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감귤부산물의 사료화에 관한 연구 5 . 면양에 의한 감귤가공 부산물사일리지의 소화율 및 반추위액의 pH 와 total - VFA 농도 변화
양승주,정창기 ( S . J . Yang,C . C . Choung ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2
In order to examine the value of citrus byproducts as livestock feed, 4 types of CBS were made: T1; wet CMS with high moisture. T?; pre-wilted CMS with low moisture. T3; wet CPS with high moisture. T4; pre-wilted CPS with low moisture. Digestion trials were carried with 3 sheep equipped with rumen fistulars. Rumen liquor pH and total VFA were investigated during the trial period. The daily intake of the trial feed (CBS+hay) was 0.76-0.78㎏ DM (1.8-1.9% by body wt.). Feeding CBS showed the effect of increasing the feed. intake. Compared with high moisture CBS, low moisture CBS increased feed intake and digestibility, and was weak in the tendency of lowering rumen liquor pH. Compared with CPS, CMS showed a tendency more similar to low moisture CBS. Feeding CBS had the effect of increasing urine excretion, water intake and digestibility of DM and DNFE. When fed CBS the pH and total VFA in rumen liquor were lower than when fed hay. Such tendency was shown in the case of feeding high moisture CBS compared to low moisture CBS. The digestibility of CBS had no relationship with pH, but there was a statistical significant correlation between total VFA in rumen liquor and digestibility of CBS. It appears from the results of these trials in the making and utilization of CBS that CMS was better in all respects than CPS and prewilted CBS with low moisture (about 76%) was more effective than wet CBS with high moisture (about 83%).
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포유자돈 선천성 진전증 증례에서 atypical porcine pestivirus (APPV) 검출
김승채,정창기,윤승민,이기호,양면식,김범석,이승윤,강석진,김원일,Kim, Seung-Chai,Jeong, Chang-Gi,Yoon, Seung-Min,Lee, Ki-Ho,Yang, Myoun-Sik,Kim, Bumseok,Lee, Seung-Yoon,Kang, Seog-Jin,Kim, Won-Il 한국동물위생학회 2017 한국동물위생학회지 (KOJVS) Vol.40 No.3
Congenital tremor (CT) is a sporadic neurodegenerative disease reported in suckling piglets worldwide. Since atypical porcine pestivirus (APPV) was first identified in US in 2015, it has also subsequently detected in Europe and China as a causative pathogen for CT in suckling piglets. Three new-born piglets died from severe tremor was submitted to Chonbuk National University-Veterinary Diagnostic Center (CBNU-VDC) and various tissues (lung, lymph node, brain, intestine) were tested with panpestivirus RT-PCR and APPV NS5B-specific RT-PCR. All of the samples were positive by both of the PCR tests and the partial NS5B sequences of APPV were confirmed by sequencing on the PCR products of APPV NS5B-specific RT-PCR. Therefore, we report the first identification of APPV from a case of CT in suckling piglets in Korea.
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Evaluation of different media for ex vivo porcine lung culture model
Myeon-Sik Yang,Zixiong Zhou,Amina Khatun,Salik Nazki,정창기,김원일,이상명,Seog-Jin Kang,임채웅,김범석 한국동물위생학회 2018 한국동물위생학회지 (KOJVS) Vol.41 No.4
Developing drugs targeting respiratory pathogen is essential to control respiratory diseases. Many experi-ments have been performed under in vivo situation. However, in vivo experiments have economical and ethical issues. The objective of this study was to determine the possibility of developing an ex vivo lung culture system with possible application for respiratory infection studies. After isolating lungs from naïve pigs, agarose-inflated lung tissues were prepared and sliced manually. These sliced lung tissues were then subsequently placed on 24-well plates. Eight different combinations of media were used to determine the optimum ex vivo lung culture condition. In addition, lung tissues were infected with por-cine reproductive and respiratory syndrome (PRRS) virus at a titer of 1×104 TCID50/mL. Virus growth was confirmed by titration in MARC-145 cells at 2, 4, 6 days post infection (dpi). We found that ex vivo lung culture in physiological environment was not media specific based on histopathology and cytotoxicity. However, under virus-infected condition, thickened alveolar walls in the lung tissues and stable virus titers at 2, 4, 6 dpi were shown in F12K medium suggesting that it was useful for tissue maintenance and virus infection using PRRS virus infected lung tissues. The present study shows the possibility of using porcine ex vivo lung model for respiratory infection studies.
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Myeon-Sik Yang,정창기,Salik Nazki,Sameer ul Salam Mattoo,Sang-Myeong Lee,Won-Il Kim,Bumseok Kim 한국동물위생학회 2019 한국동물위생학회지 (KOJVS) Vol.42 No.4
Porcine reproductive and respiratory syndrome (PRRS) is characterized by reproductive failure in sows and respiratory distress in all age pigs. Porcine respiratory disease complex (PRDC) is a disease caused by opportunistic bacterial infection secondary to a weakened immune system by a preceding respiratory infection. In this study, we tried to compare the immune responses in PRRS and PRDC groups to clearly characterize the disease severity. Eighty-five pigs were infected with various Korean field PRRS virus strains. Infected animals were classified into PRRS (n=32) and PRDC (n=53) groups based on lung le-sions such as interstitial pneumonia, suppurative pneumonia, and pleuropneumonia. The immune cell population of bronchoalveolar lavage cells (BALc) was evaluated on 14 and 28 days post infection (dpi) and PMBC cytokine expression was measured on 0, 3, 7, 14 dpi to investigate early inflammatory re-actions. Pulmonary lesion severity was negatively correlated with alveolar macrophage (AM) in both PRRS and PRDC groups on 14 and 28 dpi. AM in BALc was less populated in PRDC group on 28 dpi compared to PRRS group. AM in BALc was significantly less populated in PRDC group on 28 dpi compared to 14 dpi. In addition, cytotoxic T lymphocyte (CTL) in BALc was higher populated in PRDC group on 14 dpi and 28 dpi compared to PRRS group. In the case of PBMC cytokine TNF-, IFN-, IL-1, IFN-, FoxP3, and IL-2, the PRRS group showed higher expression than the PRDC group on 7 dpi, 14 dpi, 7 dpi, 14 dpi, 14 dpi, and 14 dpi, respectively. On the other hand, in the case of IFN-, IL-6, IL-8, IL-4, and IL-17, the PRDC group showed higher PBMC cytokine expression at 14 dpi, 7 dpi, 14 dpi, 3 dpi, and 3 dpi, respectively, than the PRRS group. Based on these results, our study could characterize differential immune responses in pigs with PRRS or PRDC.
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오응세(Oh Eung-Se),양승옥(Yang Sung-Ok),정창기(Jung Chang-Ki) 대한전기학회 2008 대한전기학회 학술대회 논문집 Vol.2008 No.10
산업현장의 이더넷 통신망의 적용은 CSMA/CD 매체접근 방식에서 발생가능한 충돌회피 알고리듬의 비결정론적인 특성으로 인해 많은 논란이 있었지만, 전이중(full duplex) 방식의 스위치(switch)의 도입으로 충돌이 회피됨으로써 이러한 논의는 이제 거의 불식된 것 같다. 따라서 과거와 같이 공유 전송망에서 고려되던 최대 통신부하는 이제 각 단위 통신망의 최대 대역폭의 능력으로 직접적인 유추가 가능하게 되었다. 이에 과거의 매체공유방식의 분산제어시스템에서 사용되던 통신망의 전송부하 시험을 대체할 용이한 통신부하 시험 모델을 제안하고자 한다.
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