출물에서 80% 이상의 소거율을 보여 아주 우수한 항산화 물질을 가진 family로 여겨지며, 특히 모자반류 (Sargassum spp.)는 수용성과 메탄올, 고온과 상온의 모든 추출물에서 높은 라디칼 소거 활성을 보여 앞으로의 신소재 물질 후보로 선별되었다. 홍조류에서는 수용성보다는 메탄올 추출물에서 대체적으로 높은 소거활성이 검색되어, 나중에는 유기용매 분획물을 제조하여 라디칼 소거능을 측정 할 수 있을 것이다. 이처럼 해조류는 라키칼 소거 능력을 가지는 성분을 여러 형태로 다량으로 함유 하고 있는 것으로 사료 되며 앞으로의 연구에 기대가치를 더해 줄 것으로 사료된다. Extracts which were prepared by four different extractions - 80% methanol extracts (ME) at high ($70^{\circ}C$) and a room temperature ($20^{\circ}C$), respectively and aqueous extracts (AE) at both temperatures with the residue after the methanol extracts - of 10 green, 19 brown and 25 red seaweeds collected in Jeju Island coast were examined for their DPPH free radical scavenging activity using a ESR (electron spin resonance) spectroscopy. A variety of the extracts showed positive scavenging effect against DPPH free radical (except the green seaweeds). Among the extracts, the brown seaweed extracts exhibited the highest scavenging activity. Especially, Sargassum spp. of the brown seaweeds have remarkable scavenging activities - both methanolic and aqueous at the both temperatures ($20^{\circ}C$ and $70^{\circ}C$). On the other hand, ME showed better scavenging activity than AE in the red seaweed extracts. These results indicate that autogenous seaweeds in Jeju will be potential natural antioxidants for functional food compounds.

현미경을 이용한 난관복원술의 임상적 고찰

차선희(SH Cha),이우석(WS Lee),박종영(JY Park),윤태기(TK Yoon),차광열(KY Cha) 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.2

During 2 year period from Jun. 1989 to Dec. 1990, 265 cases of microsurgical tubal reversal were performed and 180 cases of them were followed up for more than 1 year postoperatively in the Department of Obstetrics and Gynecology, Cha General Hospital. The results were as follows : 1. The reasons for requesting reversal of sterilization were a loss of children (25.6%), remarriage (28.9%), change of attitude (45.5%). 2. The mean age was 32.2 years and 91.7% of them had been sterilized by laparoscopic cauterization . 3. The most common site of the anastomosis was isthmic-ampullar portion (65.5%). 4. The postoperative tubal length was 4.1cm or more in 77.2%. 5. No statistical differences were found in pregnancy rate according to the method of previous sterilization, the site of tubal anastomosis and postoperative tubal length. 6. The tubal length did not influence the mean interval form operation to pregnancy. 7. The overall pregnancy rate after tubal reveral was 83.3% and the outcome of the pregnancy was as follows ; term pregnancy 83.6%, premature delivery 0.7%, IUFD 1.3%, spontaneous abortin 9.2%, ectopic pregnancy 3.9%.

40세이상의 여성에서 난관복원술 후 임신율에 관한 임상적 고찰

차선희(SH Cha) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.2

During the 5 year period from Jan. 1989 to Apr. 1994, the women who underwent reversal of sterilization at an age of > 40 years were analyzed. During the study period, a total of women > age 40 underwent reversal of sterilization and 43 cases of them were followed up for more than 1 year postoperatively in the department of obstetrics and gynecology, CHA General Hospital. The results are as follows: 1. The mean age was 41.1 and these 43 procedures amounted to 5.6% of the total number of sterilization reversals(n=764) during the same period in the CHA General Hospital. 2. The mean duration between the operation and the occurrence of the first pregnancy was 7.9 months(range 1 to 19 months). 3. The overall pregnancy rate after tubal reversal was 58.1% and the outcome of the pregnancy was as follows: term pregnancy 46.5%, spontaneous abortion 28%, and ectopic pregnancy 2.3%. 4. According to the method of preceding sterilization, a distinct difference of pregnancy rates in favor of ring sterilization was observed: a 73.3% intraouterine pregnancy rate was achieved after this procedure.

FFT와 그람 - 슈미트 정규직교화를 이용한 워터마킹

차선희(Sunhee Cha),윤희주(Heejoo Yoon),차의영(Euiyoung Cha) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1

분 논문에서는 워터마크가 삽입된 영상의 비가시성과 강인성을 보장하기 위하여 주파수 영역 기반인 FFT(Fast Fourier Transform)을 이용하였다. 그리고 영상에 삽입된 워터마크를 정확하게 추출하기 위하여 워터마크에 삽입하는 키 사이의 직교성을 유지할 수 있는 그람-슈미트 정규직교화를 이용하였다. 실험을 통해 살펴본 결과 영상의 특징에 관계없이 랜덤계열에 민감한 워터마크를 추출할 수 있는 정확성 및 신뢰성을 가짐을 알 수 있었다.

월경전증후군 환자에 있어 임상적 특징에 따른 치료효과

차선희(SH Cha) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.2

The premenstrual syndrome refers to physical or affective synmotoms that appear during the latter half of the menstural cycle, remit during menses, and affect the woman`s relationships or ability oto function. Earlier retrospective histories are considered to be unreliable. Because the criteria used to make the diagnosis varied among groups. Some treatment aim to correct the purported biologic problem underlying premenstrual syndrome, but in the absence of firm knowledge of the cause, specific corrective treatment is not possible. Therefore, much of current therapy has a symptom specific basis. This study was undertaken for the effect of treatment of 44 women with premenstrual syndrome who visited at PMS clinic of Kang Nam St.Mery`s hospital from Oct.1, 1994 to July 31, 1995. The result were as follow: 1. The mean age of patients were 38.65 years old and mean symptomatic period was 8.29 years, and percentage of previous pregnancy complication was 29.3%(Table 1). 2. In residual rate of emotional discomfort, after 3 months was 0.63 compared with initial visit, after 6 month respectively(p

세종음악경연대회 피아노 부문 지정곡의 창작 기법을 응용한 전래동요 및 민요 반주법 모색

차선희(Sunhee Cha),유승지(Seung-Ji Ryu) 한국영유아음악교육학회 2023 영유아음악교육연구 Vol.1 No.2

본 연구는 세종음악경연대회의 피아노 부문 지정곡 중 전통 음계의 구성음을 기초로 반주가 창작된 5곡을 분석한 후, 그 작품들에서 사용된 특징적 반주법을 응용하여 전래동요 및 민요의 분위기를 살릴 수 있는 쉬운 피아노 반주법을 제시하는 데 목적이 있다. 5곡의 분석 결과 작곡가들은 전통 화성 체계에서 주로 사용되는 3도 음정을 쌓아 올리는 형태의 반주를 지양하고, 4도 및 5도 음정 등을 사용하여 서양음악과 구별된 음색을 만들어 낸 것이 발견되었다. 또한 장단의 특징을 효과적으로 보여 주는 단순한 리듬, 셈여림, 아티큘레이션, 및 지시어 등을 사용하여 노래 고유의 느낌을 잘 살려낸 것도 관찰되었다. 이러한 분석 결과를 토대로 연구자는 유선율 피아노 반주 유형 6가지, 무선율 피아노 반주 유형 2가지를 단계적으로 제시하였다. 본 연구를 통해 한국적 분위기를 살릴 수 있는 피아노 반주법의 원리를 이해할 수 있는 계기가 마련되고, 실제 영유아 음악교육 현장에서도 이러한 반주법이 잘 활용되기를 기대한다. The purpose of this study is to analyze five piano works in which the accompaniment was created based on the constituent notes of the traditional scale among the designated works of the Sejong Music Competition and to present an easy piano accompaniment method that can revive the atmosphere of traditional children's songs and folk songs. As a result of the analysis of the five piano works, it was found that the composers avoided stacking 3rd intervals used in the traditional harmony system and used 4th and 5th intervals to create tone colors distinct from Western music. In addition, it was observed that the unique feeling of the Korean traditional song was well preserved by using simple rhythms, dynamics, articulations, and descriptive terms that can showcase the characteristics of the rhythm (Jang-dan). Based on these analysis results, the researcher suggests six types of piano accompaniments that can be used for left-hand accompaniment and two types of piano accompaniments that can be used for both-handed accompaniment with varying levels of difficulty. Through this study, it is expected that an opportunity to understand the principle of the piano accompaniment methods that can revive the Korean atmosphere will be provided, and that such methods will be well used in the field of early childhood music education.

영유아 교육기관의 CCTV 설치에 대한 교사의 경험

차선희(Cha, Seon-Hui),이진숙(Lee, Jin-Sook) 비판과 대안을 위한 사회복지학회 2016 비판사회정책 Vol.- No.51

최근 영유아 교육기관에서 교사에 의한 아동학대가 심각한 사회문제로 부상되면서 CCTV 설치의 의무화가 이루어졌다. CCTV의 설치는 서비스 이용자의 측면에서는 서비스제공 상황을 확인할 수 있어 긍정적이지만, 서비스 제공자인 교사의 측면에서는 인권 침해의 소지를 비롯한 부정적 측면 때문에 상반적으로 평가될 수 있다. 서비스의 질에 대한 영향요인 중 서비스 제공자의 요인이 무척 중요한 상황에서 CCTV의 설치를 교사들이 어떻게 경험하는가 하는 것은 매우 중요한 문제이다. 따라서 본 연구는 CCTV 설치에 대한 교사의 경험을 알아보고자 한다. 이를 위해 영유아교육기관에서의 교사 10명을 연구 참여자로 하여 심층면담을 하여 질적 분석을 수행하였다. 연구결과, CCTV에 대한 교사들의 경험은 긍정적인 측면과 부정적인 측면이 모두 나타났다. 또한 CCTV가 영유아와 교사의 상호작용에 미치는 영향에 대한 인식은 긍정적인 측면으로는 영유아의 관찰을 통해 상호작용을 강화 할 수 있으며, 부정적인 태도로는 교사와 아동 간에 거리감이 증대하고 유대감의 약화, 부자연스러운 행동, 가식적인 행동이 나타날 수 있음이 언급되었다. 그리고 CCTV설치 의무화에 대한 영유아 교사들의 대안에 대한 인식으로는, 먼저 인식개선 방법으로는 사회적 인식개선, 학부모의 인식개선, 교사보호 법안, 교사 처우개선, 사회적 정책 지원 방안의 필요성이 제시되었으며, 효과적인 이용방안 및 대안으로는 열악한 근무개선, 교사의 신뢰, 개방형 기관 만들기, 학부모 보조교사, 보조교사, 부모와 교사의 신뢰가 언급되었다. Recently, as a serious social problem of child abuse by a teacher in kindergarten and daycare centers have been made mandatory installation of cctv. Installation of CCTV is positive because in terms of the service users can check the service status. But in terms of service providers can be assessed opposed from the negative aspects. It is very important for improvement of child services that is known what teachers have been experienced in class room after the installation of CCTV. The purpose of this study was to investigate the experiences of teachers in the space with CCTV. For this purpose, a qualitative analysis was performed by in-depth interviews with 10 teachers in Kindergarten and day care center. As results, the experience of teachers showed both positive side and negative side. After the installation of CCTV, the interaction of the infants and the teachers were influenced both positive and negative as well. Therefore, some alternatives were suggested for improving child services.

양어 EP(Extruded Pellet) 사료 개선용 기능성 천연 다당류 코팅제 개발 및 양식 넙치 사육에 미치는 영향

차선희 ( Seon Heui Cha ),전유진 ( You Jin Jeon ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.1

자궁강내 병변진단에 있어서 자궁난관조영술 , 자궁경검사 , 경질초음파 자궁조영술의 진단적 정확성에 대한 비교 연구

차선희(SH Cha),최정교(JK Choi),이유미(YM Lee),이위현(WH Lee),차경섭(KS Cha) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.8

Hysterosalpingography and Hysteroscopy have been used for the detection of intraute-rine pathology such as polyps, submucous myomas, intrauterine adhesion and endometrial hyperplasia or cancer. Recently the ultrasound has also been utilized for the detection of uterine pathology. Therefore the purpose of this study was to evaluate the diagnostic efficacy of SonoHysterography in detection of intrauterine pathology compared with HSG and Hysteroscopy. 32 patients underwent Sono-Hysterography and Hysteroscopy for the evaluation of the uterine pathology from september 1995 to January 1996. Nine of 32 patients had infertility problem and HSG performed prior to Sono-Hysterography and Hysteroscopy. The results are as follows: 1. The patients` ages ranged from 20 to 50 years(median 37.9). 2. All 9 patients with infertility who had positive HSG findings in uterine cavity showed the intrauterine pathology in Sono-Hysterography as well as Hysteroscopy. The detail findings are as follows:septated uterus(n=2), intrauterine adhesion:IUA(n=3), endometrial polyp(n=3), and IUA combined endmetrial hyperplasia(n=1). 3. Twenty two of 23 patients with abnormal uterine bleeding showed the intrauterne pathology and one patient had negative finding in Sono-Hysterography. However, Hysteroscopy revealed positive intrauterine pathology in 22 patients who had abnormal uterine bleeding. One patients who had positive finding in Sono-Hysterograply showed negative by Hysterography. In contrast, one patient who had negative finding in Sono-Hysterography had positive uterine pathology with polyp in Hysteroscopy. The histologic pathology in all 23 patients reported endometrial polyp(n=12), placental polyp(n=2), submucous myoma(n=1), endometrial hyperplasia(n=5), endometrial cancer(n=1), normal endometrial finding(n=2). 4. Sono-Hysterography, therefore, has a sensitivity and positive predictive value of 96.6%, 93.5% respectively. Our study showed a positive Sono-Hysterography is very predictive of the intrauterine pathology. Sono-Hysterography is safe, quick and minimal invasive procedure. So it is an invaluable technique in the evaluation of uterine cavity.