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정명철,전효곤,이호재,고영희,Chung, Myung-Chul,Chun, Hyo-Kon,Lee, Ho-Jae,Kho, Yung-Hee 한국미생물 · 생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.5
The strain SL-387 which produces new inhibitors of aminopeptidase M, MR-387A and B, was isolated from a soil sample. The strain has branched substrate mycelia, from which aerial hyphae develop in the form of open spirals. Spore surface is smooth. Melanoid and soluble pigme- nts were observed. The isolate contains LL-diaminopimelic acid in its cell wall hydrolysate, and has no pectinolytic activity. The strain SL-387 is closely related to Streptomyces griseoruber and S. naganishii, but is different from these strains in some cultural and physiological characteristics. This strain was, therefore, designated as Streptomyces sp. SL-387. The effects of several carbon and nitrogen sources on the production of the inhibitor were examined. Among them, glucose, galactose, mannose, and xylose were effective as a carbon source and soybean meal, soytone, fish meal, and gluten meal were effective as a nitrogen source. The maximum peak of the inhibitor production in jar fermentor was obtained on the fifth day of culture.
무릇에서 분리한 nortriterpenoid glycoside의 암세포에 대한 세포독성 및 함량 분석
이상명,전효곤,이충환,이호재,강진정,맹학영,고영희,Lee, Sang-Myung,Chun, Hyo-Kon,Lee, Choong-Hwan,Lee, Ho-Jae,Kang, Shin-Jyung,Maeng, Hack-Young,Kho, Yung-Hee 한국생약학회 2001 생약학회지 Vol.32 No.3
Scillascilloside E-3 (1) and E-1 (2) were isolated from the bulbs of Scilla scilloides. The cytotoxic activities of these compounds were tested against murine (B16/F-10, 3LL) and human cancer cell lines (MCF7, PC-3, HT29, LOX-IMVI, A549 and HT1080). These compounds exhibited a significant cytotoxic activities against all tested cancer cells. Futhermore, the contents of 1 and 2 in S. scilloides are 43.2 and 27.9 mg/kg, respectively.
Streptomyces sp . SL - 387 에 의한 Aminopeptidase M 저해제 MR - 387A 및 B 의 생산 배지 최적화
정명철(Myung Chul Chung),전효곤(Hyo Kon Chun),이호재(Ho Jae Lee),이충환(Choong Hwan Lee),김수일(Su Il Kim),고영희(Yung Hee Kho) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2
Media optimization for the production of MR-387A and B, novel aminopeptidase M inhibitors by Streptomyces sp. SL-387 isolated from a soil was studied. Optimized medium was consisted of 1% glucose, 3% soybean meal, 0.2% yeast extract, 0.1% beef extract, 0.3% NaCl, 0.01% K₂HPO₄, 0.3% CaCO₃. 0.001% MnCl₂·4H₂O, 0.001% ZnCl₂·7H₂O, and 0.0005% MgSO₄·7H₂O, and adjusted to pH 7.0 before autoclaving. When the optimized medium was used as a fermentation medium, maximum productivity of MR-387 was reached at 120 hours of fermentation, and total productivity was 909.1 U/㎖.
Inhibition of Matrix Metalloproteinase-2 Activity of Flavonol Glycosides from Cedreia sinensis
황선우,하태정,고영희,전효곤,이준,권현숙,박기훈,양민석,Hwang Seon-Woo,Ha Tae-Joung,Kho Yung-Hee,Chun Hyo-Kon,Lee Jun,Kwon Hyun-Sook,Park Ki-Hun,Yang Min-Suk Korean Society of Life Science 2006 생명과학회지 Vol.16 No.3
Cedrela sinensis is a broadleaf tree that is widely cultivated in Korea and China. It was used for treating enteritis, dysentery, and skin itch in oriental medicine. In this study, three major flavonoids, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2), and quercetin-3-O-glucoside (3), were isolated from the leaf of Cedrela sinensis. The biological activities of these compounds were tested by inhibitory activity of matrix metalloproteinases-2 (Type IV collagenase) method together with a cytotoxicity and a apoptosis test against human cancer cell lines. 참죽나무는 멀구슬과 나무로서 한국과 중국에 널리 분포하고 있다. 한방에서는 장염(腸炎), 이질(痢疾), 개선(疥癬) 등의 치료에 이용되고 있다. 본 연구는 참죽나무 잎에서 3종의 flavonol glycosides를 분리하여 NMR을 통해 구조를 확인한 결과, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2)와 quercetin-3-O-glucoside(3)로 구조 동정되었다. 분리된 화합물들의 생리활성은 matrix metalloproteinase-2 저해 활성과 인체암 세포주에 대한 세포독성과 apoptosis실험을 통하여 항암효과를 조사하였다. 그 결과 인체암 세포주에 대한 세포독성과 apoptosis 저해활성은 나타내지 않았으나, MMP-2활성저해 조사에서는 강한 저해 효과를 나타내었다. 특히 quercetin-3-O-glucoside는 암세포에 직접적인 독성을 보이는 것이 아니라 암의 침윤과 전이에 특이적으로 작용하는 물질로 보여진다.