고압, 효소 처리를 이용한 흑마늘의 제조 및 항산화능 비교검증

조형택,박희규,김영준,육진수 한국식품영양학회 2009 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2009 No.12

Effects of Panax ginseng extracts prepared at different steaming times on thermogenesis in rats

조형택,김준호,이진협,김영준 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3

Background: Panax ginseng (PG) has a long history of use in Asian medicine because of its multiple pharmacological activities. It has been considered that PG in a type of white ginseng may induce undesirable thermogenic effects, but not in a type of red ginseng. However, there is a lack of evidence about the correlation between ginsenoside and thermogenesis. Methods: We investigated the effects of PG with different ginsenoside compositions on body temperature, blood pressure, and thermogenesis-related factors in rats. Results: With increasing steaming time (0 h, 3 h, 6 h, and 9 h), the production of protopanaxadiol ginsenosides increased, whereas protopanaxatriol ginsenosides decreased in white ginseng. In both shortand long-term studies, administration of four ginseng extracts prepared at different steaming times did not induce significant changes in body temperature (skin, tail, and rectum) and blood pressure of rats compared to saline control. In addition, there were no significant differences in the molecular markers related to thermogenesis (p > 0.05), mRNA expressions of peroxisome proliferator-activated receptorgamma coactivator-1a and uncoupling protein 1 in brown adipose tissue, as well as the serum levels of interleukin-6, inducible nitric oxide synthase, and nitrite among the treatment groups. Conclusion: These observations indicate that the potential undesirable effects of PG on body temperature could not be explained by the difference in ginsenoside composition.

The Regulation of Root Hair-specific Expansin Genes

조형택 한국식물병리학회 2004 Plant Pathology Journal Vol.20 No.1

The root hair provides a major entering spot for the symbiotic legume rhizobia. It is obvious that dynamic cell wall modification occurs in the plant root hair during the early microbe invasion. Expansins are nondestructive cell wall-modifying proteins that are involved in cell growth and differentiation. Among about 40 expansin genes in Arabidopsis, two expansin genes are expressed specifically in the root hair cell. Orthologous genes of this Arabidopsis root hair expansins have been found in other Brassica members, rice, and Medicago truncatula (a legume). In this review, I discuss the probable function of expansins during the early symbiotic process between the root hair and microbes and the regulation of root hair expansin genes in a comparative approach.

Explosively Puffed Ginseng Ameliorates Ionizing Radiation-Induced Injury of Colon by Decreasing Oxidative Stress-Related Apoptotic Cell Execution in Mice

조형택,김준호,허완,이현순,이정준,박태식,이진협,김영준 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.5

Although radiation therapy (RT) is a feasible treatment approach for early colorectal cancer, RT is considerably toxic to normal tissues due to the increased reactive oxygen species production, which can induce tissue damage. Ginseng, a natural antioxidant agent, exhibits the protective effects against ionizing radiation (IR)-induced damage in in vitro and in vivo models. The explosive puffing of ginseng has been investigated as a process to improve the efficacy of ginseng due to the resulting physicochemical changes in its functional components. In this study, we provided the evidence for promotion in the beneficial role of puffed ginseng extract (PGE) and associated mechanisms of action, in comparison with white ginseng extract (WGE), against IR-induced colorectal injury, using in vivo study on a mouse model. To study the role of PGE in preventing IR-induced damage, we examined colorectal injury and apoptotic changes in mice exposed to 137Cs at 8 Gy. High-performance liquid chromatography analysis showed that PGE had an increased total ginsenoside concentration with new generation of Rg3, Rg5, and Rk1, compared with the concentrations in WGE. Administering PGE, but not WGE, significantly ameliorated IR-induced colorectal cell death through negative regulation of apoptotic signaling pathways. These antiapoptotic effects of PGE were linked to the capacity to suppress the p53-mediated DNA damage response and NF-κB-mediated apoptotic signaling. Moreover, IR-induced oxidative stress in the colorectal epithelium was markedly reduced by PGE administration. Collectively, this study establishes a mechanism of action by which PGE counteracts IR-induced colorectal injury as a novel radioprotective agent.

특집 II (6) 옥신, 다윈의 호르몬 - 굴광성.굴중성실험 통해 호르몬 존재 유추

조형택,Jo, Hyeong-Taek 한국과학기술단체총연합회 2009 과학과 기술 Vol.42 No.3

운동생리학 : 6주간의 얼음축구 훈련이 호흡순환기능, 기초체력 및 등속성 근력에 미치는 효과

조형택(YoungTaekJo),유근직(KeunJikYoo),곽창수(ChangSooKwak),정갑철(GakChulChung) 한국체육학회 2004 한국체육학회지 Vol.43 No.6

The purpose of this study was to determine the effects of ice-soccer exercises conducted in winter season Subjects employed for this study were 14 persons for ice-soccer group as experimental group and 8 persons for jogging group as control group. Each group was trained for 6 weeks according to each training program, and cardio-respiratory responses, basic physical fitness and isokinetic muscle strength were measured and analyzed at pre and post experiment. The results of this study were as follows ; 1) It was showed that exerted exercise time, heart rate, VO2max, VECO2max did not make statistically significant difference between group, but slightly increased after 6 week training. Basic physical fitness variables showed a little increment, especially agility, flexibility, power(p< .05). 2) Peak torque and total work of both groups increased in the condition of 60°/sec and 180˚/sec angular velocity in flexor, but decreased in the same condition of extensor. The difference between pre & post and two subject groups was not significant. Ice soccer group was the same or higher than jogging group in basic physical fitness, though it was not statistically significant. As a result of this experiment, ice soccer has the same exercise effect likewise jogging popular with the exercise effect. However, further research should be done with the subject on the safety for ice soccer and approximate exercise intensity of sex and age.

해바라기 하배축의 오옥신 유도 신장에서 RNA 및 단백질의 합성과 세포벽 산성화의 관계

조형택(Hyung Taeg Cho),홍영남(Young Nam Hong) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.2

N/A

인슐린 결핍 당뇨 마우스에서 식용곤충 쌍별귀뚜라미 추출물의 항당뇨 활성

조형택(Hyung Taek Cho),심규상(Kyu Sang Sim),김예나(Yeona Kim),장문한(Moon Han Chang),김태완(Taewan Kim),이승환(Seung Hwan Lee),이동희(Dong-Hee Lee),김준호(Jun Ho Kim) 한국식품영양과학회 2019 한국식품영양과학회지 Vol.48 No.10

본 연구에서는 STZ 저용량 반복투여에 의한 인슐린결핍 당뇨 마우스 모델에서 GBE의 항당뇨 활성 및 가능한 작용기전을 검증하였다. 실험기간 동안 GBE 급여는 STZ에 의한 glucose 항상성의 저해를 유의적으로 개선시키며 당뇨 유발을 억제하였으며, 췌장 islet의 형태 및 인슐린분비 기능에 대한 보호 작용을 나타내었다. 또한 GBE의 이러한 보호 효과는 MAPK와 NF-κB 신호전달과정 활성화의 억제와 이로 인한 β-cell apoptosis 저해작용과 연관이 있음을 확인하였다. 이러한 결과는 항당뇨 기능성 식품 소재로써 쌍별귀뚜라미의 활용 가능성에 대한 중요한 학술정보를 제공하며, 향후 식·약용 곤충 소재들의 고부가가치 산업화를 위한 다양한 생리활성 탐색 연구가 지속해서 필요할 것으로 판단된다. The benefits of edible insects as an alternative animal protein source in the future has recently drawn significant attention. This study examined the anti-diabetic potential of edible insect Gryllus bimaculatus in streptozotocin(STZ)-induced insulin-deficient diabetic mice. Diabetes was induced by a multiple-low-dose STZ treatment after two weeks of the oral administration of G. bimaculatus water extracts (GBE) at 150 mg/kg. The administration of GBE reduced the hyperglycemia, delayed the cumulative incidence of diabetes, and improved glucose tolerance in the STZ-treated mice. Moreover, GBE protected the pancreatic islet against STZ-induced disruption of the islet architecture and function by inhibiting β-cell apoptosis in mice. Importantly, these protective effects were associated with the suppression of the mitogen-activated protein kinase- and nuclear factor-kappa B-mediated signaling pathways in the pancreas. These findings provide novel mechanistic insight into the protective effects of GBE against pancreatic islet dysfunction in insulin-deficient diabetes mellitus.

광조건에서 Putrescine 이 잘라낸 배추잎의 노쇠과정에 미치는 효과

조형택(Hyung Taek Cho),홍영남(Young Nam Hong) 한국식물학회 1988 Journal of Plant Biology Vol.31 No.3

Effects of putrescine on senescence in detached leaves of Chinese cabbage (Brassica campestris L.) in the light were investigated. The putrescine as a potent antisenescence substance markedly inhibited chlorophyll and protein loss at the 10 mM concentration in the detached leaves during the dark incubation. In the light, however, putrescine showed the opposite effects to dark incubation. The chlorophyll loss by putrescine in the light was stopped with dark-transfer, and inhibited competitively by a divalent cation Ca^2+. In the light, putrescine reduced the protease activity. Putrescine, in the light, increased H_2O_2 content and reduced the activities of enzymes -superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6)- involved in inhibiting the accumulation of free radicals. These results suggest that the effects of puterscine on chlorophyll and protein loss in detached leaves of Chinese cabbage in the light are related to the cationic nature of putrescine and the accumulation of free radicals. Effects of putrescine on senescence in detached leaves of Chinese cabbage (Brassica campestris L.) in the light were investigated. The putrescine as a potent antisenescence substance markedly inhibited chlorophyll and protein loss at the 10 mM concentration in the detached leaves during the dark incubation. In the light, however, putrescine showed the opposite effects to dark incubation. The chlorophyll loss by putrescine in the light was stopped with dark-transfer, and inhibited competitively by a divalent cation Ca^2+. In the light, putrescine reduced the protease activity. Putrescine, in the light, increased H_2O_2 content and reduced the activities of enzymes -superoxide dismutase (EC 1.15.1.1), peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6)- involved in inhibiting the accumulation of free radicals. These results suggest that the effects of puterscine on chlorophyll and protein loss in detached leaves of Chinese cabbage in the light are related to the cationic nature of putrescine and the accumulation of free radicals.

Branching the auxin signaling; Multiple players and diverse interactions

이민수,최희승,조형택 한국식물학회 2013 Journal of Plant Biology Vol.56 No.3

In contrast to its short signaling pathway, auxin plays a wide spectrum of biological roles during plant growth and development. The nuclear auxin signaling pathway can be described in a single step; auxin/receptor-mediated ubiquitination/degradation of transcriptional repressors that affect the auxin responsive genes. However, this simple signaling step generates not only diverse but also seemingly contrary signaling outputs, such as activation/repression of gene expression and promotion/inhibition of cell growth. This diversity of the nuclear auxin signaling is likely to derive from multiple and diverse players of the signaling components. The major nuclear auxin signaling components are receptors, repressors, DNA-binding transcription factors, and co-repressors. In most land plants, each component of the auxin signaling consists of multiple isoforms with somewhat diversified structures; and thus with diverse molecular functions. The diversity of members of each signaling component is anticipated to contribute to the diverse auxin responses. This study reviews the recent results regarding the diverse molecular structures and interactions among the nuclear auxin signaling players.